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The map label for this gene is rsmE [H]

Identifier: 229260091

GI number: 229260091

Start: 607765

End: 608532

Strand: Reverse

Name: rsmE [H]

Synonym: Bcep18194_A3719

Alternate gene names: 229260091

Gene position: 608532-607765 (Counterclockwise)

Preceding gene: 78065196

Following gene: 78065188

Centisome position: 16.47

GC content: 73.7

Gene sequence:

>768_bases
ATGAACGAAGCCACCACCACCGCGGCCGTGCCGCGCTTTTTCGTCGACGCGGCACTGCGCGCCGACGCCACGCTCGCGTT
GCCGGCCGATGTCGCGCGCCACGCGCAAGTGCTGCGCCTGCAGCCCGGCGACGTGCTCGCGCTGTTCGACGGCACCGGCG
GCCAGTACCGCGCGCGGCTCGTCGAGATCGACAAGCGCAGCGCGCTCGCGCAGATCGACACGTTCGAACCGGCCGAAGCC
GAGCCGCCCTATCGCGTGACGCTCGCGCAAGGCATCGCCGGCGGCGACAAGATGGACTGGGTGATCGAGAAGGCCGTCGA
GCTCGGCGTGGCGGCGGTCGTACCGCTGTCGACCGCGCGCGGCGTCGTGAAGCTGTCCGGCGAGCGCGCGGACAAGCGCG
TCGCGCACTGGCGCGGCGTCGTGCGCGCGTCGTGCGAACAATGCGGACGCAACCGCGTGCCCGACGTCGCACCGGTCGCC
GGCTTCAACGCCTGGCTCGATACGCTGCCGGCCGCACCGGCCGACGGCGAGCTGCGGCTGCTGCTGTCGCCGCGCGCGAG
CATCCCGTTCACGTCACTGCCCGACGCACCGCCTGCGGCAACCGTCACGCTGCTGATCGGACCGGAAGGCGGGCTGTCGC
CCGACGAGGAAAACGCGGCTCGCGCTCGCGGATTCACCGCACTGTCGCTCGGGCCCCGCGTGCTGCGCACCGAGACGGCC
GGCGCGGCCGTGCTCGCCGCGCTCGCGGCGCGCTGGGGCGGATGGTGA

Upstream 100 bases:

>100_bases
ATCAGTTCTGGGGTGACCGGTTCGCACAGATCGAGGATCTCGACGGCTACCGATGGGCGCTCGGGCGCCGCCTAGACGCA
TGACCCCAACCGTTACACGC

Downstream 100 bases:

>100_bases
CGCGCGACCGGGCACGCGTCGACGCATGACGCCGGCGCGCGTCAGCGGGCCGCGTTCAACGGCTCGATGTCGATGACCGG
AGCGCTCTGGAGCGCCGACA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNEATTTAAVPRFFVDAALRADATLALPADVARHAQVLRLQPGDVLALFDGTGGQYRARLVEIDKRSALAQIDTFEPAEA
EPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTARGVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVA
GFNAWLDTLPAAPADGELRLLLSPRASIPFTSLPDAPPAATVTLLIGPEGGLSPDEENAARARGFTALSLGPRVLRTETA
GAAVLAALAARWGGW

Sequences:

>Translated_255_residues
MNEATTTAAVPRFFVDAALRADATLALPADVARHAQVLRLQPGDVLALFDGTGGQYRARLVEIDKRSALAQIDTFEPAEA
EPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTARGVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVA
GFNAWLDTLPAAPADGELRLLLSPRASIPFTSLPDAPPAATVTLLIGPEGGLSPDEENAARARGFTALSLGPRVLRTETA
GAAVLAALAARWGGW
>Mature_255_residues
MNEATTTAAVPRFFVDAALRADATLALPADVARHAQVLRLQPGDVLALFDGTGGQYRARLVEIDKRSALAQIDTFEPAEA
EPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTARGVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVA
GFNAWLDTLPAAPADGELRLLLSPRASIPFTSLPDAPPAATVTLLIGPEGGLSPDEENAARARGFTALSLGPRVLRTETA
GAAVLAALAARWGGW

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=245, Percent_Identity=40.4081632653061, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26754; Mature: 26754

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEATTTAAVPRFFVDAALRADATLALPADVARHAQVLRLQPGDVLALFDGTGGQYRARL
CCCCHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHEEEECCCCEEEEEECCCCCEEEEE
VEIDKRSALAQIDTFEPAEAEPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTAR
EEEHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHEEEECCCCC
GVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVAGFNAWLDTLPAAPADGELRL
CEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEE
LLSPRASIPFTSLPDAPPAATVTLLIGPEGGLSPDEENAARARGFTALSLGPRVLRTETA
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCEEECCCCEEEEECCC
GAAVLAALAARWGGW
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MNEATTTAAVPRFFVDAALRADATLALPADVARHAQVLRLQPGDVLALFDGTGGQYRARL
CCCCHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHEEEECCCCEEEEEECCCCCEEEEE
VEIDKRSALAQIDTFEPAEAEPPYRVTLAQGIAGGDKMDWVIEKAVELGVAAVVPLSTAR
EEEHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCHHEEEECCCCC
GVVKLSGERADKRVAHWRGVVRASCEQCGRNRVPDVAPVAGFNAWLDTLPAAPADGELRL
CEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEE
LLSPRASIPFTSLPDAPPAATVTLLIGPEGGLSPDEENAARARGFTALSLGPRVLRTETA
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCEEECCCCEEEEECCC
GAAVLAALAARWGGW
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]