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The map label for this gene is hprA [H]

Identifier: 78066321

GI number: 78066321

Start: 1868741

End: 1869706

Strand: Reverse

Name: hprA [H]

Synonym: Bcep18194_A4851

Alternate gene names: 78066321

Gene position: 1869706-1868741 (Counterclockwise)

Preceding gene: 78066330

Following gene: 78066309

Centisome position: 50.61

GC content: 70.08

Gene sequence:

>966_bases
ATGTTTTCTGTTTCCTCTCCCGCGAAGATCGTGTTTCTCGACCGCGCGACGCTGTCGCCGCACACCGTGCTGAAGCCGTT
TCCGTTCCCGCACGTGTTGCACACGTTCGACCGGACGGCCGCGCACGAGATGGCCGCGCGAATCGGCGACGCGGACATCG
TCGTGACCAACAAGGTGCGGCTCGACGCGGCGGCGCTCGCCGATGCGCGACGCGTGCGGATGATCGCGATCGCCGCGACG
GGCACCGACATCGTCGATCTCGATGCGTGCGCGGCGCGCGGCATCGTCGTGAGCAATATTCGCGGGTATGCGGCCCGCAC
GGTGCCCGAGCACACGTTCGCGCTGATCTTCGCGCTGCGCCGCAGCCTCGTCGCGTACCGCGACGCGGTGCGTGCGGGGC
GCTGGCTCGCCAGCGGGCAGTTCTGCTTCTTCGATTATTCGATTCGCGACCTGGCCGGCGCGACGCTCGGGATCGTCGGC
GACGGCGTGCTCGGGCGCGCGGTGGCCGCCATCGCGCGTGCGCTCGGCATGCAGGTGCGGTTCGCGGCGCACCGCGATGC
GTCGGGGGACGACTACGTGCCGCTCGATACGCTGCTGCGCGACAGCGACGTGATTACGCTGCACTGCCCGCTGACGCCCG
CGACGCGGCACCTGATCGACGCGGCCGCGTTTGCGCGGATGGCGCGCCGGCCGTTGCTGATCAACACCGCGCGCGGCGGG
CTCGTGGACGAAAGGGCGCTCGTCGACGCATTGCAGTCCGGGCAGATCGCGGGCGCGGGGTTCGATGTGGTCACGCAGGA
GCCGCTGCCGGCCGCGCATCCGTTCCACGCGATCCTGTCGCATCCGGCATTCATCCTGACGCCGCACGTTGCGTGGGCGA
GCGACGAAGCGATGCAGGCGCTGGCCGATCAGCTCGTGGACAATGTCGCCGCGTTCGTGGGCGGCACGCCGCGGCACGTG
GTGTGA

Upstream 100 bases:

>100_bases
GGGAAAGCGCGCTCGCGCAACGGTCAAACGACGGCCCGCGCGCCGCCGGCAGAATCGAACCCGTTCCGCGCCCGCCGGGC
GCCTGTCAGCGAGACCCGCC

Downstream 100 bases:

>100_bases
CGTGTGCGGCGCCTGGCGTTGCGGCGTTGATTTGAATCGCGCAAACAGAACGGCCCCGCTCGATGGCTCGAAGCGGGGCC
GGCTGAATTCGGGGGCTGCT

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MFSVSSPAKIVFLDRATLSPHTVLKPFPFPHVLHTFDRTAAHEMAARIGDADIVVTNKVRLDAAALADARRVRMIAIAAT
GTDIVDLDACAARGIVVSNIRGYAARTVPEHTFALIFALRRSLVAYRDAVRAGRWLASGQFCFFDYSIRDLAGATLGIVG
DGVLGRAVAAIARALGMQVRFAAHRDASGDDYVPLDTLLRDSDVITLHCPLTPATRHLIDAAAFARMARRPLLINTARGG
LVDERALVDALQSGQIAGAGFDVVTQEPLPAAHPFHAILSHPAFILTPHVAWASDEAMQALADQLVDNVAAFVGGTPRHV
V

Sequences:

>Translated_321_residues
MFSVSSPAKIVFLDRATLSPHTVLKPFPFPHVLHTFDRTAAHEMAARIGDADIVVTNKVRLDAAALADARRVRMIAIAAT
GTDIVDLDACAARGIVVSNIRGYAARTVPEHTFALIFALRRSLVAYRDAVRAGRWLASGQFCFFDYSIRDLAGATLGIVG
DGVLGRAVAAIARALGMQVRFAAHRDASGDDYVPLDTLLRDSDVITLHCPLTPATRHLIDAAAFARMARRPLLINTARGG
LVDERALVDALQSGQIAGAGFDVVTQEPLPAAHPFHAILSHPAFILTPHVAWASDEAMQALADQLVDNVAAFVGGTPRHV
V
>Mature_321_residues
MFSVSSPAKIVFLDRATLSPHTVLKPFPFPHVLHTFDRTAAHEMAARIGDADIVVTNKVRLDAAALADARRVRMIAIAAT
GTDIVDLDACAARGIVVSNIRGYAARTVPEHTFALIFALRRSLVAYRDAVRAGRWLASGQFCFFDYSIRDLAGATLGIVG
DGVLGRAVAAIARALGMQVRFAAHRDASGDDYVPLDTLLRDSDVITLHCPLTPATRHLIDAAAFARMARRPLLINTARGG
LVDERALVDALQSGQIAGAGFDVVTQEPLPAAHPFHAILSHPAFILTPHVAWASDEAMQALADQLVDNVAAFVGGTPRHV
V

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=282, Percent_Identity=32.6241134751773, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI145580578, Length=253, Percent_Identity=34.3873517786561, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI4557499, Length=253, Percent_Identity=34.3873517786561, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI61743967, Length=253, Percent_Identity=33.201581027668, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4557497, Length=253, Percent_Identity=33.201581027668, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI145580575, Length=246, Percent_Identity=34.9593495934959, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI6912396, Length=253, Percent_Identity=29.2490118577075, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI87082289, Length=299, Percent_Identity=33.4448160535117, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787645, Length=267, Percent_Identity=30.3370786516854, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1789279, Length=269, Percent_Identity=31.9702602230483, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081824, Length=188, Percent_Identity=28.7234042553192, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788660, Length=277, Percent_Identity=28.5198555956679, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=271, Percent_Identity=30.9963099630996, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=254, Percent_Identity=27.9527559055118, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=325, Percent_Identity=28.9230769230769, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=291, Percent_Identity=30.2405498281787, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324055, Length=270, Percent_Identity=30.7407407407407, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6324964, Length=287, Percent_Identity=28.2229965156794, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324980, Length=117, Percent_Identity=32.4786324786325, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19921140, Length=286, Percent_Identity=31.8181818181818, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI62472511, Length=274, Percent_Identity=33.5766423357664, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24646446, Length=273, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24646448, Length=273, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24646452, Length=273, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24646450, Length=273, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28571528, Length=266, Percent_Identity=31.203007518797, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585516, Length=264, Percent_Identity=28.7878787878788, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574286, Length=257, Percent_Identity=27.6264591439689, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585514, Length=268, Percent_Identity=27.2388059701493, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI28574282, Length=268, Percent_Identity=27.2388059701493, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI28574284, Length=268, Percent_Identity=27.2388059701493, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45551003, Length=268, Percent_Identity=27.2388059701493, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45552429, Length=268, Percent_Identity=27.2388059701493, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34315; Mature: 34315

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSVSSPAKIVFLDRATLSPHTVLKPFPFPHVLHTFDRTAAHEMAARIGDADIVVTNKVR
CCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEE
LDAAALADARRVRMIAIAATGTDIVDLDACAARGIVVSNIRGYAARTVPEHTFALIFALR
EHHHHHHHCCEEEEEEEEECCCCEEECHHHHHCCEEEECCCCHHHHCCCHHHHHHHHHHH
RSLVAYRDAVRAGRWLASGQFCFFDYSIRDLAGATLGIVGDGVLGRAVAAIARALGMQVR
HHHHHHHHHHHHCCEEECCCEEEEECCHHHHCCCEEEEECCCHHHHHHHHHHHHHCCEEE
FAAHRDASGDDYVPLDTLLRDSDVITLHCPLTPATRHLIDAAAFARMARRPLLINTARGG
EEECCCCCCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LVDERALVDALQSGQIAGAGFDVVTQEPLPAAHPFHAILSHPAFILTPHVAWASDEAMQA
CCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHH
LADQLVDNVAAFVGGTPRHVV
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFSVSSPAKIVFLDRATLSPHTVLKPFPFPHVLHTFDRTAAHEMAARIGDADIVVTNKVR
CCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCEE
LDAAALADARRVRMIAIAATGTDIVDLDACAARGIVVSNIRGYAARTVPEHTFALIFALR
EHHHHHHHCCEEEEEEEEECCCCEEECHHHHHCCEEEECCCCHHHHCCCHHHHHHHHHHH
RSLVAYRDAVRAGRWLASGQFCFFDYSIRDLAGATLGIVGDGVLGRAVAAIARALGMQVR
HHHHHHHHHHHHCCEEECCCEEEEECCHHHHCCCEEEEECCCHHHHHHHHHHHHHCCEEE
FAAHRDASGDDYVPLDTLLRDSDVITLHCPLTPATRHLIDAAAFARMARRPLLINTARGG
EEECCCCCCCCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LVDERALVDALQSGQIAGAGFDVVTQEPLPAAHPFHAILSHPAFILTPHVAWASDEAMQA
CCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHCCCEEEEECCCCCCCHHHHHH
LADQLVDNVAAFVGGTPRHVV
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]