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The map label for this gene is rpoN1 [H]

Identifier: 78047572

GI number: 78047572

Start: 2297360

End: 2298763

Strand: Reverse

Name: rpoN1 [H]

Synonym: XCV2016

Alternate gene names: 78047572

Gene position: 2298763-2297360 (Counterclockwise)

Preceding gene: 78047573

Following gene: 78047571

Centisome position: 44.39

GC content: 67.02

Gene sequence:

>1404_bases
ATGAAGACGACCATTTCCGCTCAGCTTGGCCAGCAACTGCACCTCACCCCGCAGTTGCTGCAGTCGATCCGTCTGTTGCA
ATTGGACGGCATGCAATTGGAACTGGAAATCCGTCGTGCCCTGGAAACCAATCCGTTGCTGGAGTTGGAAGAGCTGGCCG
ATAACGGGGATCAGGCCGCCACCGACAACGATGGCGCCAGCGATATCGCCGCGTTCGACGAACTGCCGGAAGGCACGATG
TGGGACGTGCAGGGCGGCAGTTGGAACGATGGGGACGACGATCGCATGCAGCGCATGGCCGCCGGCGAGTCGACCGACCC
GCATGTGCGCATCCTGCGCGAACTGGCGCTGGACCTGGACGAGGCGGCATTGGGGGCCGCTGCGTTCTGGCTGGAGCAGA
CCGACGACGCCGGTTATCTGACCGAAGCGCTGTCGACGCTGCAACAGCTGGGCGCCACCCGTCTGGGCATGACCGTGCAG
GCGGTGGAAGCGATCCGGCAGCGGTTGCTGCACGGCGACCCGGCCGGCCTGGCCGCCTGCGACCTGCGCGAGTGTCTGCA
GGCCCAGTTGAGCGCCCTGCCCGGCCGCGTCCCGGCACGCCACCTGGCTGCGCGCATCCTCGACGGCGATCTGGAGCTGC
TGGCCGCGCACGACTACACGGTGCTGGCATGCGCACACGATGCCGAAGTGGACGATGCCCGCGAAGCGGTGCGGCTGATC
CTGTCGCTGCAACCTCGCCCCGGCGACGACCTGCTGCAGGAAAGCAATGCCAGCGTGATCCCGGATGTGCTCGCCTGGCA
TGCCGACGACAGCTGGCGCGTCGCACTCAACCCGGCCACCACCCATCGCGTCAGCATCAATCCGGTGCACGAACGCGCGC
TGGCCGACGCCGGCGAGGCCGCGCAGCCGTTGCGCGACATGCTGCAGGAAGCGCGCTGGCTGACCCGCGGGCTGTCGATG
CGCTATGAGACCCTGCTGCGCGCCACGCGTGCCATCGTCGAACGCCAGGCCGCCTTCCTGGCGCGTGGCGAAGAAGCGAT
GGCACCGCTGACGCTGAAGGAAATCGCCGACGAGATCGGCATGCACGAATCGACCATTTCGCGCATCACCACCGGCAAAT
ACATCCAGACTCCACGCGGCACCTTCGAGCTCAAGCACTTCTTCGCCGTGCGGCTGGAAGGCGCCAGCGTCTCCGGCCAG
GCCGTCAAGGCGATGGTGCGCCGCTTGATCGAAAGCGAGCCGGCCGGCCGGCCGCTGGCCGACGAGGCGATTGCCGGATT
GCTGTCGCGGCAAGGCGTTAACATTGCGCGCCGCACCGTGGCCAAGTACCGCGAACAACTTGATATCGCACCGGCACGCG
AGCGCCGCCGCACCACTAAACCGCTGCTTGCCCGGGCAGGATAA

Upstream 100 bases:

>100_bases
ATGAGTCACCTCACCTGACTCCCTGATTAGAAACCCGTCGCGTTGTACTGCAAGATGCATTCCATCGCTGCTGCGTGCAG
CGCCCATCGGAAGCGCTTCC

Downstream 100 bases:

>100_bases
GGACTACTATGAGCAAACTCACCGTGCTGCTGGTCGACGACCACGAGGGCTTCATCAACGCTGCGATGCGTCATTTTCGC
AAGGTCGATTGGCTCAATAT

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDQAATDNDGASDIAAFDELPEGTM
WDVQGGSWNDGDDDRMQRMAAGESTDPHVRILRELALDLDEAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTVQ
AVEAIRQRLLHGDPAGLAACDLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLACAHDAEVDDAREAVRLI
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEAAQPLRDMLQEARWLTRGLSM
RYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIGMHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQ
AVKAMVRRLIESEPAGRPLADEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG

Sequences:

>Translated_467_residues
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDQAATDNDGASDIAAFDELPEGTM
WDVQGGSWNDGDDDRMQRMAAGESTDPHVRILRELALDLDEAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTVQ
AVEAIRQRLLHGDPAGLAACDLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLACAHDAEVDDAREAVRLI
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEAAQPLRDMLQEARWLTRGLSM
RYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIGMHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQ
AVKAMVRRLIESEPAGRPLADEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG
>Mature_467_residues
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDQAATDNDGASDIAAFDELPEGTM
WDVQGGSWNDGDDDRMQRMAAGESTDPHVRILRELALDLDEAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTVQ
AVEAIRQRLLHGDPAGLAACDLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLACAHDAEVDDAREAVRLI
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEAAQPLRDMLQEARWLTRGLSM
RYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIGMHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQ
AVKAMVRRLIESEPAGRPLADEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=481, Percent_Identity=39.0852390852391, Blast_Score=294, Evalue=1e-80,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51190; Mature: 51190

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDQAA
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHCCCCCCC
TDNDGASDIAAFDELPEGTMWDVQGGSWNDGDDDRMQRMAAGESTDPHVRILRELALDLD
CCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCHH
EAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTVQAVEAIRQRLLHGDPAGLAAC
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHCCCCCCHHHH
DLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLACAHDAEVDDAREAVRLI
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHH
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEA
HHCCCCCCHHHHHCCCCCCCCHHHHCCCCCCCEEEECCCCCEEEECCHHHHHHHHCCCHH
AQPLRDMLQEARWLTRGLSMRYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHC
MHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQAVKAMVRRLIESEPAGRPLA
CCHHHHHHHCCCCEEECCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCH
DEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHCCC
>Mature Secondary Structure
MKTTISAQLGQQLHLTPQLLQSIRLLQLDGMQLELEIRRALETNPLLELEELADNGDQAA
CCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHCCCCCCC
TDNDGASDIAAFDELPEGTMWDVQGGSWNDGDDDRMQRMAAGESTDPHVRILRELALDLD
CCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCHH
EAALGAAAFWLEQTDDAGYLTEALSTLQQLGATRLGMTVQAVEAIRQRLLHGDPAGLAAC
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHCCCCCCHHHH
DLRECLQAQLSALPGRVPARHLAARILDGDLELLAAHDYTVLACAHDAEVDDAREAVRLI
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHH
LSLQPRPGDDLLQESNASVIPDVLAWHADDSWRVALNPATTHRVSINPVHERALADAGEA
HHCCCCCCHHHHHCCCCCCCCHHHHCCCCCCCEEEECCCCCEEEECCHHHHHHHHCCCHH
AQPLRDMLQEARWLTRGLSMRYETLLRATRAIVERQAAFLARGEEAMAPLTLKEIADEIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHC
MHESTISRITTGKYIQTPRGTFELKHFFAVRLEGASVSGQAVKAMVRRLIESEPAGRPLA
CCHHHHHHHCCCCEEECCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCH
DEAIAGLLSRQGVNIARRTVAKYREQLDIAPARERRRTTKPLLARAG
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113171; 10984043 [H]