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The map label for this gene is gloB [H]

Identifier: 78046643

GI number: 78046643

Start: 1223336

End: 1224103

Strand: Reverse

Name: gloB [H]

Synonym: XCV1087

Alternate gene names: 78046643

Gene position: 1224103-1223336 (Counterclockwise)

Preceding gene: 78046647

Following gene: 78046642

Centisome position: 23.64

GC content: 65.23

Gene sequence:

>768_bases
ATGCGACTGATCGCCCTGCCCGCATTCGACGACAACTACATCTGGGCGCTGATCGCTGCAGATGGCCGCGCCATCATTGT
CGACCCCGGTCAGGCCGAGCCGGTCCTGGCTGCCGCGCAGCACCAGGGCCTGGTGCCGAGCGCCGTCCTGCTCACCCACC
ATCACGGCGACCATATCGGCGGCGTTGCCGAGCTCCAGCAGCGCTGGCCGGACCTGGAGTTGTTCGGCCCGGCCGACGAA
CGCATCCCGGCGGACGCGCATCAGGTAGGCCACGGCGAGCGCTTGCGCCTGCTCGATGTCGAGTTCCAGGTGCTCCAGGT
GCCCGGCCACACCCGCAGCCACATCGCTTTCGTCGCTGACGGCTACCTGTTCAGCGGTGATACCCTGTTCAGCCTAGGCT
GTGGGCGGATGTTCGAAGGTACCGCTCCCCAGATGTTCGACTCGTTGCAGCGCCTGGCCTCCCTGCCTGGCGAAACGCTT
GTGTGTTGCGGCCACGAATACACCTTGGCCAATGCGGCCTTCGCGCTGCACGTCGATCCCACCAACGCTGCCTTGCAGCG
CCGTCAACAGGAAGCCCAGGCCATGCGTCATGCAGCCCGTCCTACCCTGCCGATTTCGCTCAAGAGTGAACTGGCCACCA
ATCCGTTCTTACGCACCGGCCGCCCCGAGATCCGCGCTGCAGTGGCACCGCGCGCAGCCGGCGAGCTTTCCTCTGAAGTC
GATGTTTTCGCCGAACTTCGGCGCTGGAAAGACGAATTTCGCTCATGA

Upstream 100 bases:

>100_bases
GACGCTCCAGTTGAAGCAGGCCGCGCCCCGCGCCGGTTTCAAACCAGGATGAGACGCCAGCTTGACGTGAGAATGGCGGG
GCAGGCATGGTCCCAATTCT

Downstream 100 bases:

>100_bases
GGCGCCTGGTTTTGACAGTGCTGGCCGCCTGTTCGGCCATCGCACCGGCATCCGCATCGCCCAACGACGCAAGCTCCGCC
CCCGCCAGTAGCCAGGTCGC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MRLIALPAFDDNYIWALIAADGRAIIVDPGQAEPVLAAAQHQGLVPSAVLLTHHHGDHIGGVAELQQRWPDLELFGPADE
RIPADAHQVGHGERLRLLDVEFQVLQVPGHTRSHIAFVADGYLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETL
VCCGHEYTLANAAFALHVDPTNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTGRPEIRAAVAPRAAGELSSEV
DVFAELRRWKDEFRS

Sequences:

>Translated_255_residues
MRLIALPAFDDNYIWALIAADGRAIIVDPGQAEPVLAAAQHQGLVPSAVLLTHHHGDHIGGVAELQQRWPDLELFGPADE
RIPADAHQVGHGERLRLLDVEFQVLQVPGHTRSHIAFVADGYLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETL
VCCGHEYTLANAAFALHVDPTNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTGRPEIRAAVAPRAAGELSSEV
DVFAELRRWKDEFRS
>Mature_255_residues
MRLIALPAFDDNYIWALIAADGRAIIVDPGQAEPVLAAAQHQGLVPSAVLLTHHHGDHIGGVAELQQRWPDLELFGPADE
RIPADAHQVGHGERLRLLDVEFQVLQVPGHTRSHIAFVADGYLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETL
VCCGHEYTLANAAFALHVDPTNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTGRPEIRAAVAPRAAGELSSEV
DVFAELRRWKDEFRS

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=37.6425855513308, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=37.6425855513308, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI116642887, Length=266, Percent_Identity=34.9624060150376, Blast_Score=156, Evalue=1e-38,
Organism=Homo sapiens, GI21703352, Length=266, Percent_Identity=34.9624060150376, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI14150041, Length=233, Percent_Identity=38.1974248927039, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI46361987, Length=202, Percent_Identity=39.1089108910891, Blast_Score=133, Evalue=1e-31,
Organism=Escherichia coli, GI1786406, Length=253, Percent_Identity=45.4545454545455, Blast_Score=217, Evalue=5e-58,
Organism=Escherichia coli, GI1787158, Length=190, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=256, Percent_Identity=36.328125, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320478, Length=266, Percent_Identity=28.9473684210526, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324614, Length=269, Percent_Identity=29.368029739777, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356335, Length=265, Percent_Identity=37.7358490566038, Blast_Score=180, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24667711, Length=265, Percent_Identity=37.7358490566038, Blast_Score=180, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24667703, Length=265, Percent_Identity=37.7358490566038, Blast_Score=179, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 27914; Mature: 27914

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIALPAFDDNYIWALIAADGRAIIVDPGQAEPVLAAAQHQGLVPSAVLLTHHHGDHIG
CEEEEEECCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCC
GVAELQQRWPDLELFGPADERIPADAHQVGHGERLRLLDVEFQVLQVPGHTRSHIAFVAD
CHHHHHHHCCCEEEECCCCCCCCCCHHHCCCCCEEEEEEEEEEEEECCCCCCCEEEEEEC
GYLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETLVCCGHEYTLANAAFALHVDP
CEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCEEEEEEECC
TNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTGRPEIRAAVAPRAAGELSSEV
CHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCHHHHEEECCCHHHHHHHHH
DVFAELRRWKDEFRS
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLIALPAFDDNYIWALIAADGRAIIVDPGQAEPVLAAAQHQGLVPSAVLLTHHHGDHIG
CEEEEEECCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCC
GVAELQQRWPDLELFGPADERIPADAHQVGHGERLRLLDVEFQVLQVPGHTRSHIAFVAD
CHHHHHHHCCCEEEECCCCCCCCCCHHHCCCCCEEEEEEEEEEEEECCCCCCCEEEEEEC
GYLFSGDTLFSLGCGRMFEGTAPQMFDSLQRLASLPGETLVCCGHEYTLANAAFALHVDP
CEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCEEEEEEECC
TNAALQRRQQEAQAMRHAARPTLPISLKSELATNPFLRTGRPEIRAAVAPRAAGELSSEV
CHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCHHHHEEECCCHHHHHHHHH
DVFAELRRWKDEFRS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]