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The map label for this gene is fliA [H]

Identifier: 78047533

GI number: 78047533

Start: 2251433

End: 2252200

Strand: Reverse

Name: fliA [H]

Synonym: XCV1977

Alternate gene names: 78047533

Gene position: 2252200-2251433 (Counterclockwise)

Preceding gene: 78047534

Following gene: 78047532

Centisome position: 43.49

GC content: 67.06

Gene sequence:

>768_bases
ATGAGCACCGCTACCGCAACCACGGCCAGCGCGCAGTACCGCGCCGTGCAGCGCAACAACGCCAACGACGTGGTGACCCA
GCACGCCGACCTGGTCCGCCGCATCGCCCACCATCTGGCGGCGCGCCTGCCGGCCAGCGTGGAAGTGGACGACCTGATCC
AGGCCGGCATGATCGGCCTGATCGAAGCCTCGCGCAGCTACGACTCCGAACAGGGCGCTTCGTTCGAAACCTATGCCTCG
ATCCGCATCCGCGGCTCGATGATCGACGAGATTCGCAGGGGCGACTGGGTGCCGCGTTCGGTGCACCGCCGCGCCCGCGA
CGCTGCCGCCGCCGTGCGCAAGATCGAACAGAACACCGGCCGCGCCGCCGCCGCCACCGAAGTGGCCGCCGCGATGGAAA
TGCCGCTGCCCGATTACCTGCGTCTGATGGAAGATGCCGCACGTGGCCAGGTGCTGAGCCTGGAATCGCGCATCGAAGAT
CACGGCGAGCTGGACACCACCGCCAAGGGTGGCCCCAACCCGCAGCAGATGATGGAGCGTGGCGAATTCGGCCGGGAGCT
GGGCAAGGCCATCGGCCAACTGCCCGAGCGCGAGCAGCTGGTGCTGTCGCTGTACTACGAACAGGAATTGAACCTGAAGG
AAATCGGCGCCGTGCTGGGCGTCAGCGAATCGCGCGTGTGCCAGATCCATGGCCAGGCGGTGGTGCGTTTGCGTGGCCGC
CTCAAAGTCTTCGAACTGGCCGATGCCGGCGTCGAAGTCGACGACTGA

Upstream 100 bases:

>100_bases
CCGAAATCGCACGCCGTACCTCGCGCTGGCAGGCCCCGACCGTGCCGCGCGGCAACGTCGAGTTCTTTGTCGAACGCATC
ATCCAACGGGGAGTGGCCGC

Downstream 100 bases:

>100_bases
ATTTTTCGCGTAATTTTTCGTGTTAGGAGCTTGATGTGAACAAGAACATGCGGATCCTGATCGTGGACGACTTCTCGACG
ATGCGACGTATCGTCAAGAA

Product: RNA polymerase sigma factor for flagellar operon FliA

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSTATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEASRSYDSEQGASFETYAS
IRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIED
HGELDTTAKGGPNPQQMMERGEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGR
LKVFELADAGVEVDD

Sequences:

>Translated_255_residues
MSTATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEASRSYDSEQGASFETYAS
IRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIED
HGELDTTAKGGPNPQQMMERGEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGR
LKVFELADAGVEVDD
>Mature_254_residues
STATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGLIEASRSYDSEQGASFETYASI
RIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTGRAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDH
GELDTTAKGGPNPQQMMERGEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGRL
KVFELADAGVEVDD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=50.6912442396313, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27989; Mature: 27858

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGL
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IEASRSYDSEQGASFETYASIRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTG
HHHHHCCCCCCCCCEEHEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDHGELDTTAKGGPNPQQMMER
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCCCCCCHHHHHHH
GEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECHHHHHHHHCC
LKVFELADAGVEVDD
EEEEEECCCCCCCCC
>Mature Secondary Structure 
STATATTASAQYRAVQRNNANDVVTQHADLVRRIAHHLAARLPASVEVDDLIQAGMIGL
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IEASRSYDSEQGASFETYASIRIRGSMIDEIRRGDWVPRSVHRRARDAAAAVRKIEQNTG
HHHHHCCCCCCCCCEEHEEEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RAAAATEVAAAMEMPLPDYLRLMEDAARGQVLSLESRIEDHGELDTTAKGGPNPQQMMER
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHCCCCCCCCCCCCCHHHHHHH
GEFGRELGKAIGQLPEREQLVLSLYYEQELNLKEIGAVLGVSESRVCQIHGQAVVRLRGR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECHHHHHHHHCC
LKVFELADAGVEVDD
EEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]