Click here to switch to the map view.

The map label for this gene is pcnB [H]

Identifier: 78065319

GI number: 78065319

Start: 752223

End: 753761

Strand: Reverse

Name: pcnB [H]

Synonym: Bcep18194_A3845

Alternate gene names: 78065319

Gene position: 753761-752223 (Counterclockwise)

Preceding gene: 78065320

Following gene: 78065318

Centisome position: 20.4

GC content: 68.36

Gene sequence:

>1539_bases
GTGATCAAAAAATTCATCCGCAAGCTGCTCGGCCAGGACGAAACCGAGCAAACCTCTCCCGCAACGGCCCCGGCCGACGA
AGCGGCCCCTGCTACCCCGCGCGCCCCGAAGGGCGCCCGCGGCGGCGGCGCGAAGAAACCGCGCAGCAACCATGAACCGA
CCGTCGTGCCGGCCAGCGTGCACGGCATCAACCCGGCGCTGATTTCGAAGAATGCCGTGCGCGTGACCGACACGCTGCAG
CAGGCGGGCTTCCGTGCCTTCATCGTCGGCGGTGCGGTACGCGACCTGCTGCTCGGCATCGCGCCGAAGGACTTCGACGT
CGCGACCGATGCAACGCCGACCGAGGTGCAGCGCCTGTTCCGCCGCGCGCGCCTGATCGGCCGCCGCTTCCAGATCGTGC
ACGTGCAGTTCGGCCAGGAGCTGATCGAGGTGTCGACGTTCCGCGCGCTGGTCGATGCGCCGCCCGAGGCCGCCGCCGCC
GAGCCGCCGAAGCGCCTGAAGCGCGACGAACTCGATCGCCGCACGCACGCGGTCGACGCGAGCGGCCGCGTGCTGCGCGA
CAACGTGTGGGGCGAGCAGCACGAAGACGCCGCGCGCCGCGACTTCACGATCAACGCGATGTACTACGACCCGTCGACGC
AGACGGTGCTCGACTACCACGACGGGATGGCCGACGTGCGCGCCCGCCTGTTGCGGATGATCGGCGATCCGGCCACGCGC
TTCCGCGAGGATCCGGTGCGGATGCTGCGCGTCGTGCGCTTCGCGGCCAAGCTCGGTTTCGAGATCGAGCCGCATACGCG
CGAGCCGATCAACGCGCTCGCCGACCTGATCAACAACGTGCCGGCCGCGCGCCTGTTCGACGAAATGCTGAAGCTGCTGC
TGTCGGGCCACGCGCTCGCGTGCCTGACGCAGCTGCGCAAGGAAGGCCTGCACCACGGGCTGCTGCCGCTGCTCGACGTC
GTGCTCGAACAGCCGCAGGGCGAGAAGTTCATCACGCTCGCGCTGAACAACACCGACGCACGCGTGCGCGCCGGCAAGCC
GGTCTCGCCGGGCTTCCTGTTCGCGACGCTGCTGTGGCACGACATGCGCCAGCGCTTCGAGCAATACACGGCCGAAGGCG
AAATCCCGGTGCCGGCGCTCCATCGCGCGATGGACGACGTGCTCGACATGCAGACCGAGAAACTCGCGATCCACAAGCGT
TATTCGGCCGACATGCGCGAGATCTGGGGCCTGCAGCTGCGCCTCGAGAAGCGCTCGGGCCGCAGCGCGATGCGGCTGCT
GGAACACCAAAGGTTTAGAGCGGGGTATGATTTCCTCCTGTTACGCTGCGAATCCGGCGAGCTTGATGCGGAAGTCGGAC
AGTGGTGGACGGATTTCATCGAAGGCGACGCGGCCGCTCGCGAGGCATTGCTCACGCAGGGCGGCACGAAGGAAAAATCG
CCCCGCAAGCGGCGCCGCCGCGGCGGCGTGCGAAACCGCAAGCCGGGTGAAGGTGCTGCCGAGCAGGCACCGGACGCCTC
GGACGGTTCCGACGACTGA

Upstream 100 bases:

>100_bases
TCGAGAAAGTCACCGACCCGATCGCGACCAACCCTGACGACACGCTGCGTGCGCATGCAAGCAACCGCGGCTGGCGCATC
CTCGATCTCTTCTGAACGTC

Downstream 100 bases:

>100_bases
CGCGCTCGCCGGCGAACGACGTAGGAAGTGATGCCATGACGGTTGCATATATCGGACTGGGGGCGAATCTCGGCGATGCG
CGCCAGACCCTGAAGGACGC

Product: Poly(A) polymerase, PcnB

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MIKKFIRKLLGQDETEQTSPATAPADEAAPATPRAPKGARGGGAKKPRSNHEPTVVPASVHGINPALISKNAVRVTDTLQ
QAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAAAA
EPPKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
FREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALACLTQLRKEGLHHGLLPLLDV
VLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTAEGEIPVPALHRAMDDVLDMQTEKLAIHKR
YSADMREIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGTKEKS
PRKRRRRGGVRNRKPGEGAAEQAPDASDGSDD

Sequences:

>Translated_512_residues
MIKKFIRKLLGQDETEQTSPATAPADEAAPATPRAPKGARGGGAKKPRSNHEPTVVPASVHGINPALISKNAVRVTDTLQ
QAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAAAA
EPPKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
FREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALACLTQLRKEGLHHGLLPLLDV
VLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTAEGEIPVPALHRAMDDVLDMQTEKLAIHKR
YSADMREIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGTKEKS
PRKRRRRGGVRNRKPGEGAAEQAPDASDGSDD
>Mature_512_residues
MIKKFIRKLLGQDETEQTSPATAPADEAAPATPRAPKGARGGGAKKPRSNHEPTVVPASVHGINPALISKNAVRVTDTLQ
QAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLFRRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAAAA
EPPKRLKRDELDRRTHAVDASGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
FREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALACLTQLRKEGLHHGLLPLLDV
VLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWHDMRQRFEQYTAEGEIPVPALHRAMDDVLDMQTEKLAIHKR
YSADMREIWGLQLRLEKRSGRSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGTKEKS
PRKRRRRGGVRNRKPGEGAAEQAPDASDGSDD

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=465, Percent_Identity=42.3655913978495, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1789436, Length=254, Percent_Identity=29.5275590551181, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281360112, Length=234, Percent_Identity=29.4871794871795, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357337, Length=234, Percent_Identity=29.4871794871795, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 57192; Mature: 57192

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKFIRKLLGQDETEQTSPATAPADEAAPATPRAPKGARGGGAKKPRSNHEPTVVPASV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
HGINPALISKNAVRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLF
CCCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAAAAEPPKRLKRDELDRRTHAVDA
HHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
SGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
CCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCHHHH
FREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALA
HHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHH
CLTQLRKEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWH
HHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
DMRQRFEQYTAEGEIPVPALHRAMDDVLDMQTEKLAIHKRYSADMREIWGLQLRLEKRSG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCC
RSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGTKEKS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
PRKRRRRGGVRNRKPGEGAAEQAPDASDGSDD
HHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC
>Mature Secondary Structure
MIKKFIRKLLGQDETEQTSPATAPADEAAPATPRAPKGARGGGAKKPRSNHEPTVVPASV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
HGINPALISKNAVRVTDTLQQAGFRAFIVGGAVRDLLLGIAPKDFDVATDATPTEVQRLF
CCCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
RRARLIGRRFQIVHVQFGQELIEVSTFRALVDAPPEAAAAEPPKRLKRDELDRRTHAVDA
HHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
SGRVLRDNVWGEQHEDAARRDFTINAMYYDPSTQTVLDYHDGMADVRARLLRMIGDPATR
CCCEEECCCCCCCHHHHHHCCEEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCHHHH
FREDPVRMLRVVRFAAKLGFEIEPHTREPINALADLINNVPAARLFDEMLKLLLSGHALA
HHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHH
CLTQLRKEGLHHGLLPLLDVVLEQPQGEKFITLALNNTDARVRAGKPVSPGFLFATLLWH
HHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHH
DMRQRFEQYTAEGEIPVPALHRAMDDVLDMQTEKLAIHKRYSADMREIWGLQLRLEKRSG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCC
RSAMRLLEHQRFRAGYDFLLLRCESGELDAEVGQWWTDFIEGDAAAREALLTQGGTKEKS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
PRKRRRRGGVRNRKPGEGAAEQAPDASDGSDD
HHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]