Click here to switch to the map view.

The map label for this gene is yafV [C]

Identifier: 78065304

GI number: 78065304

Start: 729465

End: 730292

Strand: Reverse

Name: yafV [C]

Synonym: Bcep18194_A3828

Alternate gene names: 78065304

Gene position: 730292-729465 (Counterclockwise)

Preceding gene: 78065305

Following gene: 78065303

Centisome position: 19.77

GC content: 68.96

Gene sequence:

>828_bases
ATGACCGACCACACCCGTTCCGCCACGCCCTTCCAGGTCGCCGCGCTGCAGATGGTGAGCACGCCGGACGTCGCGCGCAA
TCTCGCCGAAGCCCGCCGCCTGATCGCGGAAGCCGCCGGCGAAGGCGCGCAGCTCGTGCTGCTGCCCGAGTATTTCTGCT
TCATGGGCCACCGCGACACCGACAAGCTCGCGCTCGCCGAACCCTACCAGGACGGCCCGATCCAGCACTTCCTCGCGGAC
GCCGCACGTCGCCACGGCATCTGGGTGATCGGCGGCACGCTACCGTTGAAGGCGCCGGAGCCCGATCGCGTGCTCAACAC
GACGCTCGTGTTCGATCCGTCCGGCAACGAAGCCGCGCGCTACGACAAGATCCACCTGTTCAACTTCGAGAAAGGCGACG
AGTCGTTCGACGAGGCGCGCACGATTCGCGCGGGCGATACGGTCGTCGCGTTCGACGCGCCGTTCGGGCGGGTCGGCCTG
TCGGTCTGTTACGATCTCCGCTTTCCGGAGCTGTATCGCAGGCTGGGTGACTGCGCGCTGATCGTCGTCCCGTCGGCGTT
TACGTATACGACGGGCCGCGCGCACTGGGAAACGCTGTTGCGCGCGCGGGCCGTCGAGAACCAGTGTTACGTGCTCGCGG
CCGCGCAAGGCGGCAAGCACGAGAACGGCCGGCGCACCTGGGGCCACAGCATGCTGATCGACCCGTGGGGCGAGATAGTC
GCGGTCCGCGACGTGGGCGCCAGCGTCGTGCTCGGCGCGATCGATCCGCAGCGCATCGCCGACGTGCGCCAGAGCCTGCC
CGCGTGGCGGCACCGCGTGCTCACCTGA

Upstream 100 bases:

>100_bases
GATGAATCACGGTCCGGCCGATGCGGCGAACCATTGAGCCACATGCCGAACCGCGCACGGTGCCGGGCCGCACCCCATTT
ATGACGCAACCGATCCCGCG

Downstream 100 bases:

>100_bases
TTCCGGCCGCGCCGCTTGAAACGCCGCGCGGCCAACCCATCTGCCCAGAAGCACCCGACAACTTTTTGAGAAACCCCGAT
ATCCGCATGAACATCATCGA

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTDHTRSATPFQVAALQMVSTPDVARNLAEARRLIAEAAGEGAQLVLLPEYFCFMGHRDTDKLALAEPYQDGPIQHFLAD
AARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAARYDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGRVGL
SVCYDLRFPELYRRLGDCALIVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
AVRDVGASVVLGAIDPQRIADVRQSLPAWRHRVLT

Sequences:

>Translated_275_residues
MTDHTRSATPFQVAALQMVSTPDVARNLAEARRLIAEAAGEGAQLVLLPEYFCFMGHRDTDKLALAEPYQDGPIQHFLAD
AARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAARYDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGRVGL
SVCYDLRFPELYRRLGDCALIVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
AVRDVGASVVLGAIDPQRIADVRQSLPAWRHRVLT
>Mature_274_residues
TDHTRSATPFQVAALQMVSTPDVARNLAEARRLIAEAAGEGAQLVLLPEYFCFMGHRDTDKLALAEPYQDGPIQHFLADA
ARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAARYDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGRVGLS
VCYDLRFPELYRRLGDCALIVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIVA
VRDVGASVVLGAIDPQRIADVRQSLPAWRHRVLT

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=268, Percent_Identity=37.6865671641791, Blast_Score=188, Evalue=4e-48,
Organism=Homo sapiens, GI297632350, Length=267, Percent_Identity=37.8277153558052, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI297632348, Length=267, Percent_Identity=37.8277153558052, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI5031947, Length=267, Percent_Identity=37.8277153558052, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786412, Length=230, Percent_Identity=30.8695652173913, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556280, Length=265, Percent_Identity=40.7547169811321, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6323383, Length=273, Percent_Identity=34.4322344322344, Blast_Score=168, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6322335, Length=295, Percent_Identity=33.2203389830508, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17933642, Length=278, Percent_Identity=35.6115107913669, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI21355835, Length=276, Percent_Identity=32.9710144927536, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30448; Mature: 30317

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHTRSATPFQVAALQMVSTPDVARNLAEARRLIAEAAGEGAQLVLLPEYFCFMGHRDT
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCC
DKLALAEPYQDGPIQHFLADAARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAAR
CCEEEECCCCCCHHHHHHHHHHHHCCEEEEECEEECCCCCCCCEEEEEEEECCCCCCCCC
YDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGRVGLSVCYDLRFPELYRRLGDCAL
CCEEEEEECCCCCCCHHHHHEEECCCEEEEECCCCCHHHHHHHEECCCHHHHHHHCCEEE
IVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
EEECCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEE
AVRDVGASVVLGAIDPQRIADVRQSLPAWRHRVLT
EEEECCCEEEEECCCHHHHHHHHHHCHHHHHHCCC
>Mature Secondary Structure 
TDHTRSATPFQVAALQMVSTPDVARNLAEARRLIAEAAGEGAQLVLLPEYFCFMGHRDT
CCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCC
DKLALAEPYQDGPIQHFLADAARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAAR
CCEEEECCCCCCHHHHHHHHHHHHCCEEEEECEEECCCCCCCCEEEEEEEECCCCCCCCC
YDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGRVGLSVCYDLRFPELYRRLGDCAL
CCEEEEEECCCCCCCHHHHHEEECCCEEEEECCCCCHHHHHHHEECCCHHHHHHHCCEEE
IVVPSAFTYTTGRAHWETLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
EEECCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEE
AVRDVGASVVLGAIDPQRIADVRQSLPAWRHRVLT
EEEECCCEEEEECCCHHHHHHHHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]