Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 78064753

GI number: 78064753

Start: 111434

End: 112120

Strand: Direct

Name: gidB

Synonym: Bcep18194_A3276

Alternate gene names: 78064753

Gene position: 111434-112120 (Clockwise)

Preceding gene: 78064752

Following gene: 78064754

Centisome position: 3.02

GC content: 62.15

Gene sequence:

>687_bases
ATGACGGCGCGTCGCGCGCCGGCGGTTAATCGGGATGGTCTGGAACAGATGCTCGTCGAAGGCACGACGGCGCTCGACCT
CGCATTGACGGATGCACAACACAACCAGCTGCTCGACTATGTCGCGCTGCTCGGCAAGTGGAACGCGGTCTACAACCTGA
CCGCGATCCGCGACCCGAAGCAGATGCTGATCCAGCACATCCTCGATTCGCTCTCCATCGTCTCGCATCTGCGTGGCCGT
GCATCGGCCCGCGTGCTCGACGTCGGCTCGGGCGGCGGATTGCCTGGTATCGTGCTGGCGATCGTCGAGCCGGACTGGCA
AATCACGCTGAACGATATCGTGCAGAAGAAGTCTGCATTCCAGACTCAGATGCGCGCGGAGCTGAAGCTCGCGAACCTGT
CGGTGGTCACCGGGCGGGTCGAATCGCTGCAGCCGGGTGTCGAAGTGCCGGAAAAATTCGACATGATCGTATCCCGCGCT
TTCGCGGATCTGTCCGACTTCGTTAAACTTGCTCGACATCTGGTCGCGCCGGGCGGATCGATCTGGGCGATGAAGGGCGT
CCACCCGGACGACGAGATTGCGCGATTGCCGGAAGGCAGCCGCGTGAAGCAGACGATACGGCTGGCGGTACCGATGCTCG
ATGCCGAACGGCATCTGATCGAAGTGGCCGTCGACGAGGCGAATTGA

Upstream 100 bases:

>100_bases
CGCTGCTGATGGTGCACCTGAAGCGCCGCGGCCTGGGCCGCCGTAATGGCACTGCCGAAGCGGCAACGGAGCAGGGGGAC
GGCGCCGTCCCGACGCAACA

Downstream 100 bases:

>100_bases
AGGGCAGGCGCGCAGTAGCGCGCCGTTTGTTTGAAGGTAAAGGGAACACACCAACGATGGCAAAGATCTTCTGCGTTGCG
AACCAGAAGGGGGGCGTCGG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTARRAPAVNRDGLEQMLVEGTTALDLALTDAQHNQLLDYVALLGKWNAVYNLTAIRDPKQMLIQHILDSLSIVSHLRGR
ASARVLDVGSGGGLPGIVLAIVEPDWQITLNDIVQKKSAFQTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRA
FADLSDFVKLARHLVAPGGSIWAMKGVHPDDEIARLPEGSRVKQTIRLAVPMLDAERHLIEVAVDEAN

Sequences:

>Translated_228_residues
MTARRAPAVNRDGLEQMLVEGTTALDLALTDAQHNQLLDYVALLGKWNAVYNLTAIRDPKQMLIQHILDSLSIVSHLRGR
ASARVLDVGSGGGLPGIVLAIVEPDWQITLNDIVQKKSAFQTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRA
FADLSDFVKLARHLVAPGGSIWAMKGVHPDDEIARLPEGSRVKQTIRLAVPMLDAERHLIEVAVDEAN
>Mature_227_residues
TARRAPAVNRDGLEQMLVEGTTALDLALTDAQHNQLLDYVALLGKWNAVYNLTAIRDPKQMLIQHILDSLSIVSHLRGRA
SARVLDVGSGGGLPGIVLAIVEPDWQITLNDIVQKKSAFQTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRAF
ADLSDFVKLARHLVAPGGSIWAMKGVHPDDEIARLPEGSRVKQTIRLAVPMLDAERHLIEVAVDEAN

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=202, Percent_Identity=46.5346534653465, Blast_Score=181, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 24920; Mature: 24789

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARRAPAVNRDGLEQMLVEGTTALDLALTDAQHNQLLDYVALLGKWNAVYNLTAIRDPK
CCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHH
QMLIQHILDSLSIVSHLRGRASARVLDVGSGGGLPGIVLAIVEPDWQITLNDIVQKKSAF
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCEEEHHHHHHHHHHH
QTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRAFADLSDFVKLARHLVAPGGS
HHHHHHHHHHHHHHEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IWAMKGVHPDDEIARLPEGSRVKQTIRLAVPMLDAERHLIEVAVDEAN
EEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEEEEECCCC
>Mature Secondary Structure 
TARRAPAVNRDGLEQMLVEGTTALDLALTDAQHNQLLDYVALLGKWNAVYNLTAIRDPK
CCCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHH
QMLIQHILDSLSIVSHLRGRASARVLDVGSGGGLPGIVLAIVEPDWQITLNDIVQKKSAF
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCEEEHHHHHHHHHHH
QTQMRAELKLANLSVVTGRVESLQPGVEVPEKFDMIVSRAFADLSDFVKLARHLVAPGGS
HHHHHHHHHHHHHHEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IWAMKGVHPDDEIARLPEGSRVKQTIRLAVPMLDAERHLIEVAVDEAN
EEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA