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The map label for this gene is thiE [H]

Identifier: 78064988

GI number: 78064988

Start: 379969

End: 381093

Strand: Direct

Name: thiE [H]

Synonym: Bcep18194_A3511

Alternate gene names: 78064988

Gene position: 379969-381093 (Clockwise)

Preceding gene: 78064987

Following gene: 78064989

Centisome position: 10.29

GC content: 70.76

Gene sequence:

>1125_bases
ATGAGCGCGCGTTTCGCCGATGCATTCTGGCCGCCGGCCGACGAACTGGCCGAAGCGGCCGAACGGATTCGCGCGCGGCT
CGGCGACTGGCCGGACGGTGCGGCGCCGTGGCGGCTTTGCGTTGCGGTGCCCGATGTGCCGGCCGATGGCGATGTGCTGA
TCGTCTCGTCCGGCGACCGTGCCGCGCAGGCGCGCGCGTCGGCTGCGTCGCGGCCCGCTGCACCGGGCGCGGTCGCGATC
GAATTCGACGAGCAGGGCGCAGTGCTGCATACGGCGGGCGCGCGCTACGCGCTCGCCGCCGCGCATCCGCTCGGGGACGA
CTGGATCGCGGCGCTTGCCGCATTCCTCGATTGCGGCTTTGCGCCGGTCGATGCGCTGGTGCTGGCGCTGGCCTGGCGCG
ACGGCGATGAAACGCGCGGCGCCGACGCGTGGCCGGTCGACGCCACGCGGTTCCCGCGGATCGCCGGGCTGCCGGCCGCG
CCCGAACCCGCGTTCGCGCCGTGCCCCGAGCAGCTTGGCCTTTATCCAGTCGTGCCGGATGCCGAATGGGTCGAGCGCGT
GCTCGATTGCGGCGTGCTGACCGTGCAACTACGTGTGAAAGGCGCGACGCCGGACACCTTGCGGCGGGAAATCGCCCGCG
CGGTCGCGGCTGGGCGCCGCCATCCCGATGCGCGTGTGTTCATCAACGATCACTGGCAGATCGCTGCAGATGAAGGTGCG
TATGGTGTTCACCTCGGTCAGGAAGACCTCGAAACGGCCGATCTGGCCGCGATTGCGCGCGCGGGCTTGCGGCTCGGACT
TTCGAGCCACGGTTATTACGAAATGTTGCGGGCGCTGCATGAGCGCCCGAGCTACCTCGCGCTCGGCCCCGTGTACGCGA
CCGCGACCAAGGCCGTTGCTGCGCCGCCACAGGGCCTCGCCCGGATCGCCCGTTATGCGCACTTCGCGGGCGCCCAGGCG
CCGCTCGTCGCGATCGGCGGGGTGGGGCTCGACGCGCTGCCGGCCGTGCTGGCAACGGGCGTCGGCAGTGTCGCGGTGGT
CAGTGCGGTGACGGGCGCGACCGACTATCGGGCTGCCGTTGCTGCGCTGCAGCAATGTTTTCCCGGACAATTTGACAATC
ATTGA

Upstream 100 bases:

>100_bases
GTGCTGCCTATCTCGCCGGGCCGATGGATGCACGCGAGACCGCGCATGCGAGCACGCCGGTCGTCGGCATGCCGTTCTGG
CACCAGGACGGGGGCGGCGC

Downstream 100 bases:

>100_bases
CCGCAGGGGCTGAAGCGGCGTCACGACATCATTCCAAGGCAGGCCCTATAATTCGGCGTTCTGCGTAAAAGGACTGTCCG
CTCCGTGAGCCCCACTCCTA

Product: thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSARFADAFWPPADELAEAAERIRARLGDWPDGAAPWRLCVAVPDVPADGDVLIVSSGDRAAQARASAASRPAAPGAVAI
EFDEQGAVLHTAGARYALAAAHPLGDDWIAALAAFLDCGFAPVDALVLALAWRDGDETRGADAWPVDATRFPRIAGLPAA
PEPAFAPCPEQLGLYPVVPDAEWVERVLDCGVLTVQLRVKGATPDTLRREIARAVAAGRRHPDARVFINDHWQIAADEGA
YGVHLGQEDLETADLAAIARAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVAAPPQGLARIARYAHFAGAQA
PLVAIGGVGLDALPAVLATGVGSVAVVSAVTGATDYRAAVAALQQCFPGQFDNH

Sequences:

>Translated_374_residues
MSARFADAFWPPADELAEAAERIRARLGDWPDGAAPWRLCVAVPDVPADGDVLIVSSGDRAAQARASAASRPAAPGAVAI
EFDEQGAVLHTAGARYALAAAHPLGDDWIAALAAFLDCGFAPVDALVLALAWRDGDETRGADAWPVDATRFPRIAGLPAA
PEPAFAPCPEQLGLYPVVPDAEWVERVLDCGVLTVQLRVKGATPDTLRREIARAVAAGRRHPDARVFINDHWQIAADEGA
YGVHLGQEDLETADLAAIARAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVAAPPQGLARIARYAHFAGAQA
PLVAIGGVGLDALPAVLATGVGSVAVVSAVTGATDYRAAVAALQQCFPGQFDNH
>Mature_373_residues
SARFADAFWPPADELAEAAERIRARLGDWPDGAAPWRLCVAVPDVPADGDVLIVSSGDRAAQARASAASRPAAPGAVAIE
FDEQGAVLHTAGARYALAAAHPLGDDWIAALAAFLDCGFAPVDALVLALAWRDGDETRGADAWPVDATRFPRIAGLPAAP
EPAFAPCPEQLGLYPVVPDAEWVERVLDCGVLTVQLRVKGATPDTLRREIARAVAAGRRHPDARVFINDHWQIAADEGAY
GVHLGQEDLETADLAAIARAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVAAPPQGLARIARYAHFAGAQAP
LVAIGGVGLDALPAVLATGVGSVAVVSAVTGATDYRAAVAALQQCFPGQFDNH

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=205, Percent_Identity=52.1951219512195, Blast_Score=198, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 39041; Mature: 38910

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARFADAFWPPADELAEAAERIRARLGDWPDGAAPWRLCVAVPDVPADGDVLIVSSGDR
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCH
AAQARASAASRPAAPGAVAIEFDEQGAVLHTAGARYALAAAHPLGDDWIAALAAFLDCGF
HHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCC
APVDALVLALAWRDGDETRGADAWPVDATRFPRIAGLPAAPEPAFAPCPEQLGLYPVVPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHCCCEECCCC
AEWVERVLDCGVLTVQLRVKGATPDTLRREIARAVAAGRRHPDARVFINDHWQIAADEGA
HHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCC
YGVHLGQEDLETADLAAIARAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVA
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHC
APPQGLARIARYAHFAGAQAPLVAIGGVGLDALPAVLATGVGSVAVVSAVTGATDYRAAV
CCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
AALQQCFPGQFDNH
HHHHHHCCCCCCCC
>Mature Secondary Structure 
SARFADAFWPPADELAEAAERIRARLGDWPDGAAPWRLCVAVPDVPADGDVLIVSSGDR
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCH
AAQARASAASRPAAPGAVAIEFDEQGAVLHTAGARYALAAAHPLGDDWIAALAAFLDCGF
HHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCC
APVDALVLALAWRDGDETRGADAWPVDATRFPRIAGLPAAPEPAFAPCPEQLGLYPVVPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHCCCEECCCC
AEWVERVLDCGVLTVQLRVKGATPDTLRREIARAVAAGRRHPDARVFINDHWQIAADEGA
HHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCC
YGVHLGQEDLETADLAAIARAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVA
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHC
APPQGLARIARYAHFAGAQAPLVAIGGVGLDALPAVLATGVGSVAVVSAVTGATDYRAAV
CCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
AALQQCFPGQFDNH
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA