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The map label for this gene is nadE [H]

Identifier: 78048901

GI number: 78048901

Start: 3830291

End: 3831934

Strand: Reverse

Name: nadE [H]

Synonym: XCV3345

Alternate gene names: 78048901

Gene position: 3831934-3830291 (Counterclockwise)

Preceding gene: 78048902

Following gene: 78048898

Centisome position: 74.0

GC content: 66.18

Gene sequence:

>1644_bases
GTGACAGCACACACCCTCCGCATCGCCATGGCCCAGTTCGACTTCCCGGTCGGCGGCGTGACGCAGAATACCGATCGCAT
CATCGAGTACATCGCAGCGGCGCGCGACGAGTTCGAGGCCGACATCGTGCTGTTCCCCGAATTGGCGATCAGCGGGTATC
CGCCGGAAGACCTGCTGTTACGACCGGGATTCCTCGCTCATTGCGAACAGGCGCTGGCGCGGATTGCGGCGGCCACGCGC
GGCATCGTGGCAGTGGTCGGCTGGCCGCAAAGCGCCGGCAGCGTGGTCTACAACGCCGCCAGCGTGTTGCGCGACGGGCG
GATCGAAGCCACCTACCGCAAGCGCGAATTGCCCAACTATGCGGTGTTCGACGAGCGTCGTTATTTCGACGTGGATCCGG
ATGGCGAGAGCTGCGTGGTCACGGTCAAGGGCGTGCAGGTGGGCGTGGTGATCTGCGAAGACCTCTGGTTCGCCGAGCCC
TTGGCCAACACGGTACGGGCGGGCGCGGAGTTGGTGCTGGTGCCCAATGCCTCGCCGTACGAGCGTGGCAAGCATGCCCA
ACGCGATGCATTGCTGGCCGAACGCACCCGCGAGAGCGGGGCAGCGATTGCCTATCTCAACGTCGTGGGCGGGCAGGATG
CACTGGTGTTCGATGGCGCCTCGGTGGTGGCCGATGGCGACGGCACCGTGCATCCCGCCGCTGCAGCCTTCGTGGACCAG
TGGCTGGTGGTGGACTATGCGGCCGGCGAGCGCAGCTTCACTCCGGTGGTGTGGGTGGATGACGGCGACGAAAGCATGGA
TGCGCTGGCCTGGCGTGCGGTGGTGCGCGGGCTGCAGGACTATTGCCGCAAGAACGGCTTCAGCAAGGTCTGGCTTGGCT
TGTCCGGCGGCATCGACTCGGCACTGGTGTTGGCGATGGCGGTGGATGCGCTGGGCGGCGACAACGTGACCGCCGTGCGC
CTGCCGTCGCGCTACACCGCCACCCTGTCCAACGACCTGGCCGACGAGCAATGCCGCGCGCTGGGGGTGAAGCTGGAGAC
CATCGCGATCGAACCGGCTTTCGAAGGCCTGCTCGGCGCGCTGGGGCCGATGTTCGAAGGCACCCAGCCGGACATCACCG
AAGAAAACCTGCAGTCGCGCAGCCGTGGCGTGATCCTGATGGCGCTGTCGAACAAGTTCGGCGGGCTGGTGCTGACCACC
GGTAACAAGAGCGAATACGCAGTGGGTTACGCCACCATCTATGGCGACATGTGCGGTGGCTATGCGCCGCTCAAGGACCT
TTACAAGACGGAGGTGTTCGGCCTGGCGAAATGGCGCAACACCGTCGGCGGCGCGCCGGTGATTCCGCCGGCGGTGATCT
CGCGCCCGCCGTCGGCCGAGTTGCGCGCCAACCAGACCGACCAGGATTCGCTGCCGCCGTACGATGTGCTCGACGGCATC
CTGTATCGCTACGTCGACCAGGAGCAGTCGCGCGACGACATCGTTGCGGCTGGCTACGCGGCCGATACCGTCGAGCATGT
GCTGCGCCTGGTGCGGCTCAACGAGTGGAAGCGCCATCAGTCCGCGCCCGGGCCCAAGGTATCGCGGCGTGCATTCGGTC
GCGAACGGCGCTATCCGATCACCAACGGGTATCGCGGGCAGTAA

Upstream 100 bases:

>100_bases
TCTATCGTTACGCACCGTTGGACGACACCGTGTACGTGCTGGCGATCCGTTCGCAGCGCGAAGCGGGTTACCTTGAAAGC
CTGTTTTGATGGAGTCAGCA

Downstream 100 bases:

>100_bases
TCCGGACGGCTAGTCTGGTCACTCGGCTGACGCAGTGGCTGCCGCTGCGCTGGCTGGCGGCTGGGCGAACCGCGTGAGCA
GATCCAGGGGCGGTTTTGGT

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLRPGFLAHCEQALARIAAATR
GIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYAVFDERRYFDVDPDGESCVVTVKGVQVGVVICEDLWFAEP
LANTVRAGAELVLVPNASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQ
WLVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDSALVLAMAVDALGGDNVTAVR
LPSRYTATLSNDLADEQCRALGVKLETIAIEPAFEGLLGALGPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTT
GNKSEYAVGYATIYGDMCGGYAPLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRANQTDQDSLPPYDVLDGI
LYRYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPITNGYRGQ

Sequences:

>Translated_547_residues
MTAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLRPGFLAHCEQALARIAAATR
GIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYAVFDERRYFDVDPDGESCVVTVKGVQVGVVICEDLWFAEP
LANTVRAGAELVLVPNASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQ
WLVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDSALVLAMAVDALGGDNVTAVR
LPSRYTATLSNDLADEQCRALGVKLETIAIEPAFEGLLGALGPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTT
GNKSEYAVGYATIYGDMCGGYAPLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRANQTDQDSLPPYDVLDGI
LYRYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPITNGYRGQ
>Mature_546_residues
TAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLLRPGFLAHCEQALARIAAATRG
IVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNYAVFDERRYFDVDPDGESCVVTVKGVQVGVVICEDLWFAEPL
ANTVRAGAELVLVPNASPYERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQW
LVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDSALVLAMAVDALGGDNVTAVRL
PSRYTATLSNDLADEQCRALGVKLETIAIEPAFEGLLGALGPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTTG
NKSEYAVGYATIYGDMCGGYAPLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRANQTDQDSLPPYDVLDGIL
YRYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPITNGYRGQ

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59294; Mature: 59162

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLL
CCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCHHHEE
RPGFLAHCEQALARIAAATRGIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNY
CCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCC
AVFDERRYFDVDPDGESCVVTVKGVQVGVVICEDLWFAEPLANTVRAGAELVLVPNASPY
EEECCCEEECCCCCCCEEEEEEECEEEEEEEECHHHHHHHHHHHHHCCCEEEEECCCCHH
ERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQ
HHCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCHHHHHHHHH
WLVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDS
EEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHH
ALVLAMAVDALGGDNVTAVRLPSRYTATLSNDLADEQCRALGVKLETIAIEPAFEGLLGA
HHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHH
LGPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTTGNKSEYAVGYATIYGDMCGG
HCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEHHHCCC
YAPLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRANQTDQDSLPPYDVLDGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
LYRYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPI
HHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCC
TNGYRGQ
CCCCCCC
>Mature Secondary Structure 
TAHTLRIAMAQFDFPVGGVTQNTDRIIEYIAAARDEFEADIVLFPELAISGYPPEDLLL
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCHHHEE
RPGFLAHCEQALARIAAATRGIVAVVGWPQSAGSVVYNAASVLRDGRIEATYRKRELPNY
CCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCC
AVFDERRYFDVDPDGESCVVTVKGVQVGVVICEDLWFAEPLANTVRAGAELVLVPNASPY
EEECCCEEECCCCCCCEEEEEEECEEEEEEEECHHHHHHHHHHHHHCCCEEEEECCCCHH
ERGKHAQRDALLAERTRESGAAIAYLNVVGGQDALVFDGASVVADGDGTVHPAAAAFVDQ
HHCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCEEEECCCCCCCHHHHHHHHH
WLVVDYAAGERSFTPVVWVDDGDESMDALAWRAVVRGLQDYCRKNGFSKVWLGLSGGIDS
EEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHH
ALVLAMAVDALGGDNVTAVRLPSRYTATLSNDLADEQCRALGVKLETIAIEPAFEGLLGA
HHHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHH
LGPMFEGTQPDITEENLQSRSRGVILMALSNKFGGLVLTTGNKSEYAVGYATIYGDMCGG
HCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEHHHCCC
YAPLKDLYKTEVFGLAKWRNTVGGAPVIPPAVISRPPSAELRANQTDQDSLPPYDVLDGI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
LYRYVDQEQSRDDIVAAGYAADTVEHVLRLVRLNEWKRHQSAPGPKVSRRAFGRERRYPI
HHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCC
TNGYRGQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]