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The map label for this gene is aspS [H]

Identifier: 78048842

GI number: 78048842

Start: 3751279

End: 3753045

Strand: Reverse

Name: aspS [H]

Synonym: XCV3286

Alternate gene names: 78048842

Gene position: 3753045-3751279 (Counterclockwise)

Preceding gene: 78048844

Following gene: 78048840

Centisome position: 72.47

GC content: 66.04

Gene sequence:

>1767_bases
ATGCGTACCCACTTCTGCGGCCTGGTCGACGAGACCTTGATCGGCCAAACCGTCACTCTCGCCGGCTGGACCGACGTGGC
CCGCAACCTGGGCGGGGTGTGCTTCATCGACCTGCGCGACCACGAAGGCATCGTGCAGGTGACGGTGGAGCCGGTGGCCG
GCGACGACGCCAGCGCAGAGCTGTTCAAGGTGGCCGCCAGCCTGGGCTACGAGGACGTGTTGCAGGTGGAAGGTGTGGTG
CGCGCGCGTCATGCGGTCAACGACAAGCTGCGCAGCGGCAAGGTGGAAGTGATCGCCACGCGCATCAGCATCCTCAACAA
GGCCGCGCCACTGCCGTTCCACGCGCACGAAAACCCGGGCGAAGAAACCCGCCTGAAGTACCGCTACCTGGACCTGCGCC
GGCCGGAAATGCAGCGCATGCAGCGCACCCGCATCAAGCTGGTGCAGGCGCTGCGCCGGCATCTGGATGCGCGCGATTTC
CAGGACATCGAAACCCCGATCCTGACCAAGGCCACCCCGGAAGGCGCGCGCGACTTCCTGGTGCCTGCGCGCATGCATCC
GGGCGAGTTCTACGCCTTGCCGCAGAGCCCGCAGCTGTTCAAGCAGATCCTGATGGTGGCCGGCTTCGACCGTTACTACC
AGATCGCGCGCTGCTTCCGCGACGAAGCGCTGCGTGCCGACCGCCAGCTGGAATTCACCCAGCTCGACATGGAATTCGCC
TTCGTGCGCGAGCGCGATGTGCAGGATTTCGTCGAAGACATGATCCGCGCCATTTTCAAGGAAGTGGTGGATGTCGACCT
GGCGGCGCAGTTCCCGCGCATGACCTGGGCCGAGGCGATGCGTCGCTATGGCTCGGACAAGCCGGACCTGCGTATCGCGC
TGGAACTGGTCGACGTGGCCGAGCTGGTCAAGGCCAGCCAATTCCCGGTGTTCACCGCTGCCGCCGACGATGCCGATGGC
CGCGTTGCCGCGCTGCTCATTCCCGGTGGCGCCAGCCTGTCGCGCAAGCAGATCGACGAATACGCAGCGCATGCCGCCAA
ATACGGCGCCAAGGGCCTGGCCTACATCAAGCTGTCGGAGACCGGCGAGGTCAGTTCGCCGATCGCCAAGTTCTTCAGCG
AAGAATCCTTCGCCGCGCTGCTCAAGCATGTCGGCGCCAGCAATGGCGACATCGTGTTCTTCGGTGCCGGCGGCTACACC
AAGGTGTCCGACTTCATGGGCGCGCTGCGCCTGAAGGCCGGCAAGGACTTCAACCTGGTCGCCGACGGCTGGGCGCCGCT
GTGGGTCACCGACTTCCCGATGTTCGAATGGGACGATGAGGCGCAGCGTTACGTCGCGTTGCATCACCCCTTCACCGCAC
CGGCGGTGGACGATATTGCCGACCTGCGCGCCAACGCCCGCACCGCGGTCTCGCGCGGTTACGACATGGTGCTCAACGGC
AACGAAATCGGCGGCGGCTCGATCCGTATCCACCGCCCGGACATGCAGAGCGCCGTGTTCGAACTGCTCGGCATCGGCGC
CGAAGAAGCGCGCGCCAAGTTCGGCTTCCTGCTGGACGCGTTGAACTACGGCGCGCCGCCGCACGGCGGCATCGCCTTCG
GTATCGACCGCATCGCCGCGCTGATGGCCGGTACCGAGTCCATCCGCGACGTGATCCCGTTCCCCAAGACCACCGGCGCG
CAGGATCTGATGACCGACGCCCCGTCGCCGATCGCTGCCGACCAGCTCGCCGAAGTGCACGTGCAGGTGCGCCCGAAGCA
GGTCTGA

Upstream 100 bases:

>100_bases
GCGCCCATGAGCGCCCGCGCATTTGCTCCTGCCGCTCGCCGGCCCCAGAATATCCGGCTTTCCGGCGCTGTCCTGCGTCG
GGTTTTCAGCGGAGCTTTCG

Downstream 100 bases:

>100_bases
CCCGTCATCGAACACACGGCCACGCCCATTGCGTGGCCGTGCTGTTCATGCGCAGCGGGCAGCTGGCGCAAGGCGTTGCT
GGCCGATCTGCGCTGCACGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAELFKVAASLGYEDVLQVEGVV
RARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPGEETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVAELVKASQFPVFTAAADDADG
RVAALLIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSETGEVSSPIAKFFSEESFAALLKHVGASNGDIVFFGAGGYT
KVSDFMGALRLKAGKDFNLVADGWAPLWVTDFPMFEWDDEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALMAGTESIRDVIPFPKTTGA
QDLMTDAPSPIAADQLAEVHVQVRPKQV

Sequences:

>Translated_588_residues
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAELFKVAASLGYEDVLQVEGVV
RARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPGEETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVAELVKASQFPVFTAAADDADG
RVAALLIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSETGEVSSPIAKFFSEESFAALLKHVGASNGDIVFFGAGGYT
KVSDFMGALRLKAGKDFNLVADGWAPLWVTDFPMFEWDDEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALMAGTESIRDVIPFPKTTGA
QDLMTDAPSPIAADQLAEVHVQVRPKQV
>Mature_588_residues
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAELFKVAASLGYEDVLQVEGVV
RARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPGEETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVAELVKASQFPVFTAAADDADG
RVAALLIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSETGEVSSPIAKFFSEESFAALLKHVGASNGDIVFFGAGGYT
KVSDFMGALRLKAGKDFNLVADGWAPLWVTDFPMFEWDDEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALMAGTESIRDVIPFPKTTGA
QDLMTDAPSPIAADQLAEVHVQVRPKQV

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=600, Percent_Identity=39.6666666666667, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI45439306, Length=255, Percent_Identity=31.3725490196078, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI5031815, Length=288, Percent_Identity=26.0416666666667, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI194272210, Length=288, Percent_Identity=26.0416666666667, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788173, Length=593, Percent_Identity=55.1433389544688, Blast_Score=643, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=327, Percent_Identity=27.5229357798165, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790571, Length=286, Percent_Identity=28.6713286713287, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=605, Percent_Identity=35.8677685950413, Blast_Score=340, Evalue=9e-94,
Organism=Caenorhabditis elegans, GI17551876, Length=266, Percent_Identity=26.6917293233083, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=264, Percent_Identity=24.6212121212121, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=264, Percent_Identity=24.6212121212121, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=640, Percent_Identity=32.03125, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6323011, Length=250, Percent_Identity=30.8, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=257, Percent_Identity=29.1828793774319, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=174, Percent_Identity=24.7126436781609, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584738, Length=601, Percent_Identity=37.4376039933444, Blast_Score=358, Evalue=7e-99,
Organism=Drosophila melanogaster, GI17136276, Length=265, Percent_Identity=27.9245283018868, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640849, Length=256, Percent_Identity=27.34375, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24640851, Length=256, Percent_Identity=27.34375, Blast_Score=80, Evalue=6e-15,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 64994; Mature: 64994

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAE
CCCCHHCHHHHHHCCCEEEECCHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCHHH
LFKVAASLGYEDVLQVEGVVRARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCCCC
EETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDFQDIETPILTKATPEGARDFL
HHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEECCCCCCCHHCC
VPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEHHCCHHHH
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHH
ELVKASQFPVFTAAADDADGRVAALLIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSE
HHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
TGEVSSPIAKFFSEESFAALLKHVGASNGDIVFFGAGGYTKVSDFMGALRLKAGKDFNLV
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEE
ADGWAPLWVTDFPMFEWDDEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
ECCCCCEEEECCCCCCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAA
CEECCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LMAGTESIRDVIPFPKTTGAQDLMTDAPSPIAADQLAEVHVQVRPKQV
HHHCHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHEEEEECCCCC
>Mature Secondary Structure
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPVAGDDASAE
CCCCHHCHHHHHHCCCEEEECCHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCCCHHH
LFKVAASLGYEDVLQVEGVVRARHAVNDKLRSGKVEVIATRISILNKAAPLPFHAHENPG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCCCC
EETRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDFQDIETPILTKATPEGARDFL
HHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEECCCCCCCHHCC
VPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
CCCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEHHCCHHHH
FVRERDVQDFVEDMIRAIFKEVVDVDLAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHH
ELVKASQFPVFTAAADDADGRVAALLIPGGASLSRKQIDEYAAHAAKYGAKGLAYIKLSE
HHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
TGEVSSPIAKFFSEESFAALLKHVGASNGDIVFFGAGGYTKVSDFMGALRLKAGKDFNLV
CCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEE
ADGWAPLWVTDFPMFEWDDEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
ECCCCCEEEECCCCCCCCCHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAA
CEECCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LMAGTESIRDVIPFPKTTGAQDLMTDAPSPIAADQLAEVHVQVRPKQV
HHHCHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]