| Definition | Burkholderia sp. 383 chromosome 1, complete genome. |
|---|---|
| Accession | NC_007510 |
| Length | 3,694,126 |
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The map label for this gene is braG [H]
Identifier: 78064743
GI number: 78064743
Start: 98745
End: 99500
Strand: Direct
Name: braG [H]
Synonym: Bcep18194_A3266
Alternate gene names: 78064743
Gene position: 98745-99500 (Clockwise)
Preceding gene: 78064742
Following gene: 78064744
Centisome position: 2.67
GC content: 67.06
Gene sequence:
>756_bases ATGAAGCACAGCGAACGGGAGGAACGCGAATTGAGCGGCGTCGAAAGCGGCACGCCCGCGCTGGAGATCATGGGCCTGGA GGCCTGGTACGGGGAATCCCACATATTGCACGGTGTCGACCTGACGGTGCATCGCGGCGAGGTCGTCACGCTGCTCGGCC GCAACGGCGCGGGCCGCACGACGACGCTGCGCGCGATCATGGGCCTCACGGGCCGCCGCAGCGGGTCGATCAAGGTCGCG GGCAACGAGACGATCGGCCTCGCGACGCACCGCATCGCGCATTTCGGTATCGGCTACTGCCCGGAGGAGCGCGGGATCTT CTCGAGCCTGTCGTGCGAGGAGAACCTGATGCTGCCGCCGCCGATCGGTTCGCGCGACCACGCGATGTCGATCGACGAGA TCTACACGATGTTCCCCAACCTCGCGTCGCGCCGGCAGAGCCAGGGCACGCGGCTCTCAGGCGGCGAGCAGCAGATGCTC GCGGTCGCGCGGATCCTGCGCACCGGCGCCAACCTGCTGCTGCTCGACGAGATTTCCGAGGGCCTCGCACCGGTGATCGT GCAGGCGCTGGCGCGGATGATCGTCGCGCTGAAGGCGCGCGGCTACACGATCGTGATGGTCGAACAGAATTTCCGCTTCG CGGCACCGCTCGCCGACCGGTTCTACGTGATGGAGCACGGCAGCATCGTCGAACATTTCCAGGCGGCCGAACTGGAAAGC AAGATGCCGACCCTGCACGACCTGCTCGGGGTGTGA
Upstream 100 bases:
>100_bases AGGTGCTGGCCGAAGGCTCGTATGCGGAAGTGTCGAAGAATCCGCTCGTCATCGAGGCCTACATGGGCAGCGCCGACGCG GCGCTCGCGGGGGCGCACGC
Downstream 100 bases:
>100_bases CGCCGCCCCGTCTCTCTCGCCGCTAGCCGTGGCGGCTTCGAACAACCACAACGCATCAGAACAACAGGAGACGTCAATGA AAATGAAGACCCTCGCACAC
Product: branched chain amino acid ABC transporter ATPase
Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]
Alternate protein names: NA
Number of amino acids: Translated: 251; Mature: 251
Protein sequence:
>251_residues MKHSEREERELSGVESGTPALEIMGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRTTTLRAIMGLTGRRSGSIKVA GNETIGLATHRIAHFGIGYCPEERGIFSSLSCEENLMLPPPIGSRDHAMSIDEIYTMFPNLASRRQSQGTRLSGGEQQML AVARILRTGANLLLLDEISEGLAPVIVQALARMIVALKARGYTIVMVEQNFRFAAPLADRFYVMEHGSIVEHFQAAELES KMPTLHDLLGV
Sequences:
>Translated_251_residues MKHSEREERELSGVESGTPALEIMGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRTTTLRAIMGLTGRRSGSIKVA GNETIGLATHRIAHFGIGYCPEERGIFSSLSCEENLMLPPPIGSRDHAMSIDEIYTMFPNLASRRQSQGTRLSGGEQQML AVARILRTGANLLLLDEISEGLAPVIVQALARMIVALKARGYTIVMVEQNFRFAAPLADRFYVMEHGSIVEHFQAAELES KMPTLHDLLGV >Mature_251_residues MKHSEREERELSGVESGTPALEIMGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRTTTLRAIMGLTGRRSGSIKVA GNETIGLATHRIAHFGIGYCPEERGIFSSLSCEENLMLPPPIGSRDHAMSIDEIYTMFPNLASRRQSQGTRLSGGEQQML AVARILRTGANLLLLDEISEGLAPVIVQALARMIVALKARGYTIVMVEQNFRFAAPLADRFYVMEHGSIVEHFQAAELES KMPTLHDLLGV
Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]
COG id: COG0410
COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component
Gene ontology:
Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ABC transporter domain [H]
Homologues:
Organism=Homo sapiens, GI47078218, Length=200, Percent_Identity=31, Blast_Score=83, Evalue=3e-16, Organism=Homo sapiens, GI45446740, Length=200, Percent_Identity=31, Blast_Score=82, Evalue=3e-16, Organism=Homo sapiens, GI21536376, Length=196, Percent_Identity=27.0408163265306, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI31657092, Length=235, Percent_Identity=25.531914893617, Blast_Score=80, Evalue=2e-15, Organism=Homo sapiens, GI27881501, Length=220, Percent_Identity=28.1818181818182, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI30795238, Length=220, Percent_Identity=28.1818181818182, Blast_Score=79, Evalue=3e-15, Organism=Homo sapiens, GI105990541, Length=193, Percent_Identity=26.4248704663212, Blast_Score=75, Evalue=8e-14, Organism=Homo sapiens, GI153792144, Length=225, Percent_Identity=25.3333333333333, Blast_Score=72, Evalue=5e-13, Organism=Homo sapiens, GI116734710, Length=193, Percent_Identity=28.4974093264249, Blast_Score=72, Evalue=6e-13, Organism=Homo sapiens, GI27436953, Length=195, Percent_Identity=27.6923076923077, Blast_Score=72, Evalue=6e-13, Organism=Homo sapiens, GI150417984, Length=217, Percent_Identity=26.2672811059908, Blast_Score=71, Evalue=1e-12, Organism=Homo sapiens, GI27262624, Length=219, Percent_Identity=26.4840182648402, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI27262626, Length=219, Percent_Identity=26.4840182648402, Blast_Score=67, Evalue=1e-11, Organism=Homo sapiens, GI9961252, Length=215, Percent_Identity=28.3720930232558, Blast_Score=65, Evalue=4e-11, Organism=Homo sapiens, GI4505771, Length=215, Percent_Identity=28.3720930232558, Blast_Score=65, Evalue=4e-11, Organism=Homo sapiens, GI9955963, Length=206, Percent_Identity=28.6407766990291, Blast_Score=65, Evalue=5e-11, Organism=Homo sapiens, GI9961250, Length=215, Percent_Identity=28.3720930232558, Blast_Score=65, Evalue=5e-11, Organism=Homo sapiens, GI27477115, Length=221, Percent_Identity=28.5067873303167, Blast_Score=65, Evalue=8e-11, Organism=Escherichia coli, GI87082268, Length=206, Percent_Identity=42.2330097087379, Blast_Score=151, Evalue=4e-38, Organism=Escherichia coli, GI1789593, Length=222, Percent_Identity=31.981981981982, Blast_Score=109, Evalue=2e-25, Organism=Escherichia coli, GI1787792, Length=230, Percent_Identity=30, Blast_Score=99, Evalue=2e-22, Organism=Escherichia coli, GI1789672, Length=230, Percent_Identity=31.304347826087, Blast_Score=96, Evalue=3e-21, Organism=Escherichia coli, GI1788225, Length=230, Percent_Identity=32.1739130434783, Blast_Score=95, Evalue=5e-21, Organism=Escherichia coli, GI1787029, Length=215, Percent_Identity=28.3720930232558, Blast_Score=91, Evalue=7e-20, Organism=Escherichia coli, GI1787089, Length=225, Percent_Identity=29.7777777777778, Blast_Score=90, Evalue=1e-19, Organism=Escherichia coli, GI1788450, Length=216, Percent_Identity=29.1666666666667, Blast_Score=87, Evalue=8e-19, Organism=Escherichia coli, GI1789864, Length=228, Percent_Identity=26.7543859649123, Blast_Score=86, Evalue=3e-18, Organism=Escherichia coli, GI1790190, Length=224, Percent_Identity=25.8928571428571, Blast_Score=85, Evalue=4e-18, Organism=Escherichia coli, GI1788897, Length=227, Percent_Identity=29.5154185022026, Blast_Score=84, Evalue=9e-18, Organism=Escherichia coli, GI48995001, Length=221, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=1e-17, Organism=Escherichia coli, GI1788761, Length=221, Percent_Identity=30.316742081448, Blast_Score=82, Evalue=5e-17, Organism=Escherichia coli, GI1790525, Length=224, Percent_Identity=27.6785714285714, Blast_Score=81, Evalue=7e-17, Organism=Escherichia coli, GI1787370, Length=225, Percent_Identity=30.6666666666667, Blast_Score=78, Evalue=5e-16, Organism=Escherichia coli, GI1786319, Length=231, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=8e-16, Organism=Escherichia coli, GI1787105, Length=231, Percent_Identity=26.8398268398268, Blast_Score=77, Evalue=1e-15, Organism=Escherichia coli, GI87081791, Length=220, Percent_Identity=29.0909090909091, Blast_Score=76, Evalue=2e-15, Organism=Escherichia coli, GI1786253, Length=224, Percent_Identity=29.4642857142857, Blast_Score=75, Evalue=4e-15, Organism=Escherichia coli, GI1786872, Length=218, Percent_Identity=28.8990825688073, Blast_Score=75, Evalue=5e-15, Organism=Escherichia coli, GI1786398, Length=211, Percent_Identity=26.0663507109005, Blast_Score=72, Evalue=5e-14, Organism=Escherichia coli, GI1786703, Length=208, Percent_Identity=28.8461538461538, Blast_Score=71, Evalue=5e-14, Organism=Escherichia coli, GI87081782, Length=169, Percent_Identity=31.9526627218935, Blast_Score=70, Evalue=1e-13, Organism=Escherichia coli, GI1787112, Length=218, Percent_Identity=32.1100917431193, Blast_Score=70, Evalue=1e-13, Organism=Escherichia coli, GI1789991, Length=230, Percent_Identity=27.3913043478261, Blast_Score=70, Evalue=2e-13, Organism=Escherichia coli, GI1787712, Length=220, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=6e-13, Organism=Escherichia coli, GI1788644, Length=228, Percent_Identity=28.9473684210526, Blast_Score=67, Evalue=9e-13, Organism=Escherichia coli, GI1786698, Length=173, Percent_Identity=26.0115606936416, Blast_Score=67, Evalue=2e-12, Organism=Escherichia coli, GI1788472, Length=228, Percent_Identity=26.7543859649123, Blast_Score=66, Evalue=2e-12, Organism=Escherichia coli, GI87081709, Length=218, Percent_Identity=27.5229357798165, Blast_Score=65, Evalue=5e-12, Organism=Escherichia coli, GI1790467, Length=216, Percent_Identity=26.8518518518519, Blast_Score=64, Evalue=1e-11, Organism=Escherichia coli, GI1786563, Length=210, Percent_Identity=24.7619047619048, Blast_Score=64, Evalue=1e-11, Organism=Escherichia coli, GI1790544, Length=234, Percent_Identity=25.2136752136752, Blast_Score=62, Evalue=3e-11, Organism=Escherichia coli, GI1788165, Length=212, Percent_Identity=25.9433962264151, Blast_Score=62, Evalue=3e-11, Organism=Escherichia coli, GI1788210, Length=225, Percent_Identity=24, Blast_Score=61, Evalue=7e-11, Organism=Caenorhabditis elegans, GI17510237, Length=225, Percent_Identity=29.7777777777778, Blast_Score=86, Evalue=1e-17, Organism=Caenorhabditis elegans, GI115533608, Length=212, Percent_Identity=29.2452830188679, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI71996809, Length=225, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=3e-14, Organism=Caenorhabditis elegans, GI193211017, Length=225, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI193211015, Length=225, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI71983523, Length=211, Percent_Identity=27.9620853080569, Blast_Score=71, Evalue=4e-13, Organism=Caenorhabditis elegans, GI25146777, Length=215, Percent_Identity=26.9767441860465, Blast_Score=71, Evalue=4e-13, Organism=Caenorhabditis elegans, GI71983516, Length=211, Percent_Identity=27.9620853080569, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI71983510, Length=211, Percent_Identity=27.9620853080569, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI212646699, Length=208, Percent_Identity=27.4038461538462, Blast_Score=69, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17565586, Length=242, Percent_Identity=26.0330578512397, Blast_Score=68, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17565938, Length=244, Percent_Identity=25.4098360655738, Blast_Score=66, Evalue=1e-11, Organism=Caenorhabditis elegans, GI115535101, Length=207, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=3e-11, Organism=Drosophila melanogaster, GI24648314, Length=213, Percent_Identity=27.2300469483568, Blast_Score=90, Evalue=1e-18, Organism=Drosophila melanogaster, GI24666092, Length=212, Percent_Identity=28.7735849056604, Blast_Score=87, Evalue=1e-17, Organism=Drosophila melanogaster, GI221512771, Length=214, Percent_Identity=27.5700934579439, Blast_Score=80, Evalue=1e-15, Organism=Drosophila melanogaster, GI116007328, Length=190, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=4e-14, Organism=Drosophila melanogaster, GI28574150, Length=190, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=4e-14, Organism=Drosophila melanogaster, GI24643674, Length=230, Percent_Identity=27.3913043478261, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI24650853, Length=221, Percent_Identity=25.7918552036199, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI24650855, Length=221, Percent_Identity=25.7918552036199, Blast_Score=67, Evalue=1e-11, Organism=Drosophila melanogaster, GI85725262, Length=187, Percent_Identity=28.8770053475936, Blast_Score=66, Evalue=2e-11, Organism=Drosophila melanogaster, GI21357907, Length=216, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=4e-11, Organism=Drosophila melanogaster, GI281362751, Length=228, Percent_Identity=25.8771929824561, Blast_Score=65, Evalue=5e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003439 - InterPro: IPR017871 - InterPro: IPR003593 [H]
Pfam domain/function: PF00005 ABC_tran [H]
EC number: NA
Molecular weight: Translated: 27459; Mature: 27459
Theoretical pI: Translated: 6.51; Mature: 6.51
Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 4.4 %Met (Translated Protein) 5.2 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 4.4 %Met (Mature Protein) 5.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKHSEREERELSGVESGTPALEIMGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRT CCCCHHHHHHHCCCCCCCCEEEEEEEHHHCCCCEEEECEEEEEECCCEEEEEECCCCCCH TTLRAIMGLTGRRSGSIKVAGNETIGLATHRIAHFGIGYCPEERGIFSSLSCEENLMLPP HHHHHHHHCCCCCCCCEEECCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC PIGSRDHAMSIDEIYTMFPNLASRRQSQGTRLSGGEQQMLAVARILRTGANLLLLDEISE CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC GLAPVIVQALARMIVALKARGYTIVMVEQNFRFAAPLADRFYVMEHGSIVEHFQAAELES CHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCEEEEEECCCHHHHHHHHHHHH KMPTLHDLLGV CCCHHHHHHCC >Mature Secondary Structure MKHSEREERELSGVESGTPALEIMGLEAWYGESHILHGVDLTVHRGEVVTLLGRNGAGRT CCCCHHHHHHHCCCCCCCCEEEEEEEHHHCCCCEEEECEEEEEECCCEEEEEECCCCCCH TTLRAIMGLTGRRSGSIKVAGNETIGLATHRIAHFGIGYCPEERGIFSSLSCEENLMLPP HHHHHHHHCCCCCCCCEEECCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC PIGSRDHAMSIDEIYTMFPNLASRRQSQGTRLSGGEQQMLAVARILRTGANLLLLDEISE CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHC GLAPVIVQALARMIVALKARGYTIVMVEQNFRFAAPLADRFYVMEHGSIVEHFQAAELES CHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCCEEEEEECCCHHHHHHHHHHHH KMPTLHDLLGV CCCHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]
Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 7.0
TargetDB status: NA
Availability: NA
References: 2120183; 10984043 [H]