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The map label for this gene is iolD [H]

Identifier: 78066037

GI number: 78066037

Start: 1538048

End: 1539958

Strand: Reverse

Name: iolD [H]

Synonym: Bcep18194_A4566

Alternate gene names: 78066037

Gene position: 1539958-1538048 (Counterclockwise)

Preceding gene: 78066038

Following gene: 78066036

Centisome position: 41.69

GC content: 69.18

Gene sequence:

>1911_bases
ATGACCCATTCCTCTTCACCGACCGACATGACCTCTACCGTGAGACTGACCGTCAGCCAGGCGCTCGTGCGCTACCTGGC
CGCCCTGCGCGCCGAAGTCGTCCAGCCCGACGGCCGCACCGAGATCCTGCCGTACTGCGGCGGCGTGTTCGCGATCTTCG
GCCACGGCAACGTGGCCGGGCTCGGCGAGGCGCTGCACGCGGAAAAAGACCGCCTGCCGACCTTCCGCGCGCACAACGAG
CAAGGGATGGCCAATGCGGCCGTCGCGTTCGCGAAAGCGAATTTCCGCCAGCGGATGATGGCCGCGACGTCGAGCATCGG
CCCCGGCGCGACCAACATGCTGACGTCGGCCGCGATCGCGCACGTCGGCCGGCTGCCGTTGTTGCTGCTGCCCGGCGACG
TGTTCGTGTCGCGGCTGCCCGACCCCGTGCTGCAGCAGGTCGAGGATTTCGAACAGGGCGACGTCAGCGCGAACGACTGC
TTCCGGCCGGTGACACGCTACTTCGACCGGATCACGTCGCCCGAGCAGTTGCTCGTCGCGCTGCCGCGTGCGATCCAGGT
GATGACCGACCCCGCGCAATGCGGCCCCGTGTGCCTCGCGCTGCCGCAGGACGTGCAGACCTTCGCGTTCGACTGGCCCG
AGGATTTCTTCGCGCCGCCGGTGATCCGGATGCGCCGGCCGCCGGCCGACGCGCTCGAACTCGCCGATGCGCTCGACATG
CTGAAGGCCGCGAAGAAACCGCTGATCGTCGCGGGCGGCGGCGTGCTGTACAGCCAGGCATGGGACGCGCTGCGTGCGTT
CGCCGATACGCATGGCGTGCCGGTCGCCGAATCGCAGGCCGGCAAGGGCAGCCTCGCGTGGGACCATCCGCTGAATCTCG
GGTCGATCGGCGTGACGGGCTCGCCTGCCGCGAACCGTGCGGCCGCGCAGGCCGACGTGGTGTTCGCAGTCGGCACGCGG
CTGCAGGATTTCACGACCGGCTCGCATGCGCTCTACGGCAACGCGACGCTGTTGAGCCTGAACGTGCAGCCGTTCGACGC
GGGCAAGAAACGCGGCCGTCAACTGGTGGCCGATGCGCGCACGGGCCTCGGCCAGCTGTCGGCCGCACTGGCCGGCTGGC
AGGCCGACCCGGCATGGACCGCAAGCAACCGCGACCAGGCCGCCGCATGGAATGCGCGCGTGACCGAACTCACGACGCGC
GTGCCGAAAGACACGCTGCCGTACGACGCGGAAGTGATCGGCGCGGTGCGCGATTCCGCAACCGACGCGGGACTCGACAG
CGCGCGCGACGACCTCGTCGTATGCGCGGCCGGTACGCTGCCGGCCGAGCTGCACAAGCTGTGGCGCAGCGGCGTGCCGG
GCAACTACCACATGGACTATGCGTACTCGTGCATGGGCTACGAAGTCGCGGGCGGCCTCGGCGCGAAGCTTGCGCGGCCC
GAGCGCGAAGTGATCGTGATCGTCGGCGACGGCTCGTACATGATGCTCAACGCGGAACTCGCGACGTCGGTGATGCTCGG
CCGCAAGATCATCGTCGTGATCCTCGACAACCGCGGCTACGGCTGCATCGAGCGGCTGCAACTGAATTGCGGTGGCGCGA
GCTTCAACAACATGCTCGACGACTGCGTGCCGGAGGGCGGCGAGCGCTCGACGATCGATTTCGCGATGCATGCGCGTGCG
ATGGGCGCCGAAGCCGTGCACGTGCGCGACATCGGCGAGCTGCGCAGCGAGATGAAGCGCGCCCGCGCGGCAAAGACGAG
CCAGGTGCTCGTGATCGACACCACGCACCAGCGCACGACCGACGACGGCGGCGCGTGGTGGGAGGTCGCGGTGCCGCAAG
TATCCGAACGCGCCGGCGTCGACGCAGCACACCGCGCGTATCTCGATGCGAAGACCCGGCAGCGGCGTTGA

Upstream 100 bases:

>100_bases
ATCGACGAAGTCGCCGGCAACTTCGCGCAACTGGTCGACGCGTGGCTCGGCCGCCGTGCCGCGGCCGCCCGCGCCGCCGC
GGCCTGACGCGACGCCTGCG

Downstream 100 bases:

>100_bases
TCGCGCGCACTTGCCCACGAACCCTCCCCGCAAAAAACATAGTCGAACCAAGGAACCCCGTCATGAGCTGGAACGTCCGC
ATCGGTATCAATCCCCTGTC

Product: acetolactate synthase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MTHSSSPTDMTSTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAGLGEALHAEKDRLPTFRAHNE
QGMANAAVAFAKANFRQRMMAATSSIGPGATNMLTSAAIAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDC
FRPVTRYFDRITSPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAFDWPEDFFAPPVIRMRRPPADALELADALDM
LKAAKKPLIVAGGGVLYSQAWDALRAFADTHGVPVAESQAGKGSLAWDHPLNLGSIGVTGSPAANRAAAQADVVFAVGTR
LQDFTTGSHALYGNATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAALAGWQADPAWTASNRDQAAAWNARVTELTTR
VPKDTLPYDAEVIGAVRDSATDAGLDSARDDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARP
EREVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGERSTIDFAMHARA
MGAEAVHVRDIGELRSEMKRARAAKTSQVLVIDTTHQRTTDDGGAWWEVAVPQVSERAGVDAAHRAYLDAKTRQRR

Sequences:

>Translated_636_residues
MTHSSSPTDMTSTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAGLGEALHAEKDRLPTFRAHNE
QGMANAAVAFAKANFRQRMMAATSSIGPGATNMLTSAAIAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDC
FRPVTRYFDRITSPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAFDWPEDFFAPPVIRMRRPPADALELADALDM
LKAAKKPLIVAGGGVLYSQAWDALRAFADTHGVPVAESQAGKGSLAWDHPLNLGSIGVTGSPAANRAAAQADVVFAVGTR
LQDFTTGSHALYGNATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAALAGWQADPAWTASNRDQAAAWNARVTELTTR
VPKDTLPYDAEVIGAVRDSATDAGLDSARDDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARP
EREVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGERSTIDFAMHARA
MGAEAVHVRDIGELRSEMKRARAAKTSQVLVIDTTHQRTTDDGGAWWEVAVPQVSERAGVDAAHRAYLDAKTRQRR
>Mature_635_residues
THSSSPTDMTSTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAGLGEALHAEKDRLPTFRAHNEQ
GMANAAVAFAKANFRQRMMAATSSIGPGATNMLTSAAIAHVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDCF
RPVTRYFDRITSPEQLLVALPRAIQVMTDPAQCGPVCLALPQDVQTFAFDWPEDFFAPPVIRMRRPPADALELADALDML
KAAKKPLIVAGGGVLYSQAWDALRAFADTHGVPVAESQAGKGSLAWDHPLNLGSIGVTGSPAANRAAAQADVVFAVGTRL
QDFTTGSHALYGNATLLSLNVQPFDAGKKRGRQLVADARTGLGQLSAALAGWQADPAWTASNRDQAAAWNARVTELTTRV
PKDTLPYDAEVIGAVRDSATDAGLDSARDDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARPE
REVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLDDCVPEGGERSTIDFAMHARAM
GAEAVHVRDIGELRSEMKRARAAKTSQVLVIDTTHQRTTDDGGAWWEVAVPQVSERAGVDAAHRAYLDAKTRQRR

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=28.2685512367491, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1786717, Length=514, Percent_Identity=25.6809338521401, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790104, Length=484, Percent_Identity=25.2066115702479, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788716, Length=480, Percent_Identity=23.5416666666667, Blast_Score=76, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=607, Percent_Identity=25.2059308072488, Blast_Score=146, Evalue=9e-36,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 68122; Mature: 67991

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSSSPTDMTSTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCHHH
LGEALHAEKDRLPTFRAHNEQGMANAAVAFAKANFRQRMMAATSSIGPGATNMLTSAAIA
HHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
HVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDCFRPVTRYFDRITSPEQLLVA
HHCCCCEEEECHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LPRAIQVMTDPAQCGPVCLALPQDVQTFAFDWPEDFFAPPVIRMRRPPADALELADALDM
HHHHHHEECCCHHCCCEEEECCCCHHHHCCCCCHHHCCCCHHEECCCCHHHHHHHHHHHH
LKAAKKPLIVAGGGVLYSQAWDALRAFADTHGVPVAESQAGKGSLAWDHPLNLGSIGVTG
HHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPAANRAAAQADVVFAVGTRLQDFTTGSHALYGNATLLSLNVQPFDAGKKRGRQLVADAR
CCCCCCHHHHHCEEEEECCCHHHCCCCCCEEECCEEEEEEECCCCCCHHHHCHHHHHHHH
TGLGQLSAALAGWQADPAWTASNRDQAAAWNARVTELTTRVPKDTLPYDAEVIGAVRDSA
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCC
TDAGLDSARDDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARP
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECEEEEECCEEECCCCCCCCCCC
EREVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLD
CCEEEEEEECCCEEEEEHHHHHHHHHCCEEEEEEECCCCCCCEEHEEECCCCCCHHHHHH
DCVPEGGERSTIDFAMHARAMGAEAVHVRDIGELRSEMKRARAAKTSQVLVIDTTHQRTT
HHCCCCCCCCEEHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
DDGGAWWEVAVPQVSERAGVDAAHRAYLDAKTRQRR
CCCCCEEEEECCCHHHHCCCCHHHHHHHCCHHHCCC
>Mature Secondary Structure 
THSSSPTDMTSTVRLTVSQALVRYLAALRAEVVQPDGRTEILPYCGGVFAIFGHGNVAG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEECCCHHH
LGEALHAEKDRLPTFRAHNEQGMANAAVAFAKANFRQRMMAATSSIGPGATNMLTSAAIA
HHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
HVGRLPLLLLPGDVFVSRLPDPVLQQVEDFEQGDVSANDCFRPVTRYFDRITSPEQLLVA
HHCCCCEEEECHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
LPRAIQVMTDPAQCGPVCLALPQDVQTFAFDWPEDFFAPPVIRMRRPPADALELADALDM
HHHHHHEECCCHHCCCEEEECCCCHHHHCCCCCHHHCCCCHHEECCCCHHHHHHHHHHHH
LKAAKKPLIVAGGGVLYSQAWDALRAFADTHGVPVAESQAGKGSLAWDHPLNLGSIGVTG
HHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPAANRAAAQADVVFAVGTRLQDFTTGSHALYGNATLLSLNVQPFDAGKKRGRQLVADAR
CCCCCCHHHHHCEEEEECCCHHHCCCCCCEEECCEEEEEEECCCCCCHHHHCHHHHHHHH
TGLGQLSAALAGWQADPAWTASNRDQAAAWNARVTELTTRVPKDTLPYDAEVIGAVRDSA
CCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCC
TDAGLDSARDDLVVCAAGTLPAELHKLWRSGVPGNYHMDYAYSCMGYEVAGGLGAKLARP
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECEEEEECCEEECCCCCCCCCCC
EREVIVIVGDGSYMMLNAELATSVMLGRKIIVVILDNRGYGCIERLQLNCGGASFNNMLD
CCEEEEEEECCCEEEEEHHHHHHHHHCCEEEEEEECCCCCCCEEHEEECCCCCCHHHHHH
DCVPEGGERSTIDFAMHARAMGAEAVHVRDIGELRSEMKRARAAKTSQVLVIDTTHQRTT
HHCCCCCCCCEEHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
DDGGAWWEVAVPQVSERAGVDAAHRAYLDAKTRQRR
CCCCCEEEEECCCHHHHCCCCHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA