| Definition | Xanthomonas campestris pv. vesicatoria str. 85-10 chromosome, complete genome. |
|---|---|
| Accession | NC_007508 |
| Length | 5,178,466 |
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The map label for this gene is lacZ [H]
Identifier: 78046915
GI number: 78046915
Start: 1531925
End: 1534489
Strand: Reverse
Name: lacZ [H]
Synonym: XCV1359
Alternate gene names: 78046915
Gene position: 1534489-1531925 (Counterclockwise)
Preceding gene: 78046916
Following gene: 78046912
Centisome position: 29.63
GC content: 65.89
Gene sequence:
>2565_bases ATGAGTGGTATCAATCGACGCGAGTTGCTGCGCGGCATGCTGGCCAGTGGGGTAAGCGCCGCACTGCCGATGGGCGCAGC CGGCACTTTGGCATCGCCTGGCGCGACGGCTGCACCTGCGGCCAAAAACACCGTATCGACAGCGGCATTAGGCGATGCAG CACTGGCGCCGCGGGAGCGTCTGCTGTTCGACTTCGGTTGGCGCTTCCACCTGGGCCATGCCAGCGATGCACCGCGCGAT TTCGAATTCGGCACCTTCCAACGCACCTTCGCCAAGGCCGGCAAGGACACCGCGACCGCCGCGCAACTGGCATTCGACGA CAGCGCCTGGCAGCAGGTGGACTTGCCGCACGACTGGGCCGTCGGTTTGCCATTTCGGCAGGAGCCGATCTCGGCCTTCA TGACCGAGGAAGACCCGGCCGCTGCACACGGCTACAAGCCGCTGGGCACGAGTTTCCCGGAGAACAGCGTGGGCTGGTAC CGGCGCACGCTGCAGATTCCGGCAAGCGATCTGGGCAAGCGCATCAGCCTGGTGTTGGATGGCGTGTTCCGTGAGTGCAT CGTGTTCTGCAATGGCCACATCGTCGGCCGTCACGCGAGCGGCTACTGCGGCTTCGAGGTCGACCTCAGCGAAGTGCTCG ACTACGGCAAGCCCAACATCATCGCCATCCGCGTGGATGCCACGCTGGGCGAAGGCTGGTTTTACGAAGGCGCCGGCATC TACCGGCATGTGTGGCTACAGAAGACCGACCCCCTCCACATTCCGCAAGACGGTGTGTTCGTACGCGGCAGCGTGCAGGA CGGCAACGCCACTGCGCAGCTCTCCACCGAAGTGCGCAACGACGGCACCGCGCCACGCCAGTGCGTGGTGCAGGCGCGCA TTGCCGCACCGGATGGACGCACCGTGGCGCAGGCGGCCAGCGCAGCGATCACGGTCGCGCCCGGCCAGGTGCAGGTGGTC GAACAGACCCTGCCGCTCGGGCAGGCGGCGCTGTGGTCGATCGACACCCCGCAGCTCTACAGCCTGACCACCAGCGTGCA CAGCGACGGCACCGCGCTCGATGCGCTGGTGACGCCGTTCGGCGTGCGCAGCATCGCCTTCGATGCGCAACGCGGTTTCC TGTTGAACGGCGCACCGCTCAAGCTGCATGGCACCAACAATCCCCAGGATCATGCCGGTGTCGGCACCGCCATTCCGGAC GCCTTGCACGCCTGGCGCCTGCGCCAGCTCAAGTCGATGGGCTGCAACGCGTATCGCAGCTCGCATAATCCGGCCACGCC GGAATTGCTCGCGCTGTGCGACCGCCTCGGCATGTTGGTGATCGAAGAAACCCGCCGCATGTCGACCGACCCCGAAGCGA TGGGCGAACTGGAAACGATGGTGCGGCGTGGCCGCAATCATCCCAGCGTCATCCTGTGGTCGCTCGGCAACGAAGAGCCG CAGCAAGTGACCGAGCGCGGTGCACGCATCGTGACCCGCATGCAACAACGCGTGCGCCAGCTCGACCCCACCCGCCCCAC CACCTTCGCGATGGACAAGGGCTTCGGTGATGGCGTGGGCCAGGTGGTGGATGTGGTCGGCTTCAACTACCGCACCAGCC AGATGGATGGCTTCCATGCGCAATATCCCCACATCCCGATCTACGGCAGCGAAACCGGCAGCACCGTGTCAGTGCGCGGC AACTATCAGCGCGACGACCAACGCGGCCATACCCGTGCCTACGACCTGGACCACCCCTGGTGGGCCAGCACCGCCGAAGC CTGGTGGAGCTATGTCGCGCAACGCCCGTACATTGCCGGCGGCTTCATCTGGACCGGTTTCGACTATCGCGGCGAGCCCA CGCCCTACAACCGCTGGCCGAATGTGGCTTCGCAATTCGGCGTACTCGATAGCTGCGGTTTTCCGAAAGACAACTACTGG CACTACCGCGCGCAGTGGACCAGCGAACCGGTACTGCATCTGTTCCCGCACTGGAACTGGGACGGCTTGCTGCAGCCCGA CGACAACGGCCGCATCGCGGTCTGGTGCCACAGCAATCTCGAGGCGGTGGAACTGCTGGTCAACGGCATCAGCCAGGGCT TGCAGCAAGTACCGGCTTACGGCCATGTCCAATGGCGTGTGGTCTATGCGCCCGGCATGATCGAAGCGCGCGGCTATCGC GGCGGCAAGCTGGTGCTGAGCGAGCGTCGCCAAACCACGGGCAAGCCGGCGGCGATTCGCTTGAGTTGCGATCGCAACGC GCTGCGTGCCGATGCCGAAGACGTCGCAGTGGTCAAGGTGGAAATCGTCGACGCACAAGGCCGCCTGGTACCGACTGCCG ACAACCTGGTGCAGTTCGCGCTGCGCGGACCTGCACGGCCGATTGGGGTCGGCAATGGCGACCCCAGCAGCCACGAAGAC GACAAGGCGCCGCGGCGCAAGGCGTTCAACGGCCTGTGTGCGGCGCTGCTGCAAACCACGCGCAGCAGCGGCGACATCGT GCTGCAGGCGACCGCGCCTGGGCTGACCGCATCCACGTTGCGCCTGCCTGCCGAGCCCACGCGATCGCGCGCGTCGGTGG CTTGA
Upstream 100 bases:
>100_bases ACCCGTATACCGGTGTGCCTGCATCGCGTGCATCCGACGCGGGCAAGCATGATGCGAGCGCGCCAGAGAGTTCTGTAGAT GCCGGCAAAAGGACGCAGCC
Downstream 100 bases:
>100_bases AACGCAGTCGCTGCGTACGGCAACGGAACGTGCTGCGTTACCTGGGCCAGTGGCGGCCTCGGTAGCGTCGCGATGCCATA CGCGAGTCGAAGGCCATCCA
Product: putative beta-galactosidase precursor
Products: NA
Alternate protein names: Beta-gal; Lactase [H]
Number of amino acids: Translated: 854; Mature: 853
Protein sequence:
>854_residues MSGINRRELLRGMLASGVSAALPMGAAGTLASPGATAAPAAKNTVSTAALGDAALAPRERLLFDFGWRFHLGHASDAPRD FEFGTFQRTFAKAGKDTATAAQLAFDDSAWQQVDLPHDWAVGLPFRQEPISAFMTEEDPAAAHGYKPLGTSFPENSVGWY RRTLQIPASDLGKRISLVLDGVFRECIVFCNGHIVGRHASGYCGFEVDLSEVLDYGKPNIIAIRVDATLGEGWFYEGAGI YRHVWLQKTDPLHIPQDGVFVRGSVQDGNATAQLSTEVRNDGTAPRQCVVQARIAAPDGRTVAQAASAAITVAPGQVQVV EQTLPLGQAALWSIDTPQLYSLTTSVHSDGTALDALVTPFGVRSIAFDAQRGFLLNGAPLKLHGTNNPQDHAGVGTAIPD ALHAWRLRQLKSMGCNAYRSSHNPATPELLALCDRLGMLVIEETRRMSTDPEAMGELETMVRRGRNHPSVILWSLGNEEP QQVTERGARIVTRMQQRVRQLDPTRPTTFAMDKGFGDGVGQVVDVVGFNYRTSQMDGFHAQYPHIPIYGSETGSTVSVRG NYQRDDQRGHTRAYDLDHPWWASTAEAWWSYVAQRPYIAGGFIWTGFDYRGEPTPYNRWPNVASQFGVLDSCGFPKDNYW HYRAQWTSEPVLHLFPHWNWDGLLQPDDNGRIAVWCHSNLEAVELLVNGISQGLQQVPAYGHVQWRVVYAPGMIEARGYR GGKLVLSERRQTTGKPAAIRLSCDRNALRADAEDVAVVKVEIVDAQGRLVPTADNLVQFALRGPARPIGVGNGDPSSHED DKAPRRKAFNGLCAALLQTTRSSGDIVLQATAPGLTASTLRLPAEPTRSRASVA
Sequences:
>Translated_854_residues MSGINRRELLRGMLASGVSAALPMGAAGTLASPGATAAPAAKNTVSTAALGDAALAPRERLLFDFGWRFHLGHASDAPRD FEFGTFQRTFAKAGKDTATAAQLAFDDSAWQQVDLPHDWAVGLPFRQEPISAFMTEEDPAAAHGYKPLGTSFPENSVGWY RRTLQIPASDLGKRISLVLDGVFRECIVFCNGHIVGRHASGYCGFEVDLSEVLDYGKPNIIAIRVDATLGEGWFYEGAGI YRHVWLQKTDPLHIPQDGVFVRGSVQDGNATAQLSTEVRNDGTAPRQCVVQARIAAPDGRTVAQAASAAITVAPGQVQVV EQTLPLGQAALWSIDTPQLYSLTTSVHSDGTALDALVTPFGVRSIAFDAQRGFLLNGAPLKLHGTNNPQDHAGVGTAIPD ALHAWRLRQLKSMGCNAYRSSHNPATPELLALCDRLGMLVIEETRRMSTDPEAMGELETMVRRGRNHPSVILWSLGNEEP QQVTERGARIVTRMQQRVRQLDPTRPTTFAMDKGFGDGVGQVVDVVGFNYRTSQMDGFHAQYPHIPIYGSETGSTVSVRG NYQRDDQRGHTRAYDLDHPWWASTAEAWWSYVAQRPYIAGGFIWTGFDYRGEPTPYNRWPNVASQFGVLDSCGFPKDNYW HYRAQWTSEPVLHLFPHWNWDGLLQPDDNGRIAVWCHSNLEAVELLVNGISQGLQQVPAYGHVQWRVVYAPGMIEARGYR GGKLVLSERRQTTGKPAAIRLSCDRNALRADAEDVAVVKVEIVDAQGRLVPTADNLVQFALRGPARPIGVGNGDPSSHED DKAPRRKAFNGLCAALLQTTRSSGDIVLQATAPGLTASTLRLPAEPTRSRASVA >Mature_853_residues SGINRRELLRGMLASGVSAALPMGAAGTLASPGATAAPAAKNTVSTAALGDAALAPRERLLFDFGWRFHLGHASDAPRDF EFGTFQRTFAKAGKDTATAAQLAFDDSAWQQVDLPHDWAVGLPFRQEPISAFMTEEDPAAAHGYKPLGTSFPENSVGWYR RTLQIPASDLGKRISLVLDGVFRECIVFCNGHIVGRHASGYCGFEVDLSEVLDYGKPNIIAIRVDATLGEGWFYEGAGIY RHVWLQKTDPLHIPQDGVFVRGSVQDGNATAQLSTEVRNDGTAPRQCVVQARIAAPDGRTVAQAASAAITVAPGQVQVVE QTLPLGQAALWSIDTPQLYSLTTSVHSDGTALDALVTPFGVRSIAFDAQRGFLLNGAPLKLHGTNNPQDHAGVGTAIPDA LHAWRLRQLKSMGCNAYRSSHNPATPELLALCDRLGMLVIEETRRMSTDPEAMGELETMVRRGRNHPSVILWSLGNEEPQ QVTERGARIVTRMQQRVRQLDPTRPTTFAMDKGFGDGVGQVVDVVGFNYRTSQMDGFHAQYPHIPIYGSETGSTVSVRGN YQRDDQRGHTRAYDLDHPWWASTAEAWWSYVAQRPYIAGGFIWTGFDYRGEPTPYNRWPNVASQFGVLDSCGFPKDNYWH YRAQWTSEPVLHLFPHWNWDGLLQPDDNGRIAVWCHSNLEAVELLVNGISQGLQQVPAYGHVQWRVVYAPGMIEARGYRG GKLVLSERRQTTGKPAAIRLSCDRNALRADAEDVAVVKVEIVDAQGRLVPTADNLVQFALRGPARPIGVGNGDPSSHEDD KAPRRKAFNGLCAALLQTTRSSGDIVLQATAPGLTASTLRLPAEPTRSRASVA
Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]
COG id: COG3250
COG function: function code G; Beta-galactosidase/beta-glucuronidase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 2 family [H]
Homologues:
Organism=Homo sapiens, GI268834192, Length=523, Percent_Identity=26.0038240917782, Blast_Score=132, Evalue=2e-30, Organism=Escherichia coli, GI48994920, Length=418, Percent_Identity=27.9904306220096, Blast_Score=122, Evalue=1e-28, Organism=Escherichia coli, GI1787903, Length=424, Percent_Identity=26.1792452830189, Blast_Score=109, Evalue=6e-25, Organism=Escherichia coli, GI1786539, Length=534, Percent_Identity=25.8426966292135, Blast_Score=109, Evalue=1e-24, Organism=Caenorhabditis elegans, GI17510825, Length=452, Percent_Identity=25.6637168141593, Blast_Score=112, Evalue=1e-24, Organism=Drosophila melanogaster, GI19922582, Length=426, Percent_Identity=27.9342723004695, Blast_Score=141, Evalue=2e-33, Organism=Drosophila melanogaster, GI62471735, Length=426, Percent_Identity=27.9342723004695, Blast_Score=141, Evalue=2e-33, Organism=Drosophila melanogaster, GI24655438, Length=426, Percent_Identity=27.9342723004695, Blast_Score=140, Evalue=3e-33, Organism=Drosophila melanogaster, GI24651745, Length=437, Percent_Identity=27.6887871853547, Blast_Score=134, Evalue=3e-31,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003344 - InterPro: IPR008979 - InterPro: IPR006101 - InterPro: IPR013812 - InterPro: IPR006104 - InterPro: IPR006102 - InterPro: IPR006103 - InterPro: IPR017853 - InterPro: IPR013781 [H]
Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]
EC number: =3.2.1.23 [H]
Molecular weight: Translated: 93177; Mature: 93045
Theoretical pI: Translated: 6.96; Mature: 6.96
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSGINRRELLRGMLASGVSAALPMGAAGTLASPGATAAPAAKNTVSTAALGDAALAPRER CCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCHHH LLFDFGWRFHLGHASDAPRDFEFGTFQRTFAKAGKDTATAAQLAFDDSAWQQVDLPHDWA HEEECCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHEEECCCCCCCCCCCCCCE VGLPFRQEPISAFMTEEDPAAAHGYKPLGTSFPENSVGWYRRTLQIPASDLGKRISLVLD ECCCCCCCCHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH GVFRECIVFCNGHIVGRHASGYCGFEVDLSEVLDYGKPNIIAIRVDATLGEGWFYEGAGI HHHHHHHHHHCCEEEEECCCCCCEEEEEHHHHHCCCCCCEEEEEEEEEECCCEEECCCCE YRHVWLQKTDPLHIPQDGVFVRGSVQDGNATAQLSTEVRNDGTAPRQCVVQARIAAPDGR EEEEEEECCCCCCCCCCCEEEEEECCCCCCEEEEEHHHCCCCCCCHHEEEEEEEECCCCC TVAQAASAAITVAPGQVQVVEQTLPLGQAALWSIDTPQLYSLTTSVHSDGTALDALVTPF HHHHHCCCEEEECCCCEEEEEHHCCCCCCEEEECCCCCEEEEEEHHCCCCCHHHHHHCCC GVRSIAFDAQRGFLLNGAPLKLHGTNNPQDHAGVGTAIPDALHAWRLRQLKSMGCNAYRS CHHHEEEECCCCEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHC SHNPATPELLALCDRLGMLVIEETRRMSTDPEAMGELETMVRRGRNHPSVILWSLGNEEP CCCCCCHHHHHHHHHHCCEEEEHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCH QQVTERGARIVTRMQQRVRQLDPTRPTTFAMDKGFGDGVGQVVDVVGFNYRTSQMDGFHA HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCC QYPHIPIYGSETGSTVSVRGNYQRDDQRGHTRAYDLDHPWWASTAEAWWSYVAQRPYIAG CCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEC GFIWTGFDYRGEPTPYNRWPNVASQFGVLDSCGFPKDNYWHYRAQWTSEPVLHLFPHWNW CEEEECCCCCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCEEEEEECCCCCEEEECCCCCC DGLLQPDDNGRIAVWCHSNLEAVELLVNGISQGLQQVPAYGHVQWRVVYAPGMIEARGYR CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCC GGKLVLSERRQTTGKPAAIRLSCDRNALRADAEDVAVVKVEIVDAQGRLVPTADNLVQFA CCEEEEECHHHCCCCCEEEEEECCCCHHHCCCCCEEEEEEEEEECCCCEECCHHHHHHHH LRGPARPIGVGNGDPSSHEDDKAPRRKAFNGLCAALLQTTRSSGDIVLQATAPGLTASTL HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEE RLPAEPTRSRASVA ECCCCCCCCCCCCC >Mature Secondary Structure SGINRRELLRGMLASGVSAALPMGAAGTLASPGATAAPAAKNTVSTAALGDAALAPRER CCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCHHH LLFDFGWRFHLGHASDAPRDFEFGTFQRTFAKAGKDTATAAQLAFDDSAWQQVDLPHDWA HEEECCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHEEECCCCCCCCCCCCCCE VGLPFRQEPISAFMTEEDPAAAHGYKPLGTSFPENSVGWYRRTLQIPASDLGKRISLVLD ECCCCCCCCHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH GVFRECIVFCNGHIVGRHASGYCGFEVDLSEVLDYGKPNIIAIRVDATLGEGWFYEGAGI HHHHHHHHHHCCEEEEECCCCCCEEEEEHHHHHCCCCCCEEEEEEEEEECCCEEECCCCE YRHVWLQKTDPLHIPQDGVFVRGSVQDGNATAQLSTEVRNDGTAPRQCVVQARIAAPDGR EEEEEEECCCCCCCCCCCEEEEEECCCCCCEEEEEHHHCCCCCCCHHEEEEEEEECCCCC TVAQAASAAITVAPGQVQVVEQTLPLGQAALWSIDTPQLYSLTTSVHSDGTALDALVTPF HHHHHCCCEEEECCCCEEEEEHHCCCCCCEEEECCCCCEEEEEEHHCCCCCHHHHHHCCC GVRSIAFDAQRGFLLNGAPLKLHGTNNPQDHAGVGTAIPDALHAWRLRQLKSMGCNAYRS CHHHEEEECCCCEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHC SHNPATPELLALCDRLGMLVIEETRRMSTDPEAMGELETMVRRGRNHPSVILWSLGNEEP CCCCCCHHHHHHHHHHCCEEEEHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCH QQVTERGARIVTRMQQRVRQLDPTRPTTFAMDKGFGDGVGQVVDVVGFNYRTSQMDGFHA HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCC QYPHIPIYGSETGSTVSVRGNYQRDDQRGHTRAYDLDHPWWASTAEAWWSYVAQRPYIAG CCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEC GFIWTGFDYRGEPTPYNRWPNVASQFGVLDSCGFPKDNYWHYRAQWTSEPVLHLFPHWNW CEEEECCCCCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCEEEEEECCCCCEEEECCCCCC DGLLQPDDNGRIAVWCHSNLEAVELLVNGISQGLQQVPAYGHVQWRVVYAPGMIEARGYR CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEECCCC GGKLVLSERRQTTGKPAAIRLSCDRNALRADAEDVAVVKVEIVDAQGRLVPTADNLVQFA CCEEEEECHHHCCCCCEEEEEECCCCHHHCCCCCEEEEEEEEEECCCCEECCHHHHHHHH LRGPARPIGVGNGDPSSHEDDKAPRRKAFNGLCAALLQTTRSSGDIVLQATAPGLTASTL HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEE RLPAEPTRSRASVA ECCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA