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The map label for this gene is rlmE [H]

Identifier: 78065909

GI number: 78065909

Start: 1396727

End: 1397389

Strand: Direct

Name: rlmE [H]

Synonym: Bcep18194_A4438

Alternate gene names: 78065909

Gene position: 1396727-1397389 (Clockwise)

Preceding gene: 78065907

Following gene: 78065910

Centisome position: 37.81

GC content: 62.29

Gene sequence:

>663_bases
ATGGCAAAAAACCGCTTCAACCAGCACTGGCTGCACGACCACATCAACGACCCCTACGTCAAAATGGCGCAGCGGGAGGG
CTATCGCGCGCGCGCCGCGTACAAGCTGAAGGAAATCGACGAGCAGGACAAGCTGATCCGTCCGGGCCAGGTGATCGTCG
ACCTCGGCGCGACGCCGGGCAGCTGGAGCCAGTATGCCCGCAACAAGCTCGCGCAGGGCAAGAAGCGCGACACGGAGCGC
GAAGGCGGCATCGACGGCACGATCGTGGCGCTCGACATCCTGCCGATGGAGCCGATCGCCGACGTCCACTTCCTCCAGGG
CGACTTCCGCGAGGACGAAGTCCTTCACCAGCTCGAAGAAGTGCTCGAAGGGCGCGACGTGGACCTTGTTATTTCCGACA
TGGCCCCCAACCTCTCTGGCGTGGCCTCGGCGGACGCGGCGCGCATCGAGCATCTATGCGATCTGGCGCTCGAATTCGCG
CAGAATCATCTGAAGCCGGACGGTGCGCTGCTCGTGAAATGCTTTCACGGCAGCGGCTACAGCCAGATCGTCGAGAAATT
CAAACAGCAGTTTAAGACCGTCGCGCCGCGCAAGCCGAAGGCGTCCCGCGACAAATCGTCCGAAACGTTCATTTTGGGTC
GGCATCTGAAACGTCCGCGCTGA

Upstream 100 bases:

>100_bases
GGCTTGGGCCTGAAAAGGGGCCGCAGCCGCGCGAAGAAGACGCGTATTATCCGCTAAAAGCGCGGCTTCACGAAGCAATT
AGCAGCAATCTCTCTCTCGA

Downstream 100 bases:

>100_bases
TGCGCAACGGGGTGCCGCAAACGGGCTCCGGCCCTTGCCAGACAAGCGAAGCGCTATCGCGGCGGTTGTCAATGCTTATG
GCGAGGGTGGTCGGACTGGA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPGSWSQYARNKLAQGKKRDTER
EGGIDGTIVALDILPMEPIADVHFLQGDFREDEVLHQLEEVLEGRDVDLVISDMAPNLSGVASADAARIEHLCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKQQFKTVAPRKPKASRDKSSETFILGRHLKRPR

Sequences:

>Translated_220_residues
MAKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPGSWSQYARNKLAQGKKRDTER
EGGIDGTIVALDILPMEPIADVHFLQGDFREDEVLHQLEEVLEGRDVDLVISDMAPNLSGVASADAARIEHLCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKQQFKTVAPRKPKASRDKSSETFILGRHLKRPR
>Mature_219_residues
AKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPGSWSQYARNKLAQGKKRDTERE
GGIDGTIVALDILPMEPIADVHFLQGDFREDEVLHQLEEVLEGRDVDLVISDMAPNLSGVASADAARIEHLCDLALEFAQ
NHLKPDGALLVKCFHGSGYSQIVEKFKQQFKTVAPRKPKASRDKSSETFILGRHLKRPR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=216, Percent_Identity=36.1111111111111, Blast_Score=139, Evalue=1e-33,
Organism=Homo sapiens, GI194097365, Length=203, Percent_Identity=29.5566502463054, Blast_Score=107, Evalue=6e-24,
Organism=Homo sapiens, GI29029591, Length=203, Percent_Identity=31.5270935960591, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI29029589, Length=203, Percent_Identity=31.5270935960591, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI7110661, Length=203, Percent_Identity=31.5270935960591, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=42.7184466019417, Blast_Score=184, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17553474, Length=226, Percent_Identity=32.3008849557522, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17554650, Length=203, Percent_Identity=34.4827586206897, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=203, Percent_Identity=31.5270935960591, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=203, Percent_Identity=31.5270935960591, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=203, Percent_Identity=31.5270935960591, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=31.219512195122, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=32.6633165829146, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=291, Percent_Identity=26.4604810996564, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=33.9449541284404, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24648639, Length=203, Percent_Identity=32.0197044334975, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24647580, Length=204, Percent_Identity=32.843137254902, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI18859957, Length=196, Percent_Identity=29.0816326530612, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24906; Mature: 24774

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPG
CCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGKKRDTEREGGIDGTIVALDILPMEPIADVHFLQGDFREDEVLHQLEE
CHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEEECCCCCCCHHHHHCCCCCHHHHHHHHHH
VLEGRDVDLVISDMAPNLSGVASADAARIEHLCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKQQFKTVAPRKPKASRDKSSETFILGRHLKRPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHCCCCC
>Mature Secondary Structure 
AKNRFNQHWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGATPG
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGKKRDTEREGGIDGTIVALDILPMEPIADVHFLQGDFREDEVLHQLEE
CHHHHHHHHHHCCCCCCCHHCCCCCCEEEEEEECCCCCCCHHHHHCCCCCHHHHHHHHHH
VLEGRDVDLVISDMAPNLSGVASADAARIEHLCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKQQFKTVAPRKPKASRDKSSETFILGRHLKRPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA