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The map label for this gene is btuE [H]

Identifier: 78050013

GI number: 78050013

Start: 5139348

End: 5139899

Strand: Reverse

Name: btuE [H]

Synonym: XCV4457

Alternate gene names: 78050013

Gene position: 5139899-5139348 (Counterclockwise)

Preceding gene: 78050014

Following gene: 78050012

Centisome position: 99.26

GC content: 65.94

Gene sequence:

>552_bases
ATGAGCCAGACGCTGTACGACATTCCCGTGACCCGCATCGACGGCCAGCCCACCACCCTGGCCGACTACCGCGGCAAGGT
GCTGCTGGTGGTCAACGTCGCCTCCAAGTGCGGCCTGACCCCGCAGTACGAAGGCCTGGAAGCGCTGTATCGCGACAAGC
GCATGCAAGGCCTGGAAGTGCTGGGGTTTCCGGCCAACGACTTCAACGCACAGGAGCCGGGCAGCGAAGCGGAAATCGCG
CAGTTCTGCCAGCTCACCTACGATGTCACCTTCCCGATGTTTGCCAAGATCGCCGTCACCGGCGACCAGGCGCACCCGCT
GTATCGCGCACTGACCAGCACGCACCCGCACACCACCGGCGACGGCCCGATGCGCGAAAAACTGGCCGGCTACGGCATCG
CCCCCAACCCCGCGCCTGGGGTGGTGTGGAACTTCGAAAAATTCCTGATCGGCCGCGACGGCAAGATCGTCGACCGCTTC
GCTCCGGACATCCCCGCCGACGATGCGCGCCTGCGTGCGGCAGTGGATGCGGCGCTGGCCACCCATGCATAG

Upstream 100 bases:

>100_bases
GGGCGTCGCTGCAGACAACCGCCAGCCGCGTGAGCCAGCGGCATCGCTCGCTCCACCCTGGCCGTTTATGCTTTCGGCTC
TACCTGTCCAGGACGCATCG

Downstream 100 bases:

>100_bases
CGCGCTGGCGGCATTGCGATGACGCGTCACGTTCTTATCGGGAGCCAGGGAACAGGCAATGACCTTCAATCACTTCTTCA
GCTCTTCAATGCTCGCGTTG

Product: glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MSQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRMQGLEVLGFPANDFNAQEPGSEAEIA
QFCQLTYDVTFPMFAKIAVTGDQAHPLYRALTSTHPHTTGDGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIVDRF
APDIPADDARLRAAVDAALATHA

Sequences:

>Translated_183_residues
MSQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRMQGLEVLGFPANDFNAQEPGSEAEIA
QFCQLTYDVTFPMFAKIAVTGDQAHPLYRALTSTHPHTTGDGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIVDRF
APDIPADDARLRAAVDAALATHA
>Mature_182_residues
SQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRMQGLEVLGFPANDFNAQEPGSEAEIAQ
FCQLTYDVTFPMFAKIAVTGDQAHPLYRALTSTHPHTTGDGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIVDRFA
PDIPADDARLRAAVDAALATHA

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=169, Percent_Identity=41.4201183431953, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI75709200, Length=162, Percent_Identity=38.8888888888889, Blast_Score=107, Evalue=4e-24,
Organism=Homo sapiens, GI90903240, Length=162, Percent_Identity=38.8888888888889, Blast_Score=107, Evalue=5e-24,
Organism=Homo sapiens, GI192455698, Length=169, Percent_Identity=33.1360946745562, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI90903238, Length=148, Percent_Identity=38.5135135135135, Blast_Score=94, Evalue=7e-20,
Organism=Homo sapiens, GI4557629, Length=164, Percent_Identity=34.1463414634146, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI6006001, Length=177, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=9e-18,
Organism=Homo sapiens, GI41406084, Length=156, Percent_Identity=37.1794871794872, Blast_Score=84, Evalue=5e-17,
Organism=Homo sapiens, GI33186887, Length=175, Percent_Identity=30.8571428571429, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI32967607, Length=175, Percent_Identity=31.4285714285714, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1788003, Length=182, Percent_Identity=46.1538461538462, Blast_Score=170, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17506887, Length=160, Percent_Identity=39.375, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17535473, Length=179, Percent_Identity=36.8715083798883, Blast_Score=116, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17550320, Length=170, Percent_Identity=34.7058823529412, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193209493, Length=168, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71993584, Length=165, Percent_Identity=32.7272727272727, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI193210707, Length=164, Percent_Identity=34.1463414634146, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71993573, Length=98, Percent_Identity=36.734693877551, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71999642, Length=78, Percent_Identity=38.4615384615385, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322228, Length=161, Percent_Identity=45.3416149068323, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319721, Length=161, Percent_Identity=42.2360248447205, Blast_Score=142, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322826, Length=159, Percent_Identity=36.4779874213836, Blast_Score=115, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24656767, Length=177, Percent_Identity=36.7231638418079, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24656777, Length=177, Percent_Identity=36.7231638418079, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24656772, Length=177, Percent_Identity=36.7231638418079, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI85726443, Length=163, Percent_Identity=36.8098159509202, Blast_Score=93, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 19921; Mature: 19790

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRMQGLEV
CCCCEECCCEEEECCCCCCHHCCCCCEEEEEEEHHHCCCCCCCHHHHHHHHHHHCCCCEE
LGFPANDFNAQEPGSEAEIAQFCQLTYDVTFPMFAKIAVTGDQAHPLYRALTSTHPHTTG
EECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCC
DGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIVDRFAPDIPADDARLRAAVDAALA
CCHHHHHHHCCCCCCCCCCCEEECHHHEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
THA
CCC
>Mature Secondary Structure 
SQTLYDIPVTRIDGQPTTLADYRGKVLLVVNVASKCGLTPQYEGLEALYRDKRMQGLEV
CCCEECCCEEEECCCCCCHHCCCCCEEEEEEEHHHCCCCCCCHHHHHHHHHHHCCCCEE
LGFPANDFNAQEPGSEAEIAQFCQLTYDVTFPMFAKIAVTGDQAHPLYRALTSTHPHTTG
EECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCC
DGPMREKLAGYGIAPNPAPGVVWNFEKFLIGRDGKIVDRFAPDIPADDARLRAAVDAALA
CCHHHHHHHCCCCCCCCCCCEEECHHHEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
THA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]