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The map label for this gene is mexA [H]

Identifier: 78048560

GI number: 78048560

Start: 3417213

End: 3418433

Strand: Reverse

Name: mexA [H]

Synonym: XCV3004

Alternate gene names: 78048560

Gene position: 3418433-3417213 (Counterclockwise)

Preceding gene: 78048562

Following gene: 78048559

Centisome position: 66.01

GC content: 68.47

Gene sequence:

>1221_bases
GTGCGTGCTCATGCCTCGTTTCGCCTGTCGTTGCTTGCTGTTGCTGTTGCCGTTGCCGCCACCGCCCTGCTCAGTGCCTG
CGGCAGCCCACCGGGAGGTCCGCCGCCGCAGGAAGGCACTCCGGAAATGGGCGTGCTGACCGTCAAGCAGCAACCGGTGA
CGCTCACCACCGAATTACCGGGGCGCACTGTTCCGTACCTGATCTCCGAAATTCGTCCGCAGGTGGGCGGCATCATCCAG
TCGCGCCAGTTCACCGAAGGCGGCGACGTCAAGGCTGGCCAGACCCTGTACCAGATCGATCCGGCCCAGTACCGCGCCAG
CTATGCCAGCGCGCAGGCGTCCCTGGCCAAGGCCGAGGCGACCCTGCGCACCGCGCAGCTCAAGGCCGAGCGCTACAAGG
AACTGGCGCAGATCAAGGCGATCAGCCAGCAGGAAGGCGACGACACCGATGCCGCGCTAGGCCAGGCCAAGGCCGACGTG
GCAGCCGGCAAGGCGAGCGTGGAAACCGCGCGCATCAACCTGGCGTTTGCGCGGCTCGACGCACCCATCTCCGGACGCAT
CGGCCGCTCCAGCGTGACCCCGGGTGCCCTGGTGACCGCCAACCAGGCCACGGCGCTGACCACCATCCAGCAGCTGGACC
CGATCTACATCGACGTCACCCAGCCCAGCAGTGCGGTGTTGCGCCTGCGGCAGGCGATGGCGCGCGGCGATCTGGAACAA
GCCGGCGACGGTGCGGCCAAGGTGTCGCTGTTGCTGGAAGACGGCAGCACCTACCCGTTGCAGGGGCACCTGGCGTTCTC
CGACGTGACCGTCGACCAGAACACCGGCTCGATCACCCTGCGCGCGGTCTTCCCCAACCCGAACGCCGAGCTGTTGCCAG
GCATGTACGTGCGCGCGGTGCTGCAGGAAGGCGTCAAGGAGCAGGGCATGCTGGTGCCGCAACAGGCGGTCTCGCGCAAC
GGCGCGGGCAAACCCACGGCCTTTGTCGTCGCCGCCGACCACAAGCTGCAGTTGCGCGTGCTGGAAACCGATCGCGCGGT
CGGCGACCAGTGGTTGGTGCGCAGCGGCCTGAAGCCCGGCGAGCAGGTGGTCGTCGAAGGCGCATCGCGCGCACGCGATG
GCATCGTGGTCAAGACCGTGCCGTGGCAGCCCAAGGCCACACCCGCCGCCGGCAATGTTGCCGGTCCTGCCGCCCCGACC
GCACCGCGTGCGGCCAACTAA

Upstream 100 bases:

>100_bases
ACCGACTGGGCAGAAATGCTTGACGGCCACGCATGTGCTTATTAGGATACCGAACCGTACCGTTTAGATTCAATCATCAC
ACCTCCTGCGGAGACTGCCC

Downstream 100 bases:

>100_bases
GGCGGGATCACACGTCATGGCACGTTTCTTCATCGATCGTCCGATCTTCGCCTGGGTGTTGGCCATCATCGTGATGCTGG
CCGGCATCCTGTCCATCGCC

Product: membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MRAHASFRLSLLAVAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELPGRTVPYLISEIRPQVGGIIQ
SRQFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEATLRTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADV
AAGKASVETARINLAFARLDAPISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQ
AGDGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAVLQEGVKEQGMLVPQQAVSRN
GAGKPTAFVVAADHKLQLRVLETDRAVGDQWLVRSGLKPGEQVVVEGASRARDGIVVKTVPWQPKATPAAGNVAGPAAPT
APRAAN

Sequences:

>Translated_406_residues
MRAHASFRLSLLAVAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELPGRTVPYLISEIRPQVGGIIQ
SRQFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEATLRTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADV
AAGKASVETARINLAFARLDAPISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQ
AGDGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAVLQEGVKEQGMLVPQQAVSRN
GAGKPTAFVVAADHKLQLRVLETDRAVGDQWLVRSGLKPGEQVVVEGASRARDGIVVKTVPWQPKATPAAGNVAGPAAPT
APRAAN
>Mature_406_residues
MRAHASFRLSLLAVAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELPGRTVPYLISEIRPQVGGIIQ
SRQFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEATLRTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADV
AAGKASVETARINLAFARLDAPISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQ
AGDGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAVLQEGVKEQGMLVPQQAVSRN
GAGKPTAFVVAADHKLQLRVLETDRAVGDQWLVRSGLKPGEQVVVEGASRARDGIVVKTVPWQPKATPAAGNVAGPAAPT
APRAAN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=387, Percent_Identity=50.9043927648579, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1786668, Length=367, Percent_Identity=53.6784741144414, Blast_Score=354, Evalue=6e-99,
Organism=Escherichia coli, GI1789929, Length=359, Percent_Identity=48.1894150417827, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI87082036, Length=320, Percent_Identity=29.6875, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI87081796, Length=172, Percent_Identity=29.6511627906977, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42367; Mature: 42367

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAHASFRLSLLAVAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCC
GRTVPYLISEIRPQVGGIIQSRQFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEA
CCHHHHHHHHHCHHHCCHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
TLRTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADVAAGKASVETARINLAFARLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEC
APISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQ
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCHHH
AGDGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAV
CCCCCEEEEEEEECCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHH
LQEGVKEQGMLVPQQAVSRNGAGKPTAFVVAADHKLQLRVLETDRAVGDQWLVRSGLKPG
HHHHHHHCCCCCCHHHHHCCCCCCCEEEEEEECCEEEEEEEECCCCCCHHHHHHCCCCCC
EQVVVEGASRARDGIVVKTVPWQPKATPAAGNVAGPAAPTAPRAAN
HHEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAHASFRLSLLAVAVAVAATALLSACGSPPGGPPPQEGTPEMGVLTVKQQPVTLTTELP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCC
GRTVPYLISEIRPQVGGIIQSRQFTEGGDVKAGQTLYQIDPAQYRASYASAQASLAKAEA
CCHHHHHHHHHCHHHCCHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
TLRTAQLKAERYKELAQIKAISQQEGDDTDAALGQAKADVAAGKASVETARINLAFARLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEC
APISGRIGRSSVTPGALVTANQATALTTIQQLDPIYIDVTQPSSAVLRLRQAMARGDLEQ
CCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCHHH
AGDGAAKVSLLLEDGSTYPLQGHLAFSDVTVDQNTGSITLRAVFPNPNAELLPGMYVRAV
CCCCCEEEEEEEECCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHH
LQEGVKEQGMLVPQQAVSRNGAGKPTAFVVAADHKLQLRVLETDRAVGDQWLVRSGLKPG
HHHHHHHCCCCCCHHHHHCCCCCCCEEEEEEECCEEEEEEEECCCCCCHHHHHHCCCCCC
EQVVVEGASRARDGIVVKTVPWQPKATPAAGNVAGPAAPTAPRAAN
HHEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]