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The map label for this gene is pmbA [H]

Identifier: 78048476

GI number: 78048476

Start: 3321326

End: 3322693

Strand: Reverse

Name: pmbA [H]

Synonym: XCV2920

Alternate gene names: 78048476

Gene position: 3322693-3321326 (Counterclockwise)

Preceding gene: 78048478

Following gene: 78048475

Centisome position: 64.16

GC content: 68.86

Gene sequence:

>1368_bases
TTGAACGCACTCTCCTCCGAAATCCGCGTCACCGACGACAGCCTGCAGCGCCTGGATGCGCTCACCGACATCTCCCAGCG
CCTGCTCGAGCGCGCGCGCGCCGCCGGCGCCACGCAGGCCGAAGTCAGCTGCAGCGAAGAGCGCGGCCTGGACGTCAATG
TGCGGCTGGGCGATGTGGAGACGGTCGAATCCACGCGCGATCGTGGCATTGCGGTCACGGTCTACTTCGGCAAGCGCAAG
GGCAGTGCCAGCACTGCCGACCTGCAGGATTCCAGCCTGGAATCCACCGTGGCGCAGGCCTGCGCGATCGCCCGCTTCAC
CGAAGACGACGTGGCTGCCGGCCTGGCCGACCCGGAGTTGATGGCGCGCGAGTTCCCCGAGCTGGACAGCTGGCATCCGT
GGGCGCTGGACGCCGATACCGCCGTGGATCTGGCGCTGGCCTGCGAAACCGCCGGCCGCGACGCCGACGCGCGCATCAGC
AACTCCGACGGCGCCTCGGCCAGCACCGGGCTCAGCCTGTCGGTGTACGCCAATTCGCATGGCTTCATCGGCCGCGAGCG
CGGTACCCATCATTCGATCAGCTGCGCGCTGATCGCCGGGCAGGGCGACGGCATGCAGCGCGATGGCTGGTACAGCAGTG
CACTGGCACGCGAGGACCTGGAAGCGCCCGACGCCATCGGCCGCCATGCCGCCGCGCGTACCGTGGCGCGCTTGCAGCCG
CGTTCGCTGCCCACCGGTCAGTTGCCGGTGCTGTTCGCTCCGGAAGTGGCGCGCTCGCTGGTCGGCCACCTGCTCGGCGC
GGTGTCCGGCGGCGCGCTGTATCGCCGTGCCAGCTTCCTGCTCGACAGCGTCGGCAGCACGTTGTTCCCGGACTGGTTCA
ACATCGAAGAGCTGCCGCACCTGCGCCGCGGTTTGCGCTCGGCCGCCTTCGACGGCGAAGGCGTGGCGACGCGGCGTTCG
GCGCTGATCAGCGACGGTGTGCTGCAGCGCTATGTGCTGGGCAGCTATTCGGCGCGCAAGCTGGGCCTGCAGACCACCGC
CAACGCCGGTGGCGTCCACAACCTGCAGGTCACCGCCAATGCGGGCGATCTGGCGTCGATGATTGCCGGCATGTCGCGCG
GCCTGCTGGTCACCGAACTGATGGGCAACGGCGTCAATCCCGTCACCGGCAACTATTCGCGCGGGGCCGGCGGCTTCTGG
ATCGAAAACGGCGTGATTGCCTACCCGGTCGATGGCCTGACCATCGCCGGCAATCTGCGCGACATGTTCGCCGGCATCGA
AGCGGTGGGCAGCGATGTCGACCCGCGCTCGCACGTCAAGATCGGCTCGGTGCTGATCAACCGCATGACGGTGGCAGGCG
ACAGCTGA

Upstream 100 bases:

>100_bases
CGGCCGGGCGTCGGCGGAATAACTCCGCACCGAATTCAAACATTCCGGTCATGGATGGCCGGGCTTTCGCGGACGGATCC
GCGTTTCCAAGGATAAAGCA

Downstream 100 bases:

>100_bases
GCGGCACCGCTGTGTCGGCTTCGCCGGTTTCCGGGCGGCTTGTCGTGTAGGCGCACGCCTCACGAGCCGCCGCTCGCACC
TGACGATGCATCTGCGTAAC

Product: putative modulator of DNA gyrase; peptidase U62 protein PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MNALSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVETVESTRDRGIAVTVYFGKRK
GSASTADLQDSSLESTVAQACAIARFTEDDVAAGLADPELMAREFPELDSWHPWALDADTAVDLALACETAGRDADARIS
NSDGASASTGLSLSVYANSHGFIGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSTLFPDWFNIEELPHLRRGLRSAAFDGEGVATRRS
ALISDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTANAGDLASMIAGMSRGLLVTELMGNGVNPVTGNYSRGAGGFW
IENGVIAYPVDGLTIAGNLRDMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS

Sequences:

>Translated_455_residues
MNALSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVETVESTRDRGIAVTVYFGKRK
GSASTADLQDSSLESTVAQACAIARFTEDDVAAGLADPELMAREFPELDSWHPWALDADTAVDLALACETAGRDADARIS
NSDGASASTGLSLSVYANSHGFIGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSTLFPDWFNIEELPHLRRGLRSAAFDGEGVATRRS
ALISDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTANAGDLASMIAGMSRGLLVTELMGNGVNPVTGNYSRGAGGFW
IENGVIAYPVDGLTIAGNLRDMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS
>Mature_455_residues
MNALSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVETVESTRDRGIAVTVYFGKRK
GSASTADLQDSSLESTVAQACAIARFTEDDVAAGLADPELMAREFPELDSWHPWALDADTAVDLALACETAGRDADARIS
NSDGASASTGLSLSVYANSHGFIGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSTLFPDWFNIEELPHLRRGLRSAAFDGEGVATRRS
ALISDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTANAGDLASMIAGMSRGLLVTELMGNGVNPVTGNYSRGAGGFW
IENGVIAYPVDGLTIAGNLRDMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=414, Percent_Identity=47.8260869565217, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47925; Mature: 47925

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVE
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEECCCH
TVESTRDRGIAVTVYFGKRKGSASTADLQDSSLESTVAQACAIARFTEDDVAAGLADPEL
HHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHH
MAREFPELDSWHPWALDADTAVDLALACETAGRDADARISNSDGASASTGLSLSVYANSH
HHHHCCCCCCCCCCEECCCHHHHHEEEECCCCCCCCCEECCCCCCCCCCCEEEEEEECCC
GFIGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSTLFPDWFNIEELPH
CCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCCCCHHHHHH
LRRGLRSAAFDGEGVATRRSALISDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTAN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEECCCCCCEEEEEEECC
AGDLASMIAGMSRGLLVTELMGNGVNPVTGNYSRGAGGFWIENGVIAYPVDGLTIAGNLR
CHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEECCHH
DMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHEEECCCC
>Mature Secondary Structure
MNALSSEIRVTDDSLQRLDALTDISQRLLERARAAGATQAEVSCSEERGLDVNVRLGDVE
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEEEECCCH
TVESTRDRGIAVTVYFGKRKGSASTADLQDSSLESTVAQACAIARFTEDDVAAGLADPEL
HHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHH
MAREFPELDSWHPWALDADTAVDLALACETAGRDADARISNSDGASASTGLSLSVYANSH
HHHHCCCCCCCCCCEECCCHHHHHEEEECCCCCCCCCEECCCCCCCCCCCEEEEEEECCC
GFIGRERGTHHSISCALIAGQGDGMQRDGWYSSALAREDLEAPDAIGRHAAARTVARLQP
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
RSLPTGQLPVLFAPEVARSLVGHLLGAVSGGALYRRASFLLDSVGSTLFPDWFNIEELPH
CCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHCCCCCCHHHHHH
LRRGLRSAAFDGEGVATRRSALISDGVLQRYVLGSYSARKLGLQTTANAGGVHNLQVTAN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEECCCCCCEEEEEEECC
AGDLASMIAGMSRGLLVTELMGNGVNPVTGNYSRGAGGFWIENGVIAYPVDGLTIAGNLR
CHHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEECCHH
DMFAGIEAVGSDVDPRSHVKIGSVLINRMTVAGDS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]