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The map label for this gene is mnmC

Identifier: 78064725

GI number: 78064725

Start: 80191

End: 82137

Strand: Direct

Name: mnmC

Synonym: Bcep18194_A3248

Alternate gene names: 78064725

Gene position: 80191-82137 (Clockwise)

Preceding gene: 78064723

Following gene: 78064726

Centisome position: 2.17

GC content: 69.95

Gene sequence:

>1947_bases
ATGACCGACCGACTTGTTCCCGCCTCGCTCGCGCTTCGCGACGACGGCACGCTCGTCTCGCCCGAGTTCGGCGACCTCCA
TCTCGGCGCATCAGGTGCGCTTGCGCATCGCGTGTTCGTCGCCGGCAACGGCCTGCCGACGCGCTGGCAGGACCGCCGCA
CGTTCACGATCGTGGCGACCGGATTCGGCGCGGGCGGCAGTTTCCTTGCGGCCTGGGCCGCGTGGCGTGACGATCCCGCG
CGCTGCGAGCGGTTGCATTTTGTCGCAGTCGAACCGCATCCGTTCTCGCGCGACGACCTGCGCCGCGCAGCTACTCATAT
CGTTGCGGACACAACCATATCGGCGGATGTCGAGGCACTTGCCGACGCGTGGCCGATGCTTGTACCGGGTCTGCACCGGC
TCGAATTCGACGAAGGGCGCGTGGTGCTCACGCTCGCGTTCGGCGAGTCGATCGACATGCTGGGGAAGATCGTCGCGCGC
GCCGACGCGTTCTGTCTCGACGGCCTCGCGTCGTCGAGCGACGCGGATCTCCAATCGACCGACGTGGTCCGCGCGCTCTC
GAAAATCGCCGGCGAACACGCAACGTTTGCAGTGCATGCGAGTTCGGACGCGTTGAAACACGCACTCGGCAAATCGGGTT
TCACGTATCGCGAGGTCGACGATCTGCTCGTCGGCGAATATGCGCCGCGCTGGCGCGCGCGCCGGCACGAGCCGCCGCTC
GCACTGCCGGTTGCGACGCGCCGCGCGATCGTGATCGGTGCGGGCCTGGCGGGCTGCGCGGTCGTCGAGCGGCTGGCCGC
GCGCGGCTGGGAAGTCACGCTGATCGAGCGGCACGACCGGATCGCGAGCGACGCATCGGGCAATCCGGCCGGTGTATTCC
ATCCGTTGATGACACGCGACGACAACGTCGCGAGCCGGCTCACGCGCGGTGGCTTCCTGCATGCGATCGCACGCTGGCGC
GCACTCGAAAACGCAGGCCATGCATTCGCGCGCAGTACGAACGGGATGATCCATCTCGCCGAATCGGCCGACGACTTCGA
ACGGATGCGCGATGCATTCGATGCGTTCGGCCCGCCGTCCGACTATGTCTCGCTGCTCGACATCGAGGCCGCGCGCGCGC
ATCTGAACCTGTCGGTCGCGCAGGGCGGCCTGCTGTTTCCGCATGGCGGCGCTGTCTGGCCAGCCGGCCTTTGCGCGGCT
CAATACGCGGCGGCCGGCGAACGCGTGCGCTTGCTCGCGTCCACCCGCGTTGCAAAACTCGAACGGCGGGGCGACGCATG
GCATGCACTCGACGATGCGGGCGGCACGCTGGCCGAAGCGCCCGTCGTCGTGCTCGCGAATGCCGGCGACGCCGCGCGCC
TCGCCGGGCTGCAGCATGTCGCGCTGCAACCGGTGCGTGGCCAGCTCACGTTGTTGCCGCCCGGCAGTACGGCACCGCTG
CCGTGCCCGACGATCGGCGACGGCTACGCGGTGCCGCTCGACGACGGCACGCTGCTGATCGGCGCAACGTTCGAACCCGA
CGACGTCGATCCCGCGATGCGCGCGGCCGGCCACCTCGAAAACCTCGAACGCGTGCGGCATCTGCTGCCGGGCCTGATCG
GCGACCTGCCGGACCCCGACACACTGCGCGGCCGCGTCGCATTCCGTTGGGTCGTCGGCGACCGCCTGCCGCTGCTCGGC
CCGCTCGCGGATGAAGCCGGCGCCATCGCCAATGCACGCGCGCTGAGCGGTGCGCAGGCGCGCGACCTGCCGCGCCTGCC
CGGGCTCTACGGCGCATTCGGCTTCGGCTCGCGCGGCCTCGTATGGGCTGCACTCGGCGCCGAACTGATCGCATCGCAAC
TCGAAGGCGAGCCCTGGCCGCTCGAACGCGAACTCGCCGATGCCGTCGACCCCGCACGCTTCCTGATCCGCGCGTTGCGC
GCCCGCCGCGTCGGCCGGGCCGGCTGA

Upstream 100 bases:

>100_bases
CGGTCCGCCCGTGCGCCGCGCCGTTGGCTTTTTCCAACGACCGGTCGGACGGCCCCGCGCAATCCCTTGCCAGGCTTGGC
TGCCACGTTTTTTGTTTCGC

Downstream 100 bases:

>100_bases
TCGCTCACCCGAACCAATCACAGTTTCCCGACTTTTTCGCGCAAGGTTATCCACGCCGCACTGTGGATAACCGCGCGGAA
TCCGTGCGCACTTCGTGTGC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MTDRLVPASLALRDDGTLVSPEFGDLHLGASGALAHRVFVAGNGLPTRWQDRRTFTIVATGFGAGGSFLAAWAAWRDDPA
RCERLHFVAVEPHPFSRDDLRRAATHIVADTTISADVEALADAWPMLVPGLHRLEFDEGRVVLTLAFGESIDMLGKIVAR
ADAFCLDGLASSSDADLQSTDVVRALSKIAGEHATFAVHASSDALKHALGKSGFTYREVDDLLVGEYAPRWRARRHEPPL
ALPVATRRAIVIGAGLAGCAVVERLAARGWEVTLIERHDRIASDASGNPAGVFHPLMTRDDNVASRLTRGGFLHAIARWR
ALENAGHAFARSTNGMIHLAESADDFERMRDAFDAFGPPSDYVSLLDIEAARAHLNLSVAQGGLLFPHGGAVWPAGLCAA
QYAAAGERVRLLASTRVAKLERRGDAWHALDDAGGTLAEAPVVVLANAGDAARLAGLQHVALQPVRGQLTLLPPGSTAPL
PCPTIGDGYAVPLDDGTLLIGATFEPDDVDPAMRAAGHLENLERVRHLLPGLIGDLPDPDTLRGRVAFRWVVGDRLPLLG
PLADEAGAIANARALSGAQARDLPRLPGLYGAFGFGSRGLVWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALR
ARRVGRAG

Sequences:

>Translated_648_residues
MTDRLVPASLALRDDGTLVSPEFGDLHLGASGALAHRVFVAGNGLPTRWQDRRTFTIVATGFGAGGSFLAAWAAWRDDPA
RCERLHFVAVEPHPFSRDDLRRAATHIVADTTISADVEALADAWPMLVPGLHRLEFDEGRVVLTLAFGESIDMLGKIVAR
ADAFCLDGLASSSDADLQSTDVVRALSKIAGEHATFAVHASSDALKHALGKSGFTYREVDDLLVGEYAPRWRARRHEPPL
ALPVATRRAIVIGAGLAGCAVVERLAARGWEVTLIERHDRIASDASGNPAGVFHPLMTRDDNVASRLTRGGFLHAIARWR
ALENAGHAFARSTNGMIHLAESADDFERMRDAFDAFGPPSDYVSLLDIEAARAHLNLSVAQGGLLFPHGGAVWPAGLCAA
QYAAAGERVRLLASTRVAKLERRGDAWHALDDAGGTLAEAPVVVLANAGDAARLAGLQHVALQPVRGQLTLLPPGSTAPL
PCPTIGDGYAVPLDDGTLLIGATFEPDDVDPAMRAAGHLENLERVRHLLPGLIGDLPDPDTLRGRVAFRWVVGDRLPLLG
PLADEAGAIANARALSGAQARDLPRLPGLYGAFGFGSRGLVWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALR
ARRVGRAG
>Mature_647_residues
TDRLVPASLALRDDGTLVSPEFGDLHLGASGALAHRVFVAGNGLPTRWQDRRTFTIVATGFGAGGSFLAAWAAWRDDPAR
CERLHFVAVEPHPFSRDDLRRAATHIVADTTISADVEALADAWPMLVPGLHRLEFDEGRVVLTLAFGESIDMLGKIVARA
DAFCLDGLASSSDADLQSTDVVRALSKIAGEHATFAVHASSDALKHALGKSGFTYREVDDLLVGEYAPRWRARRHEPPLA
LPVATRRAIVIGAGLAGCAVVERLAARGWEVTLIERHDRIASDASGNPAGVFHPLMTRDDNVASRLTRGGFLHAIARWRA
LENAGHAFARSTNGMIHLAESADDFERMRDAFDAFGPPSDYVSLLDIEAARAHLNLSVAQGGLLFPHGGAVWPAGLCAAQ
YAAAGERVRLLASTRVAKLERRGDAWHALDDAGGTLAEAPVVVLANAGDAARLAGLQHVALQPVRGQLTLLPPGSTAPLP
CPTIGDGYAVPLDDGTLLIGATFEPDDVDPAMRAAGHLENLERVRHLLPGLIGDLPDPDTLRGRVAFRWVVGDRLPLLGP
LADEAGAIANARALSGAQARDLPRLPGLYGAFGFGSRGLVWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALRA
RRVGRAG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=680, Percent_Identity=27.0588235294118, Blast_Score=214, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_BURS3 (Q39L16)

Other databases:

- EMBL:   CP000151
- RefSeq:   YP_367494.1
- ProteinModelPortal:   Q39L16
- SMR:   Q39L16
- STRING:   Q39L16
- GeneID:   3748394
- GenomeReviews:   CP000151_GR
- KEGG:   bur:Bcep18194_A3248
- NMPDR:   fig|269483.3.peg.5170
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- BioCyc:   BSP36773:BCEP18194_A3248-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR016040
- InterPro:   IPR023032
- InterPro:   IPR017610
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 68931; Mature: 68800

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRLVPASLALRDDGTLVSPEFGDLHLGASGALAHRVFVAGNGLPTRWQDRRTFTIVAT
CCCCCCCCEEEEECCCCEECCCCCCEEECCCCHHEEEEEEECCCCCCCCCCCEEEEEEEE
GFGAGGSFLAAWAAWRDDPARCERLHFVAVEPHPFSRDDLRRAATHIVADTTISADVEAL
CCCCCCHHHHHHHHHCCCHHHHCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
ADAWPMLVPGLHRLEFDEGRVVLTLAFGESIDMLGKIVARADAFCLDGLASSSDADLQST
HHHHHHHCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
DVVRALSKIAGEHATFAVHASSDALKHALGKSGFTYREVDDLLVGEYAPRWRARRHEPPL
HHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCE
ALPVATRRAIVIGAGLAGCAVVERLAARGWEVTLIERHDRIASDASGNPAGVFHPLMTRD
EEECCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCEECHHHCCC
DNVASRLTRGGFLHAIARWRALENAGHAFARSTNGMIHLAESADDFERMRDAFDAFGPPS
CHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCH
DYVSLLDIEAARAHLNLSVAQGGLLFPHGGAVWPAGLCAAQYAAAGERVRLLASTRVAKL
HHHHHHHHHHHHHEEEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHH
ERRGDAWHALDDAGGTLAEAPVVVLANAGDAARLAGLQHVALQPVRGQLTLLPPGSTAPL
HHCCCCCEEHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
PCPTIGDGYAVPLDDGTLLIGATFEPDDVDPAMRAAGHLENLERVRHLLPGLIGDLPDPD
CCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TLRGRVAFRWVVGDRLPLLGPLADEAGAIANARALSGAQARDLPRLPGLYGAFGFGSRGL
CCCCEEEEEEEECCCCCCCCCCCHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
VWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVGRAG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDRLVPASLALRDDGTLVSPEFGDLHLGASGALAHRVFVAGNGLPTRWQDRRTFTIVAT
CCCCCCCEEEEECCCCEECCCCCCEEECCCCHHEEEEEEECCCCCCCCCCCEEEEEEEE
GFGAGGSFLAAWAAWRDDPARCERLHFVAVEPHPFSRDDLRRAATHIVADTTISADVEAL
CCCCCCHHHHHHHHHCCCHHHHCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
ADAWPMLVPGLHRLEFDEGRVVLTLAFGESIDMLGKIVARADAFCLDGLASSSDADLQST
HHHHHHHCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
DVVRALSKIAGEHATFAVHASSDALKHALGKSGFTYREVDDLLVGEYAPRWRARRHEPPL
HHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCE
ALPVATRRAIVIGAGLAGCAVVERLAARGWEVTLIERHDRIASDASGNPAGVFHPLMTRD
EEECCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCEECHHHCCC
DNVASRLTRGGFLHAIARWRALENAGHAFARSTNGMIHLAESADDFERMRDAFDAFGPPS
CHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCH
DYVSLLDIEAARAHLNLSVAQGGLLFPHGGAVWPAGLCAAQYAAAGERVRLLASTRVAKL
HHHHHHHHHHHHHEEEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHH
ERRGDAWHALDDAGGTLAEAPVVVLANAGDAARLAGLQHVALQPVRGQLTLLPPGSTAPL
HHCCCCCEEHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
PCPTIGDGYAVPLDDGTLLIGATFEPDDVDPAMRAAGHLENLERVRHLLPGLIGDLPDPD
CCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TLRGRVAFRWVVGDRLPLLGPLADEAGAIANARALSGAQARDLPRLPGLYGAFGFGSRGL
CCCCEEEEEEEECCCCCCCCCCCHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
VWAALGAELIASQLEGEPWPLERELADAVDPARFLIRALRARRVGRAG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA