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The map label for this gene is spoT [H]

Identifier: 78049066

GI number: 78049066

Start: 4022390

End: 4024561

Strand: Reverse

Name: spoT [H]

Synonym: XCV3510

Alternate gene names: 78049066

Gene position: 4024561-4022390 (Counterclockwise)

Preceding gene: 78049067

Following gene: 78049065

Centisome position: 77.72

GC content: 64.83

Gene sequence:

>2172_bases
ATGAACCCAGGCCCCACTGCCCAGGCGACCGTCGTGACGTCTCCGTCTTCCGACCAGGCCATCCCCGACTACGTCCTGCA
TCTCGAGCGCGCGGCCAGCTACCTGCCCAAGGAGCAACTGCCGATCCTGCGCCGTGCCTGGGAAGTGGGCGCCAGCGCGC
ATGCCGGGCAGACCCGCAAGTCGGGCGAGCCCTACATCACCCACCCGGTGGCGGTGGCTGGCGTGCTGGCTGAGCTTGGC
CTGGACATGGAATCGCTGATCGCCGCAATCCTGCACGACACCATTGAAGATACGCCGCTGACGCGCGAAGAACTGGCGTC
GGAGTTCGGCGAGGCGGTGGCCGAGCTGGTCGATGGCGTCACCAAGCTGGACAAGCTCAAGTTCCGCGACCGCCAGGAAG
CGGCGGCAGAAAGTTTCCGCAAGATGCTGCTGGCGATGTCGCGCGACCTGCGCGTGATCATGATCAAGCTGGCCGACCGC
CTGCACAACATGCGCACGCTCGGCGCACAGAGCACCGAGGCGCGCGGCCGCATCGCGCGCGAGACGCTGGAGATCTACGC
ACCCATCGCGCAGCGGCTGGGCATGAGCCTGATCAAGTCCGAATTGCAGAATCTGGGCTTCCGCGCGTTGTATCCGTGGC
GCCACGCCATCATCGAAAAGCACATCCGCAGCCAGCCGGTGGTGCGGCGCGAATCGATGGCGCAGGTGGAAGTGCAGCTG
TCCCAGCGGCTGGCCAAGGAAGGGCTGGAGCATCGGCTGGTCAGCCGCATCAAGACGCCCTGGAGCATCTATTCCAAGAT
GCACGAAGAGAACAAGTCCTTCGACCAGGTGATGGACGTGTTCGGCTTTCGCCTGGTGGTGCGCACGGTGGCTGATTGCT
ATCACGCGCTGGGCGCGGTGCATGCCAGCTTCAAGCCGCTGGACGGGCGCTTCCGCGATTTCATCGCAATCCCCAAGGCC
AATGGCTACCAGTCGCTGCACACCGTGTTGTTCGGACCATACGGCTCGCCGATCGAAGTGCAGATACGCACCGAAGAGAT
GGACCTGATCGCCGAACGCGGCGTAGCGGCGCACTGGACCTACAAGGTCGGCTCGGCCTCGCCCAACAGCGCGCAGAGTC
GTGCGCACGACTGGATCGTGGAACTGATCGATTCGCAGCGCGCCGCCGGCTCGTCGCTGGAATTCCTGGACAACGTCAAG
GTCGACCTGTTCCCGGACGAGGTCTATCTGTTCACGCCCAAGGGCAAGATCCTGGCATTGCCGCGCAATTCCACCGCGCT
GGATTTTGCCTACGCGGTGCATACCGACGTGGGTAATCGCGCCGTGGCCTCGCGCGTGGACAAGAAGCTGGTGCCGTTGC
GCACCAAGCTGGTCAGCGGGCAGGCGGTGGAGATCATCACCGCCCGCTCGGCCACGCCCAAACCGCAGTGGCTGGAATTT
GTGGTCAGCAGCAAGGCGCGCACCGCCATCCGGCATCAGCTCAAGCAACTCGAGCACGAAGATGCCGTGCAGTTGGGCCA
CCGCATGCTGGACCGCGCGCTGGAAGCAATGGACTCTTCGCTGGAACGGCTGCCCAAGGGCCGCCTGGACGCCTTCCTTG
CCGAGCACCGCTACCCGCGTCTGGAAGCGCTGCTGGCCGATGTGGCGCTTGGCAACTGGATGCCCAATCAGGCCGCGCAG
GCGTTGATGGCTTACGCAGAACTGCGTGGCGGTGGCCTGTCCAAGCATTCGCACGAGAAGATCCTGATCGACGGCAGCGA
GCGCGGCGTGATCAGTTTCGCCAATTGCTGCCAGCCGATTCCCGGCGACGAAATCATGGGCTATCACACCGCAGGCAAGG
GCATCGTGGTGCACCGCCTGGACTGCCCGAACCTGGCGGAATTGCGCAAATCGCCCGAACGGTGGGTGCCGATCGACTGG
GACTCCAACGTGACCGGCGACTACGACACCGCGCTGGTGGTCGAAGTGGAAAACCGCACCGGCGTGCTGGCGCAGCTGGC
GGCAGCCATCGCGCAGAGTCAGTCCAATATCGAGCGCGTGGACTATCTGGACCGCGACTTCAACGCAGCCGTGCTGCGCT
TCAACATCCAGGTGCGCGACCGCCGCCACCTGGCCGAGGTGATGCGCCGGCTACGGCGTCTGCACGTGGTGCAGAGCGTC
GGACGCCAGTAG

Upstream 100 bases:

>100_bases
CAGCCCGCCTCGATGCGGGCTGTTTTCGTTTTCGGTCCCGGCAATACGCCTGCGTGTGCAAATGTTTGCCCAGACTGCAC
GGCTGGCATAGTCTTCCGGC

Downstream 100 bases:

>100_bases
CGCCTGGCGCACTCGCGCAACAGTCGAGCAAATCCGCACAAAGGCCTAAACTGCGGTAACCCTTTGCAGTGGAGTGTCCA
TGTCCCAGATCATCCATACC

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGASAHAGQTRKSGEPYITHPVAVAGVLAELG
LDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGVTKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSTEARGRIARETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAVHASFKPLDGRFRDFIAIPKA
NGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWTYKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVK
VDLFPDEVYLFTPKGKILALPRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHRYPRLEALLADVALGNWMPNQAAQ
ALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPIPGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDW
DSNVTGDYDTALVVEVENRTGVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
GRQ

Sequences:

>Translated_723_residues
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGASAHAGQTRKSGEPYITHPVAVAGVLAELG
LDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGVTKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSTEARGRIARETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAVHASFKPLDGRFRDFIAIPKA
NGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWTYKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVK
VDLFPDEVYLFTPKGKILALPRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHRYPRLEALLADVALGNWMPNQAAQ
ALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPIPGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDW
DSNVTGDYDTALVVEVENRTGVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
GRQ
>Mature_723_residues
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGASAHAGQTRKSGEPYITHPVAVAGVLAELG
LDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGVTKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADR
LHNMRTLGAQSTEARGRIARETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAVHASFKPLDGRFRDFIAIPKA
NGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWTYKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVK
VDLFPDEVYLFTPKGKILALPRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHRYPRLEALLADVALGNWMPNQAAQ
ALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPIPGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDW
DSNVTGDYDTALVVEVENRTGVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
GRQ

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=137, Percent_Identity=37.2262773722628, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1790082, Length=695, Percent_Identity=48.0575539568345, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=687, Percent_Identity=33.0422125181951, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25143118, Length=129, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 80833; Mature: 80833

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGASAHAGQTRK
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCCCCCCCCCC
SGEPYITHPVAVAGVLAELGLDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGV
CCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
TKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLGAQSTEARGRIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HASFKPLDGRFRDFIAIPKANGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWT
HHCCCCCCCCHHHEEECCCCCCHHHHHHHEECCCCCCEEEEEEHHHHHHHHHCCCEEEEE
YKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVKVDLFPDEVYLFTPKGKILAL
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCEEEECCCCCEEEE
PRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHRYPR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
LEALLADVALGNWMPNQAAQALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCC
PGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDWDSNVTGDYDTALVVEVENRT
CCCCEEEEEECCCCEEEEECCCCCHHHHHCCCCCEEECCCCCCCCCCCCCEEEEEECCCC
GVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
GRQ
CCC
>Mature Secondary Structure
MNPGPTAQATVVTSPSSDQAIPDYVLHLERAASYLPKEQLPILRRAWEVGASAHAGQTRK
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHCCCCCCCCCCC
SGEPYITHPVAVAGVLAELGLDMESLIAAILHDTIEDTPLTREELASEFGEAVAELVDGV
CCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
TKLDKLKFRDRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLGAQSTEARGRIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ETLEIYAPIAQRLGMSLIKSELQNLGFRALYPWRHAIIEKHIRSQPVVRRESMAQVEVQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SQRLAKEGLEHRLVSRIKTPWSIYSKMHEENKSFDQVMDVFGFRLVVRTVADCYHALGAV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HASFKPLDGRFRDFIAIPKANGYQSLHTVLFGPYGSPIEVQIRTEEMDLIAERGVAAHWT
HHCCCCCCCCHHHEEECCCCCCHHHHHHHEECCCCCCEEEEEEHHHHHHHHHCCCEEEEE
YKVGSASPNSAQSRAHDWIVELIDSQRAAGSSLEFLDNVKVDLFPDEVYLFTPKGKILAL
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCEEEECCCCCEEEE
PRNSTALDFAYAVHTDVGNRAVASRVDKKLVPLRTKLVSGQAVEIITARSATPKPQWLEF
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
VVSSKARTAIRHQLKQLEHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHRYPR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
LEALLADVALGNWMPNQAAQALMAYAELRGGGLSKHSHEKILIDGSERGVISFANCCQPI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEHHHHCCCC
PGDEIMGYHTAGKGIVVHRLDCPNLAELRKSPERWVPIDWDSNVTGDYDTALVVEVENRT
CCCCEEEEEECCCCEEEEECCCCCHHHHHCCCCCEEECCCCCCCCCCCCCEEEEEECCCC
GVLAQLAAAIAQSQSNIERVDYLDRDFNAAVLRFNIQVRDRRHLAEVMRRLRRLHVVQSV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
GRQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]