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The map label for this gene is gacS [H]

Identifier: 78046490

GI number: 78046490

Start: 1061456

End: 1063366

Strand: Reverse

Name: gacS [H]

Synonym: XCV0934

Alternate gene names: 78046490

Gene position: 1063366-1061456 (Counterclockwise)

Preceding gene: 78046491

Following gene: 78046489

Centisome position: 20.53

GC content: 65.78

Gene sequence:

>1911_bases
ATGAATGCATTGGCGGTGCGTTGGAACGATTGGCCGATTACCCGCAAAAGCCTGGCGGTGGTCACCCTGCCGCTGCTGTT
GCTGGCCGTTGCGTTGATGGCGATCTACAGCGTGGAACGGCAGAACATCGCCGCCGAAAACGACGTGCGGCAGACCCTGG
AAGTGCTCAGCGATCTGTATGAAGCGCACGCACTGTTGGCCGAAACCGCGGCCGGCGTGCGCGGATATCGGCTGGTGCGG
CGCGATGAATTCCTGACCCCGTATCGCGATGCCGAGCCGCGGCTGCAGCGCGTGGCCGACCGTCTTTCGCAACGCATCAC
CGATCCCAGCCAAGCGCGGCGCTTTGCGCGCATCAAGCCGCTTTTTGCGGAAAAAATGCAGGGTTGGCGCCGATTGCTGG
CACCGGATCTGAGCCGCGAAGAAGAGATCCGGCAACTGTGGGATGGCAAGTACAAACTCGACATCCTGCGTGCGGAACTG
CGCGAGTTGCGCAATTACGAAACCGCGCAGTTGCAGGTGCGTACCGCCAAGGCGCAAGGGCTGCGCCAGCGCAACCTCAT
CATCACCTTGAGCGCGGCGATGCTGGGCGGGCTGGGCGCGGTATTTGCGGTGGCATGGTTCGCCTCGGCATTGTCCGGGC
GCCTGCGCACCCTGGCCGCCAACGCCGACCGGCTGGGCGAAGGCCTGCCGCTGGCACCGCAGCCGCGTGCCGGCGACGAA
CTGGGCCAGGTCGGCCAGCGTCTGGCGCAAGCCAGCGAATTGTTGGCCGCACGCGCAGCCGAAGCGCAACTGGCGCGGCG
CGAGGCCGAAACCGCCAACCGCGCAAAAACCGAATTTCTCTCGCGCAGCAGCCATGAACTGCGGACTCCGCTCAATGCGA
TCCTGGGCTATGCGCAGGTGCTGGAAATGGATCTGCCCGAGCCGGGTCATCGGCGGCATCTGCAGCACATTCTGGGTGCC
GGCAGGCACTTGCTCGGGTTGATCACCGAGCTGCTGGACATCGCGCGTATCGAAGCCGATCAACTTGATCTGAGTCCGGA
GCCGGTCTCGGTACGCGCGGCCGTGCACGAAGCCCTGGGACTGATCGCACCGGATGCCGACAAGCATGGCATTGCGCTGC
AACCGGCCTCCATCGACGGACCGTGGACGGTGCGCGCCGATGCGCAGCGGCTGCGCCAGGTGCTGCTCAATCTGCTGTCG
AACGCGGTCAAGTTCAATCGTACTGGCGGGCAGGTGCAGGTGAGCGCGCAGGCCGATGGCGAACAGGTGCGTATTACGGT
TGCCGACCAGGGCGCGGGGCTGACCCCGGCGCAGATCGACCGCCTGTTTACACCGTTCGAGCGCTTGGGCGCCGAGCGCT
CTGCGGTGGAAGGCACCGGGCTGGGGTTGGCGCTGAGCAAGCGCTTGATCGAGGCGATGGGTGGACAGATCGGCGTGCAC
AGCAGCAGCGATGGCGCGCGGTTCTGGATTTCGCTGCCGCAGGGCGAGCATCGGCACAGTGAACCGGCGCGGCCGCTGGT
ACCGCCAAGCAGTGCCGGCAGCGGCGTGTGCCGGCTGTTGAGCATCGAAGACAATCCATCCAATCAGGCGTTGATCCGCA
CGCTCACCGAGCGTCGCCCGCAGTGGCAGCTGCTCGAGGCGGCCAGCCTGGCGCAGGCGCGTGCGCTATTGCAGCACGGC
GCGCCGGATCTGATCCTGCTGGACCTGCACCTAAGCGATGGCAACGGCGAAGAGCTGTTGCGCGAATTGCAGGCGCAGCC
GGCGCTGGCGGCCATCCCGGTGGTGGTGATCAGTGCCGATGCCACCACCGCCACACTGGCGCGCGTGGGCAATCAAGGCG
TGCGCGCCTATCTGACCAAGCCGCTCGACGTGGCCGAATTCTTCACCGTAATCGATGGGTTGCTGGCATGA

Upstream 100 bases:

>100_bases
CCGGCACGGTGAAAGTGCACGTGGAGCGAATCCTGCAGAAACTTGAGGTAAAAGATCGCACCCAGGCCGCGGTGTACGCG
GTCCAATCCGGACTTGCGCT

Downstream 100 bases:

>100_bases
CACGCGACGAAATTCTCGGTTCCCGCATCCTCATCGTCGACGACGAGCCGGCCAACGTGCGGTTGCTCGAAGACTTGCTG
CAACGCGAAGGTTTCCAGCA

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MNALAVRWNDWPITRKSLAVVTLPLLLLAVALMAIYSVERQNIAAENDVRQTLEVLSDLYEAHALLAETAAGVRGYRLVR
RDEFLTPYRDAEPRLQRVADRLSQRITDPSQARRFARIKPLFAEKMQGWRRLLAPDLSREEEIRQLWDGKYKLDILRAEL
RELRNYETAQLQVRTAKAQGLRQRNLIITLSAAMLGGLGAVFAVAWFASALSGRLRTLAANADRLGEGLPLAPQPRAGDE
LGQVGQRLAQASELLAARAAEAQLARREAETANRAKTEFLSRSSHELRTPLNAILGYAQVLEMDLPEPGHRRHLQHILGA
GRHLLGLITELLDIARIEADQLDLSPEPVSVRAAVHEALGLIAPDADKHGIALQPASIDGPWTVRADAQRLRQVLLNLLS
NAVKFNRTGGQVQVSAQADGEQVRITVADQGAGLTPAQIDRLFTPFERLGAERSAVEGTGLGLALSKRLIEAMGGQIGVH
SSSDGARFWISLPQGEHRHSEPARPLVPPSSAGSGVCRLLSIEDNPSNQALIRTLTERRPQWQLLEAASLAQARALLQHG
APDLILLDLHLSDGNGEELLRELQAQPALAAIPVVVISADATTATLARVGNQGVRAYLTKPLDVAEFFTVIDGLLA

Sequences:

>Translated_636_residues
MNALAVRWNDWPITRKSLAVVTLPLLLLAVALMAIYSVERQNIAAENDVRQTLEVLSDLYEAHALLAETAAGVRGYRLVR
RDEFLTPYRDAEPRLQRVADRLSQRITDPSQARRFARIKPLFAEKMQGWRRLLAPDLSREEEIRQLWDGKYKLDILRAEL
RELRNYETAQLQVRTAKAQGLRQRNLIITLSAAMLGGLGAVFAVAWFASALSGRLRTLAANADRLGEGLPLAPQPRAGDE
LGQVGQRLAQASELLAARAAEAQLARREAETANRAKTEFLSRSSHELRTPLNAILGYAQVLEMDLPEPGHRRHLQHILGA
GRHLLGLITELLDIARIEADQLDLSPEPVSVRAAVHEALGLIAPDADKHGIALQPASIDGPWTVRADAQRLRQVLLNLLS
NAVKFNRTGGQVQVSAQADGEQVRITVADQGAGLTPAQIDRLFTPFERLGAERSAVEGTGLGLALSKRLIEAMGGQIGVH
SSSDGARFWISLPQGEHRHSEPARPLVPPSSAGSGVCRLLSIEDNPSNQALIRTLTERRPQWQLLEAASLAQARALLQHG
APDLILLDLHLSDGNGEELLRELQAQPALAAIPVVVISADATTATLARVGNQGVRAYLTKPLDVAEFFTVIDGLLA
>Mature_636_residues
MNALAVRWNDWPITRKSLAVVTLPLLLLAVALMAIYSVERQNIAAENDVRQTLEVLSDLYEAHALLAETAAGVRGYRLVR
RDEFLTPYRDAEPRLQRVADRLSQRITDPSQARRFARIKPLFAEKMQGWRRLLAPDLSREEEIRQLWDGKYKLDILRAEL
RELRNYETAQLQVRTAKAQGLRQRNLIITLSAAMLGGLGAVFAVAWFASALSGRLRTLAANADRLGEGLPLAPQPRAGDE
LGQVGQRLAQASELLAARAAEAQLARREAETANRAKTEFLSRSSHELRTPLNAILGYAQVLEMDLPEPGHRRHLQHILGA
GRHLLGLITELLDIARIEADQLDLSPEPVSVRAAVHEALGLIAPDADKHGIALQPASIDGPWTVRADAQRLRQVLLNLLS
NAVKFNRTGGQVQVSAQADGEQVRITVADQGAGLTPAQIDRLFTPFERLGAERSAVEGTGLGLALSKRLIEAMGGQIGVH
SSSDGARFWISLPQGEHRHSEPARPLVPPSSAGSGVCRLLSIEDNPSNQALIRTLTERRPQWQLLEAASLAQARALLQHG
APDLILLDLHLSDGNGEELLRELQAQPALAAIPVVVISADATTATLARVGNQGVRAYLTKPLDVAEFFTVIDGLLA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87081816, Length=387, Percent_Identity=34.8837209302326, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1789149, Length=350, Percent_Identity=29.4285714285714, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI48994928, Length=368, Percent_Identity=30.7065217391304, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI145693157, Length=228, Percent_Identity=35.9649122807018, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788713, Length=447, Percent_Identity=27.5167785234899, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786912, Length=263, Percent_Identity=34.2205323193916, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790436, Length=212, Percent_Identity=33.0188679245283, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1788393, Length=272, Percent_Identity=31.6176470588235, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788549, Length=240, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786783, Length=223, Percent_Identity=26.9058295964126, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790346, Length=265, Percent_Identity=30.5660377358491, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=29.5154185022026, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87082128, Length=315, Percent_Identity=26.984126984127, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790861, Length=302, Percent_Identity=28.1456953642384, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787894, Length=235, Percent_Identity=28.936170212766, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1788279, Length=220, Percent_Identity=25.9090909090909, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=232, Percent_Identity=25, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 69451; Mature: 69451

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALAVRWNDWPITRKSLAVVTLPLLLLAVALMAIYSVERQNIAAENDVRQTLEVLSDLY
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EAHALLAETAAGVRGYRLVRRDEFLTPYRDAEPRLQRVADRLSQRITDPSQARRFARIKP
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LFAEKMQGWRRLLAPDLSREEEIRQLWDGKYKLDILRAELRELRNYETAQLQVRTAKAQG
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHEEEEEHHHHC
LRQRNLIITLSAAMLGGLGAVFAVAWFASALSGRLRTLAANADRLGEGLPLAPQPRAGDE
CHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHH
LGQVGQRLAQASELLAARAAEAQLARREAETANRAKTEFLSRSSHELRTPLNAILGYAQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LEMDLPEPGHRRHLQHILGAGRHLLGLITELLDIARIEADQLDLSPEPVSVRAAVHEALG
HHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHH
LIAPDADKHGIALQPASIDGPWTVRADAQRLRQVLLNLLSNAVKFNRTGGQVQVSAQADG
CCCCCCCCCCEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCC
EQVRITVADQGAGLTPAQIDRLFTPFERLGAERSAVEGTGLGLALSKRLIEAMGGQIGVH
CEEEEEEECCCCCCCHHHHHHHHCCHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCEECC
SSSDGARFWISLPQGEHRHSEPARPLVPPSSAGSGVCRLLSIEDNPSNQALIRTLTERRP
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCC
QWQLLEAASLAQARALLQHGAPDLILLDLHLSDGNGEELLRELQAQPALAAIPVVVISAD
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHCCCEEEEECC
ATTATLARVGNQGVRAYLTKPLDVAEFFTVIDGLLA
CHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNALAVRWNDWPITRKSLAVVTLPLLLLAVALMAIYSVERQNIAAENDVRQTLEVLSDLY
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EAHALLAETAAGVRGYRLVRRDEFLTPYRDAEPRLQRVADRLSQRITDPSQARRFARIKP
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LFAEKMQGWRRLLAPDLSREEEIRQLWDGKYKLDILRAELRELRNYETAQLQVRTAKAQG
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHEEEEEHHHHC
LRQRNLIITLSAAMLGGLGAVFAVAWFASALSGRLRTLAANADRLGEGLPLAPQPRAGDE
CHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHH
LGQVGQRLAQASELLAARAAEAQLARREAETANRAKTEFLSRSSHELRTPLNAILGYAQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LEMDLPEPGHRRHLQHILGAGRHLLGLITELLDIARIEADQLDLSPEPVSVRAAVHEALG
HHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHH
LIAPDADKHGIALQPASIDGPWTVRADAQRLRQVLLNLLSNAVKFNRTGGQVQVSAQADG
CCCCCCCCCCEEEECCCCCCCEEECHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCC
EQVRITVADQGAGLTPAQIDRLFTPFERLGAERSAVEGTGLGLALSKRLIEAMGGQIGVH
CEEEEEEECCCCCCCHHHHHHHHCCHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCEECC
SSSDGARFWISLPQGEHRHSEPARPLVPPSSAGSGVCRLLSIEDNPSNQALIRTLTERRP
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCC
QWQLLEAASLAQARALLQHGAPDLILLDLHLSDGNGEELLRELQAQPALAAIPVVVISAD
CHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHCCCEEEEECC
ATTATLARVGNQGVRAYLTKPLDVAEFFTVIDGLLA
CHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]