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The map label for this gene is metF [H]

Identifier: 78064854

GI number: 78064854

Start: 235886

End: 236716

Strand: Direct

Name: metF [H]

Synonym: Bcep18194_A3377

Alternate gene names: 78064854

Gene position: 235886-236716 (Clockwise)

Preceding gene: 78064853

Following gene: 78064857

Centisome position: 6.39

GC content: 64.98

Gene sequence:

>831_bases
ATGAAACCGATCGAACTCTCGTTTGAATTCTTCCCGCCGAAAACGGCGGAGGGCGTCGAGAAGCTGCGCGCCACGCGTGC
GCAGCTGCTGCCGCTGAAGCCGAAATTCGTTTCCTGCACGTTCGGCGCCGGCGGTTCGACGCAGCAGGGTACGCTCGATA
CGGTGCTCGACATGCAGAAGGACGGCCTCGAGGCCGCACCGCACCTGTCGTGCATCGGTTCGTCGCGTGACAGCCTGCGC
GCAATCCTCGACCAGTACCGCTCGTACGGCATCCGTCACATCGTCGCGCTGCGTGGCGACCTGCCGTCGGGGATGGGCGA
AGTCGGCGAACTGCGTTATGCGTCGGAACTCGTCAGCTTCATCCGTGCCGAGCATGGCGACTGGTTCCACATCGAAGTCG
CCGGCTATCCGGAGTACCACCCGCAAGCGCGCTCGCCGAAGGCCGACCTCGAGAATTTCGCGCGCAAGGTGAAAGCCGGC
GCGAATTCCGCGATCACGCAGTACTTCTTCAACGCCGATGCGTATTTCCGCTTCGTCGACGATGCGCGCAAGCTGGGCGT
GGACGTGCCGATCGTGCCGGGCATCATGCCGATCACGAACTTCTCGCAGCTGATGCGCTTCTCCGAGATGTGCGGCGCCG
AAGTGCCGCGCTGGGTCGCGCGCCGGCTCGAGAGCTTCGGCGACGATCGCGAGTCGATCCGTGCGTTCGGCGCGGACGTC
GTCACGAGCCTGTGCCAGCGTCTGGTCGACGCGGGCGTGCCGGGCCTGCACTTCTATACGCTGAACACGGCGGCCGCGAC
GCGGACGATCTGTGAACGGCTCGTCGTGTAA

Upstream 100 bases:

>100_bases
CTGGTCCGCGTTCTTCGGTTCGATCCTGTACAGCATCGTGTCGTGGCTGCTGTCCGCGCTGATCTTCGGTCAGCGCGACA
TCGGCTGACCTCTTCGAATC

Downstream 100 bases:

>100_bases
GCGCGCTCACGCGATGGCATGCGTAAAAGCCCCGCCGGTGATGCCGGCGGGGCTTTTTTTTCGAGACCTCCGGAGCGCGG
CCGCGCGCGTCAGCGCTGCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKPIELSFEFFPPKTAEGVEKLRATRAQLLPLKPKFVSCTFGAGGSTQQGTLDTVLDMQKDGLEAAPHLSCIGSSRDSLR
AILDQYRSYGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFHIEVAGYPEYHPQARSPKADLENFARKVKAG
ANSAITQYFFNADAYFRFVDDARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
VTSLCQRLVDAGVPGLHFYTLNTAAATRTICERLVV

Sequences:

>Translated_276_residues
MKPIELSFEFFPPKTAEGVEKLRATRAQLLPLKPKFVSCTFGAGGSTQQGTLDTVLDMQKDGLEAAPHLSCIGSSRDSLR
AILDQYRSYGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFHIEVAGYPEYHPQARSPKADLENFARKVKAG
ANSAITQYFFNADAYFRFVDDARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
VTSLCQRLVDAGVPGLHFYTLNTAAATRTICERLVV
>Mature_276_residues
MKPIELSFEFFPPKTAEGVEKLRATRAQLLPLKPKFVSCTFGAGGSTQQGTLDTVLDMQKDGLEAAPHLSCIGSSRDSLR
AILDQYRSYGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFHIEVAGYPEYHPQARSPKADLENFARKVKAG
ANSAITQYFFNADAYFRFVDDARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
VTSLCQRLVDAGVPGLHFYTLNTAAATRTICERLVV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=278, Percent_Identity=39.568345323741, Blast_Score=191, Evalue=5e-49,
Organism=Escherichia coli, GI1790377, Length=274, Percent_Identity=46.7153284671533, Blast_Score=246, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI25147441, Length=280, Percent_Identity=34.2857142857143, Blast_Score=174, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI25147436, Length=280, Percent_Identity=34.2857142857143, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6321313, Length=284, Percent_Identity=36.2676056338028, Blast_Score=174, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325234, Length=286, Percent_Identity=32.1678321678322, Blast_Score=152, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24662658, Length=268, Percent_Identity=26.865671641791, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30555; Mature: 30555

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIELSFEFFPPKTAEGVEKLRATRAQLLPLKPKFVSCTFGAGGSTQQGTLDTVLDMQK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
DGLEAAPHLSCIGSSRDSLRAILDQYRSYGIRHIVALRGDLPSGMGEVGELRYASELVSF
CCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
IRAEHGDWFHIEVAGYPEYHPQARSPKADLENFARKVKAGANSAITQYFFNADAYFRFVD
HHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VTSLCQRLVDAGVPGLHFYTLNTAAATRTICERLVV
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPIELSFEFFPPKTAEGVEKLRATRAQLLPLKPKFVSCTFGAGGSTQQGTLDTVLDMQK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
DGLEAAPHLSCIGSSRDSLRAILDQYRSYGIRHIVALRGDLPSGMGEVGELRYASELVSF
CCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
IRAEHGDWFHIEVAGYPEYHPQARSPKADLENFARKVKAGANSAITQYFFNADAYFRFVD
HHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VTSLCQRLVDAGVPGLHFYTLNTAAATRTICERLVV
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]