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The map label for this gene is rhtB [H]

Identifier: 78064884

GI number: 78064884

Start: 265227

End: 265868

Strand: Direct

Name: rhtB [H]

Synonym: Bcep18194_A3407

Alternate gene names: 78064884

Gene position: 265227-265868 (Clockwise)

Preceding gene: 78064883

Following gene: 78064886

Centisome position: 7.18

GC content: 67.6

Gene sequence:

>642_bases
ATGAACTTGCATACGTGGTGGCTTTTCGTGGCGACGGTGTTCGTCGTGTCGGCGATTCCGGGCCCGAACATGCTGCTGGT
GATGACGCACGGCGCGCGGCACGGGCTGCGGCGTTCGACGTGGACGATGGCCGGTTGCCTCACCGCGCTGGTGCTGATGC
TGTCGGTGTCGGCGGCGGGCCTCGGCGCGGTGCTCGAAGCCTGGCCGGCGATGTTCAACACGTTGCGCTTCGCGGGTGCG
GCCTACCTGATCTACCTCGGTGTGAAGGCGTGGCGTGCACGCGTCGATGACGAGCAGCCGGCCGCCGACGTCGACGCCGT
GTCGCGCCAGGCCGCACCGGCATCGCGCGGGACGCTGTTCCGCAACGGTTTCCTGGTCGCGGGCAGCAACCCGAAGGCGA
TCCTGTTCGCGGCCGCGCTGTTGCCGCAGTTCATCAACGCGGCCGAGCCGACGCTGCCGCAGTTCGGCATGCTCGTCGTC
ACGTTCGCGGTGATCGAGGTGAGCTGGTATTTCGTCTACGCGTCGTTCGGCACGCGCATCGGCGCGACGTTGAAGAGCCA
GAGCGTGGCGAAGATCTTCAACCGGCTGACGGGCGGGCTGTTCGTCGGCTTCGGCGCGATGATGGCGCTGGTCCGTCACT
GA

Upstream 100 bases:

>100_bases
GCGCTGCGCCGCGCGGGCCGCTGATCCGCCGCGCGACAGAAGTCCGGCGCACCGGCATAATCGACGTTTCGTTTTCCCTT
ACATCGACGCAGAGGCGGGC

Downstream 100 bases:

>100_bases
TTTCGCACTGACCCCGGTGCGCCGAACGCCCCGTCCCTCGTAAGAGGGCGGGGCGTTCGGCTTTTCGGGCCTGCGTCACG
CCGGCCTAGGCACGCGGGCG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTWTMAGCLTALVLMLSVSAAGLGAVLEAWPAMFNTLRFAGA
AYLIYLGVKAWRARVDDEQPAADVDAVSRQAAPASRGTLFRNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVV
TFAVIEVSWYFVYASFGTRIGATLKSQSVAKIFNRLTGGLFVGFGAMMALVRH

Sequences:

>Translated_213_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTWTMAGCLTALVLMLSVSAAGLGAVLEAWPAMFNTLRFAGA
AYLIYLGVKAWRARVDDEQPAADVDAVSRQAAPASRGTLFRNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVV
TFAVIEVSWYFVYASFGTRIGATLKSQSVAKIFNRLTGGLFVGFGAMMALVRH
>Mature_213_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTWTMAGCLTALVLMLSVSAAGLGAVLEAWPAMFNTLRFAGA
AYLIYLGVKAWRARVDDEQPAADVDAVSRQAAPASRGTLFRNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVV
TFAVIEVSWYFVYASFGTRIGATLKSQSVAKIFNRLTGGLFVGFGAMMALVRH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=213, Percent_Identity=35.6807511737089, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1788099, Length=216, Percent_Identity=29.6296296296296, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22924; Mature: 22924

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTWTMAGCLTALVLMLSVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH
LGAVLEAWPAMFNTLRFAGAAYLIYLGVKAWRARVDDEQPAADVDAVSRQAAPASRGTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEE
RNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVVTFAVIEVSWYFVYASFGTRI
ECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHH
GATLKSQSVAKIFNRLTGGLFVGFGAMMALVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTWTMAGCLTALVLMLSVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH
LGAVLEAWPAMFNTLRFAGAAYLIYLGVKAWRARVDDEQPAADVDAVSRQAAPASRGTLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEE
RNGFLVAGSNPKAILFAAALLPQFINAAEPTLPQFGMLVVTFAVIEVSWYFVYASFGTRI
ECCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHH
GATLKSQSVAKIFNRLTGGLFVGFGAMMALVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]