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The map label for this gene is sbp [H]

Identifier: 78066209

GI number: 78066209

Start: 1729710

End: 1730747

Strand: Direct

Name: sbp [H]

Synonym: Bcep18194_A4739

Alternate gene names: 78066209

Gene position: 1729710-1730747 (Clockwise)

Preceding gene: 78066205

Following gene: 78066210

Centisome position: 46.82

GC content: 65.32

Gene sequence:

>1038_bases
ATGGGCAAGCGCAACACGGGGCTGGTGGGCGGAGTGGGCCGCCTGATCGCAACACTCGCGCTGGGCGCGGCGGCGGCGCT
GGGCGTCGCGACGCACGCGCAGGCCGATACGACCTTCCTGAACGTGTCGTACGACCCGACGCGCGAGCTGTATCAGGACT
TCAACCAGGCGTTTGGCAAGGAGTGGAAGGCGAAGACGGGCGAGACCGTCAACTTCAAGCAGTCGCACGGCGGTTCGGGC
GCGCAGGCGCGCTCGGTGCTCGACGGCCTGCAGGCCGACGTCGTCACGCTGGCGCTGGCGTACGACATCGACGCGCTCGC
GAACAAGGGGCTCGTCAACAAGGACTGGCAGAAGCGCCTGCCCGACAACGCGTCGCCGTACACGTCGACGATCGTGTTCC
TGGTGCGCAAGGGCAATCCGAAGGGGATCAAGGACTGGGACGACCTGACCAAGCCGGGCATCTCGATCGTCACGCCGAAT
CCGAAGACGTCGGGCGGCGCGCGCTGGAACTACCTGGCCGCATGGGCATACGCGGTGCACAAGCCGGGCGGCAACGAGCA
GACGGCGAAGGAATTCGTCACGAAGCTCTACAAGAACGCAGGCGTGCTCGATTCGGGCGCGCGCGGCGCGACCACGAGCT
TCGTGCAGCGCGGGATTGGCGACGTGCTGATCGCCTGGGAAAACGAGGCATTCCTGTCGGTCAAGGAATTCGGTACCGAC
AAGTTCGAGATCGTCGTGCCGTCGGTGAGCATCCTGGCCGAGCCGCCCGTCGCGGTGGTGGACAAGGTGGTCGACAAGAA
GGGTACGCGCAAGCTGGCCGATGCCTACCTGAACTTCCTGTACAGCCCGCAGGGCCAGGAGATCGCGGCGCGCAACTACT
ACCGGCCGCGCTCGAAAAACGTGCCGGCGGAGCTGACGAAGCAGTTCCCGAAGCTGAAGCTGTACACGGTCGACGATACC
TTCGGCGGCTGGACGAATGCGCAGAAGACGCATTTCGCGGACGGCGGCGTGTTCGATTCGATCTACAAGCCGCAGTAA

Upstream 100 bases:

>100_bases
TTTTGAGTATTAAAAAATGAGGAACGATTATTTTTAATCATGTAACCCGTTCGTTAGACTGGCCCGACATCGCAATACCG
ACAACACTGGAGAACCAAGG

Downstream 100 bases:

>100_bases
CGCCATAACGACAGCGGCGCGCCACGACAGCGCGCCGCATCCCCGACGGCCCGCCGGCGCGATTCACTGCGCCGGCGTTT
GCGTCGGACCCATGAGCAGC

Product: thiosulphate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MGKRNTGLVGGVGRLIATLALGAAAALGVATHAQADTTFLNVSYDPTRELYQDFNQAFGKEWKAKTGETVNFKQSHGGSG
AQARSVLDGLQADVVTLALAYDIDALANKGLVNKDWQKRLPDNASPYTSTIVFLVRKGNPKGIKDWDDLTKPGISIVTPN
PKTSGGARWNYLAAWAYAVHKPGGNEQTAKEFVTKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSVKEFGTD
KFEIVVPSVSILAEPPVAVVDKVVDKKGTRKLADAYLNFLYSPQGQEIAARNYYRPRSKNVPAELTKQFPKLKLYTVDDT
FGGWTNAQKTHFADGGVFDSIYKPQ

Sequences:

>Translated_345_residues
MGKRNTGLVGGVGRLIATLALGAAAALGVATHAQADTTFLNVSYDPTRELYQDFNQAFGKEWKAKTGETVNFKQSHGGSG
AQARSVLDGLQADVVTLALAYDIDALANKGLVNKDWQKRLPDNASPYTSTIVFLVRKGNPKGIKDWDDLTKPGISIVTPN
PKTSGGARWNYLAAWAYAVHKPGGNEQTAKEFVTKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSVKEFGTD
KFEIVVPSVSILAEPPVAVVDKVVDKKGTRKLADAYLNFLYSPQGQEIAARNYYRPRSKNVPAELTKQFPKLKLYTVDDT
FGGWTNAQKTHFADGGVFDSIYKPQ
>Mature_344_residues
GKRNTGLVGGVGRLIATLALGAAAALGVATHAQADTTFLNVSYDPTRELYQDFNQAFGKEWKAKTGETVNFKQSHGGSGA
QARSVLDGLQADVVTLALAYDIDALANKGLVNKDWQKRLPDNASPYTSTIVFLVRKGNPKGIKDWDDLTKPGISIVTPNP
KTSGGARWNYLAAWAYAVHKPGGNEQTAKEFVTKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSVKEFGTDK
FEIVVPSVSILAEPPVAVVDKVVDKKGTRKLADAYLNFLYSPQGQEIAARNYYRPRSKNVPAELTKQFPKLKLYTVDDTF
GGWTNAQKTHFADGGVFDSIYKPQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=323, Percent_Identity=64.7058823529412, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1788765, Length=312, Percent_Identity=49.0384615384615, Blast_Score=300, Evalue=8e-83,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37424; Mature: 37292

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRNTGLVGGVGRLIATLALGAAAALGVATHAQADTTFLNVSYDPTRELYQDFNQAFGK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCC
EWKAKTGETVNFKQSHGGSGAQARSVLDGLQADVVTLALAYDIDALANKGLVNKDWQKRL
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
PDNASPYTSTIVFLVRKGNPKGIKDWDDLTKPGISIVTPNPKTSGGARWNYLAAWAYAVH
CCCCCCCCEEEEEEEECCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHC
KPGGNEQTAKEFVTKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSVKEFGTD
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEHHHCCCC
KFEIVVPSVSILAEPPVAVVDKVVDKKGTRKLADAYLNFLYSPQGQEIAARNYYRPRSKN
EEEEEECCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
VPAELTKQFPKLKLYTVDDTFGGWTNAQKTHFADGGVFDSIYKPQ
CCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKRNTGLVGGVGRLIATLALGAAAALGVATHAQADTTFLNVSYDPTRELYQDFNQAFGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCC
EWKAKTGETVNFKQSHGGSGAQARSVLDGLQADVVTLALAYDIDALANKGLVNKDWQKRL
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHC
PDNASPYTSTIVFLVRKGNPKGIKDWDDLTKPGISIVTPNPKTSGGARWNYLAAWAYAVH
CCCCCCCCEEEEEEEECCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHC
KPGGNEQTAKEFVTKLYKNAGVLDSGARGATTSFVQRGIGDVLIAWENEAFLSVKEFGTD
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEHHHCCCC
KFEIVVPSVSILAEPPVAVVDKVVDKKGTRKLADAYLNFLYSPQGQEIAARNYYRPRSKN
EEEEEECCHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCC
VPAELTKQFPKLKLYTVDDTFGGWTNAQKTHFADGGVFDSIYKPQ
CCHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]