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The map label for this gene is pykA [H]

Identifier: 78049019

GI number: 78049019

Start: 3965722

End: 3967188

Strand: Reverse

Name: pykA [H]

Synonym: XCV3463

Alternate gene names: 78049019

Gene position: 3967188-3965722 (Counterclockwise)

Preceding gene: 78049020

Following gene: 78049018

Centisome position: 76.61

GC content: 65.1

Gene sequence:

>1467_bases
ATGAAAGAACGTCAACGCCGCACCAAGATCCTCGCCACCCTCGGACCGGCAACGGATGCGCCCGGTGTGCTGGATACGCT
GTTCAAGGCGGGCGTCAACGTGGTGCGCCTCAACTTCAGCCATGGCGACCCCTCTGGCCAGGCCAAGCGCGCCGCCGAAG
TACGTGCCGCTGCAGCCCGCGTCGGTGCCGAGGTCGGCATCCTGGCCGACTTGCCAGGCCCGAAGATCCGTATCGAACGG
TTTGCCGAAGGCAAGATCAAGCTGACCATGGGCGATCGCTTCGACCTGGTGGCCGATGCCAACGCGCCGGCTGGCGACCA
GACGCAGGTTGGCGTCAGCTATCTGGGGCTGCCGCAGGACGTGACCGCCGGCGACGTGCTGCTGCTCGACGATGGCCTGA
TGCAGTTGCAGGTGACCGATGTGCAGGGCGAGCGCATCGTCACCAAGGTGTTGAACGATGGTGTGCTGTCCGACCGCAAG
GGCCTCAACAAGCAGGGCGGTGGTCTGTCGCTGGGCGCGTTGACCGAGCGCGACAAGGAACTGATCGGCATCGTGGCCAA
GATCGGCGTGGACTTCATTGCGGTGTCGTTCTGTCGCAATGCGCAGGACATGCACGACGCGCGTCAGATCGCCCAGCAGC
ACGGCTGCGATGCGCAACTGGTGTCCAAGATCGAGCGCACCGAGGCGATCGAAAACCTGGTCGAGATCGTCGAAGCCTCC
GATGTGGTGATGGTGGCGCGTGGCGATCTGGGCGTTGAGATCGGTGACGCTGAATTGCCCGGCCTGCAGAAGAAGATCAT
TCGCGAATCGCTGGCGCAGAATAAGGTGGTGATCACTGCCACGCAGATGCTGCAGTCGATGGTCGAAAGCCCGATCCCTA
CCCGCGCCGAAGTGCTGGACGTGGCCAACGCGGTCATCGACGGTACCGATGCGGTGATGCTCTCGGCCGAGACCGCCGCC
GGCGCCTATCCGGTGCGTGCGGTGGAAGCGATGGCGCGCATCTGCCTGGGGGCCGAACACCAGTTCGAATTCGACACCGA
TTTCGAAGCGGCGCAACGCAATCTGCAGCGCGCCGACCAAGCGATTGCGATGGCAACCATGTTCCTGTCCGAGCACATCG
GCCTGGGCGGCGTGGTGGCGTTGACCGAATCCGGCGGCACGCCGCGCTTCCTGTCGCGCTTCCGCTCCAATATGCCGATC
TACGCCTTCACCCGTCACGATGGCGCGCGCCGCCAGATGGCCATGATGCGTGGCGTGTTCCCGATCAGCTTCGACAGCCG
CGGCCTGACCCCGCGCGAAGCCGCGCGTGCGGCGATCCGTCTGCTGGTGGAGAACGAGCGCATGGGGCCTGGCGACCGCG
TGGTCTTTACCAGTGGCGAGCACATGGAAACCCATGGCGCCACCAACACGCTGCGCCTGCTGGAAGTGGGCGAAGACGGT
CGCGCCACCGGGCTGGGCGAGCTCTAA

Upstream 100 bases:

>100_bases
TCTATAAGACATGCCGGAATCGGAGGAAGGCTATGCCGCAATGCCATCCTCGGTACGTGACGTGTGTGTTAAGTTTCGCG
GCTTTTACAGGGAGGCCATC

Downstream 100 bases:

>100_bases
CGCCTGTTGATTGCATCCGGCGCATGACCTGAGCCGGAACAGAGGCGACACACGACGGCGGCCCCAGGGCCGCCGTCGTG
CTGTGTGGCGACCTGGCCGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKERQRRTKILATLGPATDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAARVGAEVGILADLPGPKIRIER
FAEGKIKLTMGDRFDLVADANAPAGDQTQVGVSYLGLPQDVTAGDVLLLDDGLMQLQVTDVQGERIVTKVLNDGVLSDRK
GLNKQGGGLSLGALTERDKELIGIVAKIGVDFIAVSFCRNAQDMHDARQIAQQHGCDAQLVSKIERTEAIENLVEIVEAS
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSAETAA
GAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQAIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPI
YAFTRHDGARRQMAMMRGVFPISFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGEDG
RATGLGEL

Sequences:

>Translated_488_residues
MKERQRRTKILATLGPATDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAARVGAEVGILADLPGPKIRIER
FAEGKIKLTMGDRFDLVADANAPAGDQTQVGVSYLGLPQDVTAGDVLLLDDGLMQLQVTDVQGERIVTKVLNDGVLSDRK
GLNKQGGGLSLGALTERDKELIGIVAKIGVDFIAVSFCRNAQDMHDARQIAQQHGCDAQLVSKIERTEAIENLVEIVEAS
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSAETAA
GAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQAIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPI
YAFTRHDGARRQMAMMRGVFPISFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGEDG
RATGLGEL
>Mature_488_residues
MKERQRRTKILATLGPATDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAARVGAEVGILADLPGPKIRIER
FAEGKIKLTMGDRFDLVADANAPAGDQTQVGVSYLGLPQDVTAGDVLLLDDGLMQLQVTDVQGERIVTKVLNDGVLSDRK
GLNKQGGGLSLGALTERDKELIGIVAKIGVDFIAVSFCRNAQDMHDARQIAQQHGCDAQLVSKIERTEAIENLVEIVEAS
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSAETAA
GAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQAIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPI
YAFTRHDGARRQMAMMRGVFPISFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGEDG
RATGLGEL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=430, Percent_Identity=36.5116279069767, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI33286420, Length=430, Percent_Identity=36.5116279069767, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=34.8884381338742, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=35.4969574036511, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=35.4969574036511, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=34.1463414634146, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=34.1463414634146, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=31.4893617021277, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=31.4893617021277, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=30.8411214953271, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=51.25, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1787965, Length=467, Percent_Identity=37.9014989293362, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=34.7826086956522, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=490, Percent_Identity=35.7142857142857, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=490, Percent_Identity=35.7142857142857, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI71984413, Length=490, Percent_Identity=35.7142857142857, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI71984406, Length=490, Percent_Identity=35.7142857142857, Blast_Score=238, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=434, Percent_Identity=36.1751152073733, Blast_Score=259, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=34.2857142857143, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24648964, Length=488, Percent_Identity=36.0655737704918, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI28571814, Length=488, Percent_Identity=36.0655737704918, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI24648966, Length=406, Percent_Identity=35.2216748768473, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24581235, Length=430, Percent_Identity=30.4651162790698, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24646914, Length=283, Percent_Identity=37.1024734982332, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52327; Mature: 52327

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERQRRTKILATLGPATDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAAR
CCCHHHHHHHHHEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
VGAEVGILADLPGPKIRIERFAEGKIKLTMGDRFDLVADANAPAGDQTQVGVSYLGLPQD
HCCCEEEEECCCCCCEEEEEECCCEEEEEECCCEEEEECCCCCCCCCCCCCHHEECCCCC
VTAGDVLLLDDGLMQLQVTDVQGERIVTKVLNDGVLSDRKGLNKQGGGLSLGALTERDKE
CCCCCEEEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHH
LIGIVAKIGVDFIAVSFCRNAQDMHDARQIAQQHGCDAQLVSKIERTEAIENLVEIVEAS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLD
CEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHH
VANAVIDGTDAVMLSAETAAGAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQ
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPIYAFTRHDGARRQMAMMRGVF
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCC
PISFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGEDG
CEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEECCCCCCEEEEEEECCCC
RATGLGEL
CCCCCCCC
>Mature Secondary Structure
MKERQRRTKILATLGPATDAPGVLDTLFKAGVNVVRLNFSHGDPSGQAKRAAEVRAAAAR
CCCHHHHHHHHHEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
VGAEVGILADLPGPKIRIERFAEGKIKLTMGDRFDLVADANAPAGDQTQVGVSYLGLPQD
HCCCEEEEECCCCCCEEEEEECCCEEEEEECCCEEEEECCCCCCCCCCCCCHHEECCCCC
VTAGDVLLLDDGLMQLQVTDVQGERIVTKVLNDGVLSDRKGLNKQGGGLSLGALTERDKE
CCCCCEEEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHH
LIGIVAKIGVDFIAVSFCRNAQDMHDARQIAQQHGCDAQLVSKIERTEAIENLVEIVEAS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVVMVARGDLGVEIGDAELPGLQKKIIRESLAQNKVVITATQMLQSMVESPIPTRAEVLD
CEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCHHHHHH
VANAVIDGTDAVMLSAETAAGAYPVRAVEAMARICLGAEHQFEFDTDFEAAQRNLQRADQ
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AIAMATMFLSEHIGLGGVVALTESGGTPRFLSRFRSNMPIYAFTRHDGARRQMAMMRGVF
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCC
PISFDSRGLTPREAARAAIRLLVENERMGPGDRVVFTSGEHMETHGATNTLRLLEVGEDG
CEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEECCCCCCEEEEEEECCCC
RATGLGEL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]