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The map label for this gene is ada [H]

Identifier: 78066390

GI number: 78066390

Start: 1941858

End: 1942451

Strand: Direct

Name: ada [H]

Synonym: Bcep18194_A4920

Alternate gene names: 78066390

Gene position: 1941858-1942451 (Clockwise)

Preceding gene: 78066389

Following gene: 78066391

Centisome position: 52.57

GC content: 56.06

Gene sequence:

>594_bases
TTGTCGCATACCTTATCAAAAAGGAGATCTGCCATGAAGAACCGTCCTTCTACTGCTTTCAAGTACAACTTCCGCGCTCC
GGGCAGCGACGTCGAAGGCCCAATTCGTTATGCGATTGGAAAGACCGTCCGCGAAATGATTCTGGTCGGAAAAAGTCGCC
GCGGAATCTGCGCGATCTTTCTTGGTGATTCGCATCAAGCGTTGGTCGATGAACTTGAGGCGGAGTTTCCGCATAATCCT
CTAGAGACGGACCAGCTTGGACTGCACCGTGAGTTGGTCCAGGTCATCGCGTTCATTGACAAGGATATTGCCGAAGGGGT
CATCGATCTCGACATTGGCGGCACGCCTTTCCAGCAGCAGGTGTGGCAGGCATTGTGTGGCATCCCTGCCGGTCAGACTC
GTAGCTATGGCGAGGTAGCACGAGACCTCGGCGTACCTGAGGCGGTTCGTGCTGTCGCAAGCGCATGCGCGGCGAACGTG
CTTGCAGTAGCGATTCCGTGTCACCGAGTCGTTCGTCGCGATGGATCGGTATCCGGTTATCGCTGGGGGGTGGATCGCAA
GCGTGCACTGCTCTCGAAAGAGAGCGAGCAATGA

Upstream 100 bases:

>100_bases
GATCTGGCTGCGATTGATCGCCCGATTTCCGGGATGGTGATTGGGAAATCGAATTTGACCGCAAGGACCAGAGTTTGGAG
ACGTCGATCAGTGCCTAAAC

Downstream 100 bases:

>100_bases
GCACGTTCGACAAGGACCGCTACTGGTCTGTGGATGGGCGCGAAATCCTCGGGGAGAACGCATTCATTCTCCGAGGATTT
GCGCTGCCCGGCGCGGGCGA

Product: DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSHTLSKRRSAMKNRPSTAFKYNFRAPGSDVEGPIRYAIGKTVREMILVGKSRRGICAIFLGDSHQALVDELEAEFPHNP
LETDQLGLHRELVQVIAFIDKDIAEGVIDLDIGGTPFQQQVWQALCGIPAGQTRSYGEVARDLGVPEAVRAVASACAANV
LAVAIPCHRVVRRDGSVSGYRWGVDRKRALLSKESEQ

Sequences:

>Translated_197_residues
MSHTLSKRRSAMKNRPSTAFKYNFRAPGSDVEGPIRYAIGKTVREMILVGKSRRGICAIFLGDSHQALVDELEAEFPHNP
LETDQLGLHRELVQVIAFIDKDIAEGVIDLDIGGTPFQQQVWQALCGIPAGQTRSYGEVARDLGVPEAVRAVASACAANV
LAVAIPCHRVVRRDGSVSGYRWGVDRKRALLSKESEQ
>Mature_196_residues
SHTLSKRRSAMKNRPSTAFKYNFRAPGSDVEGPIRYAIGKTVREMILVGKSRRGICAIFLGDSHQALVDELEAEFPHNPL
ETDQLGLHRELVQVIAFIDKDIAEGVIDLDIGGTPFQQQVWQALCGIPAGQTRSYGEVARDLGVPEAVRAVASACAANVL
AVAIPCHRVVRRDGSVSGYRWGVDRKRALLSKESEQ

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=174, Percent_Identity=50, Blast_Score=163, Evalue=5e-42,
Organism=Escherichia coli, GI1787596, Length=82, Percent_Identity=50, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=129, Percent_Identity=42.6356589147287, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=126, Percent_Identity=43.6507936507937, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=40.4761904761905, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137554, Length=181, Percent_Identity=33.7016574585635, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21595; Mature: 21464

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTLSKRRSAMKNRPSTAFKYNFRAPGSDVEGPIRYAIGKTVREMILVGKSRRGICAIF
CCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
LGDSHQALVDELEAEFPHNPLETDQLGLHRELVQVIAFIDKDIAEGVIDLDIGGTPFQQQ
ECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHH
VWQALCGIPAGQTRSYGEVARDLGVPEAVRAVASACAANVLAVAIPCHRVVRRDGSVSGY
HHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
RWGVDRKRALLSKESEQ
ECCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SHTLSKRRSAMKNRPSTAFKYNFRAPGSDVEGPIRYAIGKTVREMILVGKSRRGICAIF
CCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
LGDSHQALVDELEAEFPHNPLETDQLGLHRELVQVIAFIDKDIAEGVIDLDIGGTPFQQQ
ECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHH
VWQALCGIPAGQTRSYGEVARDLGVPEAVRAVASACAANVLAVAIPCHRVVRRDGSVSGY
HHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
RWGVDRKRALLSKESEQ
ECCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609 [H]