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The map label for this gene is nemA [H]

Identifier: 78046973

GI number: 78046973

Start: 1596073

End: 1597191

Strand: Reverse

Name: nemA [H]

Synonym: XCV1417

Alternate gene names: 78046973

Gene position: 1597191-1596073 (Counterclockwise)

Preceding gene: 78046981

Following gene: 78046970

Centisome position: 30.84

GC content: 66.58

Gene sequence:

>1119_bases
GTGATCAATTTCGACTGGTTGCTGATGTCCAAATCCACTCAATCCCCCTTGTTCTCGCCGGTGCGCCTGGGCGCGCTGGA
GCTTGCCAACCGCGTGATCATGGCCCCGCTGACGCGCAATCGCGCGGTCGCCGGCCAGGTGCCCTCGCCGCTTGCCGCTG
AGTATTACGGCCAGCGCGCCACCGCCGGCCTGATCGTGGCCGAAGGCACCCAGATCAGTCCGCTGGGCCAGGGCTACCTC
GATACGCCCGGCATCTACACCAAGGAACAAATCGAGGGCTGGCGCGCCGTCACTGACGAAGTGCATCGCCGCGGCGGCAA
GATCGTGCTGCAGCTGTGGCACGTTGGCCGCGTCTCGCACACCAGTGTGCTGCCGCCGGGCGAAGTGCCGGTTGCGCCCA
GCGCCATTCGCGCGGAAGGCAAGACCTTCACCAAGAACGGATTCGAGGACGTGTCCGAGCCGCGCGCGCTGGCATTGGAT
GAAATCCCCGGCTTGATCGAGGACTACCGCATCGCGGCGCGCAACGCGATCGATGCCGGCTTCGACGGCGTGGAAGTGCA
TGCGGCCAACGGCTATTTGTTGGACCAGTTCCTGCGCGACAGCTCCAACCAGCGCACCGACACCTACGGTGGCGATATCG
AAAACCGTACCCGCCTGCTTGCCGAAGTGGTGCAGGCGATTGCCGATGAAATCGGCGCCGACCGCACCGGCGTGCGCTTG
TCGCCGGTGACCCCGGTGTACGGCGCGCACGACTCCGACCCGCAGCCGCTGTTCGAACGTGCGGTCGAGCGCCTGAACCT
GATCGGCGGGCTGGCCTTCGTGCACGTGATCGAGGGCGCCACCGGCGGCCCGCGAGACAACATCGCCTTCGACTATGCCG
CGCTGCGCGCCAAGTTCGACGGCGCCTGGATCGCCAACAACGGCTACGACCGCGGCATGTCCGAACAGGCCATCAGCAGC
GGCTACGCCGATGCGATCGCCTATGGCCGCCCGTTCATCGCCAATCCGGACCTGGTGCGTCGCCTGCGCGAGAACGCGCC
GCTGGCCGAGATCGACCAGGCCACCATGTACGGCGGCGGTGCCAAGGGTTACACCGACTACCCCGCTTTGCCGGAGTAA

Upstream 100 bases:

>100_bases
GCGACGGGGACGCTGTGTCCTATCTACATCGCTCGAATCTGCTTGAGTCCGCCACCATCGCTACCGGGCACCGCGATCGG
CGCGGCGTCGCGCCTGGCGC

Downstream 100 bases:

>100_bases
TCGCCTGATCAGCCGCTGACCAGGCGACCGCACCTGCCGGCTGGCACGGTGCGGTTGCCGTGGGTGACGCCGATGCACTG
CATGCCTGCTGTTGGCCGAT

Product: putative oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MINFDWLLMSKSTQSPLFSPVRLGALELANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRATAGLIVAEGTQISPLGQGYL
DTPGIYTKEQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSHTSVLPPGEVPVAPSAIRAEGKTFTKNGFEDVSEPRALALD
EIPGLIEDYRIAARNAIDAGFDGVEVHAANGYLLDQFLRDSSNQRTDTYGGDIENRTRLLAEVVQAIADEIGADRTGVRL
SPVTPVYGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFDGAWIANNGYDRGMSEQAISS
GYADAIAYGRPFIANPDLVRRLRENAPLAEIDQATMYGGGAKGYTDYPALPE

Sequences:

>Translated_372_residues
MINFDWLLMSKSTQSPLFSPVRLGALELANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRATAGLIVAEGTQISPLGQGYL
DTPGIYTKEQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSHTSVLPPGEVPVAPSAIRAEGKTFTKNGFEDVSEPRALALD
EIPGLIEDYRIAARNAIDAGFDGVEVHAANGYLLDQFLRDSSNQRTDTYGGDIENRTRLLAEVVQAIADEIGADRTGVRL
SPVTPVYGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFDGAWIANNGYDRGMSEQAISS
GYADAIAYGRPFIANPDLVRRLRENAPLAEIDQATMYGGGAKGYTDYPALPE
>Mature_372_residues
MINFDWLLMSKSTQSPLFSPVRLGALELANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRATAGLIVAEGTQISPLGQGYL
DTPGIYTKEQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSHTSVLPPGEVPVAPSAIRAEGKTFTKNGFEDVSEPRALALD
EIPGLIEDYRIAARNAIDAGFDGVEVHAANGYLLDQFLRDSSNQRTDTYGGDIENRTRLLAEVVQAIADEIGADRTGVRL
SPVTPVYGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFDGAWIANNGYDRGMSEQAISS
GYADAIAYGRPFIANPDLVRRLRENAPLAEIDQATMYGGGAKGYTDYPALPE

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=369, Percent_Identity=46.6124661246612, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1789463, Length=334, Percent_Identity=32.0359281437126, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17565138, Length=359, Percent_Identity=29.8050139275766, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=271, Percent_Identity=30.9963099630996, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=269, Percent_Identity=29.368029739777, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=262, Percent_Identity=30.1526717557252, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=256, Percent_Identity=30.859375, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=261, Percent_Identity=28.3524904214559, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=383, Percent_Identity=37.8590078328982, Blast_Score=212, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6321973, Length=385, Percent_Identity=36.3636363636364, Blast_Score=197, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40248; Mature: 40248

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINFDWLLMSKSTQSPLFSPVRLGALELANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRA
CCCEEEEEEECCCCCCCCCHHHHHHHHHHCCEEECCCCCCCEECCCCCCCHHHHHCCCCC
TAGLIVAEGTQISPLGQGYLDTPGIYTKEQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSH
CCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
TSVLPPGEVPVAPSAIRAEGKTFTKNGFEDVSEPRALALDEIPGLIEDYRIAARNAIDAG
CCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCC
FDGVEVHAANGYLLDQFLRDSSNQRTDTYGGDIENRTRLLAEVVQAIADEIGADRTGVRL
CCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
SPVTPVYGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCC
GAWIANNGYDRGMSEQAISSGYADAIAYGRPFIANPDLVRRLRENAPLAEIDQATMYGGG
CEEEECCCCCCCCHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCHHHCCCCC
AKGYTDYPALPE
CCCCCCCCCCCC
>Mature Secondary Structure
MINFDWLLMSKSTQSPLFSPVRLGALELANRVIMAPLTRNRAVAGQVPSPLAAEYYGQRA
CCCEEEEEEECCCCCCCCCHHHHHHHHHHCCEEECCCCCCCEECCCCCCCHHHHHCCCCC
TAGLIVAEGTQISPLGQGYLDTPGIYTKEQIEGWRAVTDEVHRRGGKIVLQLWHVGRVSH
CCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
TSVLPPGEVPVAPSAIRAEGKTFTKNGFEDVSEPRALALDEIPGLIEDYRIAARNAIDAG
CCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCC
FDGVEVHAANGYLLDQFLRDSSNQRTDTYGGDIENRTRLLAEVVQAIADEIGADRTGVRL
CCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
SPVTPVYGAHDSDPQPLFERAVERLNLIGGLAFVHVIEGATGGPRDNIAFDYAALRAKFD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHCC
GAWIANNGYDRGMSEQAISSGYADAIAYGRPFIANPDLVRRLRENAPLAEIDQATMYGGG
CEEEECCCCCCCCHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCHHHCCCCC
AKGYTDYPALPE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]