Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 78065004

GI number: 78065004

Start: 393426

End: 394067

Strand: Direct

Name: hisH

Synonym: Bcep18194_A3527

Alternate gene names: 78065004

Gene position: 393426-394067 (Clockwise)

Preceding gene: 78065003

Following gene: 78065005

Centisome position: 10.65

GC content: 66.67

Gene sequence:

>642_bases
ATGAAAACTTCGATTGCGATTGTGGATTATGGGATGGGCAACCTGCGCTCGGTCGCGCAGGCGCTCAAGAAGGCCGAACC
GGCCGCCGACGTGGCGATCGTCGACACGCCGGCCGCGATCCGCGCGGCCGACCGTGTCGTGCTGCCCGGCCAGGGCGCGA
TGCCCGACTGCATGCGCAGCCTCGGCGAATCGGGCCTGCAGGAGGCCGTGATCGAGGCGTCGCGCACGAAGCCGCTGCTC
GGCGTGTGCGTCGGCGAGCAGATGCTGTTCGACTGGAGCGCGGAAGGCGACACGAAGGGCCTCGGCCTGTTGCCCGGCAA
GGTCGTGCGTTTCGAGCTCGACGGCCGCCTGCAGGACGACGGTTCGCGCTTCAAGGTGCCGCAGATGGGCTGGAACCGCG
TGCGCCAGTCGCAGCCGCACCCGCTGTGGGACGGCGTGCCCGACGACGCGTATTTCTACTTTGTGCACAGCTATTACGTG
ACGCCGGACAACCCGGCTCATACGGTGGGCGAAACGGCTTACGGCGCGCCGTTTACGTCCGCGGTCGCGCGGGATAACCT
CTTCGCGACCCAATTCCACCCCGAGAAAAGCGCGGAGGTCGGGTTGCGTCTGTATCGCAACTTCGTACACTGGAAGCCGT
GA

Upstream 100 bases:

>100_bases
CGGGCATCAACATCGCCACGCGGCTGATGGGTCTGATGCTGTCGGCGCTGGCGGTGGAATTCATCGTCAATGGACTGAGG
GCGCTACTGCCTGCACTGAG

Downstream 100 bases:

>100_bases
ACGTCGCGTGCGTGCCACAGTCGGCGGGCCGGAAAGGCCTGTCGCATGGGGCTCGCGCGCCTAAGCGCCGAAAGAGTTGT
ACTAAACTAGCGAGACGGCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRSLGESGLQEAVIEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDDGSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYV
TPDNPAHTVGETAYGAPFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRSLGESGLQEAVIEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDDGSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYV
TPDNPAHTVGETAYGAPFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRSLGESGLQEAVIEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDDGSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYV
TPDNPAHTVGETAYGAPFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=36.5384615384615, Blast_Score=122, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=32.1100917431193, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23350; Mature: 23350

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRS
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEAVIEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDD
HHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEEECCEECCC
GSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYVTPDNPAHTVGETAYGAPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHCCCCCCCCCHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRS
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEAVIEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDD
HHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEEECCEECCC
GSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYVTPDNPAHTVGETAYGAPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHCCCCCCCCCHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]