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The map label for this gene is ywbI [H]

Identifier: 78064705

GI number: 78064705

Start: 59917

End: 60843

Strand: Direct

Name: ywbI [H]

Synonym: Bcep18194_A3228

Alternate gene names: 78064705

Gene position: 59917-60843 (Clockwise)

Preceding gene: 78064688

Following gene: 78064708

Centisome position: 1.62

GC content: 69.04

Gene sequence:

>927_bases
ATGGAATTGCGCGCGCTGCGGTACTTCGTCGAGGTGGTTCGCCAGCAGAGTTTCACGGCGGCCGCCGACAAGCTGTTCGT
GACCCAGCCGACCATCAGCAAGATGGTGAAGGCGCTCGAGGACGAGATCGGTTCGCCGCTGCTGCTACGCGACGGCCGGC
AGATGGTGCTGACCGATGCAGGGCGGATCGTGTTCCAGCGTGGCCAGGACGTGCTCGCCGCGCAGGCGCAGCTGCAGTCG
GAGCTGAACGATCTCGGCACGCTCGGGCGCGGCGAGCTGACGATCGGGATCCCGCCGCTCGGCGGCTCGCTGTTCACGCC
GATCATCGCCGCGTACAAGCAGCGCTATCCGAACATCGAGCTGAAGCTGTTCGAGCAGGGTGCGCGGATGATCGAGGCCG
CGCTGACGTCGGGAGAGCTGGAGCTGGGCGGGCTGCTGGAGCCGGTCGATCCCGGCACCTTCGAGCGGCTGCCGATGGTG
CGCGCGCCGCTGTGGCTCGTCGCCCCGCGCGAGTCGCGCTGGGAGGATCATGCGGCCGTGCCGCTCGCGGATCTCGCGCG
CGAGTCGTTTGTGTTCTACGCGGAAAGCCTCGCGCTGCACGACGCGGTGCTCGACGCGTGCCGGCAAGTTGGCTTCACCC
CGTCGATCGTGAGCCGCAGCGGCCACTGGGATTTCATGGCCGCGCTCGTGCATGCGGGGGTCGGTATCGCGCTGCTGCCC
GAGCCGTATTGCCGTCGGCTCGACGCAGGCCAGTTCACGTGCCGGCCGATCGTCGAGCCGGAGATCACGTGGGCGATCGC
GCTCGGCTGGCTGAAGAAGGGGTATCTGTCGCACGCGGCGCGCGCGTGGCTTGAAGTGGCGCGGGCAACGCTGCCGATCG
CGCCGGGTGACGATCTGGCGTTCGGCGGGTTGCGGGCGAGGGAGTGA

Upstream 100 bases:

>100_bases
ATAGGGTTTCCCCTAACCATAAATAAAATGACTTGTCTCTATCCTTGTCATTCCAATTCGGAATTCGTTTTGATCGCCGC
ACCAATTTGGGGAGTCATCG

Downstream 100 bases:

>100_bases
CGCCCGCCGAAGCGGGCGGTAAATCGAACGAGGCGGGCGCAATGGCCGCCCGCCGCGCCTTCAATGCCCCATCGCCGGCC
CCGCACCCTTTCTCGGCTTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MELRALRYFVEVVRQQSFTAAADKLFVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDAGRIVFQRGQDVLAAQAQLQS
ELNDLGTLGRGELTIGIPPLGGSLFTPIIAAYKQRYPNIELKLFEQGARMIEAALTSGELELGGLLEPVDPGTFERLPMV
RAPLWLVAPRESRWEDHAAVPLADLARESFVFYAESLALHDAVLDACRQVGFTPSIVSRSGHWDFMAALVHAGVGIALLP
EPYCRRLDAGQFTCRPIVEPEITWAIALGWLKKGYLSHAARAWLEVARATLPIAPGDDLAFGGLRARE

Sequences:

>Translated_308_residues
MELRALRYFVEVVRQQSFTAAADKLFVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDAGRIVFQRGQDVLAAQAQLQS
ELNDLGTLGRGELTIGIPPLGGSLFTPIIAAYKQRYPNIELKLFEQGARMIEAALTSGELELGGLLEPVDPGTFERLPMV
RAPLWLVAPRESRWEDHAAVPLADLARESFVFYAESLALHDAVLDACRQVGFTPSIVSRSGHWDFMAALVHAGVGIALLP
EPYCRRLDAGQFTCRPIVEPEITWAIALGWLKKGYLSHAARAWLEVARATLPIAPGDDLAFGGLRARE
>Mature_308_residues
MELRALRYFVEVVRQQSFTAAADKLFVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDAGRIVFQRGQDVLAAQAQLQS
ELNDLGTLGRGELTIGIPPLGGSLFTPIIAAYKQRYPNIELKLFEQGARMIEAALTSGELELGGLLEPVDPGTFERLPMV
RAPLWLVAPRESRWEDHAAVPLADLARESFVFYAESLALHDAVLDACRQVGFTPSIVSRSGHWDFMAALVHAGVGIALLP
EPYCRRLDAGQFTCRPIVEPEITWAIALGWLKKGYLSHAARAWLEVARATLPIAPGDDLAFGGLRARE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=287, Percent_Identity=30.6620209059233, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI157672245, Length=296, Percent_Identity=28.7162162162162, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1790399, Length=296, Percent_Identity=30.4054054054054, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788887, Length=297, Percent_Identity=29.96632996633, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI2367136, Length=248, Percent_Identity=27.4193548387097, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=25.7028112449799, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788748, Length=293, Percent_Identity=28.6689419795222, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI145693105, Length=284, Percent_Identity=23.943661971831, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789506, Length=306, Percent_Identity=21.8954248366013, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787530, Length=245, Percent_Identity=25.7142857142857, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI145693193, Length=123, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=142, Percent_Identity=33.0985915492958, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=26.6393442622951, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=23.3606557377049, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=122, Percent_Identity=31.1475409836066, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=148, Percent_Identity=30.4054054054054, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=246, Percent_Identity=23.9837398373984, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33840; Mature: 33840

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRALRYFVEVVRQQSFTAAADKLFVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDA
CCHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHCCCEEEECCCEEEEECC
GRIVFQRGQDVLAAQAQLQSELNDLGTLGRGELTIGIPPLGGSLFTPIIAAYKQRYPNIE
CHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCE
LKLFEQGARMIEAALTSGELELGGLLEPVDPGTFERLPMVRAPLWLVAPRESRWEDHAAV
EEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHCCEEEECCCCCCCCCCCCC
PLADLARESFVFYAESLALHDAVLDACRQVGFTPSIVSRSGHWDFMAALVHAGVGIALLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCEEECC
EPYCRRLDAGQFTCRPIVEPEITWAIALGWLKKGYLSHAARAWLEVARATLPIAPGDDLA
CHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FGGLRARE
CCCCCCCC
>Mature Secondary Structure
MELRALRYFVEVVRQQSFTAAADKLFVTQPTISKMVKALEDEIGSPLLLRDGRQMVLTDA
CCHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHCCCEEEECCCEEEEECC
GRIVFQRGQDVLAAQAQLQSELNDLGTLGRGELTIGIPPLGGSLFTPIIAAYKQRYPNIE
CHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCE
LKLFEQGARMIEAALTSGELELGGLLEPVDPGTFERLPMVRAPLWLVAPRESRWEDHAAV
EEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHCCEEEECCCCCCCCCCCCC
PLADLARESFVFYAESLALHDAVLDACRQVGFTPSIVSRSGHWDFMAALVHAGVGIALLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCEEECC
EPYCRRLDAGQFTCRPIVEPEITWAIALGWLKKGYLSHAARAWLEVARATLPIAPGDDLA
CHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FGGLRARE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]