Click here to switch to the map view.

The map label for this gene is rpoE4 [H]

Identifier: 78047274

GI number: 78047274

Start: 1937085

End: 1937678

Strand: Reverse

Name: rpoE4 [H]

Synonym: XCV1718

Alternate gene names: 78047274

Gene position: 1937678-1937085 (Counterclockwise)

Preceding gene: 78047275

Following gene: 78047273

Centisome position: 37.42

GC content: 65.82

Gene sequence:

>594_bases
ATGCCTGCACCGATGAATGACACCGACACCCTGGGCGATGCCACCGACCGCATGCTGTTGCGCCGCATGGCCGGGGGCGA
TCGCGACGCGCTGGCCAGCATGTACCACAGCTATCACGGACGGCTGTGCCGCTTCCTGTCGCGGCTGACCCGCCGCCCGG
ACATCATCGAAGAGGCCATCAACGATTGCTTCTGGATCGCCTGGCAAAAGGCCGGCGACTTCCGCGGCGACTCGCAGGTC
TCCACCTGGATCATGGGCATCGCCTACCGCTGCGGACTCAAATGCCTGCGCCAGCATGACGGCGAAGCCATCGACGACAC
CGCCCTGGTGGAAGACCACGCCCCCAGCGCCGACGACAACGACGACCGCGAACTGCGCGACTGGCTGGACAAGGGCATGG
AGCGGTTGTCGCCGGACCAGCGCCTGGTGGTCGAACTGGTCTACGGCGTCGGCCACAAGCTCGAGGAAGTGGCCGACATC
ATGCAGTGCCCGGTTGGCACCATCAAAGCCCGGCTGTTCCACGCCCGGGTCAAATTACGCAATGTGCTGCCCGGCCTGGC
CGGCGGCGCCCCTTACACGGAGACCATGCAATGA

Upstream 100 bases:

>100_bases
CGGCCCCGGCGACATGCCGTGGCTGGGCACGGTCGCCAGAACCTTCTGATCCACACGCAACCTTTTGCCTGCCTGCGCCC
ACTAACGTGGGTTACCCGCC

Downstream 100 bases:

>100_bases
AGACGTTTTTCATCGCGCCTGGCAAGGAATGCTCGCACGCCTGGGAATTGATGCCGGCGGTGCTGCTGAACAGTGCCACC
GAGGAAGAAAACACCTGGCT

Product: RNA polymerase ECF-type sigma factor

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MPAPMNDTDTLGDATDRMLLRRMAGGDRDALASMYHSYHGRLCRFLSRLTRRPDIIEEAINDCFWIAWQKAGDFRGDSQV
STWIMGIAYRCGLKCLRQHDGEAIDDTALVEDHAPSADDNDDRELRDWLDKGMERLSPDQRLVVELVYGVGHKLEEVADI
MQCPVGTIKARLFHARVKLRNVLPGLAGGAPYTETMQ

Sequences:

>Translated_197_residues
MPAPMNDTDTLGDATDRMLLRRMAGGDRDALASMYHSYHGRLCRFLSRLTRRPDIIEEAINDCFWIAWQKAGDFRGDSQV
STWIMGIAYRCGLKCLRQHDGEAIDDTALVEDHAPSADDNDDRELRDWLDKGMERLSPDQRLVVELVYGVGHKLEEVADI
MQCPVGTIKARLFHARVKLRNVLPGLAGGAPYTETMQ
>Mature_196_residues
PAPMNDTDTLGDATDRMLLRRMAGGDRDALASMYHSYHGRLCRFLSRLTRRPDIIEEAINDCFWIAWQKAGDFRGDSQVS
TWIMGIAYRCGLKCLRQHDGEAIDDTALVEDHAPSADDNDDRELRDWLDKGMERLSPDQRLVVELVYGVGHKLEEVADIM
QCPVGTIKARLFHARVKLRNVLPGLAGGAPYTETMQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=182, Percent_Identity=30.2197802197802, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22234; Mature: 22102

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPMNDTDTLGDATDRMLLRRMAGGDRDALASMYHSYHGRLCRFLSRLTRRPDIIEEAI
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NDCFWIAWQKAGDFRGDSQVSTWIMGIAYRCGLKCLRQHDGEAIDDTALVEDHAPSADDN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
DDRELRDWLDKGMERLSPDQRLVVELVYGVGHKLEEVADIMQCPVGTIKARLFHARVKLR
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
NVLPGLAGGAPYTETMQ
HHCCCCCCCCCCHHCCC
>Mature Secondary Structure 
PAPMNDTDTLGDATDRMLLRRMAGGDRDALASMYHSYHGRLCRFLSRLTRRPDIIEEAI
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NDCFWIAWQKAGDFRGDSQVSTWIMGIAYRCGLKCLRQHDGEAIDDTALVEDHAPSADDN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
DDRELRDWLDKGMERLSPDQRLVVELVYGVGHKLEEVADIMQCPVGTIKARLFHARVKLR
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
NVLPGLAGGAPYTETMQ
HHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]