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The map label for this gene is degA [H]

Identifier: 78049888

GI number: 78049888

Start: 4978986

End: 4980059

Strand: Reverse

Name: degA [H]

Synonym: XCV4332

Alternate gene names: 78049888

Gene position: 4980059-4978986 (Counterclockwise)

Preceding gene: 78049895

Following gene: 78049884

Centisome position: 96.17

GC content: 66.39

Gene sequence:

>1074_bases
TTGGAGAAGGTCAGGAAATCGGTCCGCCGCACCAGCCGCGCGACCACCATCGACGAGGTGGCAGCGCTGGCAAAGGTCTC
GCCGATGACGGTCTCGCGCGTGGTCAACAACACCGGCAGCGTGCGCGATGCCACCCGCGAACGGGTCATGCGCGCGGTCG
ACAAGCTGGGCTACACGCCCAATCTTGCCGCCAGCGCACTGGCGGCCGCGCAGAGCACGCGGATTGCGTTGATCTACAGC
GATCCCAGCGGTGCCTACCTGCGCGAGTTGCTGCTGGGCGTGCTGCGGGTGGCATCGCGTACGTCGATCCAGCTGGTGAT
CGACTGCTGGGACGACCTGGACGCCGATGCCGAACGCAAGGCCGCGCGCAAGCTCGCCAAAGGCGTTGCCGGTGTGATCC
TGCCGCCGCCCTTGTGCGAATCCAGGGCCGCAGTGCTGGAATTGGTGCGCGCCAAGGTGCCGGTGGTGGCGATCGCCTCC
AACCATTTCAGCCCGGATGTGGCGTGTGTGCGCATCGACGAATTCGCTGCTGCCAAAGAGATCACCGAACATCTGATCGC
ACAGGGGCATGCGCGCATCGGCTACATCGCCGGGCATCCGAATCTGTCGGCCAGCACGCGGCGCTTCGAAGGCTTTCAGG
CGGCGCTGGCCGACGCCGGTCTGCGCCTGGATCGGCACTTGGTGCAGCCGGGCGACTACACCTACAAATCCGGGCTGGTT
GCCGCGGAAAAATTGCTGGCGCGCAAACGCCGTCCCAGCGCCATCTTCGCCAGCAACGACGACATGGCCGCCGCGGCAAT
CTCGGTCGCGCACCGGCGTGGGCTGGATGTGCCGCGCGATCTGTCGGTGGTCGGGTTCGACGACACCTCTGCCGCCACTG
CGGTGTGGCCCGAAATCACCACCGTGCAGCAGCCCATCGCCGCCATGGCCGATGCCGCGCTGGACATCTTGCTGAAGACG
ATTCGGGCCAAGGAGCGTACGGCAAGGATGGTCGATCATGTGGTCGCGCACCTGCTGGTCAAGCGGGATTCGGTGGCCGC
ACCGACAGCGGACGATGCGGCCGAGTCAACGTAG

Upstream 100 bases:

>100_bases
GTACCATGGCTCGCATCGTCATACCTGCACCCGGCGTCCGCGTTACCGCAGCAGCGCCCGTGCATCCCGCAGCTTTTGCA
CGCATTTGAAGAGGCCACGC

Downstream 100 bases:

>100_bases
CGATCAGACCCCTGCATGCCGCAAGCCGGATGTGGTCGCCTCGCAGGCTTGCATGCAACACACCGGCATTGCCGGTGTGC
GCTCACGTTTCTGGTGATCG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MEKVRKSVRRTSRATTIDEVAALAKVSPMTVSRVVNNTGSVRDATRERVMRAVDKLGYTPNLAASALAAAQSTRIALIYS
DPSGAYLRELLLGVLRVASRTSIQLVIDCWDDLDADAERKAARKLAKGVAGVILPPPLCESRAAVLELVRAKVPVVAIAS
NHFSPDVACVRIDEFAAAKEITEHLIAQGHARIGYIAGHPNLSASTRRFEGFQAALADAGLRLDRHLVQPGDYTYKSGLV
AAEKLLARKRRPSAIFASNDDMAAAAISVAHRRGLDVPRDLSVVGFDDTSAATAVWPEITTVQQPIAAMADAALDILLKT
IRAKERTARMVDHVVAHLLVKRDSVAAPTADDAAEST

Sequences:

>Translated_357_residues
MEKVRKSVRRTSRATTIDEVAALAKVSPMTVSRVVNNTGSVRDATRERVMRAVDKLGYTPNLAASALAAAQSTRIALIYS
DPSGAYLRELLLGVLRVASRTSIQLVIDCWDDLDADAERKAARKLAKGVAGVILPPPLCESRAAVLELVRAKVPVVAIAS
NHFSPDVACVRIDEFAAAKEITEHLIAQGHARIGYIAGHPNLSASTRRFEGFQAALADAGLRLDRHLVQPGDYTYKSGLV
AAEKLLARKRRPSAIFASNDDMAAAAISVAHRRGLDVPRDLSVVGFDDTSAATAVWPEITTVQQPIAAMADAALDILLKT
IRAKERTARMVDHVVAHLLVKRDSVAAPTADDAAEST
>Mature_357_residues
MEKVRKSVRRTSRATTIDEVAALAKVSPMTVSRVVNNTGSVRDATRERVMRAVDKLGYTPNLAASALAAAQSTRIALIYS
DPSGAYLRELLLGVLRVASRTSIQLVIDCWDDLDADAERKAARKLAKGVAGVILPPPLCESRAAVLELVRAKVPVVAIAS
NHFSPDVACVRIDEFAAAKEITEHLIAQGHARIGYIAGHPNLSASTRRFEGFQAALADAGLRLDRHLVQPGDYTYKSGLV
AAEKLLARKRRPSAIFASNDDMAAAAISVAHRRGLDVPRDLSVVGFDDTSAATAVWPEITTVQQPIAAMADAALDILLKT
IRAKERTARMVDHVVAHLLVKRDSVAAPTADDAAEST

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=344, Percent_Identity=33.1395348837209, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1790369, Length=350, Percent_Identity=31.1428571428571, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1789068, Length=338, Percent_Identity=31.0650887573964, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787948, Length=339, Percent_Identity=30.3834808259587, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=30.0970873786408, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788474, Length=334, Percent_Identity=32.9341317365269, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786540, Length=349, Percent_Identity=29.512893982808, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=28.9719626168224, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787580, Length=336, Percent_Identity=25.5952380952381, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1787906, Length=257, Percent_Identity=31.1284046692607, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789456, Length=325, Percent_Identity=26.1538461538462, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790715, Length=328, Percent_Identity=24.0853658536585, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786268, Length=312, Percent_Identity=25.9615384615385, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38248; Mature: 38248

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVRKSVRRTSRATTIDEVAALAKVSPMTVSRVVNNTGSVRDATRERVMRAVDKLGYTP
CHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
NLAASALAAAQSTRIALIYSDPSGAYLRELLLGVLRVASRTSIQLVIDCWDDLDADAERK
CHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
AARKLAKGVAGVILPPPLCESRAAVLELVRAKVPVVAIASNHFSPDVACVRIDEFAAAKE
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEHHHHHHHHH
ITEHLIAQGHARIGYIAGHPNLSASTRRFEGFQAALADAGLRLDRHLVQPGDYTYKSGLV
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHH
AAEKLLARKRRPSAIFASNDDMAAAAISVAHRRGLDVPRDLSVVGFDDTSAATAVWPEIT
HHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCCHH
TVQQPIAAMADAALDILLKTIRAKERTARMVDHVVAHLLVKRDSVAAPTADDAAEST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEKVRKSVRRTSRATTIDEVAALAKVSPMTVSRVVNNTGSVRDATRERVMRAVDKLGYTP
CHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
NLAASALAAAQSTRIALIYSDPSGAYLRELLLGVLRVASRTSIQLVIDCWDDLDADAERK
CHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
AARKLAKGVAGVILPPPLCESRAAVLELVRAKVPVVAIASNHFSPDVACVRIDEFAAAKE
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEHHHHHHHHH
ITEHLIAQGHARIGYIAGHPNLSASTRRFEGFQAALADAGLRLDRHLVQPGDYTYKSGLV
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHH
AAEKLLARKRRPSAIFASNDDMAAAAISVAHRRGLDVPRDLSVVGFDDTSAATAVWPEIT
HHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHCCCHH
TVQQPIAAMADAALDILLKTIRAKERTARMVDHVVAHLLVKRDSVAAPTADDAAEST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]