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The map label for this gene is nodW [H]

Identifier: 78047660

GI number: 78047660

Start: 2409842

End: 2410453

Strand: Reverse

Name: nodW [H]

Synonym: XCV2104

Alternate gene names: 78047660

Gene position: 2410453-2409842 (Counterclockwise)

Preceding gene: 78047661

Following gene: 78047658

Centisome position: 46.55

GC content: 64.54

Gene sequence:

>612_bases
ATGAGCGAGCCGGCCCCGGTGGTGTATCTCATCGACGACGATGCCTCGATGCGCGCTGCACTGGAGGATCTGTTCGCCTC
GGTCGGCTTGCAGGTCTACGCGTTCGGTTCCACCGATCAATTCCTCGCGCACCGGTTGCACGAGGCACCCGCCTGCCTGG
TGCTGGACATTCGCATGCCGGGCCAGAGCGGGATGGAGTTCCATCGGCGCATGGTCGAATCCGGCGTTGCCCTGCCCACC
ATCTTCATTACCGGCCATGGCGATATCGCGATGAGCGTGGAGGCGATGAAGAACGGCGCGATCGAATTCCTGACCAAACC
GTTCCGCGATCAGGCCCTGCTGGATGCCATTCAGGACGGCATCCGGCGTGACCGTGCGCGCCGCCAGAGCGAGGCGGTGG
CCGCGCAATTGCGTGCACGTTGGGAGTCGCTGAGTAGCGGCGAGCAGGATGTCACGCGATTGGTGGTGCAAGGTCTGTTG
AACAAGCAGATCGCCGCGCGCCTGCACCTGAGCGAGATCACCGTGAAAGTGCGGCGTGGCCAGGCCATGCGCAAGATGCA
GGCTGGCTCGCTGGCCGAACTGGTGCGCCTGGCCGAACGCATCGGGCTGTGA

Upstream 100 bases:

>100_bases
TCATGGAAGCCAACGGCGGGCAGATCTGGGCCGAGCCGGCCGGTGTACGCGGCGCGCGCTTCGGCCTGCGTCTGCCCCTG
GTCGCGACGGAGCGCAGGCC

Downstream 100 bases:

>100_bases
TGCGCGCTGTGTGACCGGTGCGCAGGCGTGCTGTGCTAGCTGCCCGCGCACGCGCCATCGGCGGGCATCCAGCCGCCATG
ACGGCCTGCGTGCTCCAGGA

Product: two-component system response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSEPAPVVYLIDDDASMRAALEDLFASVGLQVYAFGSTDQFLAHRLHEAPACLVLDIRMPGQSGMEFHRRMVESGVALPT
IFITGHGDIAMSVEAMKNGAIEFLTKPFRDQALLDAIQDGIRRDRARRQSEAVAAQLRARWESLSSGEQDVTRLVVQGLL
NKQIAARLHLSEITVKVRRGQAMRKMQAGSLAELVRLAERIGL

Sequences:

>Translated_203_residues
MSEPAPVVYLIDDDASMRAALEDLFASVGLQVYAFGSTDQFLAHRLHEAPACLVLDIRMPGQSGMEFHRRMVESGVALPT
IFITGHGDIAMSVEAMKNGAIEFLTKPFRDQALLDAIQDGIRRDRARRQSEAVAAQLRARWESLSSGEQDVTRLVVQGLL
NKQIAARLHLSEITVKVRRGQAMRKMQAGSLAELVRLAERIGL
>Mature_202_residues
SEPAPVVYLIDDDASMRAALEDLFASVGLQVYAFGSTDQFLAHRLHEAPACLVLDIRMPGQSGMEFHRRMVESGVALPTI
FITGHGDIAMSVEAMKNGAIEFLTKPFRDQALLDAIQDGIRRDRARRQSEAVAAQLRARWESLSSGEQDVTRLVVQGLLN
KQIAARLHLSEITVKVRRGQAMRKMQAGSLAELVRLAERIGL

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=102, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789809, Length=133, Percent_Identity=30.0751879699248, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=29.6875, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22423; Mature: 22292

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPAPVVYLIDDDASMRAALEDLFASVGLQVYAFGSTDQFLAHRLHEAPACLVLDIRMP
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECC
GQSGMEFHRRMVESGVALPTIFITGHGDIAMSVEAMKNGAIEFLTKPFRDQALLDAIQDG
CCCCHHHHHHHHHCCCCEEEEEEECCCCCEEHHHHHHCCHHHHHHCCCHHHHHHHHHHHH
IRRDRARRQSEAVAAQLRARWESLSSGEQDVTRLVVQGLLNKQIAARLHLSEITVKVRRG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAMRKMQAGSLAELVRLAERIGL
HHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SEPAPVVYLIDDDASMRAALEDLFASVGLQVYAFGSTDQFLAHRLHEAPACLVLDIRMP
CCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCEEEEEEECC
GQSGMEFHRRMVESGVALPTIFITGHGDIAMSVEAMKNGAIEFLTKPFRDQALLDAIQDG
CCCCHHHHHHHHHCCCCEEEEEEECCCCCEEHHHHHHCCHHHHHHCCCHHHHHHHHHHHH
IRRDRARRQSEAVAAQLRARWESLSSGEQDVTRLVVQGLLNKQIAARLHLSEITVKVRRG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAMRKMQAGSLAELVRLAERIGL
HHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]