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The map label for this gene is yafC [H]

Identifier: 78066385

GI number: 78066385

Start: 1937980

End: 1938882

Strand: Reverse

Name: yafC [H]

Synonym: Bcep18194_A4915

Alternate gene names: 78066385

Gene position: 1938882-1937980 (Counterclockwise)

Preceding gene: 78066386

Following gene: 78066384

Centisome position: 52.49

GC content: 63.9

Gene sequence:

>903_bases
ATGCTGGATCTCAACGACTTTCGCTATTTCGTCCGAATCGTCGAATCGGGAGGACTCACGGCGGCCAGCCGGAACCTCGA
CATCCCGAAATCGACCGTCAGCCACCGGCTTCAGCAGCTGGAAGCCGCGCTCGGCGTGCGGCTGATCAACCGAACATCGC
GCAGCTTAAGCGTGACCGACGCAGGCCAGCTCTTCTACAGTCACGCCGTCACAATGCTGGAGCGCGCCGAAGTAGCCGAG
AACGCCGTCCGCGAGCGCCTGGTCGAACCGATCGGCACGATCCGGTTCACGACTGCGGTGGCCACGTCGCTCTACGCGAT
GCGCCAGATCCTTCCGGATTTCATCAAGCGTTACCCGAAAATCGACGTGATCGAGCACGTCAGCGACGAACAGACCGACA
TCGTCGGCGGCAATTTCGATCTTGCGCTGCGTGCCCATAACGGGCCGCTATCCGACTCCGCGCTCGTGCAACGCAAGCTG
GCCCCTGCCCCGTGGTTCCTATTCGCCAGCCCGGACTATCTCGACCGACGTGGCCAGCCCGTCGCGCCGGACGAGCTGGC
CGGGCACGATTCGCTGTTCGTCACAAGGCACCATCTTCCGTTCGCCTGGAAGCTCAAGCATCCGTCGCAGGGTGAAGTCA
GCGTCCCGCTGAAGCCACGCCTCGCAGGCAACGACTTGGTCACGCTGAAACAGGCCGCGCAGGATGGACTCGGCGTCGTC
GCGCTCCCTGGCTATGTCTGTCGCGACGAAGTCCGCTCCGGCGCGCTGCGCTGCATTCTGCCCGATTGGCTCGCCGGCGA
AGCCACCATCACCGCCGTACTGCCGTTCCGGCACGGCATGCTGCCGTCGGTGCGCGTGTTTCTCGACTTCCTGGTTGAGC
GCATCCCAAAGGTCGTGTCGTAG

Upstream 100 bases:

>100_bases
ATTTCGCACGAAATGTACTTATCGACCCAGTCGGCATACGTTCCAGTAGGAAGACGACTAGAATCAGATCGTCCAATTGA
TCGAACCATACGGATCAAAC

Downstream 100 bases:

>100_bases
CATCATCGTCCAAACTATTGGACATTAATTCCATAGCATCCATCTTGTTGATCCATACGTCTGAATCCATTCTGTAGTTA
CGGCCGGTCGATACCGATCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLDLNDFRYFVRIVESGGLTAASRNLDIPKSTVSHRLQQLEAALGVRLINRTSRSLSVTDAGQLFYSHAVTMLERAEVAE
NAVRERLVEPIGTIRFTTAVATSLYAMRQILPDFIKRYPKIDVIEHVSDEQTDIVGGNFDLALRAHNGPLSDSALVQRKL
APAPWFLFASPDYLDRRGQPVAPDELAGHDSLFVTRHHLPFAWKLKHPSQGEVSVPLKPRLAGNDLVTLKQAAQDGLGVV
ALPGYVCRDEVRSGALRCILPDWLAGEATITAVLPFRHGMLPSVRVFLDFLVERIPKVVS

Sequences:

>Translated_300_residues
MLDLNDFRYFVRIVESGGLTAASRNLDIPKSTVSHRLQQLEAALGVRLINRTSRSLSVTDAGQLFYSHAVTMLERAEVAE
NAVRERLVEPIGTIRFTTAVATSLYAMRQILPDFIKRYPKIDVIEHVSDEQTDIVGGNFDLALRAHNGPLSDSALVQRKL
APAPWFLFASPDYLDRRGQPVAPDELAGHDSLFVTRHHLPFAWKLKHPSQGEVSVPLKPRLAGNDLVTLKQAAQDGLGVV
ALPGYVCRDEVRSGALRCILPDWLAGEATITAVLPFRHGMLPSVRVFLDFLVERIPKVVS
>Mature_300_residues
MLDLNDFRYFVRIVESGGLTAASRNLDIPKSTVSHRLQQLEAALGVRLINRTSRSLSVTDAGQLFYSHAVTMLERAEVAE
NAVRERLVEPIGTIRFTTAVATSLYAMRQILPDFIKRYPKIDVIEHVSDEQTDIVGGNFDLALRAHNGPLSDSALVQRKL
APAPWFLFASPDYLDRRGQPVAPDELAGHDSLFVTRHHLPFAWKLKHPSQGEVSVPLKPRLAGNDLVTLKQAAQDGLGVV
ALPGYVCRDEVRSGALRCILPDWLAGEATITAVLPFRHGMLPSVRVFLDFLVERIPKVVS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=31.7073170731707, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1787128, Length=301, Percent_Identity=25.5813953488372, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=26.4214046822742, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=28.9655172413793, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=26.6891891891892, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=26.2975778546713, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787589, Length=247, Percent_Identity=29.1497975708502, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787601, Length=178, Percent_Identity=25.8426966292135, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788481, Length=261, Percent_Identity=26.4367816091954, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788887, Length=308, Percent_Identity=26.6233766233766, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788297, Length=264, Percent_Identity=26.5151515151515, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=126, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788748, Length=175, Percent_Identity=26.2857142857143, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=24.3243243243243, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33217; Mature: 33217

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50931 HTH_LYSR ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLNDFRYFVRIVESGGLTAASRNLDIPKSTVSHRLQQLEAALGVRLINRTSRSLSVTD
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
AGQLFYSHAVTMLERAEVAENAVRERLVEPIGTIRFTTAVATSLYAMRQILPDFIKRYPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IDVIEHVSDEQTDIVGGNFDLALRAHNGPLSDSALVQRKLAPAPWFLFASPDYLDRRGQP
CCHHHHCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCHHHHCCCCC
VAPDELAGHDSLFVTRHHLPFAWKLKHPSQGEVSVPLKPRLAGNDLVTLKQAAQDGLGVV
CCCHHHCCCCCEEEEECCCCEEEEECCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCEE
ALPGYVCRDEVRSGALRCILPDWLAGEATITAVLPFRHGMLPSVRVFLDFLVERIPKVVS
ECCCHHHHHHHHCCCEEEECCCHHCCCEEEEEEECHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDLNDFRYFVRIVESGGLTAASRNLDIPKSTVSHRLQQLEAALGVRLINRTSRSLSVTD
CCCHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECH
AGQLFYSHAVTMLERAEVAENAVRERLVEPIGTIRFTTAVATSLYAMRQILPDFIKRYPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IDVIEHVSDEQTDIVGGNFDLALRAHNGPLSDSALVQRKLAPAPWFLFASPDYLDRRGQP
CCHHHHCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCHHHHCCCCC
VAPDELAGHDSLFVTRHHLPFAWKLKHPSQGEVSVPLKPRLAGNDLVTLKQAAQDGLGVV
CCCHHHCCCCCEEEEECCCCEEEEECCCCCCCEECCCCCCCCCCCHHHHHHHHHCCCCEE
ALPGYVCRDEVRSGALRCILPDWLAGEATITAVLPFRHGMLPSVRVFLDFLVERIPKVVS
ECCCHHHHHHHHCCCEEEECCCHHCCCEEEEEEECHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]