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The map label for this gene is yneJ [H]

Identifier: 78066025

GI number: 78066025

Start: 1527491

End: 1528408

Strand: Reverse

Name: yneJ [H]

Synonym: Bcep18194_A4554

Alternate gene names: 78066025

Gene position: 1528408-1527491 (Counterclockwise)

Preceding gene: 78066034

Following gene: 78066023

Centisome position: 41.37

GC content: 72.11

Gene sequence:

>918_bases
ATGGACCTGGCGGCACTGGCGATTTTCCGGGCCGTGGTGCGCGAGAACGGCGTGACGCGCGCGGCGGCCAAGCTCAATCG
CGTGCAGTCGAACGTCACGACGCGCATCAAGCAGCTCGAGGAAGAGCTCGGTGCGGCGCTGTTCGTGCGCGACGGCCGCC
GGCTCGTGCTGACGCCGGCCGGGCACACGCTGCTGCCGTATGCGGAGCGCCTGCTCGCGCTCGCCGACGAGGCACGCGAC
GCGGTACGCGAGGACACGCCGCGCGGTCGGCTGCGGCTCGGCACGATGGAGAGCACGGCTGCGAGCCGCCTGCCGACCGT
GCTCGCGCGCTATCACGACGCGTGGCCCGACGTGTCGCTCGAACTGCTGACGGGCACGACGGGCTGGCTGATCGACCGCG
TGCGCGACTTCGAGATCGACGCCGCGCTGTTCGCGCGCCCGCCCGCGCCGGACACGCTGCCCGACACGTTCGAGACGGTA
CCGGTCTTCCGCGAAAACCTGGTGCTGCTCACCCCACGCGGCCACCCGCCGGTGCGCACGCCGCGCGACGTGATCCTGCC
GACGCTGATCGCATTCGAGCGCGGCTGCACGTATCGCAAGTACGTCGAGCAGTGGTACGCGGCGCATGGCGTCAAGCCGG
CGCGCGTGCTCGAACTCGGGTCGTATCACGCGATCGTCGCGTGCGTCGCGGCGGGTGCCGGCATCGCGGTCGCGCCGCGT
TCGGTGCTCGACCTGCAGCCCGAGACCGGCAACATCACCGCGCACGTGATCCCCGAACTGGAAGGCATCGATACGCTGCT
CGCGTGGCGGCAGGGCTATGCATCGGCGGCGCTGGCCGCGTTACGCGATGCGTTGAGCGACGCGGCCGCACGGCAGCCGG
ACGGTGCGGCGCAGCCGCCGGCCACCGTGCCGGCCTGA

Upstream 100 bases:

>100_bases
GCGCCGGCCGGATCGGAAACCGGAATGCTTCCATCCTAGCTTGACCAAACGTTCTTGAGAAATGAATAATTAAGAATCCA
CCCATCGCTCGGAGAGAATC

Downstream 100 bases:

>100_bases
ATCGTCGGGCCGCCGGCATTCATTGTTCCGGCGTACCAGCAAAAAGACCCGACCGGCATGTGCCGATCGGGTCTTTTTCA
TTTCACGCGATGCCGTAACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPAGHTLLPYAERLLALADEARD
AVREDTPRGRLRLGTMESTAASRLPTVLARYHDAWPDVSLELLTGTTGWLIDRVRDFEIDAALFARPPAPDTLPDTFETV
PVFRENLVLLTPRGHPPVRTPRDVILPTLIAFERGCTYRKYVEQWYAAHGVKPARVLELGSYHAIVACVAAGAGIAVAPR
SVLDLQPETGNITAHVIPELEGIDTLLAWRQGYASAALAALRDALSDAAARQPDGAAQPPATVPA

Sequences:

>Translated_305_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPAGHTLLPYAERLLALADEARD
AVREDTPRGRLRLGTMESTAASRLPTVLARYHDAWPDVSLELLTGTTGWLIDRVRDFEIDAALFARPPAPDTLPDTFETV
PVFRENLVLLTPRGHPPVRTPRDVILPTLIAFERGCTYRKYVEQWYAAHGVKPARVLELGSYHAIVACVAAGAGIAVAPR
SVLDLQPETGNITAHVIPELEGIDTLLAWRQGYASAALAALRDALSDAAARQPDGAAQPPATVPA
>Mature_305_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPAGHTLLPYAERLLALADEARD
AVREDTPRGRLRLGTMESTAASRLPTVLARYHDAWPDVSLELLTGTTGWLIDRVRDFEIDAALFARPPAPDTLPDTFETV
PVFRENLVLLTPRGHPPVRTPRDVILPTLIAFERGCTYRKYVEQWYAAHGVKPARVLELGSYHAIVACVAAGAGIAVAPR
SVLDLQPETGNITAHVIPELEGIDTLLAWRQGYASAALAALRDALSDAAARQPDGAAQPPATVPA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=299, Percent_Identity=41.8060200668896, Blast_Score=227, Evalue=8e-61,
Organism=Escherichia coli, GI87082132, Length=286, Percent_Identity=29.7202797202797, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=31.0344827586207, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=30.078125, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788296, Length=246, Percent_Identity=31.7073170731707, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788887, Length=290, Percent_Identity=27.9310344827586, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI157672245, Length=229, Percent_Identity=27.0742358078603, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=25, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=26.7441860465116, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787879, Length=256, Percent_Identity=27.34375, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788748, Length=292, Percent_Identity=25.6849315068493, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789204, Length=256, Percent_Identity=28.125, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=27.2357723577236, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788626, Length=149, Percent_Identity=30.8724832214765, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790262, Length=302, Percent_Identity=25.8278145695364, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787530, Length=267, Percent_Identity=25.4681647940075, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994962, Length=154, Percent_Identity=31.8181818181818, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786401, Length=151, Percent_Identity=32.4503311258278, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=264, Percent_Identity=26.8939393939394, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33077; Mature: 33077

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCC
GHTLLPYAERLLALADEARDAVREDTPRGRLRLGTMESTAASRLPTVLARYHDAWPDVSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEE
ELLTGTTGWLIDRVRDFEIDAALFARPPAPDTLPDTFETVPVFRENLVLLTPRGHPPVRT
EEECCCHHHHHHHHHCCCCCHHHEECCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCCC
PRDVILPTLIAFERGCTYRKYVEQWYAAHGVKPARVLELGSYHAIVACVAAGAGIAVAPR
CHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCEECCH
SVLDLQPETGNITAHVIPELEGIDTLLAWRQGYASAALAALRDALSDAAARQPDGAAQPP
HHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ATVPA
CCCCC
>Mature Secondary Structure
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCC
GHTLLPYAERLLALADEARDAVREDTPRGRLRLGTMESTAASRLPTVLARYHDAWPDVSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEE
ELLTGTTGWLIDRVRDFEIDAALFARPPAPDTLPDTFETVPVFRENLVLLTPRGHPPVRT
EEECCCHHHHHHHHHCCCCCHHHEECCCCCCCCCCCHHHHHHHHCCEEEECCCCCCCCCC
PRDVILPTLIAFERGCTYRKYVEQWYAAHGVKPARVLELGSYHAIVACVAAGAGIAVAPR
CHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCEECCH
SVLDLQPETGNITAHVIPELEGIDTLLAWRQGYASAALAALRDALSDAAARQPDGAAQPP
HHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ATVPA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]