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The map label for this gene is fliN [H]

Identifier: 78064700

GI number: 78064700

Start: 56218

End: 56709

Strand: Reverse

Name: fliN [H]

Synonym: Bcep18194_A3223

Alternate gene names: 78064700

Gene position: 56709-56218 (Counterclockwise)

Preceding gene: 78064701

Following gene: 78064699

Centisome position: 1.54

GC content: 68.29

Gene sequence:

>492_bases
ATGAGTGAGCTGAACCAGACGCCCGAGGACGACGCACAAGCGATGGCCGACGCGGCCCTCGCGGCTTCGGCCGCCGCCGC
GCAGGCGGCACCGGCCGCGGCGGAAGAAGATCCGGGCATGGACGACTGGGCGGCCGCGCTCGCCGAGCAGAACCAGCAGC
CGGTGCAGGCGGGCGCGACGGGCGCCGGCGTGTTCCAGCCGCTGTCGAAGGCCGCGGCAAGCTCGACGCACAACGACATC
GAGATGATCCTCGACATCCCGGTCAAGATGACCGTGGAGCTCGGCCGCACGAAGATCGCGATCCGCAACCTGCTGCAGCT
CGCGCAGGGTTCGGTCGTCGAGCTGGACGGCATGGCCGGCGAGCCGATGGACGTGCTCGTGAACGGCTGCCTGATCGCGC
AGGGCGAAGTCGTGGTCGTCAACGACAAGTTCGGTATCCGTCTGACCGACATCATCACGCCGGCCGAACGCATCCGGAAG
CTGAATCGATGA

Upstream 100 bases:

>100_bases
GTGCCGGTGATGGAATGCGGCTACGGAATTTTCAATGGTCAATATGCGTTGCGCGTGCAGAAGATGATCAGCGCGGGCGA
TTCGATGAAGGAAGGTGGAT

Downstream 100 bases:

>100_bases
ATGTTGTGAAGCCCGGCCGCCGCGTGCGCGTCGCAAGCGTGGCGGCTGCTGCTGCCGCCATGGCGGCGTCGCTTGCCTGC
ACGCCGGCCGGCGCCGCCGA

Product: Type III secretion system outer membrane O protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MSELNQTPEDDAQAMADAALAASAAAAQAAPAAAEEDPGMDDWAAALAEQNQQPVQAGATGAGVFQPLSKAAASSTHNDI
EMILDIPVKMTVELGRTKIAIRNLLQLAQGSVVELDGMAGEPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRK
LNR

Sequences:

>Translated_163_residues
MSELNQTPEDDAQAMADAALAASAAAAQAAPAAAEEDPGMDDWAAALAEQNQQPVQAGATGAGVFQPLSKAAASSTHNDI
EMILDIPVKMTVELGRTKIAIRNLLQLAQGSVVELDGMAGEPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRK
LNR
>Mature_162_residues
SELNQTPEDDAQAMADAALAASAAAAQAAPAAAEEDPGMDDWAAALAEQNQQPVQAGATGAGVFQPLSKAAASSTHNDIE
MILDIPVKMTVELGRTKIAIRNLLQLAQGSVVELDGMAGEPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRKL
NR

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=132, Percent_Identity=57.5757575757576, Blast_Score=154, Evalue=2e-39,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 16988; Mature: 16857

Theoretical pI: Translated: 4.05; Mature: 4.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELNQTPEDDAQAMADAALAASAAAAQAAPAAAEEDPGMDDWAAALAEQNQQPVQAGAT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
GAGVFQPLSKAAASSTHNDIEMILDIPVKMTVELGRTKIAIRNLLQLAQGSVVELDGMAG
CCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHCHHHHHHHHHHHHHCCCEEEECCCCC
EPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRKLNR
CCHHHHHHCEEEECCCEEEEECCCCEEEHHHCCCHHHHHHCCC
>Mature Secondary Structure 
SELNQTPEDDAQAMADAALAASAAAAQAAPAAAEEDPGMDDWAAALAEQNQQPVQAGAT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
GAGVFQPLSKAAASSTHNDIEMILDIPVKMTVELGRTKIAIRNLLQLAQGSVVELDGMAG
CCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHCHHHHHHHHHHHHHCCCEEEECCCCC
EPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRKLNR
CCHHHHHHCEEEECCCEEEEECCCCEEEHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2651416; 9097040; 9278503 [H]