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The map label for this gene is cobS [H]

Identifier: 78048869

GI number: 78048869

Start: 3791438

End: 3792172

Strand: Reverse

Name: cobS [H]

Synonym: XCV3313

Alternate gene names: 78048869

Gene position: 3792172-3791438 (Counterclockwise)

Preceding gene: 78048870

Following gene: 78048868

Centisome position: 73.23

GC content: 68.44

Gene sequence:

>735_bases
ATGATCGACGGCTTGCTTGCTGCGCTCGGGTTCCTGACCCGCATGCCGGTGCCCGCCCGGGTGTTCGCCCGGCCCGGCGC
GCAGGCGCGTTCGCTGCCGTGGTACCCGGCCGTCGGACTGCTGATCGGCGCAGTGGTGAGCGCGTTGGCGTGGTGTTTGC
GGGATGCGGCGGCGTTGCTCGGGGCCGCGATGTTGTTGAGCAGCTGGGTCTGGTTTACCGGCGCCTTGCACATCGACGGC
CTGGCAGACACCGCCGATGCCTGGGTCGGCGGCATGGGCGATCGCACGCGCACCCTGGCGATCATGAAAGATCCCACCAG
CGGCCCGATGGCGGTGACTGCGATCGTGCTGATCTTGCTGCTCAAATACACGGCGCTGGCTGCCCTGTTGCCCACGGCGC
CGGGTGGGGTGTGGCTTGCTCCGCTGCTGGCGCGCACGGCGCTGGTAGCGGCTTTTTTGACGACGCCGTACGTGCGTGCC
GGCGGGCTTGGCAGTGGCCTGGTTGGCAGTTCGCGTGCTGGCTTGCTGTTGTCGTTGCTGCTCGGGCTGGCCTGCGCCTT
CGCCGTCGGGCGCTTGCGCGGCATGCTGGCCTGCGCAGTGGCGAGTGTGGTGTTGGTAATGTGGCGTCGTGCCTGCCTGC
AGCGCCTGGGCGGCATGACCGGCGATACCTGCGGTGCATTGGTGGAGATCAGCGAAACAGCGGTGCTGGTGGCCTTGGCG
CTGCAAACGAGCTGA

Upstream 100 bases:

>100_bases
ACATGACCGACATCGCCGTGCCGCACGCCAGCCTGCATCCGCTGGCGTGGCCACCGGCCGTCGCGACGCCGATAGCCGTG
TCCATGGCGTCCAGCTCCGG

Downstream 100 bases:

>100_bases
AGGCTGGGTGCGACGCGATTGCGCTGAAAACGGGCAAGCAGGCCCGCTGCGTGGAGTGTTTGGATCGCTGCTTTGTTTCA
ACGCCGCAGCTGCCCGACGA

Product: cobalamin synthase

Products: Cobalamin 5'-Phosphate; GMP; Cobalamin [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIDGLLAALGFLTRMPVPARVFARPGAQARSLPWYPAVGLLIGAVVSALAWCLRDAAALLGAAMLLSSWVWFTGALHIDG
LADTADAWVGGMGDRTRTLAIMKDPTSGPMAVTAIVLILLLKYTALAALLPTAPGGVWLAPLLARTALVAAFLTTPYVRA
GGLGSGLVGSSRAGLLLSLLLGLACAFAVGRLRGMLACAVASVVLVMWRRACLQRLGGMTGDTCGALVEISETAVLVALA
LQTS

Sequences:

>Translated_244_residues
MIDGLLAALGFLTRMPVPARVFARPGAQARSLPWYPAVGLLIGAVVSALAWCLRDAAALLGAAMLLSSWVWFTGALHIDG
LADTADAWVGGMGDRTRTLAIMKDPTSGPMAVTAIVLILLLKYTALAALLPTAPGGVWLAPLLARTALVAAFLTTPYVRA
GGLGSGLVGSSRAGLLLSLLLGLACAFAVGRLRGMLACAVASVVLVMWRRACLQRLGGMTGDTCGALVEISETAVLVALA
LQTS
>Mature_244_residues
MIDGLLAALGFLTRMPVPARVFARPGAQARSLPWYPAVGLLIGAVVSALAWCLRDAAALLGAAMLLSSWVWFTGALHIDG
LADTADAWVGGMGDRTRTLAIMKDPTSGPMAVTAIVLILLLKYTALAALLPTAPGGVWLAPLLARTALVAAFLTTPYVRA
GGLGSGLVGSSRAGLLLSLLLGLACAFAVGRLRGMLACAVASVVLVMWRRACLQRLGGMTGDTCGALVEISETAVLVALA
LQTS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=251, Percent_Identity=36.6533864541833, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 25021; Mature: 25021

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDGLLAALGFLTRMPVPARVFARPGAQARSLPWYPAVGLLIGAVVSALAWCLRDAAALL
CCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAMLLSSWVWFTGALHIDGLADTADAWVGGMGDRTRTLAIMKDPTSGPMAVTAIVLILL
HHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
LKYTALAALLPTAPGGVWLAPLLARTALVAAFLTTPYVRAGGLGSGLVGSSRAGLLLSLL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHH
LGLACAFAVGRLRGMLACAVASVVLVMWRRACLQRLGGMTGDTCGALVEISETAVLVALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LQTS
HHCC
>Mature Secondary Structure
MIDGLLAALGFLTRMPVPARVFARPGAQARSLPWYPAVGLLIGAVVSALAWCLRDAAALL
CCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAAMLLSSWVWFTGALHIDGLADTADAWVGGMGDRTRTLAIMKDPTSGPMAVTAIVLILL
HHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
LKYTALAALLPTAPGGVWLAPLLARTALVAAFLTTPYVRAGGLGSGLVGSSRAGLLLSLL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHH
LGLACAFAVGRLRGMLACAVASVVLVMWRRACLQRLGGMTGDTCGALVEISETAVLVALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LQTS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-Cobinamide; Alpha-Ribazole 5'-Phosphate; Alpha-Ribazole [C]

Specific reaction: GDP-Cobinamide + Alpha-Ribazole 5'-Phosphate (or Alpha-Ribazole) = Cobalamin 5'-Phosphate (or GDP-Cobalamin) + GMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA