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The map label for this gene is dhbE [H]

Identifier: 78066370

GI number: 78066370

Start: 1918283

End: 1919968

Strand: Direct

Name: dhbE [H]

Synonym: Bcep18194_A4900

Alternate gene names: 78066370

Gene position: 1918283-1919968 (Clockwise)

Preceding gene: 78066369

Following gene: 78066371

Centisome position: 51.93

GC content: 65.42

Gene sequence:

>1686_bases
ATGACGGGCATTCATTTTCCGGTCGACGGTGTCGTCTATTGCGATCCGGCCGACGCGCAGCGTTACCTGCAGGCCGGTGC
GTGGATCGATCGCACGTTCGGCGAGGCACTGGCGGAGACGGCGCGACGGCTTCCGGACAAGGCCGCATTCATTGCCGACG
GCCGGACGCTGACGTTTCGCGAGCTGGACGAAGAGAGTGATCGCCTCGCAGCGGCACTCGTGCGTCTCGGGCTGAAGCCG
GGCACGCGCGCGATGTTCCAGATGGGCACGACCCTCGATACCGCGCTCGCGTTGTGCGCCTGCTACAAGAGCGGCGTCGT
GCCGGTCTGTTCGCTGCCGCAATACCGCGAAGTCGAGATCGGCAAGCTCGCCGACCTCGCCCGGCCGGAAGCCTACTTCG
TGCAGGCGGATATCGGCCGCTTCGATCTCGTCGAGTTTGCGCAGACGATGTGCCGCGAACACGCATCCTTCCGGCATCTG
ATCGTCGCGCGCGGCGAAGCGCTGGCCGGCGCGCAGTCGATGTCCGAGCTGACGTCGTCGCTTTCGCTCGACGAAGCGCG
GCGGGTGCTGGCGGACGTGCGGATCGGATCCGAGGACGTGCTGAGCTTCCAGTTGTCGGGCGGCACGACGGGCGTGCCGA
AGATCATCCCGCGCTTTCATGCGGAATACCTCGGTCATTCGCTGGCGTGGTCGCGTCACGTCAACGGCGGCGAGCAGGCC
ACGCTGATCTGGTCGCTGCCATTGCTGCACAACGCGGCGCACCTGTACGCGCTCGTGCCGACGATCGCGGCAGGGCAGAC
CACGATCCTGATGTCGTCCGTCGACGTGGTGCAGATGGCCCGGCTGATCGAGCAGCATCGCGCGACGCATGCGGTGTCGA
TCGGCCCGGTGGCGCCGCAGATCATGGCGAATCCGCAGGTGCTCGACCACGATCTGTCGTCGCTGAAGCTGTTCTTCTGC
CTGACGCGCGCCGACAACCTCGAAGCGTATCTCGGTGTGCCGTGCTCGAACATGTACGGCACCACGGAAGGGTTGCTGAT
CGGATCCGGCGGCACCGTCGACGAGCACGTACGCCATCACACGCACGGCCGCTCGGGCTGCGACCAGGACGAACTCGTGC
TGCTCGAGCCAGATAGCGAGATACCGGTTCCCACCGGGCAGGTCGGCGAACTCTGCTTTCGCGGTCCGTCCAGCCTGCGC
GGCTATTTCAGTGCGCCGGAAGCCAACGCGACGGCCTTTACGAAGGACGGTTTCTTCCGTACCGGCGACATGATGCGGGC
GCACGTGATCGACGGCGCGACGTATTTCTCGTTCGAGGGCCGCCTGCGCGACAACATCAATCGCGGCGGCGAAAAGATCG
GCGCGGAAGAAGTCGAGGCATTCCTGAGCCATCACCCGGCGGTGCTCGACGCGAAGCTGGTCGCGATGCCAGATCCGGTC
TATGGCGAGAAGGGCTGCGCGTTCCTGATCCTGCGGCCGGGACACGTCCCCCCGACGATCCCGGAACTGATCGACTTCCT
CGGCACACAGGGCCTCGCGAAGTTCAAGTGCCCGGAGCGCGTCGAAGTGGTGGACGAGTTTCCAGTGACCCGGGTCGGCA
AGGTCGACAAGCCGGCCATGCGACGCGCGATTGCCGCCATTCTTGAAACAGAACAGTTGTCCCATACCCCCAGGCAGGAG
CAATGA

Upstream 100 bases:

>100_bases
TACGGGCTCTTCACCGGCGGCTTGCGCGGCAGCATCGACGTGTTCGACAAAGACCGCGTGCGCGTCGTGGCCGCGTAGCC
CGGGACAGGAGGAGACAACC

Downstream 100 bases:

>100_bases
CATGAGCCAGGTCAGCTATCGAATCGGCCAGATCGTGCCCAGTTCGAACACGACGATGGAAACGGAAGTGCCGGCGCTGC
TGCGCCGCCGCGAACTCGTC

Product: AMP-dependent synthetase and ligase

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MTGIHFPVDGVVYCDPADAQRYLQAGAWIDRTFGEALAETARRLPDKAAFIADGRTLTFRELDEESDRLAAALVRLGLKP
GTRAMFQMGTTLDTALALCACYKSGVVPVCSLPQYREVEIGKLADLARPEAYFVQADIGRFDLVEFAQTMCREHASFRHL
IVARGEALAGAQSMSELTSSLSLDEARRVLADVRIGSEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSLAWSRHVNGGEQA
TLIWSLPLLHNAAHLYALVPTIAAGQTTILMSSVDVVQMARLIEQHRATHAVSIGPVAPQIMANPQVLDHDLSSLKLFFC
LTRADNLEAYLGVPCSNMYGTTEGLLIGSGGTVDEHVRHHTHGRSGCDQDELVLLEPDSEIPVPTGQVGELCFRGPSSLR
GYFSAPEANATAFTKDGFFRTGDMMRAHVIDGATYFSFEGRLRDNINRGGEKIGAEEVEAFLSHHPAVLDAKLVAMPDPV
YGEKGCAFLILRPGHVPPTIPELIDFLGTQGLAKFKCPERVEVVDEFPVTRVGKVDKPAMRRAIAAILETEQLSHTPRQE
Q

Sequences:

>Translated_561_residues
MTGIHFPVDGVVYCDPADAQRYLQAGAWIDRTFGEALAETARRLPDKAAFIADGRTLTFRELDEESDRLAAALVRLGLKP
GTRAMFQMGTTLDTALALCACYKSGVVPVCSLPQYREVEIGKLADLARPEAYFVQADIGRFDLVEFAQTMCREHASFRHL
IVARGEALAGAQSMSELTSSLSLDEARRVLADVRIGSEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSLAWSRHVNGGEQA
TLIWSLPLLHNAAHLYALVPTIAAGQTTILMSSVDVVQMARLIEQHRATHAVSIGPVAPQIMANPQVLDHDLSSLKLFFC
LTRADNLEAYLGVPCSNMYGTTEGLLIGSGGTVDEHVRHHTHGRSGCDQDELVLLEPDSEIPVPTGQVGELCFRGPSSLR
GYFSAPEANATAFTKDGFFRTGDMMRAHVIDGATYFSFEGRLRDNINRGGEKIGAEEVEAFLSHHPAVLDAKLVAMPDPV
YGEKGCAFLILRPGHVPPTIPELIDFLGTQGLAKFKCPERVEVVDEFPVTRVGKVDKPAMRRAIAAILETEQLSHTPRQE
Q
>Mature_560_residues
TGIHFPVDGVVYCDPADAQRYLQAGAWIDRTFGEALAETARRLPDKAAFIADGRTLTFRELDEESDRLAAALVRLGLKPG
TRAMFQMGTTLDTALALCACYKSGVVPVCSLPQYREVEIGKLADLARPEAYFVQADIGRFDLVEFAQTMCREHASFRHLI
VARGEALAGAQSMSELTSSLSLDEARRVLADVRIGSEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSLAWSRHVNGGEQAT
LIWSLPLLHNAAHLYALVPTIAAGQTTILMSSVDVVQMARLIEQHRATHAVSIGPVAPQIMANPQVLDHDLSSLKLFFCL
TRADNLEAYLGVPCSNMYGTTEGLLIGSGGTVDEHVRHHTHGRSGCDQDELVLLEPDSEIPVPTGQVGELCFRGPSSLRG
YFSAPEANATAFTKDGFFRTGDMMRAHVIDGATYFSFEGRLRDNINRGGEKIGAEEVEAFLSHHPAVLDAKLVAMPDPVY
GEKGCAFLILRPGHVPPTIPELIDFLGTQGLAKFKCPERVEVVDEFPVTRVGKVDKPAMRRAIAAILETEQLSHTPRQEQ

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=587, Percent_Identity=23.679727427598, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI38505220, Length=528, Percent_Identity=24.8106060606061, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI157311624, Length=537, Percent_Identity=24.7672253258845, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI157311622, Length=537, Percent_Identity=24.7672253258845, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI58082049, Length=539, Percent_Identity=24.6753246753247, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI187761345, Length=155, Percent_Identity=38.0645161290323, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI187761343, Length=155, Percent_Identity=38.0645161290323, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI28416953, Length=578, Percent_Identity=24.7404844290657, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI115511026, Length=528, Percent_Identity=23.2954545454545, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI122937307, Length=533, Percent_Identity=23.4521575984991, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI42544132, Length=545, Percent_Identity=24.2201834862385, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786810, Length=547, Percent_Identity=33.45521023766, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI145693145, Length=537, Percent_Identity=28.1191806331471, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1788107, Length=563, Percent_Identity=24.5115452930728, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI221142682, Length=500, Percent_Identity=23.2, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1786529, Length=565, Percent_Identity=23.5398230088496, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17560308, Length=562, Percent_Identity=26.3345195729537, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=565, Percent_Identity=25.8407079646018, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71994703, Length=565, Percent_Identity=25.8407079646018, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71994690, Length=565, Percent_Identity=25.8407079646018, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=525, Percent_Identity=23.2380952380952, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=542, Percent_Identity=24.169741697417, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=534, Percent_Identity=23.9700374531835, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=520, Percent_Identity=23.4615384615385, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=541, Percent_Identity=21.2569316081331, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=545, Percent_Identity=23.4862385321101, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560140, Length=547, Percent_Identity=22.3034734917733, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=357, Percent_Identity=28.0112044817927, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323182, Length=579, Percent_Identity=23.3160621761658, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24581924, Length=570, Percent_Identity=25.2631578947368, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21355181, Length=559, Percent_Identity=23.2558139534884, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21358303, Length=503, Percent_Identity=23.0616302186879, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18859661, Length=508, Percent_Identity=23.4251968503937, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922652, Length=471, Percent_Identity=22.5053078556263, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62472339, Length=540, Percent_Identity=21.8518518518519, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24667955, Length=558, Percent_Identity=21.8637992831541, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28573631, Length=571, Percent_Identity=23.9929947460595, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI20130353, Length=571, Percent_Identity=24.1681260945709, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24667959, Length=380, Percent_Identity=24.4736842105263, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281365686, Length=486, Percent_Identity=23.4567901234568, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 61138; Mature: 61007

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIHFPVDGVVYCDPADAQRYLQAGAWIDRTFGEALAETARRLPDKAAFIADGRTLTFR
CCCCEECCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEHH
ELDEESDRLAAALVRLGLKPGTRAMFQMGTTLDTALALCACYKSGVVPVCSLPQYREVEI
HCCCHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
GKLADLARPEAYFVQADIGRFDLVEFAQTMCREHASFRHLIVARGEALAGAQSMSELTSS
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHH
LSLDEARRVLADVRIGSEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSLAWSRHVNGGEQA
CCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCE
TLIWSLPLLHNAAHLYALVPTIAAGQTTILMSSVDVVQMARLIEQHRATHAVSIGPVAPQ
EEEEECHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCEEEECCCCHH
IMANPQVLDHDLSSLKLFFCLTRADNLEAYLGVPCSNMYGTTEGLLIGSGGTVDEHVRHH
HHCCCHHHHHHHHHEEEEHEEECCCCCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHH
THGRSGCDQDELVLLEPDSEIPVPTGQVGELCFRGPSSLRGYFSAPEANATAFTKDGFFR
CCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCCEEEECCCCEE
TGDMMRAHVIDGATYFSFEGRLRDNINRGGEKIGAEEVEAFLSHHPAVLDAKLVAMPDPV
CCCCEEEEEECCEEEEEECCHHHHHHCCCHHHCCHHHHHHHHHCCCCCCCEEEEECCCCC
YGEKGCAFLILRPGHVPPTIPELIDFLGTQGLAKFKCPERVEVVDEFPVTRVGKVDKPAM
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHHCCCCCCCCCCCHHHH
RRAIAAILETEQLSHTPRQEQ
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TGIHFPVDGVVYCDPADAQRYLQAGAWIDRTFGEALAETARRLPDKAAFIADGRTLTFR
CCCEECCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEHH
ELDEESDRLAAALVRLGLKPGTRAMFQMGTTLDTALALCACYKSGVVPVCSLPQYREVEI
HCCCHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
GKLADLARPEAYFVQADIGRFDLVEFAQTMCREHASFRHLIVARGEALAGAQSMSELTSS
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHH
LSLDEARRVLADVRIGSEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSLAWSRHVNGGEQA
CCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCE
TLIWSLPLLHNAAHLYALVPTIAAGQTTILMSSVDVVQMARLIEQHRATHAVSIGPVAPQ
EEEEECHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCEEEECCCCHH
IMANPQVLDHDLSSLKLFFCLTRADNLEAYLGVPCSNMYGTTEGLLIGSGGTVDEHVRHH
HHCCCHHHHHHHHHEEEEHEEECCCCCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHH
THGRSGCDQDELVLLEPDSEIPVPTGQVGELCFRGPSSLRGYFSAPEANATAFTKDGFFR
CCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHCCCHHHCCCCCCCCCCCEEEECCCCEE
TGDMMRAHVIDGATYFSFEGRLRDNINRGGEKIGAEEVEAFLSHHPAVLDAKLVAMPDPV
CCCCEEEEEECCEEEEEECCHHHHHHCCCHHHCCHHHHHHHHHCCCCCCCEEEEECCCCC
YGEKGCAFLILRPGHVPPTIPELIDFLGTQGLAKFKCPERVEVVDEFPVTRVGKVDKPAM
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHHCCCCCCCCCCCHHHH
RRAIAAILETEQLSHTPRQEQ
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]