Click here to switch to the map view.

The map label for this gene is cysB [H]

Identifier: 78049013

GI number: 78049013

Start: 3959075

End: 3960058

Strand: Reverse

Name: cysB [H]

Synonym: XCV3457

Alternate gene names: 78049013

Gene position: 3960058-3959075 (Counterclockwise)

Preceding gene: 78049018

Following gene: 78049012

Centisome position: 76.47

GC content: 68.5

Gene sequence:

>984_bases
ATGACGCTGACCCAACTTCGCTATCTGGTGGCCATTGCCGATGCCGAGCTGAACATCACCCTGGCCGCCGCGCGCGTGCA
CGCGACCCAGCCCGGCCTGTCCAAGCAGCTCAAGCAGCTGGAGGACGAACTGGGCTTTCTGCTGTTCGTGCGCAAGGGAC
GTAGCCTGGACGCAGTGACGCCGGCCGGTGTGGAAGTGATCGAGCGCGCGCGCGCGGTGCTGTCGGAGGCCAACAACATC
CGCACCTATGCGGCCAACCAGCGCCGCGAAAGCCAGGGCCAGCTGACCCTGACCACCACCCATACCCAGGCGCGCTTCGT
GCTGCCGCCGGCGGTGGCACAGATCAAGAATGCCTACCCGCAAGTGAGCGTGCATCTGCAGCAGGCCGCCGAAAGTGCGG
CCCTGGATCTGCTGAGCCAGGGCGATGCGGACATCGCGGTGGTCAGCACCGCCGGTGGCGAGCCGAGCGCCGGCATCGCA
GTGCCGCTGTACCGCTGGCGGCGGCTGGTGGTGGTGCCGCGGGGGCATGCACTGGATCACCCGCGCACGGCACCGGACAT
GCAGGCGCTGGCCGAGCATCCGCTGATCAGCTACGAATCCTCCACCCGGCCGGGCTCGTCGCTGCAGCGCGCGTTTGCGC
AGGTGGGGCTGGAGCCGAGCATTGCGCTGACCGCGCTGGATGCCGACCTGATCAAGACCTATGTGCGCGCAGGGCTTGGC
GTGGGATTGGTCGCGGAAATGGCGGTCAATGCCTTCGACGAGGACCTGCGCGCCTGGCCGGCGCCGGCGCCGATTGCCGA
GTGCATCGCCTGGGCGGTGCTGCCGCGCGACCGCGTGCTGCGCGACTACGCGCTGGACCTGGTGCATGTGCTCGCGCCGC
AAATCGACAAGCGCGACCTACGCCGCGTGCTGGACGGCAATCAGGCGCCCAACTGGCCGTTGCCGCCGACCTGGGAATCG
TTGACCCAGACCATCACCAGTTGA

Upstream 100 bases:

>100_bases
TTCATCCATCCTAAAAATAGCGTTCGGTTATAAGCTGGCCCGCAGAACTCCTCTACAGTCGAAGTCCTTGTAGCGCCTCG
CGCTTTTTTGGATATAAGCC

Downstream 100 bases:

>100_bases
GCGGCACGATGACCGAGCCCTTGCGCCCCGCACTGAGCAGGCTGTGGTCGTCCGAGCCGGACGGCGGCATGTCGCTGCAG
CTGTCGGCCAGCATCGCCGG

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTLTQLRYLVAIADAELNITLAAARVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDAVTPAGVEVIERARAVLSEANNI
RTYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYPQVSVHLQQAAESAALDLLSQGDADIAVVSTAGGEPSAGIA
VPLYRWRRLVVVPRGHALDHPRTAPDMQALAEHPLISYESSTRPGSSLQRAFAQVGLEPSIALTALDADLIKTYVRAGLG
VGLVAEMAVNAFDEDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDLRRVLDGNQAPNWPLPPTWES
LTQTITS

Sequences:

>Translated_327_residues
MTLTQLRYLVAIADAELNITLAAARVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDAVTPAGVEVIERARAVLSEANNI
RTYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYPQVSVHLQQAAESAALDLLSQGDADIAVVSTAGGEPSAGIA
VPLYRWRRLVVVPRGHALDHPRTAPDMQALAEHPLISYESSTRPGSSLQRAFAQVGLEPSIALTALDADLIKTYVRAGLG
VGLVAEMAVNAFDEDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDLRRVLDGNQAPNWPLPPTWES
LTQTITS
>Mature_326_residues
TLTQLRYLVAIADAELNITLAAARVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDAVTPAGVEVIERARAVLSEANNIR
TYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYPQVSVHLQQAAESAALDLLSQGDADIAVVSTAGGEPSAGIAV
PLYRWRRLVVVPRGHALDHPRTAPDMQALAEHPLISYESSTRPGSSLQRAFAQVGLEPSIALTALDADLIKTYVRAGLGV
GLVAEMAVNAFDEDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDLRRVLDGNQAPNWPLPPTWESL
TQTITS

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=301, Percent_Identity=39.202657807309, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788296, Length=311, Percent_Identity=32.475884244373, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI2367136, Length=244, Percent_Identity=28.6885245901639, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790399, Length=252, Percent_Identity=28.1746031746032, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787806, Length=177, Percent_Identity=31.0734463276836, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=28.7671232876712, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI157672245, Length=226, Percent_Identity=26.9911504424779, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787879, Length=300, Percent_Identity=24.6666666666667, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=239, Percent_Identity=29.7071129707113, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35491; Mature: 35360

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTQLRYLVAIADAELNITLAAARVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDAVT
CCHHHHHHHHEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCC
PAGVEVIERARAVLSEANNIRTYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYP
CHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEECCCEEEECCHHHHHHHHCCC
QVSVHLQQAAESAALDLLSQGDADIAVVSTAGGEPSAGIAVPLYRWRRLVVVPRGHALDH
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEHHHCEEEEEEECCCCCCC
PRTAPDMQALAEHPLISYESSTRPGSSLQRAFAQVGLEPSIALTALDADLIKTYVRAGLG
CCCCHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCC
VGLVAEMAVNAFDEDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
RRVLDGNQAPNWPLPPTWESLTQTITS
HHHHCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TLTQLRYLVAIADAELNITLAAARVHATQPGLSKQLKQLEDELGFLLFVRKGRSLDAVT
CHHHHHHHHEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCC
PAGVEVIERARAVLSEANNIRTYAANQRRESQGQLTLTTTHTQARFVLPPAVAQIKNAYP
CHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEECCCEEEECCHHHHHHHHCCC
QVSVHLQQAAESAALDLLSQGDADIAVVSTAGGEPSAGIAVPLYRWRRLVVVPRGHALDH
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEHHHCEEEEEEECCCCCCC
PRTAPDMQALAEHPLISYESSTRPGSSLQRAFAQVGLEPSIALTALDADLIKTYVRAGLG
CCCCHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCC
VGLVAEMAVNAFDEDLRAWPAPAPIAECIAWAVLPRDRVLRDYALDLVHVLAPQIDKRDL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHH
RRVLDGNQAPNWPLPPTWESLTQTITS
HHHHCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]