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The map label for this gene is paaF [C]

Identifier: 78047455

GI number: 78047455

Start: 2145856

End: 2146653

Strand: Reverse

Name: paaF [C]

Synonym: XCV1899

Alternate gene names: 78047455

Gene position: 2146653-2145856 (Counterclockwise)

Preceding gene: 78047469

Following gene: 78047454

Centisome position: 41.45

GC content: 68.05

Gene sequence:

>798_bases
ATGCAGGACACCGGCTTGATCGTCGAAGACCACGGAGCGGTTCGCACCTTGCGATTGCATCGGCCCGCAGTACATAACGC
CTTCGATGCGACGCTGATCGCGGCGTTGACCGATGCATTACGCGAGGCGGGCCGGGCGTCGCAGATCCGCGTCGTCGTGC
TGACCGGTGATGGCGCGGCCTTTTCGGCCGGCGCAGACCTGAACTGGATGCGCGGCATGGCCAGCGCCAGCGAAGCCGAG
AACGCCGCCGACGCACTGGCGCTGGCGCAGCTGATGCGCACCCTGGATGAACTGCCCAAGCCCACCATCGCGCGCGTCAA
CGGCGCTGCTTTCGGCGGCGGTGTCGGCCTGGTGGCGTGTTGCGATATCGCCATCGGTTGTACCGAGGCGCGCTTCGGCC
TGACCGAAAGCAAGCTCGGCCTGCTGCCGGCGGTCATTTCGCCCTATGTGGTTGCAGCGATCGGCGCGCGCCAGGCGCGG
CGCTGGTTCGCAACCGCCGAGATCTTCGATGCCGGCACCGCGCAGTTGCTGGGTCTGCTGCACCAGCTGGTGGCCGCCGA
CCAGCTGGATATCGCAGTGGAACGCCAGGTTCGCCTGTTGCTGGCAGCGGCGCCGCTGGCCACCAGCAGCGCCAAGGCGC
TGGTGCGCTCGGTGCTGCCAACTGCCGACCGCGATGCCATCGATGCCGCCAATGCGGCCTTGATTGCCCGTCTGCGCGTG
TCGCCCGAAGGCCAGGAAGGCCTGGGTGCGTTTCTGGACAAGCGCAGCGCCGCGTGGATGCCGGAGAGCATGGCGTGA

Upstream 100 bases:

>100_bases
TGTTAGGAATGGTTCTCAATTCTAGCATCGACGCACCGCGTCATGGCTCCGGCGACCCGGCCCGCTATCATGGTTCGCAT
CCCATTCATGGACGTACGCA

Downstream 100 bases:

>100_bases
GCGCGATCGACCATATCGGCCTGGCCTGCAGCGACCTGCAAACCAGCACCGCGTTCTACCGGGCCGCGCTGGCGCCCCTG
GGCATCACGCTGCTGGTGGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRVVVLTGDGAAFSAGADLNWMRGMASASEAE
NAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVACCDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQAR
RWFATAEIFDAGTAQLLGLLHQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPTADRDAIDAANAALIARLRV
SPEGQEGLGAFLDKRSAAWMPESMA

Sequences:

>Translated_265_residues
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRVVVLTGDGAAFSAGADLNWMRGMASASEAE
NAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVACCDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQAR
RWFATAEIFDAGTAQLLGLLHQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPTADRDAIDAANAALIARLRV
SPEGQEGLGAFLDKRSAAWMPESMA
>Mature_265_residues
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRVVVLTGDGAAFSAGADLNWMRGMASASEAE
NAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVACCDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQAR
RWFATAEIFDAGTAQLLGLLHQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPTADRDAIDAANAALIARLRV
SPEGQEGLGAFLDKRSAAWMPESMA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=26.2948207171315, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI194097323, Length=272, Percent_Identity=27.2058823529412, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=25.78125, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45643119, Length=256, Percent_Identity=25.78125, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260275230, Length=256, Percent_Identity=25.78125, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI68989263, Length=184, Percent_Identity=30.9782608695652, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI213417737, Length=192, Percent_Identity=23.9583333333333, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI157694516, Length=192, Percent_Identity=23.9583333333333, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI70995211, Length=150, Percent_Identity=30.6666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=33.596837944664, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=30.5555555555556, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=27.5092936802974, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082183, Length=246, Percent_Identity=26.0162601626016, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=31.2252964426877, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790281, Length=170, Percent_Identity=31.7647058823529, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=206, Percent_Identity=30.0970873786408, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=28.2945736434109, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=259, Percent_Identity=27.7992277992278, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=234, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=25.390625, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=28.3582089552239, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=25.390625, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129971, Length=253, Percent_Identity=29.2490118577075, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653477, Length=253, Percent_Identity=29.2490118577075, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=25.5506607929515, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27568; Mature: 27568

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRVVVLTGDGAA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
FSAGADLNWMRGMASASEAENAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVAC
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCCCCHHHHHH
CDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQARRWFATAEIFDAGTAQLLGLL
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPTADRDAIDAANAALIARLRV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEC
SPEGQEGLGAFLDKRSAAWMPESMA
CCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQDTGLIVEDHGAVRTLRLHRPAVHNAFDATLIAALTDALREAGRASQIRVVVLTGDGAA
CCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCE
FSAGADLNWMRGMASASEAENAADALALAQLMRTLDELPKPTIARVNGAAFGGGVGLVAC
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCCCCHHHHHH
CDIAIGCTEARFGLTESKLGLLPAVISPYVVAAIGARQARRWFATAEIFDAGTAQLLGLL
HHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HQLVAADQLDIAVERQVRLLLAAAPLATSSAKALVRSVLPTADRDAIDAANAALIARLRV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEC
SPEGQEGLGAFLDKRSAAWMPESMA
CCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA