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The map label for this gene is stp [H]

Identifier: 78064789

GI number: 78064789

Start: 155813

End: 157426

Strand: Direct

Name: stp [H]

Synonym: Bcep18194_A3312

Alternate gene names: 78064789

Gene position: 155813-157426 (Clockwise)

Preceding gene: 78064787

Following gene: 78064791

Centisome position: 4.22

GC content: 71.19

Gene sequence:

>1614_bases
ATGGATCACCTTTTTTCAGCCGCTTCGGACCTGTCGACACGCCGGCGTGCGTGGGTGCTGGCCGCCGTCTGCATGGCGGC
CGTCGCGCTGCCGCTGTCGTTTTCGGGCGGCGCGGTCGCGACGCCCGCGATCGGCCGTGACCTGCACGGCGGGACGGTCG
CGATGAACTGGATCACCAACGCGTTCATGCTCGCGTTCGGCAGCTTCCTGATGGCGGCCGGCGCGCTGGCCGACCAGTTC
GGCCGCAAGCGCCTGTTCGCGATCGGCGTTGGCGGCTTCACGCTGATGTCGGTCGCGCTCGCGTTCGCGCCGTCGATGCT
CGCGGTTGACCTGCTGCGCGCCGCGCAGGGGCTCGCGGCGGCGGCGGCGCTCGCGGGCGGCACAGCCGCGCTCGCGCAGG
AGTTCGACGGCGCGGCGCGCACGCGCGCGTTCAGCCTGCTCGGCACGACTTTCGGGATCGGGCTCGCGTTCGGGCCCGTG
CTGGCCGGTGCGCTGATCGGGCATTACGGCTGGCGCGCGATCTTCGTGACGAGCGCGGTGGCCGGCGGGCTGTCGCTGGT
ATTCGGGCTGCCGCGCATGCACGAGTCGCGCGACCCGCATGCGACGGGGCTCGACTGGCCCGGCACGGCCGCGTTCACCA
CCGCGCTGACGCTGTTCACATTCGGCGTGATCGAGGCACCCGCGCGCGGCGGGTCGAGCCCGCTGGTGATCGCGCTGCTG
GCAGGCGCGGCGCTCGGCGCGTGTGCGTTTGTCGCGATCGAGACGCGGGTCGCGCGGCCGATGCTCGACCTGTCGCTGTT
CCGGATCGCACGCTTCGTCGGCGTGCAGGTGCTGCCGGTGTCGACCTGCTGCTGCTACATCGTGCTGCTTGTCGTGCTGC
CGCTGCGCTTCATCGGCATCGACGGGTTCAGCGAGATCGACGCCGGCTGGCTGATGCTCGCGATTTCCGCACCGATGCTG
GTCGTGCCGTTTGTCGCGGCCACGCTCACGCGCTGGCTGTCGGCGGGCGTGATATCAGGGCTCGGGCTTCTGATCGCTGC
GGCCGGGCTCGTGTGGCTGGGTATCGCACTGCGCGGCGGTGCGGGGGCCGCGGCGATCGCGCCGATGGTGACGATCGGCG
TCGGGGCCGGCATGCCGTGGGGGCTGATGGACGGACTGTCGGTGAGCGTCGTGCCGAAGGAACGCGCGGGGATGGCGACG
GGGATTTTCAGCACGACGCGCGTGGCCGGCGAGGGGATCGCGCTCGCGATCGTTGGTGCAGTGCTGGCTGGGCTCGCGCG
GACGGGGTTGGCGCAAGCGGCTGGTGCTGGCGGTACGCCTGACGCGACGCTGCGTGCGGCGGCGCGGCTCGCAACCGGTG
ATCTCGCGGGTGCGGCGGATGCGTTGCCCGGGGTTGGACATGCGGTGCTGCTTGCGAGTTATACGCATGCGTTCGATCGG
TTGTTGATCGGGTTGGCAGTCGTGACGGTGCTGTGTGCGGGGGTGGTGTTTGCGTTTCTTGGTGGGCGGCCGGCGGCTAC
CGAAACGGGTGACGACTCCGACGAGCACGCGCATGCCGACGTGCCTGTCCGCAGCCGGATCGGAGCGGCTTGTGCGGAGA
CGCGCGGGCGTTGA

Upstream 100 bases:

>100_bases
CCGTTATATGCGGAGCTTTGCGTGGTTTTTCGCGGATTGAATCGGATTTATCTTCTGTCGCTCTGACGGCGTATCCGCGC
CGGCCTTTTGAGGAGCGATG

Downstream 100 bases:

>100_bases
GCCTCGCGTCGTCGCACACGGGCGCTCCGGCCACGGCGGCCGGAGCGCCGTCTTATTTCGTTGTCGCTGCATTCACACGC
CGGCGATCCGACTCGCCCAT

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MDHLFSAASDLSTRRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGGTVAMNWITNAFMLAFGSFLMAAGALADQF
GRKRLFAIGVGGFTLMSVALAFAPSMLAVDLLRAAQGLAAAAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAFGPV
LAGALIGHYGWRAIFVTSAVAGGLSLVFGLPRMHESRDPHATGLDWPGTAAFTTALTLFTFGVIEAPARGGSSPLVIALL
AGAALGACAFVAIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLAISAPML
VVPFVAATLTRWLSAGVISGLGLLIAAAGLVWLGIALRGGAGAAAIAPMVTIGVGAGMPWGLMDGLSVSVVPKERAGMAT
GIFSTTRVAGEGIALAIVGAVLAGLARTGLAQAAGAGGTPDATLRAAARLATGDLAGAADALPGVGHAVLLASYTHAFDR
LLIGLAVVTVLCAGVVFAFLGGRPAATETGDDSDEHAHADVPVRSRIGAACAETRGR

Sequences:

>Translated_537_residues
MDHLFSAASDLSTRRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGGTVAMNWITNAFMLAFGSFLMAAGALADQF
GRKRLFAIGVGGFTLMSVALAFAPSMLAVDLLRAAQGLAAAAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAFGPV
LAGALIGHYGWRAIFVTSAVAGGLSLVFGLPRMHESRDPHATGLDWPGTAAFTTALTLFTFGVIEAPARGGSSPLVIALL
AGAALGACAFVAIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLAISAPML
VVPFVAATLTRWLSAGVISGLGLLIAAAGLVWLGIALRGGAGAAAIAPMVTIGVGAGMPWGLMDGLSVSVVPKERAGMAT
GIFSTTRVAGEGIALAIVGAVLAGLARTGLAQAAGAGGTPDATLRAAARLATGDLAGAADALPGVGHAVLLASYTHAFDR
LLIGLAVVTVLCAGVVFAFLGGRPAATETGDDSDEHAHADVPVRSRIGAACAETRGR
>Mature_537_residues
MDHLFSAASDLSTRRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGGTVAMNWITNAFMLAFGSFLMAAGALADQF
GRKRLFAIGVGGFTLMSVALAFAPSMLAVDLLRAAQGLAAAAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAFGPV
LAGALIGHYGWRAIFVTSAVAGGLSLVFGLPRMHESRDPHATGLDWPGTAAFTTALTLFTFGVIEAPARGGSSPLVIALL
AGAALGACAFVAIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLAISAPML
VVPFVAATLTRWLSAGVISGLGLLIAAAGLVWLGIALRGGAGAAAIAPMVTIGVGAGMPWGLMDGLSVSVVPKERAGMAT
GIFSTTRVAGEGIALAIVGAVLAGLARTGLAQAAGAGGTPDATLRAAARLATGDLAGAADALPGVGHAVLLASYTHAFDR
LLIGLAVVTVLCAGVVFAFLGGRPAATETGDDSDEHAHADVPVRSRIGAACAETRGR

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=396, Percent_Identity=26.7676767676768, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788710, Length=301, Percent_Identity=25.9136212624585, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790195, Length=401, Percent_Identity=22.1945137157107, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=249, Percent_Identity=29.3172690763052, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54011; Mature: 54011

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLFSAASDLSTRRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGGTVAMNWITN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEHHHHHH
AFMLAFGSFLMAAGALADQFGRKRLFAIGVGGFTLMSVALAFAPSMLAVDLLRAAQGLAA
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAFGPVLAGALIGHYGWRAIFVTSAV
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
AGGLSLVFGLPRMHESRDPHATGLDWPGTAAFTTALTLFTFGVIEAPARGGSSPLVIALL
HHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AGAALGACAFVAIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRFIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHCC
DGFSEIDAGWLMLAISAPMLVVPFVAATLTRWLSAGVISGLGLLIAAAGLVWLGIALRGG
CCCCHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AGAAAIAPMVTIGVGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAIVGA
CCHHHHHHHHHHHCCCCCCCHHHCCCEEEEECHHHCCCHHHHHHHHHHCCCCHHHHHHHH
VLAGLARTGLAQAAGAGGTPDATLRAAARLATGDLAGAADALPGVGHAVLLASYTHAFDR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHH
LLIGLAVVTVLCAGVVFAFLGGRPAATETGDDSDEHAHADVPVRSRIGAACAETRGR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDHLFSAASDLSTRRRAWVLAAVCMAAVALPLSFSGGAVATPAIGRDLHGGTVAMNWITN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEHHHHHH
AFMLAFGSFLMAAGALADQFGRKRLFAIGVGGFTLMSVALAFAPSMLAVDLLRAAQGLAA
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAALAGGTAALAQEFDGAARTRAFSLLGTTFGIGLAFGPVLAGALIGHYGWRAIFVTSAV
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
AGGLSLVFGLPRMHESRDPHATGLDWPGTAAFTTALTLFTFGVIEAPARGGSSPLVIALL
HHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AGAALGACAFVAIETRVARPMLDLSLFRIARFVGVQVLPVSTCCCYIVLLVVLPLRFIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHCC
DGFSEIDAGWLMLAISAPMLVVPFVAATLTRWLSAGVISGLGLLIAAAGLVWLGIALRGG
CCCCHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AGAAAIAPMVTIGVGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAIVGA
CCHHHHHHHHHHHCCCCCCCHHHCCCEEEEECHHHCCCHHHHHHHHHHCCCCHHHHHHHH
VLAGLARTGLAQAAGAGGTPDATLRAAARLATGDLAGAADALPGVGHAVLLASYTHAFDR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHH
LLIGLAVVTVLCAGVVFAFLGGRPAATETGDDSDEHAHADVPVRSRIGAACAETRGR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]