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The map label for this gene is lepA [H]

Identifier: 78065716

GI number: 78065716

Start: 1177722

End: 1179515

Strand: Direct

Name: lepA [H]

Synonym: Bcep18194_A4244

Alternate gene names: 78065716

Gene position: 1177722-1179515 (Clockwise)

Preceding gene: 78065715

Following gene: 78065717

Centisome position: 31.88

GC content: 62.04

Gene sequence:

>1794_bases
ATGGATCATATTCGCAATTTCTCGATCATCGCGCACATCGACCATGGCAAGTCGACGCTCGCGGATCGCATCATCCAGGT
ATGCGGCGGCCTCGCCGACCGTGAAATGGAAGCGCAGGTGCTCGACTCGATGGATATCGAGCGCGAGCGCGGCATCACGA
TCAAGGCGCAGACTGCCGCGCTGTCCTATCGCGCGCGCGACGGCAAGGTCTACAACCTGAACCTGATCGACACGCCGGGG
CACGTCGACTTCTCGTACGAAGTCAGCCGTTCGCTGTCCGCATGCGAAGGCGCGCTGCTGGTCGTCGATGCGAGCCAGGG
CGTCGAGGCGCAGACGGTCGCGAACTGCTACACGGCGATCGAGCTCGGCGTCGAGGTCGTGCCCGTGCTGAACAAGATCG
ACCTGCCCGCCGCGAACCCCGAAAACGCGATCGAGGAGATCGAGGACGTGATCGGCATCGACGCGACCGACGCGACGCGT
TGCAGCGCGAAGACGGGTCTGGGCGTCGAGGACGTGCTCGAGTCGCTGATCGCGAAGGTGCCGCCGCCGAAGGGCGATCC
GGCCGCGCCGCTGCAGGCGCTCATCATCGATTCGTGGTTCGACAACTACGTCGGCGTCGTGATGCTCGTACGCATCGTTA
ACGGCACGCTGCGCCCGAAGGACAAGATCAAGCTGATGGCGACCGGCGCGCAGTATCCGGTCGAGCACATCGGCGTGTTC
ACGCCGAAGTCGCGCAATCTCGAAACGCTGTCGGCCGGGCAGGTGGGTTTCATCATCGCTGGCATCAAGGAACTGACGGC
AGCGAAGGTCGGCGACACCGTCACGCACGCGACCAAGGCGGCTGTCGAGCCGCTGCCGGGCTTCAAGGAAGTGAAGCCGC
AGGTGTTCGCGGGGCTGTATCCGGTCGAGGCTAACCAGTACGACGCACTGCGCGAATCGCTCGAGAAGCTGAAGTTGAAC
GATGCGTCGCTGCAGTACGAGCCGGAAGTGTCGCAGGCGCTCGGCTTCGGTTTCCGCTGCGGCTTCCTTGGCCTGCTGCA
CATGGAAATCGTGCAGGAGCGGCTCGAGCGCGAGTTCGACATGGACCTCATCACGACCGCGCCGACGGTTGTCTACGAGG
TCATGATGAGCGACGGCGCGATCATCAAGGTCGAGAATCCGGCGAAGATGCCGGAGCCGCCGAGGATCGAGGAGATCCGC
GAGCCGATCGTCACGGTGAACCTGTACATGCCGCAGGACTACGTCGGCTCCGTGATCACGCTGTGCGAGCAGAAGCGCGG
ATCGCAGATCAACATGCAATATCACGGCCGTCAGGTGCAACTGACCTACGAGATCCCGATGGCCGAGATCGTGCTCGATT
TCTTCGATCGCCTGAAGTCGGTGTCGCGCGGCTACGCGTCGATGGACTACGAGTTCAAGGAATACCGCGCGGCCGATGTC
GTGAAGGTCGACATGCTGATCAACGGTGACAAGGTCGATGCGTTGTCGGTTATCGTCCACCGTTCGCAGTCGCAGTACCG
CGGCCGCGAAGTGGCCGCGAAGATGCGCGAGATCATTCCACGCCAGATGTACGACGTGGCGATCCAGGCTACGATCGGCG
CGCACATCATTGCCCGCGAGAACATCAAGGCGCTGCGCAAGAACGTGTTGGCGAAGTGCTATGGCGGCGACATCTCGCGA
AAGAAGAAACTGCTCGAAAAGCAGAAAGCGGGTAAGAAACGAATGAAGCAGGTGGGCTCGGTCGAGATCCCGCAGGAAGC
GTTCCTTGCCATCTTGCGCGTCGAAGACAAATAA

Upstream 100 bases:

>100_bases
GGCAAAGGCCTTTTCGGCTAAAATAAGCTGTTTTTTCACCGACTTACCAAGGCGTGCTCTGCAGTCGTCGAGCGCGCCTT
TTTCGCTTGATCGGCACTGA

Downstream 100 bases:

>100_bases
CAGGACTGATCCTTTTATGAATTTTGCGCTGATTCTTTTTGTGCTCGTCGTCTTGACGGGCGTAGCGTGGGTGTTGGACA
AGCTGGTGTTCCTGCCGCAG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLADREMEAQVLDSMDIERERGITIKAQTAALSYRARDGKVYNLNLIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPENAIEEIEDVIGIDATDATR
CSAKTGLGVEDVLESLIAKVPPPKGDPAAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQYPVEHIGVF
TPKSRNLETLSAGQVGFIIAGIKELTAAKVGDTVTHATKAAVEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVMMSDGAIIKVENPAKMPEPPRIEEIR
EPIVTVNLYMPQDYVGSVITLCEQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRAADV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQATIGAHIIARENIKALRKNVLAKCYGGDISR
KKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK

Sequences:

>Translated_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLADREMEAQVLDSMDIERERGITIKAQTAALSYRARDGKVYNLNLIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPENAIEEIEDVIGIDATDATR
CSAKTGLGVEDVLESLIAKVPPPKGDPAAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQYPVEHIGVF
TPKSRNLETLSAGQVGFIIAGIKELTAAKVGDTVTHATKAAVEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVMMSDGAIIKVENPAKMPEPPRIEEIR
EPIVTVNLYMPQDYVGSVITLCEQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRAADV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQATIGAHIIARENIKALRKNVLAKCYGGDISR
KKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK
>Mature_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLADREMEAQVLDSMDIERERGITIKAQTAALSYRARDGKVYNLNLIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPENAIEEIEDVIGIDATDATR
CSAKTGLGVEDVLESLIAKVPPPKGDPAAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQYPVEHIGVF
TPKSRNLETLSAGQVGFIIAGIKELTAAKVGDTVTHATKAAVEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVMMSDGAIIKVENPAKMPEPPRIEEIR
EPIVTVNLYMPQDYVGSVITLCEQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRAADV
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQATIGAHIIARENIKALRKNVLAKCYGGDISR
KKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=48.841059602649, Blast_Score=615, Evalue=1e-176,
Organism=Homo sapiens, GI18390331, Length=160, Percent_Identity=38.75, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.1127819548872, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=46.3087248322148, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI25306287, Length=149, Percent_Identity=46.3087248322148, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI19923640, Length=149, Percent_Identity=46.3087248322148, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=42.3611111111111, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=42.7272727272727, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=42.7272727272727, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=42.7272727272727, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI217272894, Length=167, Percent_Identity=29.940119760479, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI217272892, Length=167, Percent_Identity=29.940119760479, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI53729339, Length=218, Percent_Identity=28.4403669724771, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI53729337, Length=218, Percent_Identity=28.4403669724771, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI4503475, Length=156, Percent_Identity=33.974358974359, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI4503471, Length=173, Percent_Identity=31.7919075144509, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=70.03367003367, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=514, Percent_Identity=28.5992217898833, Blast_Score=173, Evalue=4e-44,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=37.4193548387097, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.6923076923077, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789559, Length=224, Percent_Identity=29.0178571428571, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=39.3790849673203, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17556745, Length=469, Percent_Identity=26.226012793177, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17533571, Length=161, Percent_Identity=34.1614906832298, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=39.7435897435897, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.0597014925373, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=38.0597014925373, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=35.3383458646617, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=312, Percent_Identity=27.8846153846154, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI32566303, Length=244, Percent_Identity=28.2786885245902, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=221, Percent_Identity=27.6018099547511, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=47.5083056478405, Blast_Score=572, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6323098, Length=160, Percent_Identity=40, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=42.3611111111111, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=42.3611111111111, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=42.6086956521739, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=38.1944444444444, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=156, Percent_Identity=31.4102564102564, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=156, Percent_Identity=31.4102564102564, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=319, Percent_Identity=24.1379310344828, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=45.6810631229236, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=37.888198757764, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=38.5135135135135, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=38.5135135135135, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=38.5135135135135, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=43.0656934306569, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.255033557047, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=34.5864661654135, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=219, Percent_Identity=28.310502283105, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66044; Mature: 66044

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLADREMEAQVLDSMDIERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEHHE
LSYRARDGKVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVEVVPVLNKIDLPAANPENAIEEIEDVIGIDATDATRCSAKTGLGVEDVLESLIAKV
HHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PPPKGDPAAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQYPVEHIGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHCEEE
TPKSRNLETLSAGQVGFIIAGIKELTAAKVGDTVTHATKAAVEPLPGFKEVKPQVFAGLY
CCCCCCCCEECCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCHHHHHCCC
PVEANQYDALRESLEKLKLNDASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVMMSDGAIIKVENPAKMPEPPRIEEIREPIVTVNLYMPQDYVGSVIT
CHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHCCCEEEEEEECCHHHHHHHHH
LCEQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRAADV
HHHHHCCCEEEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCE
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQATIGAHIIARE
EEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHH
NIKALRKNVLAKCYGGDISRKKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC
>Mature Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQVCGGLADREMEAQVLDSMDIERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEHHE
LSYRARDGKVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVEVVPVLNKIDLPAANPENAIEEIEDVIGIDATDATRCSAKTGLGVEDVLESLIAKV
HHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PPPKGDPAAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIKLMATGAQYPVEHIGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHCEEE
TPKSRNLETLSAGQVGFIIAGIKELTAAKVGDTVTHATKAAVEPLPGFKEVKPQVFAGLY
CCCCCCCCEECCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCHHHHHCCC
PVEANQYDALRESLEKLKLNDASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVMMSDGAIIKVENPAKMPEPPRIEEIREPIVTVNLYMPQDYVGSVIT
CHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHCCCEEEEEEECCHHHHHHHHH
LCEQKRGSQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRAADV
HHHHHCCCEEEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCE
VKVDMLINGDKVDALSVIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQATIGAHIIARE
EEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHH
NIKALRKNVLAKCYGGDISRKKKLLEKQKAGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA