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The map label for this gene is mhpA

Identifier: 78065954

GI number: 78065954

Start: 1447913

End: 1449580

Strand: Reverse

Name: mhpA

Synonym: Bcep18194_A4483

Alternate gene names: 78065954

Gene position: 1449580-1447913 (Counterclockwise)

Preceding gene: 78065955

Following gene: 78065953

Centisome position: 39.24

GC content: 68.23

Gene sequence:

>1668_bases
ATGAATACCGAATCGAGCGCCCACGGCGACAGCTCCGTCGAACACTGTGACGTCGCCATCGTCGGCTACGGCCCGACCGG
CCTCGTCGCCGCATCGATGCTGGGCCGCGCCGGCCATCGCGTGATCGTCCTCGAACGCTGGCCGACGCCCTACGGCCTGC
CGCGCCTCACGCACATCGACGGCGAAACCGCACGCATCGTGCAGGCGAGTGCCGACGTCGATCACGCGCTGCGCAATGCG
AAAGCCGTCGACACCTACCATTACTGCGATGCCAACGGCGACCTGCTGCTCGAACTGAACTGGACCGGCCGCGCGTGCGG
CTACCCCGCGCACATCTCGATCTACCAGCCCGACATCGAGGACGCGATCGATGCGCGCGCCAGCACCTTCAGGAATGTGA
CGATCCTGCGCGGCTGGGAAGTCGACACGCTGCTGCAGGACGACGACGGCGTGACGCTGACCGCCCATCCGGCGCGCGGC
AGCCAGGACGCGCAATGGACCGGCAAGCCGCGCGCCATTCGCGCCCGCTACGTGATCGGCGCCGACGGCGCGAACAGCTT
CGTGCGCCGCACGCTCGGCATCGAACGATCCGATTTCGGCTACAACGAGCGCTGGCTCAACCTCGATTCGGAGAACAAGC
GCGATCTCGGCGAAGGCTGCGCACGCACGACGATCTACTGCGATCCGGATCGCGCGTACATGCACATGCCAATTGGCACG
AAGCGCACGCGCTTCGAGCTGCGCGTGCTGCCGAACGAATCGACCGCCGAATGGGAAACGGAAGAAGCCGGCTGGAAGTG
GCTCAACGACCACTACGGCTTCGGCCCGGACGACCTGAAGCTGCTGCGCCACGTCGTCTACACGTTCGAGACGCGCATGG
CCGAACGCTGGCGCACCGGCCGCGTGCTGCTCGCGGGCGATGCGGCGCACACGATGATGCCGTACATGGGACAAGGCGCG
TGCTCCGGCATGCGCGACGGCATCAACCTCGCGTGGAAGCTCGACCTGGTGCTGACCGGGCGCGCCTGCGCCGACCTGCT
CGACACGTTCGAAGCCGAGCGCCGCCCGCACGTGACCGCGATCACGCAGATGTCGCTGTTTCTCGGGCAAGTCGTCAACG
AGGACGATCCGCGCAAGGTCGCCGAGCGCGACGCGGCGTTCCGCTCCGGCAACATGCCGCCGATGCCGCCGTTCCCGAAG
CTCGACCACGGCATCCTGCATGACGAAGCCGACGGCACGCGCCATCCGACGACCGGCGCACCCGCGCCGCAGGGACGCGT
GCGCCGCGGCGCAGCCGAGGGCCGTTTCGACGATGTGGTCGGCCGGGGATTCCAGCTCGTCACGCGCGAACACCCTTCCC
GCTACCTCGACGACGCGCAGCGTGCGTTCCTCGCGCGGCTCGGCTGTCACGTGGCCGTGCTGTCCGACGACGTGAGCGCA
CCGGATGCCGTGGTCGATCTCGACGGCGAGCAGCAAGCGTTCATGCAGGCGCACGGGATCGGCGCGTATATCGCGCGGCC
CGATTTCATCGTGTTCGGTTCGGTGGCCGAGCTGCGCGACCTCGGCGCGCTCATCGATGCACTCCGCGACAAGCTGCACT
GGTCCGCCGCGTCGACCGAGCCGTCCGCGACTTCAGCAGCCTCGGCGGCCGTCACTCACGCATCGTAA

Upstream 100 bases:

>100_bases
TGCTGCAGGACGCCTTCGACGGCAACCTGCCCGGCTCGACCGCACGCTGAACCGGCCACACGACAGACCAACAAACGAAA
CCGTCATGGAGACACCCCGC

Downstream 100 bases:

>100_bases
AGGAAACCTACGCATGACGACCGAACTGTCACCGTTCGCGCTGTCCCTGCAACGCGCGATGCCCGTCACGCGCTTGCTCG
ACTGCGGAATGGACTATGCG

Product: 3-(3-hydroxyphenyl)propionate hydroxylase

Products: tetrachlorohydroquinone; NADP+; chloride

Alternate protein names: 3-HCI hydroxylase; 3-HPP hydroxylase; Flavin-type monooxygenase [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MNTESSAHGDSSVEHCDVAIVGYGPTGLVAASMLGRAGHRVIVLERWPTPYGLPRLTHIDGETARIVQASADVDHALRNA
KAVDTYHYCDANGDLLLELNWTGRACGYPAHISIYQPDIEDAIDARASTFRNVTILRGWEVDTLLQDDDGVTLTAHPARG
SQDAQWTGKPRAIRARYVIGADGANSFVRRTLGIERSDFGYNERWLNLDSENKRDLGEGCARTTIYCDPDRAYMHMPIGT
KRTRFELRVLPNESTAEWETEEAGWKWLNDHYGFGPDDLKLLRHVVYTFETRMAERWRTGRVLLAGDAAHTMMPYMGQGA
CSGMRDGINLAWKLDLVLTGRACADLLDTFEAERRPHVTAITQMSLFLGQVVNEDDPRKVAERDAAFRSGNMPPMPPFPK
LDHGILHDEADGTRHPTTGAPAPQGRVRRGAAEGRFDDVVGRGFQLVTREHPSRYLDDAQRAFLARLGCHVAVLSDDVSA
PDAVVDLDGEQQAFMQAHGIGAYIARPDFIVFGSVAELRDLGALIDALRDKLHWSAASTEPSATSAASAAVTHAS

Sequences:

>Translated_555_residues
MNTESSAHGDSSVEHCDVAIVGYGPTGLVAASMLGRAGHRVIVLERWPTPYGLPRLTHIDGETARIVQASADVDHALRNA
KAVDTYHYCDANGDLLLELNWTGRACGYPAHISIYQPDIEDAIDARASTFRNVTILRGWEVDTLLQDDDGVTLTAHPARG
SQDAQWTGKPRAIRARYVIGADGANSFVRRTLGIERSDFGYNERWLNLDSENKRDLGEGCARTTIYCDPDRAYMHMPIGT
KRTRFELRVLPNESTAEWETEEAGWKWLNDHYGFGPDDLKLLRHVVYTFETRMAERWRTGRVLLAGDAAHTMMPYMGQGA
CSGMRDGINLAWKLDLVLTGRACADLLDTFEAERRPHVTAITQMSLFLGQVVNEDDPRKVAERDAAFRSGNMPPMPPFPK
LDHGILHDEADGTRHPTTGAPAPQGRVRRGAAEGRFDDVVGRGFQLVTREHPSRYLDDAQRAFLARLGCHVAVLSDDVSA
PDAVVDLDGEQQAFMQAHGIGAYIARPDFIVFGSVAELRDLGALIDALRDKLHWSAASTEPSATSAASAAVTHAS
>Mature_555_residues
MNTESSAHGDSSVEHCDVAIVGYGPTGLVAASMLGRAGHRVIVLERWPTPYGLPRLTHIDGETARIVQASADVDHALRNA
KAVDTYHYCDANGDLLLELNWTGRACGYPAHISIYQPDIEDAIDARASTFRNVTILRGWEVDTLLQDDDGVTLTAHPARG
SQDAQWTGKPRAIRARYVIGADGANSFVRRTLGIERSDFGYNERWLNLDSENKRDLGEGCARTTIYCDPDRAYMHMPIGT
KRTRFELRVLPNESTAEWETEEAGWKWLNDHYGFGPDDLKLLRHVVYTFETRMAERWRTGRVLLAGDAAHTMMPYMGQGA
CSGMRDGINLAWKLDLVLTGRACADLLDTFEAERRPHVTAITQMSLFLGQVVNEDDPRKVAERDAAFRSGNMPPMPPFPK
LDHGILHDEADGTRHPTTGAPAPQGRVRRGAAEGRFDDVVGRGFQLVTREHPSRYLDDAQRAFLARLGCHVAVLSDDVSA
PDAVVDLDGEQQAFMQAHGIGAYIARPDFIVFGSVAELRDLGALIDALRDKLHWSAASTEPSATSAASAAVTHAS

Specific function: Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3- hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=541, Percent_Identity=30.1293900184843, Blast_Score=200, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.50

Molecular weight: Translated: 61080; Mature: 61080

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTESSAHGDSSVEHCDVAIVGYGPTGLVAASMLGRAGHRVIVLERWPTPYGLPRLTHID
CCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECC
GETARIVQASADVDHALRNAKAVDTYHYCDANGDLLLELNWTGRACGYPAHISIYQPDIE
CCCEEEEEECCCHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCHH
DAIDARASTFRNVTILRGWEVDTLLQDDDGVTLTAHPARGSQDAQWTGKPRAIRARYVIG
HHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEE
ADGANSFVRRTLGIERSDFGYNERWLNLDSENKRDLGEGCARTTIYCDPDRAYMHMPIGT
CCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHCCHHCCEEEECCCCCEEECCCCC
KRTRFELRVLPNESTAEWETEEAGWKWLNDHYGFGPDDLKLLRHVVYTFETRMAERWRTG
CCEEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
RVLLAGDAAHTMMPYMGQGACSGMRDGINLAWKLDLVLTGRACADLLDTFEAERRPHVTA
CEEEECCHHHHHHCCCCCCHHCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCHHH
ITQMSLFLGQVVNEDDPRKVAERDAAFRSGNMPPMPPFPKLDHGILHDEADGTRHPTTGA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPQGRVRRGAAEGRFDDVVGRGFQLVTREHPSRYLDDAQRAFLARLGCHVAVLSDDVSA
CCCCCHHHCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCC
PDAVVDLDGEQQAFMQAHGIGAYIARPDFIVFGSVAELRDLGALIDALRDKLHWSAASTE
CCEEEECCCCHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCC
PSATSAASAAVTHAS
CCCHHHHHHEEECCC
>Mature Secondary Structure
MNTESSAHGDSSVEHCDVAIVGYGPTGLVAASMLGRAGHRVIVLERWPTPYGLPRLTHID
CCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECC
GETARIVQASADVDHALRNAKAVDTYHYCDANGDLLLELNWTGRACGYPAHISIYQPDIE
CCCEEEEEECCCHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCHH
DAIDARASTFRNVTILRGWEVDTLLQDDDGVTLTAHPARGSQDAQWTGKPRAIRARYVIG
HHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEE
ADGANSFVRRTLGIERSDFGYNERWLNLDSENKRDLGEGCARTTIYCDPDRAYMHMPIGT
CCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHCCHHCCEEEECCCCCEEECCCCC
KRTRFELRVLPNESTAEWETEEAGWKWLNDHYGFGPDDLKLLRHVVYTFETRMAERWRTG
CCEEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
RVLLAGDAAHTMMPYMGQGACSGMRDGINLAWKLDLVLTGRACADLLDTFEAERRPHVTA
CEEEECCHHHHHHCCCCCCHHCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCHHH
ITQMSLFLGQVVNEDDPRKVAERDAAFRSGNMPPMPPFPKLDHGILHDEADGTRHPTTGA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPQGRVRRGAAEGRFDDVVGRGFQLVTREHPSRYLDDAQRAFLARLGCHVAVLSDDVSA
CCCCCHHHCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCC
PDAVVDLDGEQQAFMQAHGIGAYIARPDFIVFGSVAELRDLGALIDALRDKLHWSAASTE
CCEEEECCCCHHHHHHHCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCC
PSATSAASAAVTHAS
CCCHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pentachlorophenol; NADPH; H+; O2

Specific reaction: pentachlorophenol + NADPH + H+ + O2 = tetrachlorohydroquinone + NADP+ + chloride

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA