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The map label for this gene is phoB [H]

Identifier: 78065919

GI number: 78065919

Start: 1406890

End: 1407591

Strand: Direct

Name: phoB [H]

Synonym: Bcep18194_A4448

Alternate gene names: 78065919

Gene position: 1406890-1407591 (Clockwise)

Preceding gene: 78065918

Following gene: 78065920

Centisome position: 38.08

GC content: 64.81

Gene sequence:

>702_bases
ATGCCCAGCAACATTCTCGTCGTTGAAGATGAGCCCGCGATTTCCGAACTGATCTCGGTGAATCTCCAGCACGCCGGTCA
CTGCCCGATCCGCGCGTACAACGCCGAACAGGCTCAAAACCTGATCAGCGACGTGCTGCCCGATCTCGTGCTGCTCGACT
GGATGCTGCCGGGCAAGTCCGGTATCGCGTTCGCGCGCGACCTGCGCAACAACGAACGGACCAAGCACATCCCGATCATC
ATGCTGACTGCCCGCGGCGACGAGCAGGACAAGGTGCTCGGCCTCGAAATCGGCGCGGACGACTACGTGACGAAGCCGTT
TTCGCCGAAGGAGCTGATGGCGCGGATCAAGGCCGTGCTGCGCCGCCGCGCGCCGCAGCTGACCGAGGACGTCGTGGCGA
TCAACGGGCTGCGCCTCGACCCGGCCACGCATCGCGTCGCCGCGCATGCGGAAGGCAGCGAGATCAAGCTCGATCTCGGC
CCGACCGAGTTCCGCCTGCTGCATTTCTTCATGACGCATCCGGAGCGCGTGCACAGCCGCACGCAACTGCTCGACCAGGT
GTGGGGCGACCATGTATTCGTCGAGGAGCGCACCGTCGACGTGCACATCAAACGATTGCGCGCGGCCTTGAAACCGGCCG
GCTGCGATGCGATGATCGAGACCGTGCGCGGCAGCGGCTACCGGCTCGCCAAGCACGCGTAA

Upstream 100 bases:

>100_bases
TACATCGTGAAGGGCACCGACGTGCGGCACCAGCCGCGCGACACGCTCGATCGCGAAGCCAACAGTTAATCGACTCCGTA
CAGGAAGAATAGAGGTGCCG

Downstream 100 bases:

>100_bases
CGTATCCACGCATGCCGTGTGCCACGGCATGCCGTTCATTCTTTCGGGCGCTGAATCATGAACATCATCTGGGCGCGCTT
TCTGGTGTCGCTCGTGCTGC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVAINGLRLDPATHRVAAHAEGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA

Sequences:

>Translated_233_residues
MPSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVAINGLRLDPATHRVAAHAEGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA
>Mature_232_residues
PSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPIIM
LTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVAINGLRLDPATHRVAAHAEGSEIKLDLGP
TEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=230, Percent_Identity=58.2608695652174, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788394, Length=231, Percent_Identity=41.991341991342, Blast_Score=163, Evalue=9e-42,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=38.7931034482759, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=36.4444444444444, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=38.5964912280702, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=33.7606837606838, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=33.6322869955157, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786911, Length=233, Percent_Identity=35.6223175965665, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=34.375, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=39.3162393162393, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=33.7552742616034, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=33.4801762114537, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI145693140, Length=244, Percent_Identity=31.9672131147541, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788550, Length=113, Percent_Identity=35.3982300884956, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26088; Mature: 25957

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKS
CCCCEEEECCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVAINGLRLDPATHRVAAHAEGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCCHHHHHHHHCCEEECCHHHHEEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure 
PSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKS
CCCEEEECCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVAINGLRLDPATHRVAAHAEGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCCHHHHHHHHCCEEECCHHHHEEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]