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The map label for this gene is nfdA [H]

Identifier: 78066010

GI number: 78066010

Start: 1509824

End: 1511713

Strand: Direct

Name: nfdA [H]

Synonym: Bcep18194_A4539

Alternate gene names: 78066010

Gene position: 1509824-1511713 (Clockwise)

Preceding gene: 78066009

Following gene: 78066011

Centisome position: 40.87

GC content: 66.61

Gene sequence:

>1890_bases
ATGAGCGCAACCGCAACTCAACCCGATCTCATCCTGCACAACGGGCGCATCACGACGCTCGATCATGCGAACCCCGTCGC
TACCGCGGTGGCAATCAAGGACGGCCGCTTCGTCGCGGTCGGCGCCGACGCCGATATCGTGCCGCTCGCGGGCCGCGCGA
CGAAGGTCGTCGATCTCGATTGCCGCAGCGTGCTGCCGGGCCTGATCGACAACCACACGCACGTGATTCGCGGCGGCCTG
AACTACAACCTCGAGCTGCGCTGGGATGGCGTGCGTTCGCTCGCGGTCGCGATGGAGATGCTGAAGCACCAGGTCGCGAT
CACGCCTGCGCCGCAATGGGTGCGCGTGGTTGGCGGCTTCACCGAACACCAGTTCGCGGAGAAGCGCCTGCCGACGATCG
ACGAACTCAATGCCGTCGCACCCGATACGCCGGTGTTCATCCTGCACCTGTACGACCGTGCGCTGCTGAATGCGGCCGCG
CTGCGGGTGGTCGGCTACACGAAGGACACGCCCGAGCCGCCGGGCGGCACGATCCTGCGCGATGAAGCAGGCAACCCGAC
CGGCCTGCTGCTCGCGAACCCGAACGCGACGATCCTCTACGCAACGCTCGCGAAAGGGCCGAAGCTGCCGTTCGACTACC
AGTACAACTCGACGCGCCACTTCATGCGCGAACTGAACCGGCTTGGCGTGACGGGCGTGATCGACGCAGGCGGCGGTTCG
CAGAATTATCCCGACGATTACGAAGTGATCCGCAAGCTGCACGACGCGGGCGAGATGACCGTGCGGATCGCGTACAACCT
GTTCACGCAGAAGCCGAACGCGGAAAAGGAAGACTTCGTGAACTGGACCAAGAGCGTCAAGTACCACGACGGCACCGACT
ACTTCCGCCACAACGGCGCGGGCGAGATGCTGGTGTTCTCGGCGGCCGACTTCGAGGATTTCCGCGTCGCGCGCCCGGAT
CTGCCGGCGCAGATGGAGGACGATCTCGAAGGCGTCGTGCGCGTGCTCGCACAGAACCGCTGGCCGTGGCGCATGCATGC
GACCTACGACGAGACGATCAGCCGCGCGCTCGACGTGTTCGAGAAGGTGAACCGCGATATTCCGCTCGACGGGCTGAACT
GGTTCTTCGATCATGCGGAAACGGTCTCCGATAAATCGATGGACCGGATTGCCGCGCTCGGCGGCGGCATTGCAGTGCAG
CACCGGATGGCGTACCAGGGCGAATACTTCGTCGAGCGCTACGGCGCGCAGGCGGCCGAGGCGACGCCGCCGGTCGCGAA
GATGCTCGACAAGGGGATCAAGGTGTCGGCCGGCACCGACGCGACACGCGTGGCGTCGTACAACCCGTGGGTGTCGCTCG
CGTGGCTCGTGACGGGCAAGACGGTCGGCGGCCTGCGGATGTATCCGCAGCGCAACCTGCTCGATCGCGAGACGGCGCTG
CGCATGTGGACCGAGTACGTGACGTGGTTTTCGAACGAAGAGGGGAAGAAAGGGCGCATCGCGGTCGGCCAGCTCGCCGA
CCTGATGGTCCCGGATCGCGATTTCTTCACGTGTGCGGAGGACGATATCGTCGATACGACTGCATTGCTGACGGTGGTCG
GCGGCCGGATCGTGTGGGGGGCGGGGCCGTTCGCGTCGCACGATGCGCCGATTCCGCCCGCGATGCCTGACTGGTCGCCG
GTGCGCGAGTACGGCGGCTACGGCGGCTGGGGCGCGACGCAGCGCAACGGCGCGCCGCTGCAACGCGCGGCGGCCGCCGC
GATGTGCGGCTGCTCGAGCGCCTGCAACGTCCACGGTCATGCGCATGCCGGCGCATGGGGCCGCGCGTTGCCGACGTCGG
ATGCGAAGGGCTTCTGGGGCGCGTTCGGCTGTTCGTGCTGGGCGGTCTGA

Upstream 100 bases:

>100_bases
GCTGATCGCACTGGTCGGCCTGGCCGGCATGCTGATCGGCGCCCAGGCCATCGCGCCGATCCGTCACTGGCTCGGCTTCT
GACCGCATCGAGGACACTTC

Downstream 100 bases:

>100_bases
CATGGCGACTCCGACCACTCGCGGCGGCGCCGGCAACCCGGCCTGGGTCCGCGCGCTGCTGGCGCAGCCGTGGGTGCTGC
CGCTTGCGCGCCTGGCGCTG

Product: metal-dependent hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSATATQPDLILHNGRITTLDHANPVATAVAIKDGRFVAVGADADIVPLAGRATKVVDLDCRSVLPGLIDNHTHVIRGGL
NYNLELRWDGVRSLAVAMEMLKHQVAITPAPQWVRVVGGFTEHQFAEKRLPTIDELNAVAPDTPVFILHLYDRALLNAAA
LRVVGYTKDTPEPPGGTILRDEAGNPTGLLLANPNATILYATLAKGPKLPFDYQYNSTRHFMRELNRLGVTGVIDAGGGS
QNYPDDYEVIRKLHDAGEMTVRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGAGEMLVFSAADFEDFRVARPD
LPAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVFEKVNRDIPLDGLNWFFDHAETVSDKSMDRIAALGGGIAVQ
HRMAYQGEYFVERYGAQAAEATPPVAKMLDKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETAL
RMWTEYVTWFSNEEGKKGRIAVGQLADLMVPDRDFFTCAEDDIVDTTALLTVVGGRIVWGAGPFASHDAPIPPAMPDWSP
VREYGGYGGWGATQRNGAPLQRAAAAAMCGCSSACNVHGHAHAGAWGRALPTSDAKGFWGAFGCSCWAV

Sequences:

>Translated_629_residues
MSATATQPDLILHNGRITTLDHANPVATAVAIKDGRFVAVGADADIVPLAGRATKVVDLDCRSVLPGLIDNHTHVIRGGL
NYNLELRWDGVRSLAVAMEMLKHQVAITPAPQWVRVVGGFTEHQFAEKRLPTIDELNAVAPDTPVFILHLYDRALLNAAA
LRVVGYTKDTPEPPGGTILRDEAGNPTGLLLANPNATILYATLAKGPKLPFDYQYNSTRHFMRELNRLGVTGVIDAGGGS
QNYPDDYEVIRKLHDAGEMTVRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGAGEMLVFSAADFEDFRVARPD
LPAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVFEKVNRDIPLDGLNWFFDHAETVSDKSMDRIAALGGGIAVQ
HRMAYQGEYFVERYGAQAAEATPPVAKMLDKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETAL
RMWTEYVTWFSNEEGKKGRIAVGQLADLMVPDRDFFTCAEDDIVDTTALLTVVGGRIVWGAGPFASHDAPIPPAMPDWSP
VREYGGYGGWGATQRNGAPLQRAAAAAMCGCSSACNVHGHAHAGAWGRALPTSDAKGFWGAFGCSCWAV
>Mature_628_residues
SATATQPDLILHNGRITTLDHANPVATAVAIKDGRFVAVGADADIVPLAGRATKVVDLDCRSVLPGLIDNHTHVIRGGLN
YNLELRWDGVRSLAVAMEMLKHQVAITPAPQWVRVVGGFTEHQFAEKRLPTIDELNAVAPDTPVFILHLYDRALLNAAAL
RVVGYTKDTPEPPGGTILRDEAGNPTGLLLANPNATILYATLAKGPKLPFDYQYNSTRHFMRELNRLGVTGVIDAGGGSQ
NYPDDYEVIRKLHDAGEMTVRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGAGEMLVFSAADFEDFRVARPDL
PAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVFEKVNRDIPLDGLNWFFDHAETVSDKSMDRIAALGGGIAVQH
RMAYQGEYFVERYGAQAAEATPPVAKMLDKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETALR
MWTEYVTWFSNEEGKKGRIAVGQLADLMVPDRDFFTCAEDDIVDTTALLTVVGGRIVWGAGPFASHDAPIPPAMPDWSPV
REYGGYGGWGATQRNGAPLQRAAAAAMCGCSSACNVHGHAHAGAWGRALPTSDAKGFWGAFGCSCWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 68880; Mature: 68748

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATATQPDLILHNGRITTLDHANPVATAVAIKDGRFVAVGADADIVPLAGRATKVVDLD
CCCCCCCCCEEEECCEEEEECCCCCEEEEEEEECCEEEEECCCCCEEECCCCCEEEEECC
CRSVLPGLIDNHTHVIRGGLNYNLELRWDGVRSLAVAMEMLKHQVAITPAPQWVRVVGGF
HHHHCCHHHCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHHEEECCCCHHHHHHCCC
TEHQFAEKRLPTIDELNAVAPDTPVFILHLYDRALLNAAALRVVGYTKDTPEPPGGTILR
HHHHHHHHCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCEEE
DEAGNPTGLLLANPNATILYATLAKGPKLPFDYQYNSTRHFMRELNRLGVTGVIDAGGGS
CCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCC
QNYPDDYEVIRKLHDAGEMTVRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGA
CCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCEEECCCCHHHHCCCC
GEMLVFSAADFEDFRVARPDLPAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVF
CCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
EKVNRDIPLDGLNWFFDHAETVSDKSMDRIAALGGGIAVQHRMAYQGEYFVERYGAQAAE
HHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCCC
ATPPVAKMLDKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETAL
CCCHHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCHHHHHHH
RMWTEYVTWFSNEEGKKGRIAVGQLADLMVPDRDFFTCAEDDIVDTTALLTVVGGRIVWG
HHHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCEEEEE
AGPFASHDAPIPPAMPDWSPVREYGGYGGWGATQRNGAPLQRAAAAAMCGCSSACNVHGH
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
AHAGAWGRALPTSDAKGFWGAFGCSCWAV
CCCCCCCCCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
SATATQPDLILHNGRITTLDHANPVATAVAIKDGRFVAVGADADIVPLAGRATKVVDLD
CCCCCCCCEEEECCEEEEECCCCCEEEEEEEECCEEEEECCCCCEEECCCCCEEEEECC
CRSVLPGLIDNHTHVIRGGLNYNLELRWDGVRSLAVAMEMLKHQVAITPAPQWVRVVGGF
HHHHCCHHHCCCCEEEECCCCEEEEEEECHHHHHHHHHHHHHHHEEECCCCHHHHHHCCC
TEHQFAEKRLPTIDELNAVAPDTPVFILHLYDRALLNAAALRVVGYTKDTPEPPGGTILR
HHHHHHHHCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCEEE
DEAGNPTGLLLANPNATILYATLAKGPKLPFDYQYNSTRHFMRELNRLGVTGVIDAGGGS
CCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCC
QNYPDDYEVIRKLHDAGEMTVRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRHNGA
CCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCEEECCCCHHHHCCCC
GEMLVFSAADFEDFRVARPDLPAQMEDDLEGVVRVLAQNRWPWRMHATYDETISRALDVF
CCEEEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
EKVNRDIPLDGLNWFFDHAETVSDKSMDRIAALGGGIAVQHRMAYQGEYFVERYGAQAAE
HHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHCCCCCC
ATPPVAKMLDKGIKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETAL
CCCHHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCHHHHHHH
RMWTEYVTWFSNEEGKKGRIAVGQLADLMVPDRDFFTCAEDDIVDTTALLTVVGGRIVWG
HHHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCEEEEE
AGPFASHDAPIPPAMPDWSPVREYGGYGGWGATQRNGAPLQRAAAAAMCGCSSACNVHGH
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
AHAGAWGRALPTSDAKGFWGAFGCSCWAV
CCCCCCCCCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA