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The map label for this gene is rlmL [H]

Identifier: 78065271

GI number: 78065271

Start: 689248

End: 690549

Strand: Reverse

Name: rlmL [H]

Synonym: Bcep18194_A3795

Alternate gene names: 78065271

Gene position: 690549-689248 (Counterclockwise)

Preceding gene: 78065272

Following gene: 78065268

Centisome position: 18.69

GC content: 71.66

Gene sequence:

>1302_bases
ATGTCCTCGCCCACCCTGTACGAATTCTTCGCCCCCTGCCCGCGCGGCCTCGAAGCGGCGCTTGCCGCCGAGCTGGCCGA
AATCGCCGGCCGCCACCTGAACGGCGCGCCGTTCACCGCGGGCGCGCAGGTGCCGGGCGGCGTCCACTTCAGCGGCGGCT
GGGCCGCCGGCATGGCCGCGAACCTCCATTCGCGGATCGCGAGCCGGGTCCTGCTGAAGATCGCGCACCGCGCGTACCGC
AACGAGCAGGACGTCTACGCGCTCGCGCTCGAGCAGCCGTGGGAGCGCTGGTTCGCGGCCACGCAGACGCTGCGCGTCGA
CATCACCGCGATCAAGTCGCCGCTGAAGAGCCTCGAATTCGCGACGTTGCGCGTGAAGGACGCGATCTGCGACCGGATGC
GCGACAAGACCGGCGCGCGCCCGAGCATCGACACCGGTGCGCCGGACGTGCGCGTGTTCGCCTTCCTGACGGCGGGCGAA
TGCACGCTGTACCTCGACACGTCGGGCGAGCCGCTGTTCAAGCGCGGCTGGCGTCTCGACAAGGGCGCGGCGCCGCTGCG
CGAGAACCTCGCGGCCGGCATCCTGCGCCTGACGGGCTGGACGCCCGGCACGGCACTGTACGACCCGATGTGCGGCAGCG
GCACGTTCCTCGCGGAAGCCGCGCAGATCGCGCTCGGCGTGGCGCCGGGTGTCGAGCGCCGGTTCGGCTTCGAGAAACTC
AAGCAATACGACATCACCGCGTGGCAGGGGCTGAAGGTCCCGGCGCTGGACGCGAAGCGCGCGGCGCGCGGCAAACGCGG
CGACGCACTCGGCGTATACGGCAGCGACATCTCCGGCGACATGCTCGAGAAGGCGCGCGCGAACCTCGACCGCGCAGGCG
TGCCGTCGGTATGGCTCAAGCAGGTCGACGCACGCGGGATGACGCCGCCGTGCGACGGCCCGGGCATCATCCTCGCGAAC
CCGCCGTACGGCGAGCGGATCGAAGTGCGCGGCCGCAGCGCGCGCGGCGAGGTGCGCGAGACGGGCCGCAACCGCGGCAA
CGACGATGCATTCCGCCGCACCCACACCGACGCGCCGGACAGCGAGTTCTTCAACGCGCTCGGCGATGCGCTGAAGCAGC
GCTTCACGGGCTGGCAGGCGTTCCTGCTGACGTCCGACCGATCGCTGCCGGGCCAGCTGCGGCTGCGCGAATCGGCGAAG
ACGCCGCTGTTCAACGGCGCGCTCGAATGCCGGCTGTTCCGCTTCGACCTGATCGCCGGCAGCGTGAAGGCGCGCCCGGC
CACGCCGGAAGGCGACGCGTAA

Upstream 100 bases:

>100_bases
CGCCCGGGCCCGCGCCGCAGCATCGAAACGCGACGCAACGCCGGCCCCGGCCGCCCGCGCCGCGTACAATAGTGGACTTT
TGCACGTCCTCACCCGCCTT

Downstream 100 bases:

>100_bases
CCTGCGTCATGGTCGCGATGGGGCCGCTGGTGCGCCCCCTCCGCGGCAACCGGAACGCCGCCGTTACCGCACCCGCGGCA
ACGGCGGCGTTTCGTTTCAG

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSSPTLYEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAANLHSRIASRVLLKIAHRAYR
NEQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEFATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTAGE
CTLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKL
KQYDITAWQGLKVPALDAKRAARGKRGDALGVYGSDISGDMLEKARANLDRAGVPSVWLKQVDARGMTPPCDGPGIILAN
PPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDSEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAK
TPLFNGALECRLFRFDLIAGSVKARPATPEGDA

Sequences:

>Translated_433_residues
MSSPTLYEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAANLHSRIASRVLLKIAHRAYR
NEQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEFATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTAGE
CTLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKL
KQYDITAWQGLKVPALDAKRAARGKRGDALGVYGSDISGDMLEKARANLDRAGVPSVWLKQVDARGMTPPCDGPGIILAN
PPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDSEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAK
TPLFNGALECRLFRFDLIAGSVKARPATPEGDA
>Mature_432_residues
SSPTLYEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAANLHSRIASRVLLKIAHRAYRN
EQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEFATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTAGEC
TLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKLK
QYDITAWQGLKVPALDAKRAARGKRGDALGVYGSDISGDMLEKARANLDRAGVPSVWLKQVDARGMTPPCDGPGIILANP
PYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPDSEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAKT
PLFNGALECRLFRFDLIAGSVKARPATPEGDA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=393, Percent_Identity=33.0788804071247, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 46970; Mature: 46839

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPTLYEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAA
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHH
NLHSRIASRVLLKIAHRAYRNEQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEF
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHH
ATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTAGECTLYLDTSGEPLFKRGWRLD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHCCCCCC
KGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCHHHH
KQYDITAWQGLKVPALDAKRAARGKRGDALGVYGSDISGDMLEKARANLDRAGVPSVWLK
HHHCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHH
QVDARGMTPPCDGPGIILANPPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPD
HHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHCCCCCCH
SEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAKTPLFNGALECRLFRFDLIAG
HHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEHHHCCCCCCCCCEEEEEEEEEEHCC
SVKARPATPEGDA
CCCCCCCCCCCCC
>Mature Secondary Structure 
SSPTLYEFFAPCPRGLEAALAAELAEIAGRHLNGAPFTAGAQVPGGVHFSGGWAAGMAA
CCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHH
NLHSRIASRVLLKIAHRAYRNEQDVYALALEQPWERWFAATQTLRVDITAIKSPLKSLEF
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHH
ATLRVKDAICDRMRDKTGARPSIDTGAPDVRVFAFLTAGECTLYLDTSGEPLFKRGWRLD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCHHHHCCCCCC
KGAAPLRENLAAGILRLTGWTPGTALYDPMCGSGTFLAEAAQIALGVAPGVERRFGFEKL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCHHHH
KQYDITAWQGLKVPALDAKRAARGKRGDALGVYGSDISGDMLEKARANLDRAGVPSVWLK
HHHCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHH
QVDARGMTPPCDGPGIILANPPYGERIEVRGRSARGEVRETGRNRGNDDAFRRTHTDAPD
HHHCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCHHHHHCCCCCCCHHHHHCCCCCCH
SEFFNALGDALKQRFTGWQAFLLTSDRSLPGQLRLRESAKTPLFNGALECRLFRFDLIAG
HHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEHHHCCCCCCCCCEEEEEEEEEEHCC
SVKARPATPEGDA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA