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The map label for this gene is merA [H]

Identifier: 78047859

GI number: 78047859

Start: 2636276

End: 2637643

Strand: Reverse

Name: merA [H]

Synonym: XCV2303

Alternate gene names: 78047859

Gene position: 2637643-2636276 (Counterclockwise)

Preceding gene: 78047860

Following gene: 78047858

Centisome position: 50.93

GC content: 64.11

Gene sequence:

>1368_bases
ATGCAGGCAAGGAAAGTAGATATCGCCATCATCGGTGCTGGAACCGCCGGCATCACGGCATTTCATGCACTCAAGGCGGC
AGGAAAGGATGTCGTCCTGATCGATCGTGGACCGCTGGGGACGACCTGCGCGCGCGTAGGCTGCATGCCGTCGAAGGCCG
CTTTGCATGCGGGGATGCGCTGGACGACGGCCAAGGAGTTGCCCGCGGGCGTCACGCTCTCCGCACAAGGGCCGCAGGCT
TTGTGGCGGCATACCCGCCAGACGCGGGATGCTCTGGCCGGTGCTGCCGCGGAGCGCACGCGCAAGGCCGTCGGCGAAAA
CCTGATCATGGCCGAGGCTCGCTTTGTCGCGCCAGGCACCTTGGAAGTCGGCGACCAGCGTATCGAAGCGAAGGCTTTCA
TCGTGGCCACGGGCTCGCATCCGGTGGTGCCCAAGTCGCTGGACGGCGCGGCCTCCCACATCCTCACCACCGACACCCTT
TTCGAGCAGAAAGAACTGCCGCAGCGCCTGGGGATTCTAGGACTGGGAGCGATCGGCCTGGAACTTGGCCTGGCCCTGTC
GCGGCTTGACGTGCAGGTGATCGGTGCCGACCAACAGGACACGATCGGCGGTATTCAAGATCCCGAGGTGCAGGATCGGG
CGCTTTCGAGGTTCGGCGGCGAGTTTCCCATGTGGCTGGGAGCACCCGTCGAAGTTACCCTGGCAGGCGACAAGGTGCGC
ATGCGCACCGACAAACATGAAGCGCTCGTGGATCGCTTGCTGGTCGTGACTGGCCGCCAGCCCAATACCGAAGCGCTCAA
TCTGCAAGCGGCAGGCATCCTATTGGACAAGGCAGGCCGGCCGAACATCGACGCCTCCACCATGCAGGCATCGGATGCAC
CGATCTTCTTCGCGGGCGATGTGCAGCCGGATCGTCCGCTGATGCACGAGGCCGCCGACGAAGGCCAGATGGCGGCCCAG
GCCGCCTTGACGCGCCTGCGTGGCGAACAATGGCAGGGGGCGCCGCGTCGGGTGCCGATCAGCATCCTGTTCACCGATCC
CGACGTCTGCGCTGTCGGCATGTCCTACCAGGCCGCAATGAAGAAAGATGCCGTGGTCGGCATCGCGGAAGGTAGTGGCA
ACGGGCGCTCGAAGATTCTCGGTGCCCAGGACAACCTGCTGCGCATCTACGTCGAACCCGGCACTGGCTTGTTGCTTGGG
GCCAGCATGTTGCTGACGCAAGGCGAGCATCTGGCGCACCTGCTCGCCTGGGCAATCCAGGCCAAGCAGACCGTCAATGA
CCTGCTGACGATGCCGTTCTACCACCCATCCATCGAGGAGATGCTGCAGAGCGCCTTGAAGTCGGCATCCCAGCAACTCA
ACCCGTAA

Upstream 100 bases:

>100_bases
GTGTTCTCGATCACCTGCGTGTCCGTGGCGGCTCTCATGCTCATGCGCAACGTGGGCTAGAGATTCGCTATTTGGTTAAT
TGATTGGACGGAGATTTCCA

Downstream 100 bases:

>100_bases
ACGGACTCCTTCTTTCCGCCATCACGCCCGAACCATCATGAGCCAAAACATCCAGAACCAAGCATTGAAAGTGATCCTGC
ATGCGCCAACTCCCGCAGCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MQARKVDIAIIGAGTAGITAFHALKAAGKDVVLIDRGPLGTTCARVGCMPSKAALHAGMRWTTAKELPAGVTLSAQGPQA
LWRHTRQTRDALAGAAAERTRKAVGENLIMAEARFVAPGTLEVGDQRIEAKAFIVATGSHPVVPKSLDGAASHILTTDTL
FEQKELPQRLGILGLGAIGLELGLALSRLDVQVIGADQQDTIGGIQDPEVQDRALSRFGGEFPMWLGAPVEVTLAGDKVR
MRTDKHEALVDRLLVVTGRQPNTEALNLQAAGILLDKAGRPNIDASTMQASDAPIFFAGDVQPDRPLMHEAADEGQMAAQ
AALTRLRGEQWQGAPRRVPISILFTDPDVCAVGMSYQAAMKKDAVVGIAEGSGNGRSKILGAQDNLLRIYVEPGTGLLLG
ASMLLTQGEHLAHLLAWAIQAKQTVNDLLTMPFYHPSIEEMLQSALKSASQQLNP

Sequences:

>Translated_455_residues
MQARKVDIAIIGAGTAGITAFHALKAAGKDVVLIDRGPLGTTCARVGCMPSKAALHAGMRWTTAKELPAGVTLSAQGPQA
LWRHTRQTRDALAGAAAERTRKAVGENLIMAEARFVAPGTLEVGDQRIEAKAFIVATGSHPVVPKSLDGAASHILTTDTL
FEQKELPQRLGILGLGAIGLELGLALSRLDVQVIGADQQDTIGGIQDPEVQDRALSRFGGEFPMWLGAPVEVTLAGDKVR
MRTDKHEALVDRLLVVTGRQPNTEALNLQAAGILLDKAGRPNIDASTMQASDAPIFFAGDVQPDRPLMHEAADEGQMAAQ
AALTRLRGEQWQGAPRRVPISILFTDPDVCAVGMSYQAAMKKDAVVGIAEGSGNGRSKILGAQDNLLRIYVEPGTGLLLG
ASMLLTQGEHLAHLLAWAIQAKQTVNDLLTMPFYHPSIEEMLQSALKSASQQLNP
>Mature_455_residues
MQARKVDIAIIGAGTAGITAFHALKAAGKDVVLIDRGPLGTTCARVGCMPSKAALHAGMRWTTAKELPAGVTLSAQGPQA
LWRHTRQTRDALAGAAAERTRKAVGENLIMAEARFVAPGTLEVGDQRIEAKAFIVATGSHPVVPKSLDGAASHILTTDTL
FEQKELPQRLGILGLGAIGLELGLALSRLDVQVIGADQQDTIGGIQDPEVQDRALSRFGGEFPMWLGAPVEVTLAGDKVR
MRTDKHEALVDRLLVVTGRQPNTEALNLQAAGILLDKAGRPNIDASTMQASDAPIFFAGDVQPDRPLMHEAADEGQMAAQ
AALTRLRGEQWQGAPRRVPISILFTDPDVCAVGMSYQAAMKKDAVVGIAEGSGNGRSKILGAQDNLLRIYVEPGTGLLLG
ASMLLTQGEHLAHLLAWAIQAKQTVNDLLTMPFYHPSIEEMLQSALKSASQQLNP

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=474, Percent_Identity=27.6371308016878, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI50301238, Length=364, Percent_Identity=26.0989010989011, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1786307, Length=460, Percent_Identity=26.304347826087, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI87082354, Length=478, Percent_Identity=26.3598326359833, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=24.5575221238938, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789915, Length=344, Percent_Identity=24.4186046511628, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=475, Percent_Identity=25.8947368421053, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71983429, Length=351, Percent_Identity=26.7806267806268, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983419, Length=351, Percent_Identity=26.7806267806268, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=27.2536687631027, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325240, Length=485, Percent_Identity=23.9175257731959, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6325166, Length=387, Percent_Identity=23.5142118863049, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=28.7846481876333, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 48252; Mature: 48252

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQARKVDIAIIGAGTAGITAFHALKAAGKDVVLIDRGPLGTTCARVGCMPSKAALHAGMR
CCCEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCHHHHHCCCC
WTTAKELPAGVTLSAQGPQALWRHTRQTRDALAGAAAERTRKAVGENLIMAEARFVAPGT
CCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
LEVGDQRIEAKAFIVATGSHPVVPKSLDGAASHILTTDTLFEQKELPQRLGILGLGAIGL
EECCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHCEEEEHHHHH
ELGLALSRLDVQVIGADQQDTIGGIQDPEVQDRALSRFGGEFPMWLGAPVEVTLAGDKVR
HHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCEEEEEECCEEE
MRTDKHEALVDRLLVVTGRQPNTEALNLQAAGILLDKAGRPNIDASTMQASDAPIFFAGD
ECCCHHHHHHHHHHEEECCCCCCCEEEEEEEEEEEECCCCCCCCHHHEECCCCCEEEECC
VQPDRPLMHEAADEGQMAAQAALTRLRGEQWQGAPRRVPISILFTDPDVCAVGMSYQAAM
CCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCEEEECCCHHHHH
KKDAVVGIAEGSGNGRSKILGAQDNLLRIYVEPGTGLLLGASMLLTQGEHLAHLLAWAIQ
CCCCEEEEECCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AKQTVNDLLTMPFYHPSIEEMLQSALKSASQQLNP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQARKVDIAIIGAGTAGITAFHALKAAGKDVVLIDRGPLGTTCARVGCMPSKAALHAGMR
CCCEEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCHHHHHCCCC
WTTAKELPAGVTLSAQGPQALWRHTRQTRDALAGAAAERTRKAVGENLIMAEARFVAPGT
CCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
LEVGDQRIEAKAFIVATGSHPVVPKSLDGAASHILTTDTLFEQKELPQRLGILGLGAIGL
EECCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHHHCEEEEHHHHH
ELGLALSRLDVQVIGADQQDTIGGIQDPEVQDRALSRFGGEFPMWLGAPVEVTLAGDKVR
HHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCEEEEEECCEEE
MRTDKHEALVDRLLVVTGRQPNTEALNLQAAGILLDKAGRPNIDASTMQASDAPIFFAGD
ECCCHHHHHHHHHHEEECCCCCCCEEEEEEEEEEEECCCCCCCCHHHEECCCCCEEEECC
VQPDRPLMHEAADEGQMAAQAALTRLRGEQWQGAPRRVPISILFTDPDVCAVGMSYQAAM
CCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCEEEECCCHHHHH
KKDAVVGIAEGSGNGRSKILGAQDNLLRIYVEPGTGLLLGASMLLTQGEHLAHLLAWAIQ
CCCCEEEEECCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHH
AKQTVNDLLTMPFYHPSIEEMLQSALKSASQQLNP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]