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The map label for this gene is pinE [H]

Identifier: 78047883

GI number: 78047883

Start: 2658286

End: 2658900

Strand: Reverse

Name: pinE [H]

Synonym: XCV2327

Alternate gene names: 78047883

Gene position: 2658900-2658286 (Counterclockwise)

Preceding gene: 78047884

Following gene: 78047882

Centisome position: 51.35

GC content: 60.65

Gene sequence:

>615_bases
ATGCTGATCGGATATGCCCGCGTCTCCAAAGCCGATGGCTCGCAGTCTCTCGACCTGCAGCACGACGCCTTGCGCGCCGC
AGGTGTCGAACGGGACAATATCTATGATGATCTTGCTTCCGGCGGTCGTGATGATCGCCCTGGCTTGACTGCCTGCCTCA
AGTCATTGCGTGACGGCGATGTGCTGGTGGTCTGGAAGCTCGATCGCCTCGGACGATCGCTTGCCCATCTGGTCAACACG
GTGAAGGAGCTGTCAGACCGCAAGATCGGCCTGCGGGTTCTGACTGGAAAGGGCGCTCAGATCGACACCACGACTGCGTC
CGGTCGCATGGTGTTCGGAATCTTCGCCACCTTGGCCGAGTTCGAGCGGGATCTGATCCGAGAGCGCACCATGGCGGGTC
TCGCCTCCGCGAGAGCGCGCGGTCGCAAGGGCGGACGAAAATTCGCGCTCACCAAAGCTCAGGTGCGTCTCGCGCAAGCC
GCCATGGCCCAGCGCGATACTTCAGTTTCCGATCTCTGCAAGGAACTCGGCATCGAGCGCGTCACTCTCTACCGATATGT
CGGTCCCAAAGGCGAGCTCAGAGACCATGGAAAGCATGTTCTCGGACTTACGTAG

Upstream 100 bases:

>100_bases
ACCCTTTGCATGTCTTAGACAGAATGACGGGTTAAGTGACAAATTTTGACGACCAGAACTTTCCGGTGCACACTGTCACA
TAATCGAACGTTTGTGATAC

Downstream 100 bases:

>100_bases
CAACTCGTTTCTTTTCGCAGGTTGAGCCACCTCCGCGCTTCATCAGAAAACTGAAGGAACCTCCATTGAATCGAACTAAT
ATTTTTTTTGGTGAATCGCA

Product: putative resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRDDRPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNT
VKELSDRKIGLRVLTGKGAQIDTTTASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQA
AMAQRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGKHVLGLT

Sequences:

>Translated_204_residues
MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRDDRPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNT
VKELSDRKIGLRVLTGKGAQIDTTTASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQA
AMAQRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGKHVLGLT
>Mature_204_residues
MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRDDRPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNT
VKELSDRKIGLRVLTGKGAQIDTTTASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQA
AMAQRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGKHVLGLT

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=160, Percent_Identity=54.375, Blast_Score=146, Evalue=8e-37,
Organism=Escherichia coli, GI1787827, Length=188, Percent_Identity=32.9787234042553, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1787638, Length=188, Percent_Identity=32.4468085106383, Blast_Score=91, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22262; Mature: 22262

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRDDRPGLTACLKSLRDGD
CEECCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
VLVVWKLDRLGRSLAHLVNTVKELSDRKIGLRVLTGKGAQIDTTTASGRMVFGIFATLAE
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHH
FERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQAAMAQRDTSVSDLCKELGIER
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
VTLYRYVGPKGELRDHGKHVLGLT
EEEEEEECCCCCHHHCCCEECCCC
>Mature Secondary Structure
MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRDDRPGLTACLKSLRDGD
CEECCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCC
VLVVWKLDRLGRSLAHLVNTVKELSDRKIGLRVLTGKGAQIDTTTASGRMVFGIFATLAE
EEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHH
FERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQAAMAQRDTSVSDLCKELGIER
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
VTLYRYVGPKGELRDHGKHVLGLT
EEEEEEECCCCCHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]