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The map label for this gene is pur8 [H]

Identifier: 78065409

GI number: 78065409

Start: 855248

End: 856687

Strand: Reverse

Name: pur8 [H]

Synonym: Bcep18194_A3935

Alternate gene names: 78065409

Gene position: 856687-855248 (Counterclockwise)

Preceding gene: 78065410

Following gene: 78065408

Centisome position: 23.19

GC content: 70.83

Gene sequence:

>1440_bases
ATGACCCACGGGATTCACGGCGAGAAGCGCTGGTACGCGCTGATCGTCCTCTGCCTCGGCGTGCTGATGATCGTGCTCGA
CAGCACGATCGTGAACGTTGCACTGCCGTCGATCAGCACCGACCTCCACTTCACCGAGACGGCCCTCGTGTGGGTCGTCA
ACGCGTACCTGCTGACGTTCGGCGGCTGCCTGCTGCTCGGCGGACGGCTCGGCGACCTGTACGGCCAGCGGCGCATGTTC
CTCGCCGGCCTCGTCGTCTTCACGCTCGCATCGCTTGCGTGCGGCCTCGCGCAGTCGCAGACGATGCTGATCGCCGCGCG
CGCGGTGCAGGGGATCGGCGGTGCAGTCGTATCGGCCGTCGCGCTGTCGCTGATCATGAATCTCTTCACCGAGCCCGGCG
AGCGCGCCCGCGCGATGGGCGTGTACGGCTTCGTCTGCGCCGGCGGCGGCAGCATCGGCGTGCTGCTCGGCGGGCTGCTG
ACGAGCTCGCTGTCGTGGCACTGGATCTTCCTCGTCAACCTGCCGATCGGCATCGCGGTCTATGCGATGTGCGTCGCGCT
GCTGCCGCGCCTGCGCGCGCCGGCCGGCACCGCGCGGCTCGACGTCGCGGGTGCGATCACCGTCACTGCATCGCTGATGC
TGGCCGTCTACGGGATCGTCGGCGGCAACGAGGCCGGCTGGCTGTCGACGCAGACCGTTTCACTGATCGGCGCGGCCGTC
GTGCTGCTCGCGCTGTTCATCGCGATCGAGGCGCGCGCCGCGCATCCGCTGATGCCGCTCTCGCTGTTCGCCTCGCGCAA
CGTCGCGCTCGCGAACGTGATCGCCGTGCTGTGGGCGGCCGCGATGTTCGCGTGGTTCTTCCTGTCCGCGCTGTACATGC
AGCGCGTGCTCGGCTACGGGCCGCTGCAGGTCGGCCTCGCGTTCCTGCCGGCGAACCTGATCATGGCCGTGTTCTCGCTC
GGGCTGTCGGCACGCATCGTGATGCGCTTCGGGATCCGCGGCCCGATCGCCGCCGGCCTGCTGATCGCGGCCTGCGGCCT
CGCGCTGTTCTCGCGCGCACCGGTGGACGGCGGCTTCGTCTGGCACGTGCTGCCCGGCATGACGCTGCTCGGCATCGGCG
CGGGCGTCGCGTTCAATCCGATGCTGCTCGCCGCGATGAGCGACGTCGATCCGGCGGATTCCGGGCTCGCGTCGGGGATC
GTCAACACCGCGTTCATGATGGGCGGCGCACTCGGCCTCGCCGTGCTCGCGAGCCTCGCCGCCGCCCGCACCGATGCACT
CGCGGCCGCGAACGCCGCGCCGCTCGACGCGCTGAACGGCGGCTACCATGCGGCCTTCGCGTTCGGCGCGGCATTCGCTG
CCGCCGCCGCGCTGATCGGCCTCGCGCTGCGCATCCGGCGGCAGGGAGCGGTCGAAGGCGTCGGCCCCGCGATGCACTGA

Upstream 100 bases:

>100_bases
GCGCGACTACGCATCGCTGCTGCCACAAACCGGCAGGAAGCCCGCAGCGATGGGGCCTACAATGCATCCTTTCAAATAAC
TATCAGAAGGCTGCAAGCCC

Downstream 100 bases:

>100_bases
CCGGTAGCCGGCGGCTGGCGGCGTCCGCCGTCGCTATCGAACCAGGGCGCGCATCGTCGCGCCCTTTTTTACGCGTGCCT
GGCCCGGCACCCGCGCTCGG

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTHGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSISTDLHFTETALVWVVNAYLLTFGGCLLLGGRLGDLYGQRRMF
LAGLVVFTLASLACGLAQSQTMLIAARAVQGIGGAVVSAVALSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLL
TSSLSWHWIFLVNLPIGIAVYAMCVALLPRLRAPAGTARLDVAGAITVTASLMLAVYGIVGGNEAGWLSTQTVSLIGAAV
VLLALFIAIEARAAHPLMPLSLFASRNVALANVIAVLWAAAMFAWFFLSALYMQRVLGYGPLQVGLAFLPANLIMAVFSL
GLSARIVMRFGIRGPIAAGLLIAACGLALFSRAPVDGGFVWHVLPGMTLLGIGAGVAFNPMLLAAMSDVDPADSGLASGI
VNTAFMMGGALGLAVLASLAAARTDALAAANAAPLDALNGGYHAAFAFGAAFAAAAALIGLALRIRRQGAVEGVGPAMH

Sequences:

>Translated_479_residues
MTHGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSISTDLHFTETALVWVVNAYLLTFGGCLLLGGRLGDLYGQRRMF
LAGLVVFTLASLACGLAQSQTMLIAARAVQGIGGAVVSAVALSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLL
TSSLSWHWIFLVNLPIGIAVYAMCVALLPRLRAPAGTARLDVAGAITVTASLMLAVYGIVGGNEAGWLSTQTVSLIGAAV
VLLALFIAIEARAAHPLMPLSLFASRNVALANVIAVLWAAAMFAWFFLSALYMQRVLGYGPLQVGLAFLPANLIMAVFSL
GLSARIVMRFGIRGPIAAGLLIAACGLALFSRAPVDGGFVWHVLPGMTLLGIGAGVAFNPMLLAAMSDVDPADSGLASGI
VNTAFMMGGALGLAVLASLAAARTDALAAANAAPLDALNGGYHAAFAFGAAFAAAAALIGLALRIRRQGAVEGVGPAMH
>Mature_478_residues
THGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSISTDLHFTETALVWVVNAYLLTFGGCLLLGGRLGDLYGQRRMFL
AGLVVFTLASLACGLAQSQTMLIAARAVQGIGGAVVSAVALSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLLT
SSLSWHWIFLVNLPIGIAVYAMCVALLPRLRAPAGTARLDVAGAITVTASLMLAVYGIVGGNEAGWLSTQTVSLIGAAVV
LLALFIAIEARAAHPLMPLSLFASRNVALANVIAVLWAAAMFAWFFLSALYMQRVLGYGPLQVGLAFLPANLIMAVFSLG
LSARIVMRFGIRGPIAAGLLIAACGLALFSRAPVDGGFVWHVLPGMTLLGIGAGVAFNPMLLAAMSDVDPADSGLASGIV
NTAFMMGGALGLAVLASLAAARTDALAAANAAPLDALNGGYHAAFAFGAAFAAAAALIGLALRIRRQGAVEGVGPAMH

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=33.0985915492958, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1789042, Length=476, Percent_Identity=26.6806722689076, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790195, Length=413, Percent_Identity=29.5399515738499, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1788710, Length=455, Percent_Identity=25.7142857142857, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788392, Length=392, Percent_Identity=30.1020408163265, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1790146, Length=169, Percent_Identity=26.6272189349112, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082320, Length=201, Percent_Identity=28.8557213930348, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=420, Percent_Identity=26.9047619047619, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322958, Length=386, Percent_Identity=24.3523316062176, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=287, Percent_Identity=27.8745644599303, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323520, Length=269, Percent_Identity=26.7657992565056, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=409, Percent_Identity=23.960880195599, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=127, Percent_Identity=33.8582677165354, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=323, Percent_Identity=24.7678018575851, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49098; Mature: 48967

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSISTDLHFTETALVWVVNAYLLTF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
GGCLLLGGRLGDLYGQRRMFLAGLVVFTLASLACGLAQSQTMLIAARAVQGIGGAVVSAV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHH
ALSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLLTSSLSWHWIFLVNLPIGIAV
HHHHHHHHHCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEEECCHHHHH
YAMCVALLPRLRAPAGTARLDVAGAITVTASLMLAVYGIVGGNEAGWLSTQTVSLIGAAV
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VLLALFIAIEARAAHPLMPLSLFASRNVALANVIAVLWAAAMFAWFFLSALYMQRVLGYG
HHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLQVGLAFLPANLIMAVFSLGLSARIVMRFGIRGPIAAGLLIAACGLALFSRAPVDGGFV
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
WHVLPGMTLLGIGAGVAFNPMLLAAMSDVDPADSGLASGIVNTAFMMGGALGLAVLASLA
HHHHCCHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARTDALAAANAAPLDALNGGYHAAFAFGAAFAAAAALIGLALRIRRQGAVEGVGPAMH
HHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
THGIHGEKRWYALIVLCLGVLMIVLDSTIVNVALPSISTDLHFTETALVWVVNAYLLTF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
GGCLLLGGRLGDLYGQRRMFLAGLVVFTLASLACGLAQSQTMLIAARAVQGIGGAVVSAV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHH
ALSLIMNLFTEPGERARAMGVYGFVCAGGGSIGVLLGGLLTSSLSWHWIFLVNLPIGIAV
HHHHHHHHHCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEEECCHHHHH
YAMCVALLPRLRAPAGTARLDVAGAITVTASLMLAVYGIVGGNEAGWLSTQTVSLIGAAV
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VLLALFIAIEARAAHPLMPLSLFASRNVALANVIAVLWAAAMFAWFFLSALYMQRVLGYG
HHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLQVGLAFLPANLIMAVFSLGLSARIVMRFGIRGPIAAGLLIAACGLALFSRAPVDGGFV
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
WHVLPGMTLLGIGAGVAFNPMLLAAMSDVDPADSGLASGIVNTAFMMGGALGLAVLASLA
HHHHCCHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARTDALAAANAAPLDALNGGYHAAFAFGAAFAAAAALIGLALRIRRQGAVEGVGPAMH
HHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]