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The map label for this gene is yfiF [H]

Identifier: 78048990

GI number: 78048990

Start: 3925445

End: 3926617

Strand: Reverse

Name: yfiF [H]

Synonym: XCV3434

Alternate gene names: 78048990

Gene position: 3926617-3925445 (Counterclockwise)

Preceding gene: 78048991

Following gene: 78048989

Centisome position: 75.83

GC content: 68.88

Gene sequence:

>1173_bases
ATGGCCGCCGCAGGAGTCTTGCAGATGCTGCGCGCAGTCGGCCACCTCAACCTCGCCCCCGCAGAGCGGCGCAACGTTCA
ACATGCTTCGGCGCAGCCGCCATGTACGGACCATCGCGCCGGTGGCTTACCATCGGCGTCACTGCCTGTGCGGCAGCGGC
CTTACCGGTACTGCAACACGATCGACACGCAGACGGCGAAACGGCCGGAAGCAGCCGTTCCGCACTGCAAGCAACTCGAA
CTCCTGGATCCGACAATGACACAGCATGATGGACGCGGCGGACGGCCGCCACGCGACGGCGGCTCTGGCCCCTCTCACAA
CCCGTGGGGCCGCGCTGCGCCGCGCACACCTGCACCGCGCAGTGCCGCACCCCGCGCTCCAGCGCAGCACACTTCCGCTG
TCACGCCACCTGCCGCTGCAGCAAGCGGCAACGCGCGTGAGGTGCGGCTGTACGGCATCAACGCCGTGCAGGCCGTGTTC
AAGGCGCGCCCGCAGGCGCTGCGCAAGCTGTACCTAAGCGAGGCGCGCATTCCGCAGTTCAAGGCGCTGCTGGCCTGGTG
CGTGACCCAGCGCATCGGCTATCGGGTGGTGGACGAGGCCGATCTGAGCAAGCTCGCTGCCAGTGCGCACCATGAAGGCA
TCGTCGCCGAGGTGCTGCGCGTCGCCCCGCAACCGTTGCAGGAATGGCTGGCAGCGTTGCCGCAGGGCCCGGTGCTGGCG
CTGTGGCTGGACGGGGTGGGCAACCCGCACAACTTCGGGGCGATATTGCGCTCGTCGGCGCACTTCGGTGTGGCCGGTCT
GTTGTTGCCGGCCGGCTCCACGTTGGGCCTGTCGGGCGCGGCGGCACGCGTGGCCGAAGGCGGCGCCGAAGCGGTACCGC
TGGTGCAACTGCCCGACACTGCAACGGCGATGGCGCAGCTGCGCCAGGCCGGTTTTGCGATTGCCGCGACCTTGGTCGAT
GGTGGCCAGGACGTCTTCGCCGCCGCGCTGCCGCAGCGCCTGGTGTATGTGATGGGCGCGGAAAGCGAAGGCATGGACCG
CGCCTTCGCGCGCGATTGCGATCTGCAGTTGTCGATCCGCGGCAGCGGCAAGGTGGAAAGTCTCAACGTCGCCTCGGCCA
CTGCGGTCTTCCAGGCCGCCTGGCACGCGCGCGTGCTCAACGGAGAACACTGA

Upstream 100 bases:

>100_bases
TGTTCGCGCGGGTGAATCCACCCGTTGCGGTTGGCGTGCCGGTGTGCACATCCGCCCCTGCGTGATTGCATGCACATGAT
GGCCAGCTGCAGGACTGGAA

Downstream 100 bases:

>100_bases
TGGTGCGTTTTTGTGCGTTGTTGCTTGCGACCGGTTGCCTGTTCAGTGCCAACGCGTCTGCCGCCACGCCGGCGGCACCT
CCCGAGGTGCGCACCGATCA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MAAAGVLQMLRAVGHLNLAPAERRNVQHASAQPPCTDHRAGGLPSASLPVRQRPYRYCNTIDTQTAKRPEAAVPHCKQLE
LLDPTMTQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPRSAAPRAPAQHTSAVTPPAAAASGNAREVRLYGINAVQAVF
KARPQALRKLYLSEARIPQFKALLAWCVTQRIGYRVVDEADLSKLAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLA
LWLDGVGNPHNFGAILRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATAMAQLRQAGFAIAATLVD
GGQDVFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARVLNGEH

Sequences:

>Translated_390_residues
MAAAGVLQMLRAVGHLNLAPAERRNVQHASAQPPCTDHRAGGLPSASLPVRQRPYRYCNTIDTQTAKRPEAAVPHCKQLE
LLDPTMTQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPRSAAPRAPAQHTSAVTPPAAAASGNAREVRLYGINAVQAVF
KARPQALRKLYLSEARIPQFKALLAWCVTQRIGYRVVDEADLSKLAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLA
LWLDGVGNPHNFGAILRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATAMAQLRQAGFAIAATLVD
GGQDVFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARVLNGEH
>Mature_389_residues
AAAGVLQMLRAVGHLNLAPAERRNVQHASAQPPCTDHRAGGLPSASLPVRQRPYRYCNTIDTQTAKRPEAAVPHCKQLEL
LDPTMTQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPRSAAPRAPAQHTSAVTPPAAAASGNAREVRLYGINAVQAVFK
ARPQALRKLYLSEARIPQFKALLAWCVTQRIGYRVVDEADLSKLAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLAL
WLDGVGNPHNFGAILRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATAMAQLRQAGFAIAATLVDG
GQDVFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARVLNGEH

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=316, Percent_Identity=35.126582278481, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1790623, Length=244, Percent_Identity=34.8360655737705, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 41000; Mature: 40869

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAGVLQMLRAVGHLNLAPAERRNVQHASAQPPCTDHRAGGLPSASLPVRQRPYRYCNT
CCHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
IDTQTAKRPEAAVPHCKQLELLDPTMTQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPR
CCCHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SAAPRAPAQHTSAVTPPAAAASGNAREVRLYGINAVQAVFKARPQALRKLYLSEARIPQF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCHHHHHHHHHHHCCCHHH
KALLAWCVTQRIGYRVVDEADLSKLAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLA
HHHHHHHHHHHHCCEEECHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCEEE
LWLDGVGNPHNFGAILRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDT
EEECCCCCCCHHHHHHHCCCCCCHHHHEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCH
ATAMAQLRQAGFAIAATLVDGGQDVFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIR
HHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHCCCCEEEEEC
GSGKVESLNVASATAVFQAAWHARVLNGEH
CCCCEEEECHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AAAGVLQMLRAVGHLNLAPAERRNVQHASAQPPCTDHRAGGLPSASLPVRQRPYRYCNT
CHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
IDTQTAKRPEAAVPHCKQLELLDPTMTQHDGRGGRPPRDGGSGPSHNPWGRAAPRTPAPR
CCCHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SAAPRAPAQHTSAVTPPAAAASGNAREVRLYGINAVQAVFKARPQALRKLYLSEARIPQF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCHHHHHHHHHHHCCCHHH
KALLAWCVTQRIGYRVVDEADLSKLAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLA
HHHHHHHHHHHHCCEEECHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCEEE
LWLDGVGNPHNFGAILRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDT
EEECCCCCCCHHHHHHHCCCCCCHHHHEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCH
ATAMAQLRQAGFAIAATLVDGGQDVFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIR
HHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHCCCCEEEEEC
GSGKVESLNVASATAVFQAAWHARVLNGEH
CCCCEEEECHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]