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The map label for this gene is gatA [H]

Identifier: 78046586

GI number: 78046586

Start: 1156361

End: 1158025

Strand: Reverse

Name: gatA [H]

Synonym: XCV1030

Alternate gene names: 78046586

Gene position: 1158025-1156361 (Counterclockwise)

Preceding gene: 78046587

Following gene: 78046583

Centisome position: 22.36

GC content: 67.93

Gene sequence:

>1665_bases
ATGCGATCCACCCTTTTGTCCTTCGCGCTGCTTGCCGCGCTCTGCGGCTGTGCGCATGCCGATGATCCGTCGCGTTCTGC
GGCCAGCGCTGCCGACGCGTCCACCGCCCACCCTCTCGATCTGAGCGAGGCCGACGTGGCAGGCCTGCAGGCGCGCATGG
CCAGCGGCCAGAGCAGCAGCCTGCAACTGACGCGCGCGTATCTGCAGCGTATTGCCGGCATCGATCGCGCCGGCCCCAGG
CTCAACGCGGTGATCGAACTCAATCCACAGGCCGAAGCCGACGCGCGCGCCCTGGATGCCGAACGCAAAGCCGGCCACGT
GCGTGGGCCGCTGCACGGCATCCCGGTGCTGCTCAAGGACAATATCGACGCGCTGCCGATGGTCAACAGCGCCGGCTCCC
TCGCCCTGGCCGAGTTCCACCCCGCTCGCGATGCGTTTGTGGTGCAGCGCCTGCGCACCGCCGGCGCGGTGATCCTGGGC
AAGACCAACCTCAGCGAATGGGCCAATTTCCGCTCCACCCAATCCAGTTCCGGCTGGAGCGGACGCGGAGGGCTCACGCG
CAATCCATATGCGCTGGATCGTAATCCCTGCGGCTCCAGTGCCGGCACCGGTGCGGCCATCGCGGCCAGCCTGGCCACCG
TCGGCATCGGGACCGAAACCGATGGCAGCATCACCTGTCCGGCATCGGTCAACGGCCTGGTCGGGCTCAAGCCGACCGTT
GGCCTGGTCAGTCGCGACGGCATCATCCCAATTTCCGCCAGTCAGGACACCGCCGGCCCGATGACCCGCAGCGTCGCCGA
TGCGGCTGCAGTGCTGCAGGCCATCGCCGCACCCGACCCGCAGGACCCCGCCACCGCCAAGGCACCGGCGACCAGCGTCG
ATTATCTCGCCTACCTGAAACCCGACAGCCTGCGTGGCGCGCGCCTGGGCCTGTTGCGCAACCCGCTGCGCGAGGATCCG
GCAATCGCCGCGGCGCTCGACCGCGCCGTGCGCACCCTGCGTGACGCTGGCGCCACCGTGGTCGAGACCGCCCTGGTCAC
CGATGGCAAGTGGGATGCTGCAGAGCAGATGGTCTTATTGGTCGAATTCAAGGCCGGCTTGAACGCGTATCTGCAGAACC
ACCACGCGCCTGTGGCGAACCTGGAGCAGTTGATTGCGTTCAATCGCAAGCACGCGCAGCGCGAAATGCCGTACTTCGGG
CAGGAATTGTTCGAACAGGCGCAGGCAGCGCCCGGTCTCGACGATGCGGGGTATCTGAGCGCGCGCGCCAACGCCAAGCG
ATTGGCGGGGCCGGAAGGCATCGACGCAGCCTTGAAGGCAGATCGCCTGGATGCCTTGATCGTGCCCACGACGGGCGCGG
CGTGGATCACCACACTCGGCAAGGGCGATACCTTCCCCGGCGCCGGCTATGGTGCCGCGGCGGTGGCCGGCTATCCAAGC
CTGAGCGTGCCGATGGGACAGACCCAGGGCTTGCCACTGGGCCTGTTGTTCATGGGCACCGCCTGGAGCGAGCCGCGCCT
GATCGAGCTGGCGTATGCATACGAGCAACGCAGCCATGCGCGCTTTACGCCTGGCTATGCGCCGAGTGCGCCGGCTGCGC
CAGGTGGGGCCACGTCAGGCACGATACCCGTCGCGCCTGCGCCCGGTTCGCACCAGCATGATTGA

Upstream 100 bases:

>100_bases
GCCGTCGCTGAGCCTGCCTGCCCGTTGGCGAGCACGTCCTTACGTTGTCACCCCGACGCAGCGCCGTTAGCCTGCGACTT
CTCCCCGGGGACGACCATCC

Downstream 100 bases:

>100_bases
CCGCCGTTATACCTGGCGCCCAAGAAGGATGCCCCGGCGGGTGCTTGTGCAACTGCCTGCCGAACACATTCGATGCCGGC
AGTTGCGCAATACCACCCGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MRSTLLSFALLAALCGCAHADDPSRSAASAADASTAHPLDLSEADVAGLQARMASGQSSSLQLTRAYLQRIAGIDRAGPR
LNAVIELNPQAEADARALDAERKAGHVRGPLHGIPVLLKDNIDALPMVNSAGSLALAEFHPARDAFVVQRLRTAGAVILG
KTNLSEWANFRSTQSSSGWSGRGGLTRNPYALDRNPCGSSAGTGAAIAASLATVGIGTETDGSITCPASVNGLVGLKPTV
GLVSRDGIIPISASQDTAGPMTRSVADAAAVLQAIAAPDPQDPATAKAPATSVDYLAYLKPDSLRGARLGLLRNPLREDP
AIAAALDRAVRTLRDAGATVVETALVTDGKWDAAEQMVLLVEFKAGLNAYLQNHHAPVANLEQLIAFNRKHAQREMPYFG
QELFEQAQAAPGLDDAGYLSARANAKRLAGPEGIDAALKADRLDALIVPTTGAAWITTLGKGDTFPGAGYGAAAVAGYPS
LSVPMGQTQGLPLGLLFMGTAWSEPRLIELAYAYEQRSHARFTPGYAPSAPAAPGGATSGTIPVAPAPGSHQHD

Sequences:

>Translated_554_residues
MRSTLLSFALLAALCGCAHADDPSRSAASAADASTAHPLDLSEADVAGLQARMASGQSSSLQLTRAYLQRIAGIDRAGPR
LNAVIELNPQAEADARALDAERKAGHVRGPLHGIPVLLKDNIDALPMVNSAGSLALAEFHPARDAFVVQRLRTAGAVILG
KTNLSEWANFRSTQSSSGWSGRGGLTRNPYALDRNPCGSSAGTGAAIAASLATVGIGTETDGSITCPASVNGLVGLKPTV
GLVSRDGIIPISASQDTAGPMTRSVADAAAVLQAIAAPDPQDPATAKAPATSVDYLAYLKPDSLRGARLGLLRNPLREDP
AIAAALDRAVRTLRDAGATVVETALVTDGKWDAAEQMVLLVEFKAGLNAYLQNHHAPVANLEQLIAFNRKHAQREMPYFG
QELFEQAQAAPGLDDAGYLSARANAKRLAGPEGIDAALKADRLDALIVPTTGAAWITTLGKGDTFPGAGYGAAAVAGYPS
LSVPMGQTQGLPLGLLFMGTAWSEPRLIELAYAYEQRSHARFTPGYAPSAPAAPGGATSGTIPVAPAPGSHQHD
>Mature_554_residues
MRSTLLSFALLAALCGCAHADDPSRSAASAADASTAHPLDLSEADVAGLQARMASGQSSSLQLTRAYLQRIAGIDRAGPR
LNAVIELNPQAEADARALDAERKAGHVRGPLHGIPVLLKDNIDALPMVNSAGSLALAEFHPARDAFVVQRLRTAGAVILG
KTNLSEWANFRSTQSSSGWSGRGGLTRNPYALDRNPCGSSAGTGAAIAASLATVGIGTETDGSITCPASVNGLVGLKPTV
GLVSRDGIIPISASQDTAGPMTRSVADAAAVLQAIAAPDPQDPATAKAPATSVDYLAYLKPDSLRGARLGLLRNPLREDP
AIAAALDRAVRTLRDAGATVVETALVTDGKWDAAEQMVLLVEFKAGLNAYLQNHHAPVANLEQLIAFNRKHAQREMPYFG
QELFEQAQAAPGLDDAGYLSARANAKRLAGPEGIDAALKADRLDALIVPTTGAAWITTLGKGDTFPGAGYGAAAVAGYPS
LSVPMGQTQGLPLGLLFMGTAWSEPRLIELAYAYEQRSHARFTPGYAPSAPAAPGGATSGTIPVAPAPGSHQHD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=519, Percent_Identity=26.0115606936416, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI195972892, Length=234, Percent_Identity=31.6239316239316, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=517, Percent_Identity=24.3713733075435, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=437, Percent_Identity=23.7986270022883, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=305, Percent_Identity=27.2131147540984, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=336, Percent_Identity=27.3809523809524, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=235, Percent_Identity=31.4893617021277, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=228, Percent_Identity=29.8245614035088, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=272, Percent_Identity=27.9411764705882, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=29.6370967741936, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24644968, Length=533, Percent_Identity=25.140712945591, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=27.7327935222672, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=27.7327935222672, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=27.7327935222672, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=27.7327935222672, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356731, Length=245, Percent_Identity=31.8367346938775, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648435, Length=315, Percent_Identity=28.2539682539683, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648437, Length=315, Percent_Identity=28.2539682539683, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45550774, Length=315, Percent_Identity=28.2539682539683, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648441, Length=233, Percent_Identity=30.9012875536481, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=233, Percent_Identity=30.9012875536481, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161078093, Length=260, Percent_Identity=30.3846153846154, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 57148; Mature: 57148

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSTLLSFALLAALCGCAHADDPSRSAASAADASTAHPLDLSEADVAGLQARMASGQSSS
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCH
LQLTRAYLQRIAGIDRAGPRLNAVIELNPQAEADARALDAERKAGHVRGPLHGIPVLLKD
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEC
NIDALPMVNSAGSLALAEFHPARDAFVVQRLRTAGAVILGKTNLSEWANFRSTQSSSGWS
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCC
GRGGLTRNPYALDRNPCGSSAGTGAAIAASLATVGIGTETDGSITCPASVNGLVGLKPTV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCEECCCCC
GLVSRDGIIPISASQDTAGPMTRSVADAAAVLQAIAAPDPQDPATAKAPATSVDYLAYLK
CEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEC
PDSLRGARLGLLRNPLREDPAIAAALDRAVRTLRDAGATVVETALVTDGKWDAAEQMVLL
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHEEEE
VEFKAGLNAYLQNHHAPVANLEQLIAFNRKHAQREMPYFGQELFEQAQAAPGLDDAGYLS
EEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
ARANAKRLAGPEGIDAALKADRLDALIVPTTGAAWITTLGKGDTFPGAGYGAAAVAGYPS
CCCCHHHCCCCCCCHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCHHHCCCCC
LSVPMGQTQGLPLGLLFMGTAWSEPRLIELAYAYEQRSHARFTPGYAPSAPAAPGGATSG
CCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCC
TIPVAPAPGSHQHD
EEEECCCCCCCCCC
>Mature Secondary Structure
MRSTLLSFALLAALCGCAHADDPSRSAASAADASTAHPLDLSEADVAGLQARMASGQSSS
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCH
LQLTRAYLQRIAGIDRAGPRLNAVIELNPQAEADARALDAERKAGHVRGPLHGIPVLLKD
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEC
NIDALPMVNSAGSLALAEFHPARDAFVVQRLRTAGAVILGKTNLSEWANFRSTQSSSGWS
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCC
GRGGLTRNPYALDRNPCGSSAGTGAAIAASLATVGIGTETDGSITCPASVNGLVGLKPTV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCEECCCCC
GLVSRDGIIPISASQDTAGPMTRSVADAAAVLQAIAAPDPQDPATAKAPATSVDYLAYLK
CEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEC
PDSLRGARLGLLRNPLREDPAIAAALDRAVRTLRDAGATVVETALVTDGKWDAAEQMVLL
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHEEEE
VEFKAGLNAYLQNHHAPVANLEQLIAFNRKHAQREMPYFGQELFEQAQAAPGLDDAGYLS
EEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
ARANAKRLAGPEGIDAALKADRLDALIVPTTGAAWITTLGKGDTFPGAGYGAAAVAGYPS
CCCCHHHCCCCCCCHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCHHHCCCCC
LSVPMGQTQGLPLGLLFMGTAWSEPRLIELAYAYEQRSHARFTPGYAPSAPAAPGGATSG
CCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCC
TIPVAPAPGSHQHD
EEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA